Article
Forestry
Liang-Jing Sheng, Xiao-Qian Weng, Ming-Qing Weng, Ya-Jie Guo, Rebeca Carballar-Lejarazu, Fei-Ping Zhang, Song-Qing Wu
Summary: In this study, a tryptophan 2,3-dioxygenase (TDO) gene related to ommochrome synthesis in insects was identified. Using RNAi technology, the TDO gene of Monochamus alternatus was silenced, resulting in white-eye phenotypes in the pupal and adult stages. The expression of the TDO gene was significantly decreased 48 hours after injection. This discovery is important for future genetic engineering tools to control M. alternatus populations.
Article
Biotechnology & Applied Microbiology
Shaozhen Wang, Hong Han, Yajie Guo, Rebeca Carballar-Lejarazu, Liangjing Sheng, Guanghong Liang, Xia Hu, Rong Wang, Feiping Zhang, Songqing Wu
Summary: The study isolated the Pseudomonas entomophila strain CSHH2 from nematode-infected Pinus massoniana, which showed significant insecticidal activity against Monochamus alternatus larvae and was sensitive to most drugs tested in antimicrobial sensitivity assay.
BIOCONTROL SCIENCE AND TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Yu-Ping Zha, Xiao-Ling Wu, Zi-Yi Zhang, Jing-Yuan Chen, Qi-Cai Chen
Summary: This study investigated the effects of ultrasound on the juvenile hormone levels in Monochamus alternatus adults, a major global pest. Results showed that different frequencies of ultrasound exposure had varying effects on the JHIII titers of beetles at different ages.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Dong-Zhen Li, Shuang-Gang Duan, Rui-Nan Yang, Shan-Cheng Yi, Ao Liu, Hazem Elewa Abdelnabby, Man-Qun Wang
Summary: This paper reveals the gradient expression pattern of insect odorant-binding protein Obp19 in the antennae of the Japanese pine sawyer, as well as the direct regulatory relationship with the transcription factor BarH1, which is essential for understanding the biological functions of OBPs and olfactory mediated behaviors.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Forestry
Kexin Huang, Jian Xiang, Yuying Ma, Jinping Cheng, Jie Gu, Meng Hu, Yuan Yang, Yanming Fang, Genmei Wang, Huanchao Zhang
Summary: This study examined the response of soil microbial communities to elevation and their driving factors in two different pine forests using high-throughput sequencing technology. The results showed that elevation significantly affected soil organic carbon, total nitrogen, and microbial diversity. Soil bacteria were found to be more sensitive to elevation than soil fungi. Overall, elevation and soil characteristics (SOC and TN) were identified as the primary drivers of soil microbial diversity and community composition in the two pine forests.
Article
Environmental Sciences
Yafei Shen, Lei Lei, Wenfa Xiao, Ruimei Cheng, Changfu Liu, Xiaoyu Liu, Hu Lin, Lixiong Zeng
Summary: Soil microbial residues in Pinus massoniana plantations of different ages and depths were characterized using amino sugar biomarkers, revealing variations in their contributions. The age of the plantation and soil depth were found to influence the microbial residue patterns differently.
ENVIRONMENTAL RESEARCH
(2023)
Article
Microbiology
Xin Hao, Xuefeng Liu, Jie Chen, Bowen Wang, Yang Li, Yi Ye, Wei Ma, Ling Ma
Summary: This article investigated the differences in the endophytic microbial community between Pinus massoniana trees infected with pine wood nematode (PWN) and healthy trees. The study found that infected trees had increased abundance of pathogenic fungi and specific fungi species, which were closely related to the vulnerability of the trees. In healthy trees, the dominant functional guilds were parasites, plant pathogens, and saprotrophs. The study also revealed that the decrease in the trees' immunity and the changes in resin secretion were associated with the development of pine wilt disease.
Article
Biotechnology & Applied Microbiology
Peng Ye, Ye Liu, Ye-Jun Cai, Hong Yang, Hong-Tao Xu, Zhi-Yue Lu
Summary: Tongue squamous cell carcinoma patients showed a significant increase in oral microbiotic diversity, with differences in bacterial community structure and decreased levels of specific taxa in cancer samples. The bacterial community in cancer samples appeared more conserved than in normal tissue, suggesting potential carcinogenic mechanisms.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Daokun Xiang, Hazem Abdelnabby, Man-Qun Wang
Summary: This study discovered that MaltOBP12 has binding affinities to 9 pine volatiles and revealed the structure and protein-ligand interaction mechanism through modeling and molecular docking. These findings not only help us understand how odorant-binding proteins flexibly bind odorants, but also promote the screening of behaviorally active compounds through computer methods to prevent M. alternatus.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Binbin Li, Shuji Liu, Xiaoting Chen, Yongchang Su, Nan Pan, Dengyuan Liao, Kun Qiao, Yihui Chen, Zhiyu Liu
Summary: This study used 16S rDNA high-throughput sequencing technology to investigate the quality changes, dynamic changes in microbial composition, and diversity changes in large yellow croaker during 4 degrees C refrigeration. The results showed that the total viable count, total volatile basic nitrogen, and thiobarbituric acid-reactive substances levels increased over time. The species richness and diversity also declined as the storage time increased. Pseudomonas was identified as the dominant spoilage bacteria, and three strains of Pseudomonas were ranked for their spoilage-causing ability.
Article
Forestry
Jing Huang, Yajie Guo, Xiaoqian Weng, Yunzhu Sun, Rebeca Carballar-Lejarazu, Xia Hu, Rong Wang, Guanghong Liang, Feiping Zhang, Songqing Wu
Summary: The study used next generation sequencing technology to obtain a full transcriptome from the four larval instars of Monochamus alternatus and successfully constructed a transcriptome database. A large number of genes related to lignocellulose, putative Bt receptors, and digestion were identified. The research provides valuable information for the development of new molecular tools for managing Monochamus alternatus.
Article
Microbiology
Yao Ling, Wen-Jing Li, Fei-Fei Li, Xiao-Bao Xue, Yuan-Yi Gao, Li Wang, Ke Liang, Xin-Jiang Li
Summary: This study describes the gut microbiota diversity of four species of grasshoppers and finds that the gut microbiota is correlated with the cellulose and hemicellulose digestibility of grasshoppers. This is important for developing grasshopper digestive tracts as bioreactors for cellulose decomposition, improving the utilization of agricultural straw, and producing clean biomass energy.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Eleni Kamilari, Marina Efthymiou, Dimitrios A. Anagnostopoulos, Dimitrios Tsaltas
Summary: The research utilized high-throughput sequencing to analyze the bacterial diversity of traditional Cyprus sausages from the Troodos mountainous region of Pitsilia and industrially produced sausages. The findings showed that Pitsilia sausages had significantly higher bacterial diversity, suggesting that microbial communities could serve as an additional tool for identifying the authenticity of Cypriot sausages.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Energy & Fuels
Taotao Zhang, Yahui Chen, Yongyun Li, Peng Chen, Haitao Ma, Pengju Han, Can Wang, Wei Liu, Yao Wang, Renwei Qing, Fei Xu
Summary: Conventionally, microbial fuel cells (MFCs) are classified into dual-chamber MFCs and single-chamber MFCs. However, their complex configuration and high resistance limit their practical applications. In this study, we constructed three MFCs using different anode materials and found that the Ag-Ni/Ti-MFC performed best in bioelectricity output and removal of target pollutants.
Article
Forestry
Yusuke Nakayama, Katsumi Togashi
Summary: Monochamus alternatus and M. saltuarius transmit Bursaphelenchus xylophilus causing pine wilt disease through oviposition wounds. The size of oviposition sites and the positioning of eggs were found to be related to the number of eggs deposited. Bark samples of specific diameters were suitable for inspecting the nematodes transmitted by each species.
JOURNAL OF FOREST RESEARCH
(2023)
Article
Agronomy
Yajie Guo, Yafang Wang, Anthony J. O'Donoghue, Zhenze Jiang, Rebeca Carballar-Lejarazu, Guanghong Liang, Xia Hu, Rong Wang, Lei Xu, Xiong Guan, Feiping Zhang, Songqing Wu
PEST MANAGEMENT SCIENCE
(2020)
Article
Entomology
Yajie Guo, Rebeca Carballar-Lejarazu, Liangjing Sheng, Yan Fang, Shaozhen Wang, Guanghong Liang, Xia Hu, Rong Wang, Feiping Zhang, Songqing Wu
JOURNAL OF ECONOMIC ENTOMOLOGY
(2020)
Article
Microbiology
Qiliao Liao, Yajie Guo, Jianshuang Zhou, Yi Wan, Rebeca Carballar-Lejarazu, Liangjing Sheng, Feiping Zhang, Songqing Wu, Shuangquan Zou
CURRENT MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Shaozhen Wang, Hong Han, Yajie Guo, Rebeca Carballar-Lejarazu, Liangjing Sheng, Guanghong Liang, Xia Hu, Rong Wang, Feiping Zhang, Songqing Wu
Summary: The study isolated the Pseudomonas entomophila strain CSHH2 from nematode-infected Pinus massoniana, which showed significant insecticidal activity against Monochamus alternatus larvae and was sensitive to most drugs tested in antimicrobial sensitivity assay.
BIOCONTROL SCIENCE AND TECHNOLOGY
(2022)
Article
Entomology
Yajie Guo, Yunzhu Sun, Qiliao Liao, Rebeca Carballar-Lejarazu, Liangjing Sheng, Shaozhen Wang, Jianshuang Zhou, Feiping Zhang, Songqing Wu
Summary: This study investigates the proteolytic activation process of Cry3Aa in the midgut of RPW larvae, revealing that Cry3Aa toxin is weakly toxic to RPW and can be over-digested by gut proteases under certain conditions. The gut proteases of RPW larvae were found to mainly consist of serine proteases.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2021)
Article
Forestry
Jing Huang, Yajie Guo, Xiaoqian Weng, Yunzhu Sun, Rebeca Carballar-Lejarazu, Xia Hu, Rong Wang, Guanghong Liang, Feiping Zhang, Songqing Wu
Summary: The study used next generation sequencing technology to obtain a full transcriptome from the four larval instars of Monochamus alternatus and successfully constructed a transcriptome database. A large number of genes related to lignocellulose, putative Bt receptors, and digestion were identified. The research provides valuable information for the development of new molecular tools for managing Monochamus alternatus.
Article
Zoology
Yajie Guo, Mingqing Weng, Yunzhu Sun, Rebeca Carballar-Lejarazu, Songqing Wu, Chunlan Lian
Summary: The pine wilt disease caused by Bursaphelenchus xylophilus results in significant ecological and economic losses. This study evaluated the nematocidal activity of various toxins produced by Bacillus thuringiensis against B. xylophilus. Six toxins were identified to have high toxicity, leading to the shrinkage, thinning, and degeneration of the pinewood nematodes. These findings provide valuable insights into the action mechanism of nematocidal toxins and their potential use in the biological control of B. xylophilus.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2022)
Article
Agronomy
Yajie Guo, Jiayi Ma, Yunzhu Sun, Rebeca Carballar-Lejarazu, Mingqing Weng, Wenchao Shi, Jieqin Wu, Xia Hu, Rong Wang, Feiping Zhang, Songqing Wu
Summary: Pine wilt disease (PWD) caused by pinewood nematodes is a destructive disease of pine trees. This study investigated the spatiotemporal transport pattern and residue behavior of fluopyram, a prospective trunk-injection agent against pinewood nematodes. Fluopyram was found to be evenly distributed in tree tissues and exerted substantial control over PWD, with a persistence of up to 3 years.
PEST MANAGEMENT SCIENCE
(2023)
Article
Forestry
Yajie Guo, Jiayi Ma, Ziyi You, Mingqing Weng, Rebeca Carballar-Lejarazu, Wenlong Jiao, Jieqin Wu, Xia Hu, Rong Wang, Feiping Zhang, Songqing Wu
Summary: Fluopyram, a novel nematicide compound, has been found to effectively inhibit pine wilt disease and prevent its occurrence in healthy pine seedlings. When applied in the field, Fluopyram can be absorbed and distributed throughout the pine tree, with a stable residual concentration at 30 days post-application. The formulation sprayed by agricultural unmanned aerial vehicles achieved a control efficiency of 90.48% in the forest. The half-life of Fluopyram was 346.6 days, indicating its persistence for 3 years.
Article
Biotechnology & Applied Microbiology
Xiaohua Hu, Yajie Guo, Songqing Wu, Zhaoxia Liu, Tao Fu, Ensi Shao, Carballar-Lejarazu Rebeca, Guohui Zhao, Zhipeng Huang, Ivan Gelbic, Xiong Guan, Shuangquan Zou, Lei Xu, Lingling Zhang
BIOCONTROL SCIENCE AND TECHNOLOGY
(2017)