Article
Biochemistry & Molecular Biology
Klaudia Tutka, Magdalena Zychowska, Anna Zaczek, Karolina Maternia-Dudzik, Jakub Pawelczyk, Dominik Strapagiel, Jakub Lach, Adam Reich
Summary: This study evaluated the diversity and composition of the bacterial microbiome in PN lesions and found that the abundance of Staphylococcus was significantly higher in samples from PN and AD patients compared to controls. The microbiome composition in PN was highly similar to that of AD. These findings suggest a potential role of the microbiome in the pathogenesis of PN.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Yuntian Xin, Jingxian Xie, Bingru Nan, Chen Tang, Yunshan Xiao, Quanfeng Wu, Yi Lin, Xueqin Zhang, Heqing Shen
Summary: This study aimed to improve the efficiency of bacterial DNA extraction from meconium, a challenging process due to low microflora levels and PCR inhibitors. Using freeze-thawing cycles and different freezing conditions, the researchers found that freezing at -20 degrees Celsius was the best condition for DNA extraction and preservation of microbial diversity in meconium. The developed protocol could significantly enhance DNA extraction efficiency and preserve the species diversity of meconium microflora, providing potential benefits for newborn bacterial colonization studies.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biodiversity Conservation
Barbara Zapala, Justyna Pustelnik, Alicja Dudek, Tomasz Milewicz
Summary: The gastrointestinal microbiota, consisting of trillions of microorganisms, is influenced by lifestyle and diet. A study on healthy Polish individuals following a Western diet found stable oral and gut microbiota profiles, with variations between genders.
Article
Immunology
Bo Zhang, Matthew Brock, Carlos Arana, Chaitanya Dende, Nicolai Stanislas van Oers, Lora Hooper, Prithvi Raj
Summary: Bead-beating is critical for DNA extraction, influencing the accurate assessment of microbial community composition. Full length 16S rRNA gene sequencing revealed species diversity in mouse and human gut microbiomes, as well as different levels of sensitivity to bead-beating treatment.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Jing Liu, Kelsy Robinson, Wentao Lyu, Qing Yang, Jing Wang, Karen D. Christensen, Guolong Zhang
Summary: The intestinal microbiota plays a crucial role in nutrient digestion, pathogen exclusion, immune development, and animal productivity. However, the specific bacteria associated with animal growth are still unknown. This study identified bacteria in the cecal microbiota that are linked to body weight gain in broiler chickens. These bacteria, which show either a positive or negative association with body weight, could be further studied to improve chicken growth performance and serve as potential biomarkers for selecting broiler chickens with different growth rates.
Article
Microbiology
Alba Boix-Amoros, Enrica Piras, Kevin Bu, David Wallach, Matthew Stapylton, Ana Fernandez-Sesma, Dolores Malaspina, Jose C. Clemente
Summary: Different inactivation methods have tissue-specific effects on the microbiome composition and diversity of samples collected from various body sites. Nasal and skin samples are most affected, while oral and stool microbiomes are more robust. Careful consideration should be given to the choice of inactivation method based on sample type.
Article
Environmental Sciences
Jessica L. Bergman, Tayla Shaw, Suhelen Egan, Tracy D. Ainsworth
Summary: With advancements in sequencing techniques, there has been a rapid increase in studies on the microbiome in coral health and response to environmental change. However, methodological variations in DNA extraction protocols and tissue sampling can impact downstream analyses of microbial communities. Different studies have focused on whole organisms or specific microhabitats within coral, leading to variations in microbial data generated.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Microbiology
Gertrude Wegl, Nikolaus Grabner, Andreas Kostelbauer, Viviana Klose, Mahdi Ghanbari
Summary: Understanding the roles of microorganisms in the animal gastrointestinal microenvironment is crucial for managing and manipulating microbial communities. A systematic comparison of sample preservation and DNA extraction approaches for pig and chicken microbiota samples showed that these technical steps can significantly affect microbial community structure, with effects varying by sample type. Standardization is essential for accelerating progress in livestock microbiota research.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Evgeny Abakumov, Aleksei Zverev, Evgeny Andronov, Timur Nizamutdinov
Summary: Technogenic processes and agrodevelopment cause significant changes in soil properties. Next-generation sequencing allows detailed investigation of soil microbial composition and functions. This study explored the taxonomic diversity of natural and anthropogenically disturbed soils in Russia using next-generation sequencing. It was found that microbial communities in disturbed soils differ from natural and agrogenic soils, indicating the sensitivity of microbial communities as indicators of human impact on soil.
APPLIED SCIENCES-BASEL
(2023)
Review
Nutrition & Dietetics
Lisa F. Stinson, Jie Ma, Azhar S. Sindi, Donna T. Geddes
Summary: Human milk microbiome research faces technical challenges such as sample collection, DNA extraction and sequencing, viability, and contamination. This study analyzes various methodological approaches to provide recommendations for robust and reproducible analysis of the human milk microbiome.
Review
Oncology
Carolina Pinto da Costa, Patricia Vieira, Melissa Mendes-Rocha, Joana Pereira-Marques, Rui Manuel Ferreira, Ceu Figueiredo
Summary: The relationship between the microbiome and colorectal cancer has been extensively studied, but little is known about the microbial community in intestinal tumor tissues. This systematic review compiled evidence on the relationship between tissue-associated microbiota and colorectal cancer and identified several bacterial groups that are strongly associated with the disease.
Article
Biotechnology & Applied Microbiology
A. S. Cheema, L. F. Stinson, C. T. Lai, D. T. Geddes, M. S. Payne
Summary: Evaluation of four DNA extraction methods revealed that kit QM is the most suitable for extracting bacterial DNA from human milk, as it was able to detect DNA in all samples with higher efficiency and reliability compared to other methods. The choice of extraction method significantly impacts the efficiency and bacterial community profiles obtained from human milk samples.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Microbiology
Luis Gonzalez, Jose Luis Paredes Sosa, Susan Mosquito, Yesenia Filio, Pedro E. Romero, Theresa J. Ochoa, Pablo Tsukayama
Summary: This study analyzed the gut microbiota composition of 60 toddlers aged 12-18 months who received daily administration of lactoferrin or placebo for 6 months. The results showed that lactoferrin treatment does not increase gut microbiota diversity over time nor affect its bacterial composition compared to the placebo group.
MICROBIOLOGY SPECTRUM
(2023)
Review
Biochemistry & Molecular Biology
Aleksandra Stupak, Wojciech Kwasniewski
Summary: The microbiome is of interest due to its potential influence on human illnesses. This review focuses on the methodologies and understanding of the functional microbiome in pregnancy outcomes. Research suggests that microbiome dysbiosis may be associated with antenatal illnesses such as fetal growth restriction and preeclampsia.
Article
Nutrition & Dietetics
Adelaide Teofani, Irene Marafini, Federica Laudisi, Daniele Pietrucci, Silvia Salvatori, Valeria Unida, Silvia Biocca, Giovanni Monteleone, Alessandro Desideri
Summary: This study investigated the impact of lifestyle and dietary habits on the intestinal microbiota diversity and relative abundance in patients with IBD, identifying specific confounders and covariates that influence the association between certain bacterial taxa and disease status. The results suggest that variables related to an IBD-associated lifestyle and dietary habits play a role in modulating the gut microbiota in IBD patients compared to healthy controls.
Article
Cardiac & Cardiovascular Systems
Ellen G. Avery, Hendrik Bartolomaeus, Ariana Rauch, Chia-Yu Chen, Gabriele N'Diaye, Ulrike Loeber, Theda U. P. Bartolomaeus, Raphaela Fritsche-Guenther, Andre F. Rodrigues, Alex Yarritu, Cheng Zhong, Lingyan Fei, Dmitry Tsvetkov, Mihail Todiras, Joon-Keun Park, Lajos Marko, Andras Maifeld, Andreas Patzak, Michael Bader, Stefan Kempa, Jennifer A. Kirwan, Sofia K. Forslund, Dominik N. Mueller, Nicola Wilck
Summary: This study investigated the impact of microbial colonization on organ damage in hypertension using germ-free and colonized mice models. The results showed that the kidney is more sensitive to microbial influence, and the colonization status is associated with circulating metabolites and inflammatory cells relevant to hypertension.
CARDIOVASCULAR RESEARCH
(2023)
Review
Cardiac & Cardiovascular Systems
Zeinab Ghorbani, Asma Kazemi, Theda U. P. Bartolomaeus, Fahimeh Martami, Morvarid Noormohammadi, Arsalan Salari, Ulrike Loeber, Heydar Ali Balou, Sofia K. Forslund, Marjan Mahdavi-Roshan
Summary: This study conducted a systematic literature search and meta-analysis to determine the effect sizes of probiotics on blood lipid parameters. The results showed that probiotic/synbiotic supplements can significantly decrease triglyceride, total cholesterol, and LDL-C levels, and increase HDL-C levels. These effects are more pronounced in individuals with hyperlipidemia, diabetes, and metabolic syndrome. However, further large and well-conducted randomized controlled trials are needed to provide more convincing evidence.
CARDIOVASCULAR RESEARCH
(2023)
Review
Pharmacology & Pharmacy
Morvarid Noormohammadi, Zeinab Ghorbani, Ulrike Lober, Marjan Mahdavi-Roshan, Theda U. P. Bartolomaeus, Asma Kazemi, Nargeskhatoon Shoaibinobarian, Sofia K. Forslund
Summary: Recent studies have shown that probiotics, prebiotics, and synbiotics can affect the levels of adiponectin and leptin, although these findings are still being debated. This study conducted a systematic review and meta-analysis of randomized controlled trials to explore the impact of probiotic/synbiotic supplementation on appetite-regulating hormones and the desire to eat. The results showed that probiotic/synbiotic supplementation led to a significant decrease in leptin concentration and a slight increase in adiponectin levels, while also slightly increasing the desire to eat.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Genetics & Heredity
Florence Thirion, Finn Sellebjerg, Yong Fan, Liwei Lyu, Tue H. Hansen, Nicolas Pons, Florence Levenez, Benoit Quinquis, Evelina Stankevic, Helle B. Sondergaard, Thomas M. Dantoft, Casper S. Poulsen, Sofia K. Forslund, Henrik Vestergaard, Torben Hansen, Susanne Brix, Annette Oturai, Per Soelberg Sorensen, Stanislav D. Ehrlich, Oluf Pedersen
Summary: This study found significant differences in the gut microbiota of patients with multiple sclerosis, which are directly associated with inflammation markers. In treatment-naive cases, the bacterial richness is positively linked to disease activity. However, two symbiotic bacterial species that produce beneficial immune-modulating compounds were found in non-disease-active cases, providing a rationale for testing these bacteria as adjunct therapeutics in future clinical trials.
Article
Geriatrics & Gerontology
Bastian Kochlik, Kristina Franz, Thorsten Henning, Daniela Weber, Andreas Wernitz, Catrin Herpich, Franziska Jannasch, Volkan Aykac, Ursula Mueller-Werdan, Matthias B. Schulze, Tilman Grune, Kristina Norman
Summary: This study found that specific biomarker patterns, including nutrients, oxidative stress, inflammation biomarkers, and muscle-related biomarkers, are associated with frailty among multi-morbid patients. These biomarker patterns provide more informative results than analyzing single biomarkers.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Review
Immunology
Alex Kayongo, Nicole M. Robertson, Trishul Siddharthan, Moses Levi Ntayi, Josephine Caren Ndawula, Obondo J. Sande, Bernard S. Bagaya, Bruce Kirenga, Harriet Mayanja-Kizza, Moses L. Joloba, Sofia K. Forslund
Summary: Chronic Obstructive Pulmonary Disease (COPD) is a major contributor to global mortality, with three million deaths reported annually. The impact is expected to increase, with approximately five million people predicted to die from COPD-related causes each year in the next 40 years. The immune mechanisms driving disease progression, as well as the relationship between airway microbiome changes and immune activation, remain unclear.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Beatriz F. Corte-Real, Ibrahim Hamad, Rebeca Arroyo Hornero, Sabrina Geisberger, Joris Roels, Lauren Van Zeebroeck, Aleksandra Dyczko, Marike W. van Gisbergen, Henry Kurniawan, Allon Wagner, Nir Yoset, Susanne N. Y. Weiss, Klaus G. Schmetterer, Agnes Schroeder, Luka Krampert, Stefanie Haase, Hendrik Bartolomaeus, Niels Hellings, Yvan Saeys, Ludwig J. Dubois, Dirk Brenner, Stefan Kempa, David A. Hafler, Johannes Stegbauer, Ralf A. Linker, Jonathan Jantsch, Dominik N. Mueller, Markus Kleinewietfeld
Summary: High salt induces metabolic reprogramming in human Tregs, leading to pro-inflammatory features and FOXP3 downregulation, which has important implications for autoimmunity.
Article
Physiology
Anja Maehler, Till Schuette, Jochen Steiniger, Michael Boschmann
Summary: This study presents a methodological overview of a respiration chamber at the Experimental and Clinical Research Center in Berlin, Germany. They have investigated 750 healthy subjects and patients with various diseases since 2010. The chamber is able to measure resting energy expenditure, dietary-induced thermogenesis, and activity energy expenditure.
EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY
(2023)
Article
Urology & Nephrology
Kai -Uwe Eckardt, Cynthia Delgado, Hiddo J. L. Heerspink, Roberto Pecoits-Filho, Ana C. Ricardo, Benedicte Stengel, Marcello Tonelli, Michael Cheung, Michel Jadoul, Wolfgang C. Winkelmayer, Holly Kramer
Summary: Chronic kidney disease affects a large number of people globally, calling for urgent prevention and management. New tools and interventions, such as biomarkers and artificial intelligence techniques, have emerged to improve the quality and precision of care for CKD. The 2022 KDIGO Controversies Conference discussed best practices and identified knowledge gaps in CKD diagnosis, treatment, and care delivery.
KIDNEY INTERNATIONAL
(2023)
Article
Medicine, General & Internal
Joonatan Palmu, Christin S. Boerschel, Alfredo Ortega-Alonso, Lajos Marko, Michael Inouye, Pekka Jousilahti, Rodolfo A. Salido, Karenina Sanders, Caitriona Brennan, Gregory C. Humphrey, Jon G. Sanders, Friederike Gutmann, Dominik Linz, Veikko Salomaa, Aki S. Havulinna, Sofia K. Forslund, Rob Knight, Leo Lahti, Teemu Niiranen, Renate B. Schnabel
Summary: This study found that the gut microbiome composition is associated with the risk of atrial fibrillation (AF). Certain microbial genera were found to be related to the occurrence and prediction of AF. These findings establish the basis for using microbiome profiling in predicting AF risk, but further research is needed before microbiome sequencing can be used in prevention and targeted treatment of AF.
Article
Microbiology
Alex Kayongo, Theda Ulrike Patricia Bartolomaeus, Till Birkner, Lajos Marko, Ulrike Loeber, Edgar Kigozi, Carolyne Atugonza, Richard Munana, Denis Mawanda, Rogers Sekibira, Esther Uwimaana, Patricia Alupo, Robert Kalyesubula, Felix Knauf, Trishul Siddharthan, Bernard S. Bagaya, David P. Kateete, Moses L. Joloba, Nelson K. Sewankambo, Daudi Jjingo, Bruce Kirenga, William Checkley, Sofia K. Forslund
Summary: Even with ART available, HIV-infected individuals are still at high risk of noninfectious lung diseases. Recent studies suggest a link between respiratory microbiota and chronic lung inflammation. This study investigates the microbiome patterns and associations among people living with HIV (PLWH) and COPD in Sub-Saharan Africa. The results reveal significant alterations in the respiratory microbiota among PLWH, indicating the potential of using the sputum microbiome for COPD prognostic and risk stratification strategies.
MICROBIOLOGY SPECTRUM
(2023)
Article
Neurosciences
Florence Thirion, Helene Speyer, Tue Haldor Hansen, Trine Nielsen, Yong Fan, Emmanuelle Le Chatelier, Sebastien Fromentin, Magali Berland, Florian Plaza Onate, Nicolas Pons, Nathalie Galleron, Florence Levenez, Lajos Marko, Till Birkner, Torben Jorgensen, Sofia K. Forslund, Henrik Vestergaard, Torben Hansen, Merete Nordentoft, Ole Mors, Michael E. Benros, Oluf Pedersen, Stanislav D. Ehrlich
Summary: This study found significant differences in the gut microbiota between patients with schizophrenia, healthy individuals, and individuals with metabolic syndrome. The functional potential of the gut microbiota was associated with cognitive function in patients with schizophrenia, suggesting the gut microbiota could be a target for intervention to alleviate cognitive dysfunction.
BIOLOGICAL PSYCHIATRY: GLOBAL OPEN SCIENCE
(2023)