Article
Immunology
Rahel Nardos, Eric T. Leung, Erin M. Dahl, Sean Davin, Mark Asquith, W. Thomas Gregory, Lisa Karstens
Summary: This study reveals the overlap between urogenital microbiomes in the bladder and vagina, with higher overlap in cases. Differences in the central bacteria of the overall community structure were also identified.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Tanya Kumar, MacKenzie Bryant, Kalen Cantrell, Se Jin Song, Daniel McDonald, Helena M. M. Tubb, Sawyer Farmer, Emily S. S. Lukacz, Linda Brubaker, Rob Knight
Summary: The urogenital microbiome is an important yet understudied human microbiome niche, and urine has been found to contain microorganisms that can affect health. It is crucial to study urine sample storage conditions for scientific rigor and reproducibility.
Article
Immunology
Nazema Y. Siddiqui, Li Ma, Linda Brubaker, Jialiang Mao, Carter Hoffman, Erin M. Dahl, Zhuoqun Wang, Lisa Karstens
Summary: The re-analysis of an existing dataset using updated bioinformatic and statistical techniques revealed differences in the identified taxa and associations between bacterial communities and mixed urinary incontinence (MUI). While overall microbial compositions were not associated with MUI, certain bacterial communities were confirmed to be associated with MUI when accounting for covariates.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Immunology
Nadia S. S. Deen, Akash Ahmed, Nazifa Tabassum Tasnim, Nabila Khan
Summary: Expanded quantitative urine culture (EQUC) is an enhanced culture protocol that can detect a wide range of bacteria and fungi in urine specimens. It has the potential to identify emerging pathogens and commensal microbiota that may be missed by standard urinary culture.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Medicine, General & Internal
Dong Soo Kim, Jeong Woo Lee
Summary: Urinary tract infection is a common bacterial infection that can have significant consequences for individuals and society as a whole. Advances in sequencing and culture techniques have revealed a dynamic urinary tract microbiome, challenging the previously held belief that the urinary tract is sterile. Research has identified the normal core microbiota and explored changes in microbial communities due to factors such as sex and age. The pathogenesis of recurrent urinary tract infections and antibiotic resistance has also been studied, and new therapeutic options are being investigated. However, further research is needed to fully understand the implications of the urinary microbiome in urinary tract infections.
Article
Biochemistry & Molecular Biology
Valeria Gaspari, Camilla Ceccarani, Marco Severgnini, Gionathan Orioni, Tania Camboni, Luca Laghi, Sara Morselli, Claudio Foschi, Antonella Marangoni, Clarissa Consolandi, Bianca Maria Piraccini
Summary: This study analyzed the microbiome and metabolome profiles of first-void urines in women with Chlamydia trachomatis (CT) urethral infection. Regardless of CT infection, Lactobacillus was found to dominate the microbiome of first-void urines. During CT infection, samples showed reduced microbial biodiversity and a significant decrease in certain bacterial families and species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Joanna Chorbinska, Wojciech Krajewski, Lukasz Nowak, Bartosz Malkiewicz, Francesco Del Giudice, Tomasz Szydelko
Summary: The urine microbiome, previously thought to be sterile, has been proven to exist and play a role in the pathogenesis of urological diseases. This review aims to summarize the current knowledge on the urinary microbiome in bladder diseases and identify potential directions for further research.
Article
Microbiology
Christopher W. Marshall, Marcia Kurs-Lasky, Christi L. McElheny, Sophia Bridwell, Hui Liu, Nader Shaikh
Summary: The study aimed to assess the impact frequency of 16S rRNA gene amplicon sequencing on diagnosis and clinical management. The results showed high concordance between 16S rRNA gene sequencing and conventional culture results in children with likely and unlikely UTI. In children with possible UTI according to conventional culture, 71% had a single uropathogen at a relative abundance of >= 90% according to 16S rRNA gene sequencing data.
MICROBIOLOGY SPECTRUM
(2021)
Article
Biochemistry & Molecular Biology
Mangesh Suryavanshi, Jose Agudelo, Aaron Miller
Summary: A meta-analysis of microbial communities in urinary stone disease (USD) found that rare phylotypes are prevalent in the gut, upper urinary, and lower urinary tract microbiomes. It was also revealed that rare phylotypes play a significant role in the pathophysiology of USD, particularly in kidney stones. Understanding the contribution of rare and common phylotypes is crucial for the development of bacteriotherapies for urologic health.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Microbiology
Yoon Jae Cho, Bokyoung Shin, Sung-Ha Lee, Sangmin Park, Yoon-Keun Kim, Jae-Jin Kim, Eunjoo Kim
Summary: This study analyzed urine microbiome profiles to explore the potential of the urine microbiome as a biomarker for pediatric ADHD. The ADHD group showed decreased alpha diversity and significant beta diversity differences compared to the control group. The genus Afipia exhibited significant correlations with ADHD symptoms, suggesting its potential as a biomarker for ADHD.
Review
Medicine, General & Internal
Tomislav Mestrovic, Mario Matijasic, Mihaela Peric, Hana Cipcic Paljetak, Anja Baresic, Donatella Verbanac
Summary: The current research focuses on the relationship between gut microbiota and the development of bacteriuria and UTI, as well as the potential impact of vaginal dysbiosis and urinary microbiome perturbations on infections. Further research and exploration are needed to understand and potentially utilize these findings for the diagnosis and treatment of UTIs.
Article
Obstetrics & Gynecology
Margaret G. Mueller, Promi Das, Uduak Andy, Lisa Brennaman, Alexis A. Dieter, Denicia Dwarica, Anna C. Kirby, Jonathan P. Shepherd, W. Thomas Gregory, Cindy L. Amundsen
Summary: This study evaluated the stability of the urinary microbiome communities in women undergoing SNM for UUI. The results showed that the urinary microbiome remained relatively stable, despite variability amongst the cohort.
INTERNATIONAL UROGYNECOLOGY JOURNAL
(2023)
Article
Infectious Diseases
Luciano Garofalo, Claudia Nakama, Douglas Hanes, Heather Zwickey
Summary: Urobiome dysbiosis is a common problem in women with uncomplicated urinary tract infection. Traditional antibiotics are not effective in addressing this issue, so new treatment approaches are needed. A whole-person, multi-modal approach may be more effective in reducing recurrent UTI.
Article
Urology & Nephrology
H. Henry Lai, Jonathan B. Wiseman, Margaret E. Helmuth, Abigail R. Smith, Cindy L. Amundsen, Anne P. Cameron, Alexander P. Glaser, Whitney K. Hendrickson, Ziya Kirkali, Kimberly Kenton
Summary: The purpose of this study was to characterize patients seeking treatment for urinary urgency with or without urgency urinary incontinence. Participants were followed for 1 year, and their symptoms, psychosocial factors, bowel function, sleep disturbance, physical activity levels, physical function, and quality of life were compared. The results showed that patients with urgency urinary incontinence had more severe symptoms, higher levels of psychosocial symptoms, poorer physical functioning, and worse quality of life compared to patients with urinary urgency-only. The study suggested that urgency urinary incontinence may be a more severe manifestation of urinary urgency.
JOURNAL OF UROLOGY
(2023)
Article
Medicine, General & Internal
Thibaut Waeckel, Khelifa Ait Said, Benjamin Menahem, Anais Briant, Arnaud Doerfler, Arnaud Alves, Xavier Tillou
Summary: A 6-year follow-up study of 83 women who underwent bariatric surgery showed that the procedure had long-term effects in improving stress urinary incontinence (SUI) and overactive bladder disorders (OAB), leading to a positive impact on quality of life.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Obstetrics & Gynecology
C. Jung, L. Brubaker
Article
Urology & Nephrology
Kathryn H. Schmitz, Tamara Bavendam, Sonya S. Brady, Siobhan Sutcliffe, Emily Lukacz, Janis M. Miller, Bernard L. Harlow, Kyle Rudser, Aimee James, Kathryn Burgio, Diane Newman, Mary H. Palmer, Linda I. Brubaker
NEUROUROLOGY AND URODYNAMICS
(2020)
Article
Urology & Nephrology
Ariana L. Smith, Leslie M. Rickey, Sonya S. Brady, Cynthia S. Fok, Jerry L. Lowder, Alayne D. Markland, Elizabeth R. Mueller, Siobhan Sutcliffe, Tamara G. Bavendam, Linda Brubaker
Summary: Prevention strategies have been effective in many areas of human health, yet have not been utilized for lower urinary tract symptoms (LUTS) or bladder health (BH). The NIH-sponsored Prevention of Lower Urinary Tract Symptoms Research Consortium (PLUS) aims to enhance traditional prevention science approaches through a transdisciplinary team science approach, mixed methodology, and community engagement. Their foundational work includes developing clear definitions of BH and disease, as well as a BH measurement instrument that will be validated for use in the general population, adolescents, and Latinx and Spanish-speaking women.
Article
Biochemistry & Molecular Biology
L. Brubaker, C. Putonti, Q. Dong, A. J. Wolfe
Summary: Traditionally, the bladder has been considered sterile, but microbial communities have been detected in both adult females and males. These urobiomes may influence the development of lower urinary tract disorders, and there is potential for treating symptoms by modifying the urobiome in the future.
Article
Genetics & Heredity
Carine R. Mores, Travis K. Price, Birte Wolff, Thomas Halverson, Roberto Limeira, Linda Brubaker, Elizabeth R. Mueller, Catherine Putonti, Alan J. Wolfe
Summary: Research found that vaginal intercourse shifts the lower urinary tract microbiota, particularly noting an increase in Streptococcus mitis abundance. Isolates of S. mitis from both partners were genetically similar, indicating potential transmission. This post-coitus bloom in S. mitis may provide a window of opportunity for uropathogens to colonize the urinary tract.
MICROBIAL GENOMICS
(2021)
Article
Microbiology
Linda Brubaker, Jean-Philippe F. Gourdine, Nazema Y. Siddiqui, Amanda Holland, Thomas Halverson, Roberto Limeria, David Pride, Lenore Ackerman, Catherine S. Forster, Kristin M. Jacobs, Krystal J. Thomas-White, Catherine Putonti, Qunfeng Dong, Michael Weinstein, Emily S. Lukacz, Lisa Karstens, Alan J. Wolfe
Summary: The consensus document on urobiome research aims to promote standardization and advancements in the field by adopting common research practices, including standardized nomenclature, specimen handling, and metadata collection. Although fixed analytical procedures may not be practical, guidelines have been proposed to document and report data from urobiome studies.
Article
Obstetrics & Gynecology
Linda Brubaker, Erica Marsh, Marcelle Cedars, Dee Fenner, Amy Murtha, Barbara Goff, Dineo Khabele
Summary: The advancement of women leaders in obstetrics and gynecology does not match the changes in the physician workforce. Gender equity should be a core value in the culture of obstetrics and gynecology, and actions should be taken to prioritize and demonstrate commitment to gender equity. This commentary proposes principles and leadership practices to promote gender equity in academic leadership roles within obstetrics and gynecology.
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2022)
Article
Medical Ethics
Linda Brubaker, Jesse Nodora, Tamara Bavendam, John Connett, Amy M. Claussen, Cora E. Lewis, Kyle Rudser, Siobhan Sutcliffe, Jean F. Wyman, Janis M. Miller
Summary: Authorship and dissemination policies vary among research consortia, and the creation of a repository and toolkit could improve efficiency and integrity in team science work.
ACCOUNTABILITY IN RESEARCH-POLICIES AND QUALITY ASSURANCE
(2022)
Article
Urology & Nephrology
Ariana L. Smith, Kyle Rudser, Bernard L. Harlow, Gerald McGwin, Julia Barthold, Sonya S. Brady, Linda Brubaker, Shayna D. Cunningham, James W. Griffith, Kim Kenton, Heather Klusaritz, Cora E. Lewis, Emily S. Lukacz, Julia Maki, Alayne D. Markland, Elizabeth R. Mueller, Diane K. Newman, Jesse Nodora, Leslie M. Rickey, Todd Rockwood, Melissa Simon, Jean F. Wyman, Siobhan Sutcliffe
Summary: This study aims to understand the distribution of bladder health and the factors that promote bladder health and prevent LUTS in women. It is a population-based longitudinal cohort study conducted in the United States, involving community-dwelling women. Data collection includes self-administered surveys and objective measures. The findings will contribute to the development of interventions for bladder health promotion and LUTS prevention in women.
NEUROUROLOGY AND URODYNAMICS
(2023)
Article
Urology & Nephrology
Linda Brubaker, Julia Barthold, Colleen M. Fitzgerald, Kimberly Kenton, Cora E. Lewis, Jerry Lowder, Emily S. Lukacz, Alayne Markland, Melanie Meister, Janis Miller, Elizabeth R. Mueller, Kyle Rudser, Ariana L. Smith, Diane K. Newman
Summary: This study describes the methods used for the in-person assessment of the RISE FOR HEALTH (RISE) study, a population-based prospective cohort study. Participants undergo various physical assessments, including pelvic floor muscle assessments, musculoskeletal tests, and the Short Physical Performance Battery. They also provide samples for urogenital microbiome analysis. The data collected from this assessment will be used to prioritize future research and prevention strategies.
NEUROUROLOGY AND URODYNAMICS
(2023)
Article
Microbiology
Tanya Kumar, MacKenzie Bryant, Kalen Cantrell, Se Jin Song, Daniel McDonald, Helena M. M. Tubb, Sawyer Farmer, Emily S. S. Lukacz, Linda Brubaker, Rob Knight
Summary: The urogenital microbiome is an important yet understudied human microbiome niche, and urine has been found to contain microorganisms that can affect health. It is crucial to study urine sample storage conditions for scientific rigor and reproducibility.
Article
Education, Scientific Disciplines
Diana Ha, Selam Ghebrendrias, Audra Meadows, Daphne Yvette LaCoursiere, Amanda Lewis, Mark Lawson, Michael Morales, Cynthia Gyamfi-Bannerman, Linda Brubaker
Summary: Structural racism is deeply rooted in academic medical institutions. To address this issue, the Department of Obstetrics, Gynecology and Reproductive Sciences at the University of California, San Diego established a Culture and Justice Quorum. Through various initiatives, the Quorum aims to promote justice and eliminate racism, and has received institutional recognition for its inclusive efforts.
Editorial Material
Medicine, General & Internal
Sarah Averbach, Diana Ha, Audra Meadows, Linda Brubaker, Cynthia Gyamfi-Bannerman
Article
Obstetrics & Gynecology
Linda Brubaker, Harry Horsley, Rajvinder Khasriya, Alan J. Wolfe
Summary: This article presents the case of a 30-year-old woman who seeks specialty consultation for frequent episodes of urinary urgency, frequency, and dysuria. Although her home dipsticks suggest a urinary tract infection (UTI) and her urinalysis shows a moderate number of white blood cells, her urine cultures are always negative. The challenges of this clinical presentation are discussed along with evidence-based evaluation and treatment options.
INTERNATIONAL UROGYNECOLOGY JOURNAL
(2023)