Journal
BMC MICROBIOLOGY
Volume 20, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s12866-020-01742-6
Keywords
Yeasts; Intracellular lipids; Nile red; Volatile fatty acids; Single cell oils
Categories
Funding
- European project [720777]
- national funds through the FCT I.P. [UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569)]
- ERDF through the COMPETE2020
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Background Over the last years oleaginous yeasts have been studied for several energetic, oleochemical, medical and pharmaceutical purposes. However, only a small number of yeasts are known and have been deeply exploited. The search for new isolates with high oleaginous capacity becomes imperative, as well as the use of alternative and ecological carbon sources for yeast growth. Results In the present study a high-throughput screening comprising 366 distinct yeast isolates was performed by applying an optimised protocol based on two approaches: (I) yeast cultivation on solid medium using acetic acid as carbon source, (II) neutral lipid estimation by fluorimetry using the lipophilic dye Nile red. Conclusions Results showed that, with the proposed methodology, the oleaginous potential of yeasts with broad taxonomic diversity and variety of growth characteristics was discriminated. Furthermore, this work clearly demonstrated the association of the oleaginous yeast character to the strain level, contrarily to the species-level linkage, as usually stated.
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