Journal
ACS NANO
Volume 14, Issue 4, Pages 4973-4981Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.0c01174
Keywords
shortwave infrared fluorescence; gold nanoclusters; vascular disorder; Monte Carlo restoration imaging processing; bone morphogenetic protein 9 (Bmp9)
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Funding
- Canceropole Lyon Auvergne Rhone-Alpes (CLARA), Plan Cancer [C18038CS]
- ARC [R17157CC]
- European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant [846764]
- German Research Council (DFG) [RE1203/12-3]
- Institut National de la Santeet de la Recherche Me'dicale (INSERM) [U1036]
- Commissariat a 'l'Energie Atomique et aux Energies Alternatives (CEA, DRF/IRIG)
- University Grenoble Alpes (UGA, BCI)
- Fondation pour la Recherche Medicale (FRM)
- Agence Nationale de la Recherche (ANR) [ANR-17-CE14-0006]
- Labex GRAL (University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS [ANR-10-LABX-49-01, ANR-17-EURE-0003]
- Marie Curie Actions (MSCA) [846764] Funding Source: Marie Curie Actions (MSCA)
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We synthesized a generation of water-soluble, atomically precise gold nanoclusters (Au NCs) with anisotropic surface containing a short dithiol pegylated chain (AuMHA/TDT). The AuMHA/TDT exhibit a high brightness (QY similar to 6%) in the shortwave infrared (SWIR) spectrum with a detection above 1250 nm. Furthermore, they show an extended half-life in blood (t(1/2 beta) = 19.54 +/- 0.05 h) and a very weak accumulation in organs. We also developed a non-invasive, whole-body vascular imaging system in the SWIR window with high-resolution, benefiting from a series of Monte Carlo image processing. The imaging process enabled to improve contrast by 1 order of magnitude and enhance the spatial resolution by 59%. After systemic administration of these nanoprobes in mice, we can quantify vessel complexity in depth (>4 mm), allowing to detect very subtle vascular disorders non-invasively in bone morphogenetic protein 9 (Bmp9)-deficient mice. The combination of these anisotropic surface charged Au NCs plus an improved SWIR imaging device allows a precise mapping at high-resolution and an in depth understanding of the organization of the vascular network in live animals.
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