Article
Multidisciplinary Sciences
Thomas P. Reber, Sina Mackay, Marcel Bausch, Marcel S. Kehl, Valeri Borger, Rainer Surges, Florian Mormann
Summary: A central function of the human brain is to adapt to new situations based on past experience. This adaptation is reflected in behavioral and neurophysiological changes. In this study, the researchers used an adaptation paradigm with visual stimuli to explore potential single-neuron mechanisms underlying these changes. By simultaneously recording intracranial EEG and single-neuron spiking activity, they found a sharpening of single-neuron tuning curves in the amygdala, but an overall reduction of activity in the hippocampus, entorhinal cortex, and parahippocampal cortex.
NATURE COMMUNICATIONS
(2023)
Article
Developmental Biology
Matthieu X. Moreau, Yoann Saillour, Andrzej W. Cwetsch, Alessandra Pierani, Frederic Causeret
Summary: This study utilized single-cell RNAseq to uncover the diversity of progenitors and neurons along the dorso-ventral axis of the developing pallium. The data suggest that discrete neuronal fate acquisition from a continuous gradient of progenitors results from the superimposition of spatial information and temporal maturation.
Article
Multidisciplinary Sciences
Xiangbin Ruan, Bowei Kang, Cai Qi, Wenhe Lin, Jingshu Wang, Xiaochang Zhang
Summary: The study found that temporal gene expression in neural progenitors in the developing mouse neocortex is related to neuron type specification, supporting that neuronal identity genes are transcriptionally evident in Eomes-positive cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Neurosciences
Rui Mao, Xiaoyun Zhang, Youyong Kong, Shanshan Wu, Hai-qin Huo, Yue Kong, Zhen Wang, Yan Liu, Zhengping Jia, Zikai Zhou
Summary: This study reveals the important role of ALK in neurodevelopment, as transient embryonic ALK inactivation can affect the structure and function of the adult brain. Through scRNA-seq, it was found that ALK influences differentially expressed genes at different developmental stages, many of which are associated with neurological or neuropsychiatric disorders.
Article
Biochemistry & Molecular Biology
Piergiorgio La Rosa, Giulia Bartoli, Stefano Farioli Vecchioli, Eleonora Cesari, Vittoria Pagliarini, Claudio Sette
Summary: This study investigated the role of dihydrotestosterone (DHT) in regulating neural progenitor cell (NPC) stemness and differentiation, revealing that DHT activates androgen receptor (AR) to increase NPC proliferation and reduce neuronal differentiation. The findings suggest that androgens engage an AR-dependent signaling pathway to impact neurogenesis by expanding the NPC pool in the developing mouse cortex.
JOURNAL OF NEUROCHEMISTRY
(2021)
Review
Cell Biology
Flaminia Kaluthantrige Don, Nereo Kalebic
Summary: The expansion of the cerebral cortex is closely related to the acquisition of higher intellectual abilities that distinguish humans from their closest relatives. Recent studies using brain organoids have provided valuable insights into the molecular and cell biological features of basal radial glia (bRG), which can help understand the onset of neurodevelopmental disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Neurosciences
Shukui Zhang, Jinyue Zhao, Cheng Zhao, Libo Su, Jianwei Jiao
Summary: Regulation of neural stem cell behavior and neuronal development involves the BCAT1 gene, which is highly expressed in specific layers of the cerebral cortex and is involved in glutamate metabolism. Using single-cell data, researchers identified seven different states of neural stem cells, with state 4 being closely associated with the development of projection neurons. Suppression of BCAT1 resulted in reduced proliferation of neural stem cells and abnormal development of specific neuronal subtypes.
Article
Biology
Maribel Patino, Willian N. Lagos, Neelakshi S. Patne, Paula A. Miyazaki, Sai Krishna Bhamidipati, Forrest Collman, Edward M. Callaway
Summary: Retrograde monosynaptic tracing is an important tool for studying neural circuit structure and connectivity. This study used a glycoprotein-deleted rabies virus to label synaptic contacts in the mouse primary visual cortex and found similar proportions of labeled contacts across different cell types and subcellular locations.
Article
Cell & Tissue Engineering
Katie Williams, Simote T. Foliaki, Brent Race, Anna Smith, Tina Thomas, Bradley R. Groveman, Cathryn L. Haigh
Summary: This study investigated the impact of cell therapy on sporadic Creutzfeldt-Jakob disease (sCJD) using a human cerebral organoid model. The results showed that while the treatment had minimal effects on prions, it had a beneficial impact on organoid function and restored electrophysiological parameters to normal levels. This suggests that cell therapy may have functional benefits in the treatment of human prion diseases.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell Biology
Mengtian Zhang, Libo Su, Wenwen Wang, Chenxiao Li, Qingli Liang, Fen Ji, Jianwei Jiao
Summary: The intimate communication between vascular and nervous systems is crucial for CNS development. In this study, it was found that during the development of vascular endothelial cells (ECs), DNA damage occurs. The E3 ubiquitin ligase RNF20 is required for the DNA damage response. Deletion of RNF20 leads to accumulation of DNA damage in ECs, impairing the secretion of cartilage intermediate layer protein 2 (CILP2). The loss of endothelium-derived CILP2 disrupts the downstream signaling of the Wnt pathway through interaction with Wnt3a, causing disturbances in NPC fate and autism-like behaviors in mice.
Article
Cell Biology
Anna Konturek-Ciesla, Parashar Dhapola, Qinyu Zhang, Petter Sawen, Haixia Wan, Goran Karlsson, David Bryder
Summary: Aging negatively impacts hematopoiesis, leading to compromised immunity and blood disorders. By tracing the lineage and analyzing single-cell transcriptomes, the researchers found that aging leads to reduced lymphoid output through a specific type of progenitor cell. However, culturing certain aged hematopoietic stem cells can restore lymphopoiesis, offering potential strategies to improve immune function in the elderly.
Article
Multidisciplinary Sciences
Katharina Lust, Ashley Maynard, Tomas Gomes, Jonas Simon Fleck, J. Gray Camp, Elly M. Tanaka, Barbara Treutlein
Summary: This study used single-cell genomic profiling to investigate the cell populations in the axolotl brain, revealing similarities to neurons in other amniotes. The research also provided insights into the transcriptional dynamics and gene regulatory relationships during regeneration, as well as identifying conserved differentiation features.
Article
Neurosciences
Ruth Benavides-Piccione, Concepcion Rojo, Asta Kastanauskaite, Javier DeFelipe
Summary: Pyramidal neurons, the most abundant and characteristic type in the cerebral cortex, have been found to exhibit regional differences in morphology within the human cortex. While there are some conserved aspects of pyramidal structure, specific variations across cortical regions and species suggest that structural diversity exists.
Article
Multidisciplinary Sciences
Owen H. Funk, Yaman Qalieh, Daniel Z. Doyle, Mandy M. Lam, Kenneth Y. Kwan
Summary: The histone variant H3.3 plays crucial roles in neuronal development. Newly born cortical excitatory neurons accumulate H3.3 immediately postmitosis, and deletion of H3.3-encoding genes disrupts the establishment of the neuronal transcriptome.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Alexandra Pokhilko, Adam E. Handel, Fabiola Curion, Viola Volpato, Emma S. Whiteley, Sunniva Bostrand, Sarah E. Newey, Colin J. Akerman, Caleb Webber, Michael B. Clark, Rory Bowden, M. Zameel Cader
Summary: Targeted sequencing of TFs using scCapture-seq in iPSC-derived neuronal cultures enhances biological information obtained from scRNA-seq, improving cell type identification, developmental trajectories, and gene regulatory networks. This approach also reveals subtle differences and development divergence among neuronal populations.
Review
Neurosciences
Ayano Kawaguchi
NEUROSCIENCE RESEARCH
(2019)
Article
Developmental Biology
Kazuaki Maruyama, Sachiko Miyagawa-Tomita, Kaoru Mizukami, Fumio Matsuzaki, Hiroki Kurihara
DEVELOPMENTAL BIOLOGY
(2019)
Article
Developmental Biology
Daijiro Konno, Chiaki Kishidal, Kazumitsu Maehara, Yasuyuki Ohkawa, Hiroshi Kiyonari, Seiji Okada, Fumio Matsuzaki
Article
Multidisciplinary Sciences
T. Kawaue, A. Shitamukai, A. Nagasaka, Y. Tsunekawa, T. Shinoda, K. Saito, R. Terada, M. Bilgic, T. Miyata, F. Matsuzaki, A. Kawaguchi
NATURE COMMUNICATIONS
(2019)
Article
Anatomy & Morphology
Takako Kikkawa, Nobuyuki Sakayori, Hayato Yuuki, Yu Katsuyama, Fumio Matsuzaki, Daijiro Konno, Takaya Abe, Hiroshi Kiyonari, Noriko Osumi
DEVELOPMENTAL DYNAMICS
(2020)
Article
Cell Biology
Ikumi Fujita, Atsunori Shitamukai, Fumiya Kusumoto, Shun Mase, Taeko Suetsugu, Ayaka Omori, Kagayaki Kato, Takaya Abe, Go Shioi, Daijiro Konno, Fumio Matsuzaki
NATURE CELL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Shinji Hirotsune, Hiroshi Kiyonari, Mingyue Jin, Kanako Kumamoto, Kayo Yoshida, Miki Shinohara, Hitomi Watanabe, Anthony Wynshaw-Boris, Fumio Matsuzaki
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Naoya Gotoh, Yuhki Saito, Saori Hata, Haruka Saito, Daiki Ojima, Chiaki Murayama, Mayo Shigeta, Takaya Abe, Daijiro Konno, Fumio Matsuzaki, Toshiharu Suzuki, Tohru Yamamoto
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Yuki Hattori, Yu Naito, Yoji Tsugawa, Shigenori Nonaka, Hiroaki Wake, Takashi Nagasawa, Ayano Kawaguchi, Takaki Miyata
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Roie Cohen, Liat Amir-Zilberstein, Micha Hersch, Shiran Woland, Olga Loza, Shahar Taiber, Fumio Matsuzaki, Sven Bergmann, Karen B. Avraham, David Sprinzak
NATURE COMMUNICATIONS
(2020)
Article
Neurosciences
Shun Mase, Atsunori Shitamukai, Quan Wu, Mitsuru Morimoto, Thomas Gridley, Fumio Matsuzaki
Summary: Notch signaling is essential for maintaining neural stem cells and cortical architecture during mammalian corticogenesis. Single knockout of Notch1 and Notch2 genes have different impacts on RGC maintenance, while their conditional double knockout results in the complete loss of RGCs. These findings suggest a cooperative function of Notch1 and Notch2 in regulating RGCs during different stages of corticogenesis.
NEUROSCIENCE RESEARCH
(2021)
Review
Cell Biology
Ayano Kawaguchi
Summary: During neocortical development, the delamination of neurogenic cells is crucial for their migration, with the proper timing of detachment of the apical endfeet being critical for neurogenesis. Various molecular mechanisms, including the regulation of cell adhesion and cytoskeletal architecture, contribute to neurogenic cell delamination and potentially the generation of other types of neural progenitor cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Quan Wu, Yuichi Shichino, Takaya Abe, Taeko Suetsugu, Ayaka Omori, Hiroshi Kiyonari, Shintaro Iwasaki, Fumio Matsuzaki
Summary: This study reveals that Fbl, a ribosomal RNA methyltransferase, controls translational efficiencies of epigenetic modifiers during neural stem cell (NSC) development, thereby playing a potential role as a clock in the development of the cerebral cortex.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Kazuaki Maruyama, Sachiko Miyagawa-Tomita, Yuka Haneda, Mayuko Kida, Fumio Matsuzaki, Kyoko Imanaka-Yoshida, Hiroki Kurihara
Summary: Lymphatic vessels play a crucial role in maintaining tissue homeostasis and immune responses in vertebrates. Recent studies have identified two major sources of lymphatic endothelial cells (LECs): venous sprouting (lymphangiogenesis) and de novo production from non-venous origins (lymphvasculogenesis). This research using genetic lineage tracing in mice reveals that a subset of cardiopharyngeal mesoderm cells, known as Isl1-expressing cells, give rise to LECs in the craniofacial and cardiac regions. These findings enhance our understanding of lymphatic vessel development and may provide insights into the pathogenesis of lymphatic malformations.
Article
Developmental Biology
Akane Hatsuda, Junko Kurisu, Kazuto Fujishima, Ayano Kawaguchi, Nobuhiko Ohno, Mineko Kengaku
Summary: Dendritic outgrowth in immature neurons is enhanced by neuronal activity and calcium signals. Our study demonstrates that activity-dependent calcium signaling controls mitochondrial homeostasis through AMP-activated protein kinase (AMPK). AMPK activity is finely tuned by the calcium-CaMKK2 pathway and regulates mitochondrial dynamics in growing dendrites of differentiating neurons.
