Article
Biochemistry & Molecular Biology
Chih-Shiang Chang, Vathan Kumar, Der-Yen Lee, Yeh Chen, Yu-Chieh Wu, Jing-Yan Gao, Po-Chen Chu
Summary: These benzo-fused derivatives may exert antitumor activities by targeting Hsp70 with differential antiproliferative efficacy in breast cancer cells but not in nontumorigenic breast epithelial cells.
CURRENT MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Ziqian Wang, Ting Song, Zongwei Guo, Laura B. Uwituze, Yafei Guo, Hong Zhang, Hang Wang, Xiaodong Zhang, Hao Pan, Tong Ji, Fangkui Yin, Sheng Zhou, Jian Dai, Zhichao Zhang
Summary: The novel Hsp70 inhibitor S1g-6 disrupts Hsp70-Bim protein-protein interactions and selectively antagonizes Hsp70 ATPase activity, leading to selective apoptosis induction in certain cancer cell lines. This represents a new class of antitumor candidates targeting cancer-related Hsp70-Bim interactions.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Materials Science, Biomaterials
Dan Peng, Genhua Liu, Ye He, Pengfei Gao, Shuangquan Gou, Jing Wu, Jinxiu Yu, Peng Liu, Kaiyong Cai
Summary: This study successfully developed novel nanoparticles to effectively eliminate bacterial biofilms, enhancing photothermal therapy efficacy at low temperatures.
BIOMATERIALS SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Siddhi Omkar, Tasaduq H. Wani, Bo Zheng, Megan M. Mitchem, Andrew W. Truman
Summary: This study shows that APE2 and Apn2 proteins depend on the chaperone system in yeast and mammalian cells, and suggests that inhibiting chaperones may be a potential anticancer therapy targeting APE2-mediated processes.
Review
Cell Biology
Sihan Du, Ying Liu, Yuan Yuan, Yuran Wang, Yanfang Chen, Shuai Wang, Yuhua Chi
Summary: HSP70 is a highly conserved protein that plays a crucial role in maintaining protein homeostasis. It has been found that HSP70 is abundantly expressed in cancer and contributes to tumor resistance to radiotherapy through various mechanisms. Inhibiting HSP70 can enhance the effectiveness of radiotherapy and serve as an effective target for cancer treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
S. Majhi, V. C. Peddiraju, A. Mishra
Summary: Bacterial colonization and biofilm formation on surfaces lead to worldwide infections, but research has shown that immobilizing a designed peptide, KLR, onto stainless steel surfaces can significantly reduce bacterial colonization and be non-toxic to mammalian cells.
MATERIALS TODAY CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Eduardo Pinho Melo, Tasuku Konno, Ilaria Farace, Mosab Ali Awadelkareem, Lise R. Skov, Fernando Teodoro, Teresa P. Sancho, Adrienne W. Paton, James C. Paton, Matthew Fares, Pedro M. R. Paulo, Xin Zhang, Edward Avezov
Summary: This study reveals that the endoplasmic reticulum (ER) has the capability to resolve protein aggregates and shows that the stress-responsive ER molecular chaperone - BiP plays a catalytic role in this process.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Shinya Sugimoto, Kunitoshi Yamanaka, Tatsuya Niwa, Yurika Terasawa, Yuki Kinjo, Yoshimitsu Mizunoe, Teru Ogura
Summary: This study demonstrated the role of JDPs in bacterial survival during heat stress and the formation of curli-extracellular amyloid fibers. Genetic analysis revealed the essential role of DnaJ in survival at high temperature and the ability of either DnaJ or CbpA to activate curli production. Biochemical analyses showed that DnaJ and CbpA are involved in regulating the master regulator CsgD and maintaining CsgA in a translocation-competent state.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Chemistry, Medicinal
Yunpeng Yi, Jiaming Zhang, Jiakun Zuo, Maolu Zhang, Shifa Yang, Zhongli Huang, Guiyu Li, Ruofeng Shang, Shuqian Lin
Summary: A series of pyridinium cation-substituted pleuromutilin analogues were designed, synthesized and evaluated, and e4 showed the highest antibacterial activity against multi-drug resistant bacteria. It not only inhibited bacterial protein synthesis but also disrupted bacterial cell walls. In vivo, e4 exhibited an ED50 of 5.68 mg/kg against multi-drug resistant Staphylococcus aureus and showed low toxic effects with an LD50 of 879 mg/kg in toxicity tests. These findings suggest that e4 could be considered as a new therapeutic candidate for bacterial infections.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Eugenia M. Clerico, Alexandra K. Pozhidaeva, Rachel M. Jansen, Can Ozden, Joseph M. Tilitsky, Lila M. Gierasch
Summary: This research investigated how Hsp70 binds to full-length client proteins by analyzing complex structures and experiments, revealing that many sequences can choose from multiple binding modes and binding preference depends on the compatibility of substrate residue side chains with binding pockets. The study also found that multiple chaperones can simultaneously bind to multiple sites on the protein substrate, and these sites behave independently.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Chia-Hung Tsai, Jing-Ru Weng, Hsiang-Wen Lin, Meng-Tien Lu, Yu-Chi Liu, Po-Chen Chu
Summary: The study found that Hsp70 inhibitors can suppress the expansion of BCSCs in TNBC cells by regulating beta-catenin. This finding provides a potential target for the development of new strategies for TNBC therapy.
Review
Genetics & Heredity
Lu Zhao, Ting Jia, Qingsong Jiao, Xueyun Hu
Summary: J-proteins, a type of molecular chaperone, play important roles in chloroplast development, photosynthesis, seed germination, and stress response in plants.
Article
Cell Biology
Kang Kang, Xiaoyun Liao, Qing Li, Jidong Chen, Yanqin Niu, Yan Zeng, Sijian Xia, Le Zeng, Shide Liu, Deming Gou
Summary: This study revealed that downregulation of miR-23a-5p in response to high NaCl treatment facilitates cellular adaptation to hypertonic stress in mouse inner medullary collecting duct cells through modulating HSPA1B.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Athar Alam, Jeanette E. Broms, Rajender Kumar, Anders Sjostedt
Summary: Bacterial survival within a mammalian host relies on sensing environmental perturbations and initiating appropriate counter-responses, where bacterial chaperone systems, particularly ClpB, play indispensable roles. ClpB is crucial for bacterial survival under various stress conditions, especially heat shock, and also regulates secretion of bacterial effector molecules related to type VI secretion systems.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Cell Biology
Graham Chakafana, Addmore Shonhai
Summary: The article discusses the role and unique structural features of non-canonical Hsp70s in cancer development, highlighting their distinction from canonical Hsp70s and their potential in cancer therapy.
Article
Chemistry, Physical
Angela E. Varela, Jonathan F. Lang, Yufan Wu, Matthew D. Dalphin, Andrew J. Stangl, Yusuke Okuno, Silvia Cavagnero
JOURNAL OF PHYSICAL CHEMISTRY B
(2018)
Article
Chemistry, Physical
Hanming Yang, Heike Hofstetter, Silvia Cavagnero
JOURNAL OF CHEMICAL PHYSICS
(2019)
Article
Chemistry, Analytical
Jongchan Lee, Sho Hee Park, Silvia Cavagnero, Jung Ho Lee
ANALYTICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Susanna K. Yaeger-Weiss, Theodore S. Jennaro, Miranda Mecha, Jenna H. Becker, Hanming Yang, Gordon L. W. Winkler, Silvia Cavagnero
Article
Chemistry, Physical
Rayna M. Addabbo, Matthew D. Dalphin, Miranda F. Mecha, Yue Liu, Alexios Staikos, Valeria Guzman-Luna, Silvia Cavagnero
JOURNAL OF PHYSICAL CHEMISTRY B
(2020)
Article
Biochemical Research Methods
Hanming Yang, Miranda F. Mecha, Collin P. Goebel, Silvia Cavagnero
Summary: Low-concentration photochemically induced dynamic nuclear polarization (LC-photo-CIDNP) is an effective tool for hyperpolarizing aromatic amino acids in solution, but long-term optical irradiation may degrade the target molecules and photosensitizers. The use of reductive radical quenchers such as vitamin C or 2-mercaptoethylamine can extend the data acquisition time and increase the sensitivity of LC-photo-CIDNP by more than 100%, without causing spectral distortions. This approach reduces the extent of photodamage by reducing transient free radicals, allowing for long-term high-resolution data collection.
JOURNAL OF MAGNETIC RESONANCE
(2021)
Article
Chemistry, Physical
Rachel B. Hutchinson, Xi Chen, Ningkun Zhou, Silvia Cavagnero
Summary: The combination of fluorescence anisotropy decay with microscale-volume viscometry allows for determining the size of compact regions in nascent proteins, shedding light on post-translational folding processes in cells.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Biology
Valeria Guzman-Luna, Andrew M. Fuchs, Anna J. Allen, Alexios Staikos, Silvia Cavagnero
Summary: Guzman-Luna et al. present a study on the interaction between unstructured nascent chains and specific ribosomal proteins near the nascent polypeptide exit tunnel, showing that these interactions are dependent on the peptide length, surface charge, and Mg+2 concentration, providing insights into co-translational protein folding and misfolding/aggregation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Hanming Yang, Siyu Li, Clayton A. Mickles, Valeria Guzman-Luna, Kenji Sugisaki, Clayton M. Thompson, Hung H. Dang, Silvia Cavagnero
Summary: NMR spectroscopy is a powerful tool for studying molecular structure and dynamics. This study demonstrates the use of low-concentration photochemically induced dynamic nuclear polarization (LC-photo-CIDNP) to enhance NMR sensitivity and enable rapid detection of molecules at extremely low concentrations.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Chemistry, Multidisciplinary
James Eills, Dmitry Budker, Silvia Cavagnero, Eduard Y. Chekmenev, Stuart J. Elliott, Sami Jannin, Anne Lesage, Joerg Matysik, Thomas Meersmann, Thomas Prisner, Jeffrey A. Reimer, Hanming Yang, Igor V. Koptyug
Summary: Magnetic resonance techniques, especially medical magnetic resonance imaging, are widely used in various scientific disciplines and practical applications. The development of spin hyperpolarization has greatly boosted both fundamental and applied magnetic resonance. Hyperpolarization techniques significantly enhance the signal intensity of magnetic resonance, overcoming its low sensitivity and opening up new possibilities for applications.
Article
Chemistry, Physical
Rayna M. Addabbo, Rachel B. Hutchinson, Heather J. Allaman, Matthew D. Dalphin, Miranda F. Mecha, Yue Liu, Alexios Staikos, Silvia Cavagnero
Summary: Proteins released from the ribosome are prone to aggregation, and understanding the role of chaperones during this process is important. The Hsp70 and trigger factor chaperone systems interact with nascent proteins, but it is unclear if they can prevent aggregate formation. This study shows that the Hsp70 chaperone system has limitations in preventing soluble-aggregate formation for highly aggregation-prone proteins.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Chemistry, Organic
Clayton M. Thompson, Allwin D. McDonald, Hanming Yang, Silvia Cavagnero, Andrew R. Buller
ORGANIC & BIOMOLECULAR CHEMISTRY
(2020)