Spatiotemporal Control of CRISPR/Cas9 Function in Cells and Zebrafish using Light‐Activated Guide RNA

Precision control of CRISPR-Cas9 using small molecules and light

(2019) Soumyashree Ashok Gangopadhyay et al.   BIOCHEMISTRY

Near-infrared upconversion–activated CRISPR-Cas9 system: A remote-controlled gene editing platform

(2019) Yongchun Pan et al.   Science Advances

Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs

(2019) Kale Kundert et al.   Nature Communications

The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation

(2019) Qian Zhang et al.   Science Advances

Optochemical Control of Biological Processes in Cells and Animals

(2018) Nicholas Ankenbruck et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

CRISPR base editors: genome editing without double-stranded breaks

(2018) Ayman Eid et al.   BIOCHEMICAL JOURNAL

Optogenetics: A Primer for Chemists

(2018) Colin P. O'Banion et al.   CHEMBIOCHEM

Synthetic far-red light-mediated CRISPR-dCas9 device for inducing functional neuronal differentiation

(2018) Jiawei Shao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering the Delivery System for CRISPR-Based Genome Editing

(2018) Zachary Glass et al.   TRENDS IN BIOTECHNOLOGY

The CRISPR tool kit for genome editing and beyond

(2018) Mazhar Adli   Nature Communications

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities

(2018) Kendall R. Sanson et al.   Nature Communications

Genome editing via delivery of Cas9 ribonucleoprotein

(2017) Mark A. DeWitt et al.   METHODS

Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes

(2017) Brett T Staahl et al.   NATURE BIOTECHNOLOGY

Illuminating developmental biology through photochemistry

(2017) Lukasz Kowalik et al.   Nature Chemical Biology

Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics

(2017) John C Rose et al.   NATURE METHODS

Improving CRISPR–Cas specificity with chemical modifications in single-guide RNAs

(2017) Daniel E Ryan et al.   NUCLEIC ACIDS RESEARCH

Rational design of inducible CRISPR guide RNAs for de novo assembly of transcriptional programs

(2017) Quentin R. V. Ferry et al.   Nature Communications

Chemical and Biophysical Modulation of Cas9 for Tunable Genome Engineering

(2016) James K. Nuñez et al.   ACS Chemical Biology

Conditional Control of CRISPR/Cas9 Function

(2016) Wenyuan Zhou et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Design of Switchable Chimeric Antigen Receptor T Cells Targeting Breast Cancer

(2016) Yu Cao et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors

(2016) Piyush K. Jain et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

CRISPR/Cas9 in Genome Editing and Beyond

(2016) Haifeng Wang et al.   Annual Review of Biochemistry

Improved delivery of Cas9 protein/gRNA complexes using lipofectamine CRISPRMAX

(2016) Xin Yu et al.   BIOTECHNOLOGY LETTERS

Biology and Applications of CRISPR Systems: Harnessing Nature’s Toolbox for Genome Engineering

(2016) Addison V. Wright et al.   CELL

Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes

(2016) Alexa Burger et al.   DEVELOPMENT

Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch

(2016) Benjamin L Oakes et al.   NATURE BIOTECHNOLOGY

Applications of CRISPR technologies in research and beyond

(2016) Rodolphe Barrangou et al.   NATURE BIOTECHNOLOGY

Multidimensional chemical control of CRISPR–Cas9

(2016) Basudeb Maji et al.   Nature Chemical Biology

A chemical-inducible CRISPR–Cas9 system for rapid control of genome editing

(2016) Kaiwen Ivy Liu et al.   Nature Chemical Biology

Complex transcriptional modulation with orthogonal and inducible dCas9 regulators

(2016) Yuchen Gao et al.   NATURE METHODS

Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae

(2016) Ryo Higuchi-Sanabria et al.   PLoS One

Optical Control of CRISPR/Cas9 Gene Editing

(2015) James Hemphill et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

(2015) Silvana Konermann et al.   NATURE

Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

(2015) Ayal Hendel et al.   NATURE BIOTECHNOLOGY

A split-Cas9 architecture for inducible genome editing and transcription modulation

(2015) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers

(2015) Isaac B Hilton et al.   NATURE BIOTECHNOLOGY

Photoactivatable CRISPR-Cas9 for optogenetic genome editing

(2015) Yuta Nihongaki et al.   NATURE BIOTECHNOLOGY

Small molecule–triggered Cas9 protein with improved genome-editing specificity

(2015) Kevin M Davis et al.   Nature Chemical Biology

A light-inducible CRISPR-Cas9 system for control of endogenous gene activation

(2015) Lauren R Polstein et al.   Nature Chemical Biology

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

(2014) Luke A. Gilbert et al.   CELL

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals

(2014) James Hemphill et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Easy quantitative assessment of genome editing by sequence trace decomposition

(2014) Eva K. Brinkman et al.   NUCLEIC ACIDS RESEARCH

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

(2014) Steven Lin et al.   eLife

Optochemical Control of Deoxyoligonucleotide Function via a Nucleobase-Caging Approach

(2013) Qingyang Liu et al.   ACCOUNTS OF CHEMICAL RESEARCH

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System

(2013) Baohui Chen et al.   CELL

A computational platform for robotized fluorescence microscopy (I): High-content image-based cell-cycle analysis

(2013) Laura Furia et al.   CYTOMETRY PART A

Optochemical control of RNA interference in mammalian cells

(2013) Jeane M. Govan et al.   NUCLEIC ACIDS RESEARCH

Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system

(2013) Li-En Jao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA-programmed genome editing in human cells

(2013) Martin Jinek et al.   eLife

Spatiotemporal control of microRNA function using light-activated antagomirs

(2012) Colleen M. Connelly et al.   Molecular BioSystems

Ubiquitous transgene expression and Cre-based recombination driven by the ubiquitin promoter in zebrafish

(2010) C. Mosimann et al.   DEVELOPMENT

Gene Silencing in Mammalian Cells with Light-Activated Antisense Agents

(2008) Douglas D. Young et al.   CHEMBIOCHEM

The IRG mouse: A two-color fluorescent reporter for assessing Cre-mediated recombination and imaging complex cellular relationships in situ

(2008) Rita De Gasperi et al.   GENESIS

Light-triggered polymerase chain reaction

(2007) Douglas D. Young et al.   CHEMICAL COMMUNICATIONS