Journal
CARBOHYDRATE POLYMERS
Volume 245, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.116469
Keywords
Ilex latifolia; Polysaccharide; Immunoregulatory activity; Zebrafish
Categories
Funding
- National Key Research and Development Program of China [2018YFA0507204]
- National Natural Science Foundation of China [U1703107, U1801288, 21665013, 21864016]
- Natural Science Foundation of Tianjin [19JCYBJC28100]
- Hundred Young Academic Leaders Program of Nankai University
- State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University) [CMEMR2019-B01]
- Scientific Research Program of the Higher Education Institution of Xinjiang Uygur Autonomous Region [XJEDU20171010]
- Key Laboratory of Xinjiang Uygur Autonomous Region [2015KL030, 2017D04019]
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A novel polysaccharide (ILP50-2) was extracted, isolated and purified from the leaves of Ilex latifolia Thunb. Its structure was characterized as a repeating unit consisting of alpha-L-Araf-(1 ->,-> 3)-alpha-L-Araf-(1 ->,-> 5)-alpha-L-Araf-(1 ->,-> 3,5)-alpha-L-Araf-(1 ->,-> 2)-alpha-L-Rhap-(1 ->,-> 2,4)-alpha-L-Rhap-(1 ->, beta-D-Galp-(1 ->,-> 4)-beta-D-Galp-(1 ->,-> 4)-beta-D-Glcp-(1 ->,-> 6)-alpha-D-Manp-(1 ->, and -> 3,6)-alpha-D-Galp-(1 ->. The absolute molecular weight of ILP50-2 was 1.49 x 10(5) g/mol, which adapted a compact coil conformation in 0.1 M NaCl solution with R-z of 25.4 nm. Furthermore, ILP50-2 exhibited immunoregulatory activity, mainly through enhancing the phagocytosis ability of macrophages and prompting the release of nitric oxide (NO) and cytokines tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and interleukin-1 beta (IL-1 beta). Simultaneously, ILP50-2 was found to significantly increase the release of ROS and NO in zebrafish embryos, showing immunoregulatory effects in vivo.
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