Article
Polymer Science
Manuel Toledano, Manuel Toledano-Osorio, Matthias Hannig, Alvaro Carrasco-Carmona, Maria T. Osorio, Franklin Garcia-Godoy, Inmaculada Cabello, Raquel Osorio
Summary: This narrative review explores the potential effectiveness of using zinc compounds in dentin polymeric adhesives to improve bonding efficacy, remineralization and protection against degradation. The literature search indicates that zinc-doped adhesives promote protection and remineralization of resin-dentin interfaces, contributing to improved sealing efficacy of restorations. The inclusion of zinc in these adhesives increases crystallinity of mineral and collagen crosslinking, inhibiting collagen proteolysis and stabilizing dentin matrix. Zinc not only acts as a protease inhibitor, but also influences signaling pathways and stimulates metabolic effects in dentin mineralization and remineralization processes.
Article
Biotechnology & Applied Microbiology
Xuyan Sheng, Jian Yu, He Liu, Zhejun Wang, Shuli Deng, Ya Shen
Summary: The aim of this study was to investigate the antimicrobial properties of an all-in-one irrigant called Triton, as well as its ability to remove the smear layer in dentin canals. The results showed that Triton demonstrated high antimicrobial activity and effectively removed the smear layer. This indicates that Triton can provide dual benefits of antibiofilm and smear layer removal capabilities, offering a simplified and effective strategy for root canal treatment.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Shikai Zhao, Zhiyi Zhu, Jian Yu, Chenmin Yao, Miaoyang Yu, Hongye Yang, Cui Huang
Summary: This study suggests that the acetone wet-bonding technique can enhance the durability of dentin bonding by improving the wettability of the dentin surface, removing water, improving the penetration of adhesive monomer, and inhibiting collagenolytic activities.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Polymer Science
Graca Maria Abreu Pereira de Brito, Daniella Oliveira Silva, Rayssa Ferreira Cavaleiro Macedo, Michel Wendlinger Cantanhede Ferreira, Jose Bauer, Flavia de Brito Pedroso, Alessandra Reis, Fabiana Suelen Figueredo Siqueira, Alessandro Dourado Loguercio, Andres Felipe Milian Cardenas
Summary: This study evaluated the effect of an additional hydrophobic resin coat associated with universal adhesives on sound and eroded dentin. The results showed that the extra coat improved microtensile bond strength and reduced nanoleakage, particularly in eroded dentin after two years of storage.
Article
Dentistry, Oral Surgery & Medicine
Pedro Henrique Freitas, Carolina Bosso Andre, Bruna Marin Fronza, Marcelo Giannini, Pedro Luiz Rosalen, Simonides Consani, Rodrigo Franca
Summary: Incorporation of doxycycline into dental adhesive showed positive effects on physicochemical properties, bacterial inhibition, and maintenance of resin-dentin bond strength.
JOURNAL OF DENTISTRY
(2021)
Article
Polymer Science
Madalena Belmar da Costa, Antonio H. S. Delgado, Tomas Amorim Afonso, Luis Proenca, Ana Sofia Ramos, Ana Mano Azul
Summary: Comparing the adhesive interface of eroded dentin, the study found that Clearfil SE Protect exhibited similar mechanical and adhesive performance to Clearfil SE Bond 2, with promising long-term results in eroded dentin due to increased enzymatic activity and the need for remineralization.
Article
Energy & Fuels
Ernestine Sefakor Coffie, Liyuan Xie, Muhammad Hassan, Buyin Shi, Ghulam Abbas Ashraf, Bo Zhang
Summary: This study investigated the degradation of norfloxacin antibiotics in the bioelectro-Fenton system using different composite electrodes, demonstrating that Fe3O4Y performed the best in terms of NOR removal. The BEF system showed high efficiency in removing biorecalcitrant pollutants and generating bioelectricity, with negligible residual iron concentration below permissible limits. The microbial community analysis identified Proteobacteria, Firmicutes, and Actinobacteria as dominant phyla, indicating the promising potential of using the BEF system with composite electrodes.
Article
Microbiology
Yihui Wang, Joseph P. Hoffmann, Sarah M. Baker, Kerstin Honer zu Bentrup, William C. Wimley, Joseph A. Fuselier, Jacob P. Bitoun, Lisa A. Morici
Summary: This study shows that outer membrane vesicles (OMVs) derived from Burkholderia thailandensis have antibiofilm activity against Streptococcus mutans, reducing biofilm biomass, biofilm integrity, and bacterial cell viability. Heat-labile and heat-stable components in OMVs contribute to their antibiofilm activity. Co-administration of OMVs with gentamicin enhances the efficacy of the antibiotic against S. mutans biofilms.
Article
Dentistry, Oral Surgery & Medicine
Shiori Yamamoto, Mahmoud Sayed, Motoi Takahashi, Khairul Matin, Noriko Hiraishi, Toru Nikaido, Michael F. Burrow, Junji Tagami
Summary: The study found that the surface roughness of dentin specimens in the S-PRG group was significantly higher compared to the nonS-PRG and control group. The S-PRG group showed prevention of biofilm formation and bacterial adhesion on the dentin surface, as well as the highest acid-resistance ability without surface loss. The application of S-PRG barrier coat on dentin surfaces can inhibit biofilm formation and protect against demineralization.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Biophysics
Maryam Tavafoghi, Sanchit Garg, Anton Korenevski, Maria Dittrich
Summary: This study investigates the potential of phytoglycogen nanoparticles in treating cyanobacterial biofilms, finding that cationized phytoglycogen nanoparticles can strongly inhibit and eradicate biofilms due to their strong electrostatic interactions with cyanobacterial cells. These antibacterial polysaccharide nanoparticles may provide a novel cost-effective and environment-friendly strategy for treating biofilm formation by a broad spectrum of bacteria.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biochemistry & Molecular Biology
C. S. Karthik, M. H. Chethana, H. M. Manukumar, A. P. Ananda, S. Sandeep, S. Nagashree, L. Mallesha, P. Mallu, H. S. Jayanth, B. P. Dayananda
Summary: This study synthesized benzodioxane coupled piperazine decorated chitosan silver nanoparticles (Bcp*C@AgNPs) and evaluated its antibacterial and anti-biofilm properties against methicillin-resistant Staphylococcus aureus (MRSA). The nanoparticles showed effective antibacterial activity and a reduced toxic effect on myoblast cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Nanoscience & Nanotechnology
Fei Tong, Pei Wang, Ziqiang Chen, Yifan Liu, Lianguo Wang, Jun Guo, Zhihua Li, Hu Cai, Junchao Wei
Summary: In this study, Fe3O4 magnetic nanoparticles loading minocycline (FPM NPs) were constructed to physically penetrate and effectively eliminate periodontal biofilm. The results showed that FPM NPs had anti-biofilm activity both in vitro and in vivo, and possessed good biocompatibility. In rat models, FPM NPs improved periodontal tissue inflammation. This study provides experimental support for the clinical application of magnetic-targeted nanoparticles.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Nanoscience & Nanotechnology
Juanjuan Xu, Yuanpei Li, Haojie Wang, Mengxi Zhu, Wenpo Feng, Gaofeng Liang
Summary: The study successfully developed an AMP@PDA@AgNPs nanocomposite with excellent biocompatibility and strong antimicrobial activity, capable of disrupting bacterial biofilms. The nanocomposite showed potential in treating bacterial infections by inhibiting the expression of biofilm-related genes. The synergistic strategy of AMPs and AgNPs provided a new perspective for the treatment of bacterial infections.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Food Science & Technology
Quan Lin, Yanxin Li, Maokun Sheng, Jiaman Xu, Xiaoyan Xu, Jintae Lee, Yulong Tan
Summary: In recent years, there has been an increasing trend in Candida contamination caused by Candida mastitis, which has a significant impact on the hygienic quality of raw milk and dairy products. The biofilm produced by Candida albicans plays a critical role in fungal infection by protecting the fungus from host defense and drug attacks. Therefore, finding effective means to reduce the biofilm and drug resistance of Candida albicans has become an urgent problem. This study investigated the anti-biofilm effect of berberine-loaded chitosan nanoparticles on Candida albicans and explored the antifungal mechanism of released berberine.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Rui Guo, Wenan Peng, Hongye Yang, Chenmin Yao, Jian Yu, Cui Huang
Summary: The study demonstrated that resveratrol-doped adhesive significantly improved the durability of dentin bonding and secondary caries management.
Additionally, the resveratrol-doped adhesive exhibited antibiofilm properties and acceptable biocompatibility.
Therefore, the application of resveratrol-doped adhesive holds promise for increasing the lifetime of composite restorations.
JOURNAL OF DENTISTRY
(2021)
Article
Biochemistry & Molecular Biology
Zhou Ye, Conrado Aparicio
Summary: Antimicrobial peptides show potential in treating multi-drug resistant bacterial infections, with the d-enantiomer GL13K demonstrating higher antimicrobial activity than the l-enantiomer and enhanced activity against Gram-positive bacteria. Both l-GL13K and d-GL13K form self-assembled amyloid-like nanofibrils when interacting with components of bacterial outer membrane and cell wall. Specific interactions between peptidoglycans and d-GL13K may contribute to its enhanced activity against Gram-positive bacteria.
JOURNAL OF PEPTIDE SCIENCE
(2022)
Article
Dentistry, Oral Surgery & Medicine
John A. Pizarek, Nicholas G. Fischer, Conrado Aparicio
Summary: The study synthesized an IL-23R antagonist peptide nanocoating on titanium surfaces to reduce inflammation around dental implants and promote soft tissue healing.
Article
Engineering, Biomedical
Michail Raptopoulos, Nicholas G. G. Fischer, Conrado Aparicio
Summary: Previous studies have shown that the hydrophilic/hydrophobic nature of implant surfaces can either stimulate or hinder osseointegration. This study applied a similar concept by modifying a model surface with common biological functional groups to promote oral keratinocytes (OKs) proliferation and extend implant lifespans through enhanced soft tissue attachment. The results indicate that the functionalization of the surface with the -OH group showed the highest OKs proliferation and hemidesmosomes (HD) expression, suggesting that the physicochemical properties of the surface play a significant role in improving implant soft tissue attachment.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Review
Engineering, Biomedical
Nicholas G. Fischer, Conrado Aparicio
Summary: The article explores etiological factors contributing to percutaneous device infection and the mechanisms by which natural percutaneous organs, such as teeth, prevent infection. It highlights the role of hemidesmosomes and their five main components in preventing percutaneous device infection. The authors propose bioengineering strategies to solve the percutaneous device dilemma and promote interdisciplinary collaboration.
BIOACTIVE MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Alp Sarisoy, Sergio Acosta, Jose Carlos Rodriguez-Cabello, Phillip Czichowski, Alexander Kopp, Stefan Jockenhoevel, Alicia Fernandez-Colino
Summary: Over 260 million surgical procedures are performed worldwide each year. Bioadhesives have been proposed as an alternative to sutures and staples for reconnecting tissues, but a clinically viable adhesive that combines strong adhesion with cytocompatibility has yet to be developed. In this study, researchers explored the production of adhesives based on protein-engineered polymers inspired by elastin. Surprisingly, the adhesion of the protein-engineered polymer alone surpassed that of a combination with tannic acid. Further experiments were conducted to develop a multi-component bioadhesive incorporating elastin and silk fibroin, showing promising adhesive strength and cytocompatibility. These findings open up possibilities for using protein-engineered polymers in tissue engineering applications without additional chemical modification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Jordi Gargallo-Albiol, Nicholas G. Fischer, Conrado Aparicio, Hom-Lay Wang
Summary: The study investigated the effects of polyetheretherketone (PEEK), zirconia (ZrO2), and titanium (Ti) disks on proliferation and hemidesmosome formation of gingival cells. Results showed that cells proliferated faster on Ti and exhibited higher expression of hemidesmosome markers on ZrO2.
INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Manuel Toledano, Estrella Osorio, Maria T. Osorio, Fatima S. Aguilera, Raquel Toledano, Enrique Fernandez-Romero, Raquel Osorio
Summary: This study evaluated the bonding ability and mechanical and chemical behavior of demineralized dentin infiltrated with polymeric nanoparticles doped with dexamethasone. The results showed that dexamethasone nanoparticles improved the nanohardness and Young's modulus of dentin, and promoted remineralization and improved the structure of collagen.
JOURNAL OF DENTISTRY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Alvaro Blasi, Victor Henarejos-Domingo, Ricardo Palacios-Banuelos, Conrado Aparicio, Miguel Roig
Summary: This clinical trial aimed to compare the accuracy of interocclusal registration using polyvinyl siloxane and intraoral scanner (IOS) for the fabrication of occlusal devices. The study included 31 participants who received two different occlusal devices using different workflows. The results showed no significant difference between digital impressions and conventional methods. Therefore, this study provides evidence for the application of digital impressions in complete arch prosthesis.
JOURNAL OF ESTHETIC AND RESTORATIVE DENTISTRY
(2023)
Article
Polymer Science
Zhou Ye, Yipin Qi, Anqi Zhang, Brandon J. Karels, Conrado Aparicio
Summary: The study developed a method to mineralize collagen with Sr-doped hydroxyapatite (HA) using the polymer-induced liquid precursor (PILP) process. Sr doping altered the crystal lattice of HA and inhibited mineralization in a concentration-dependent manner, but did not affect the unique intrafibrillar mineralization using PILP. The Sr-doped HA nanocrystals aligned in the [001] direction but did not replicate the parallel alignment of pure Ca HA with the collagen fiber long axis. The mimicry of Sr doping in PILP-mineralized collagen has important implications for understanding natural hard tissues and treatment processes.
Article
Dentistry, Oral Surgery & Medicine
Alvaro Blasi, Victor Henarejos-Domingo, Ricardo Palacios-Banuelos, Carla Vidal-Ponsoda, Conrado Aparicio, Miguel Roig
Summary: This study aimed to compare the volumetric changes on occlusal surface of occlusal devices fabricated with a fully digital workflow and an analog workflow after occlusal adjustment. Eight participants received two different occlusal devices fabricated with two different workflows. The results showed that occlusal devices fabricated with a fully digital workflow required fewer occlusal adjustments, making them a potential alternative to devices fabricated with an analog workflow.
JOURNAL OF ESTHETIC AND RESTORATIVE DENTISTRY
(2023)
Article
Polymer Science
Antonio L. Medina-Castillo, Modesto T. Lopez-Lopez, Maria Dolores Fernandez-Ramos
Summary: This paper presents, for the first time, the theoretical formulation of the distribution function of critical nuclei under Pre-Poly conditions. It also develops a simple empirical method to calculate the concentration of critical nuclei, which shows good agreement with theoretical calculations.
Article
Pharmacology & Pharmacy
Isha Mutreja, Caixia Lan, Qishun Li, Conrado Aparicio
Summary: Developing antimicrobial surfaces, including antimicrobial peptide coatings, is a promising approach to overcome dental implant-associated infection. This study presents a fast and efficient chemoselective conjugation technique to coat titanium implant surfaces with antimicrobial peptides. The evaluated surfaces showed antibacterial potency against primary colonizers and pathogens of dental surfaces, as well as demonstrated long-term viability of human dental pulp stem cells.
Article
Engineering, Biomedical
Viviana R. Lopes, Ulrik Birgersson, Vivek Anand Manivel, Gry Hulsart-Billstrom, Sara Gallinetti, Conrado Aparicio, Jaan Hong
Summary: The study investigated early interactions between three alloplastic materials (CaP, Ti, and PEEK) and human whole blood. Coagulation was initiated on all test materials, with Ti showing the largest increase. All materials showed increased contact activation. CaP demonstrated the most pronounced activation of the complement system. This complement activation correlated with leukocyte stimulation.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Biophysics
Sunil Kumar Boda, Nora Willkomm, Maria S. Barrera, Louis Mansky, Conrado Aparicio
Summary: This article introduces a method of electrostatic capturing of viruses in the oral cavity using cationic biopolymer membranes as an alternative to traditional swabbing. By coating anionic or cationic substances on chitosan nanofiber membranes, the adsorption efficiency of viruses can be improved.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Meeting Abstract
Cell & Tissue Engineering
Nicholas G. Fischer, David A. De Jong, Conrado Aparicio
TISSUE ENGINEERING PART A
(2022)
