Article
Veterinary Sciences
Chihai Ji, Ling Zhou, Yonghui Chen, Xueen Fang, Yanhong Liu, Mengkan Du, Xiandong Lu, Qianniu Li, Heng Wang, Yuan Sun, Tian Lan, Jingyun Ma
Summary: This study developed a rapid, portable, sensitive, high-throughput, and accurate microfluidic-LAMP chip detection system for simultaneous detection and differentiation of multiple swine pathogens. The system showed high sensitivity, specificity, and stability, and effectively diagnosed clinical samples.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Materials Science, Multidisciplinary
Nazente Atceken, Muhammad Munzer Alseed, Sajjad Rahmani Dabbagh, Ali K. Yetisen, Savas Tasoglu
Summary: LAMP technology is a fast, efficient, and low-cost diagnostic method for pathogen detection, which can be applied to various bacteria, viruses, and parasitic pathogens. In addition, LAMP technology can also be used for SNP genotyping and mutation detection.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Chemistry, Analytical
Gaihua Cao, Yue Qiu, Keyi Long, Yifan Xiong, MeimeiShi, JunYang, Yingguo Li, Fuping Nie, Danqun Huo, Changjun Hou
Summary: In this study, a fluorescence biosensor based on carbon nanodots and loop-mediated isothermal amplification was established for highly sensitive detection of African swine fever virus (ASFV). The biosensor showed rapid, sensitive, low-cost, and simple detection of ASFV, with high accuracy in actual sample assays.
Article
Microbiology
Weiwei Jiang, Dongying Hu, Yanyan Xu, Yang Chen, Xiaoyang Zhu, Zhao Han, Xin Ye, Xiaojing Li
Summary: In this study, LAMP primers for Trichophyton were designed and a LAMP-Microfluidic chip detection system was developed. The system exhibited high specificity and sensitivity, enabling the detection of six common species of Trichophyton without cross-reactivity with other dermatophytes. The system has the potential for clinical application, offering a simple and efficient way to detect Trichophyton infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Fisheries
Keshun Hu, Ye Li, Feng Wang, Jianying Liu, Yuanyuan Li, Qian Zhao, Xiaoye Zheng, Ningyu Zhu, Xiaoping Yu, Shaohua Fang, Jun Huang
Summary: In this study, a microfluidic chip detection system based on loop-mediated isothermal amplification (LAMP) was developed for the simultaneous detection of DIV1, WSSV, and EHP, which pose serious threats to shrimp farming. The system exhibited high sensitivity, specificity, and rapid detection, making it suitable for field and routine diagnoses in aquaculture.
JOURNAL OF FISH DISEASES
(2023)
Article
Microbiology
Xiaofang Xu, Yiguo Jia, Ruolin Li, Yuting Wen, Yuchen Liang, Guangjie Lao, Xiaojuan Liu, Wei Zhou, Huawei Liu, Jiang Xie, Xiaoxia Wang, Wenming Xu, Qun Sun
Summary: The LAMP-microfluidic chip developed in this study can simultaneously, efficiently, and accurately detect multiple main pathogens in clinical samples of female RTI, providing a valuable reference for screening and early diagnosis during pregnancy.
Review
Virology
Melek Chaouch
Summary: The Covid-19 pandemic has caused significant loss worldwide. The LAMP test is considered an effective diagnostic tool for Covid-19, especially in endemic areas and low-income countries.
REVIEWS IN MEDICAL VIROLOGY
(2021)
Article
Biochemical Research Methods
Chunmei Xie, Shan Chen, Likun Zhang, Xiangpeng He, Yi Ma, Haiping Wu, Bingjie Zou, Guohua Zhou
Summary: A self-driven microfluidic chip was designed for multiplex detection of HBV, HCV, and HIV, achieving quick and efficient detection of target nucleic acids, especially suitable for resource-limited areas.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Gaolian Xu, Julien Reboud, Yunfei Guo, Hao Yang, Hongchen Gu, Chunhai Fan, Xiaohua Qian, Jonathan M. Cooper
Summary: Accelerating the design of nucleic acid amplification methods is challenging, but necessary for diagnosing diseases. This study presents a generalizable isothermal nucleic acid amplification methodology that can be applied to clinical settings, allowing for the detection of short miRNAs and difficult pathogenic targets.
NATURE COMMUNICATIONS
(2022)
Article
Health Care Sciences & Services
Hongling Ou, Yan Wang, Qiyuan Wang, Yingying Ma, Chen Liu, Lianling Jia, Qiaoyun Zhang, Mengwen Li, Xinxin Feng, Meng Li, Xinru Wang, Chengbin Wang
Summary: A rapid detection system combining LAMP technology and microfluidic chip technology was established for multiple pathogens, showing high sensitivity, accuracy, and good performance in clinical sample analysis.
ANNALS OF PALLIATIVE MEDICINE
(2021)
Review
Chemistry, Analytical
Bin Xiao, Ruiming Zhao, Nan Wang, Juan Zhang, Xiaoyun Sun, Ailiang Chen
Summary: Loop-mediated isothermal amplification (LAMP) is a widely used thermostatic nucleic acid amplification technology in clinical diagnosis and food safety detection. Centrifugal microfluidic chips, developed based on traditional microfluidic chips, utilize centrifugal force for liquid flow, mixing, and reaction, eliminating the need for complex valve and pump design. The combination of LAMP and centrifugal microfluidic chips holds great potential for rapid on-site nucleic acid detection.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Article
Medical Laboratory Technology
Enqi Zhang, Hongling Ou, Lianling Jia, Wang Zhang, Yemei Wang, Xinru Wang
Summary: A novel detection method for infectious pathogens based on a microfluidic chip was established in this study, which can quickly and sensitively detect clinically infected humoral samples without relying on expensive equipment or technical personnel.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Microbiology
Yu-Xin Chen, Yi-Rong Lou, Li-Jun Duan, Qian-Jin Zhou, Zhong-Jie Xu, Fang-Jie Chen, Hong-Xian Chen, Gui-Zong Xu, Ai-Fang Du, Jiong Chen
Summary: The combination of a microfluidic chip and loop-mediated isothermal amplification enables the detection of five zoonotic parasites in a fast and accurate manner. This method has the advantages of low reagent consumption, easy operation, and simultaneous analysis of multiple samples and genetic targets, making it suitable for on-site detection and routine monitoring.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Environmental Sciences
Qiqi Xue, Lingwei Sheng, Junjie Guo, Minghui Zou, Qiao Teng, Sijia Xu, Yuanyuan Li, Jinhong Zhao
Summary: In this study, a loop-mediated isothermal amplification (LAMP) method was successfully established for the detection of the Dermatophagoides farinae allergen. This method showed higher sensitivity, specificity, and efficiency compared to conventional PCR. It did not cross-react with other arthropod species commonly found in indoor dust. This study provides a potential model for the rapid detection of allergens produced by other house dust mites in the future.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)