Article
Agriculture, Multidisciplinary
Ramesh Kumar Dhandapani, Dhandapani Gurusamy, Subba Reddy Palli
Summary: This study evaluated a nanoparticle delivery system based on protamine sulfate (PS)/lipid/dsRNA for gene silencing in the fall armyworm and demonstrated its high efficiency and stability in cells and larvae.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Immunology
Xiaoyue Hou, Lu Han, Baoju An, Jun Cai
Summary: This study revealed that Vip3Aa can induce autophagy in Sf9 cells and identified the AMPK-mTOR-ULK1 pathway involved in this process, suggesting a pro-survival role for autophagy that may be related to insect resistance development.
Article
Entomology
Zijian Chao, Zhongzheng Ma, Yunhui Zhang, Shuo Yan, Jie Shen
Summary: RNA interference (RNAi) is an essential technique in functional analysis of insect genes and pest management. However, insensitivity to RNAi hinders the identification of functional genes in lepidopteran insects. This study developed a nanocarrier-mediated dsRNA delivery system to achieve efficient RNAi in Spodoptera frugiperda. The system showed effective knockdown of key developmental genes and pest control efficiency in the targeted insect species.
ENTOMOLOGIA GENERALIS
(2023)
Article
Chemistry, Multidisciplinary
Erin McGraw, Jonathan D. Roberts, Nitish Kunte, Matthew Westerfield, Xavier Streety, David Held, L. Adriana Avila
Summary: This study investigates the role of endocytosis and transcytosis in the transport of branched amphipathic peptide nanocapsules (BAPCs) associated with dsRNA through midgut epithelium cells. Clathrin-mediated endocytosis and macropinocytosis are found to be responsible for cellular uptake, while transcytosis is involved in shuttling BAPCs-dsRNA from the lumen to the hemolymph.
Article
Ecology
Rong Feng, Liang Chen, Keping Chen
Summary: The study revealed that high concentrations of aluminum can lead to cell enlargement, cell membrane breakage, decreased cell vitality, and apoptosis. It also identified that aluminum ions may inhibit the expression of certain proteins related to cell pathways and induce oxidative damage, ultimately affecting cell growth and apoptosis.
Article
Food Science & Technology
Xuehua Shao, Zhuhong Zhang, Xuhong Qian, Lanying Wang, Yunfei Zhang, Yanping Luo
Summary: Novel furanocoumarin derivatives were synthesized and investigated for their photosensitive activity, showing excellent inhibition of Spodoptera frugiperda cell proliferation. The derivative I34 induced cell apoptosis, increased intracellular ROS level, decreased mitochondrial membrane potential, and blocked cell cycle progression. Additionally, I34 activated the mitochondrial apoptotic signal transduction pathway. These findings highlight the potential application of novel furanocoumarins as biochemical pesticides.
Article
Cell Biology
Zhiping Xu, Lianhua Zhu, Yun Yang, Yang Zhang, Miaoqing Lu, Liming Tao, Wenping Xu
Summary: Bifenthrin, a commonly used synthetic pyrethroid insecticide, targets the nervous system of insects and may lead to pest resistance with high usage. Studies have shown that it induces DNA damage and autophagy, significantly impacting the viability of Spodoptera frugiperda cells. The upregulation of LC3-II and beclin-1 protein expression, along with downregulation of p62 expression, contributes to its cytotoxic effect.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2021)
Article
Plant Sciences
Daniel Endale Gebremichael, Zeraye Mehari Haile, Francesca Negrini, Silvia Sabbadini, Luca Capriotti, Bruno Mezzetti, Elena Baraldi
Summary: The application of exogenous RNAi technology in managing plant pathogenic fungi has shown promising results in reducing disease severity, highlighting the great potential of RNAi-mediated control of fungal diseases in crops.
Article
Multidisciplinary Sciences
Mohammad Vatanparast, Youngjin Park
Summary: The fall armyworm is one of the most destructive insect pests in the world, and it has invaded Africa and spread to Asia within two years. Glycerol has been found to play a crucial role in the rapid cold hardening process, and the genes GPDH and GK are associated with glycerol synthesis. RNA interference of these genes resulted in a decrease in survival rate in the fall armyworm larvae. After a cold period, an increase in glycerol accumulation was observed. This research highlights the importance of glycerol as a cryoprotectant for the survival of this quarantine pest during the cold period.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Biomedical
Molly K. Grun, Alexandra Suberi, Kwangsoo Shin, Teresa Lee, Victoria Gomerdinger, Zoe M. Moscato, Alexandra S. Piotrowski-Daspit, W. Mark Saltzman
Summary: The study found that low concentrations of PACE-PEG can improve polyplex stability but also inhibit transfection efficiency. In vivo, the effect of PACE-PEG incorporation on transfection varied by delivery route, and excess PEGylation was detrimental to vehicle function.
Article
Entomology
Ilaria Di Lelio, Eleonora Barra, Mariangela Coppola, Giandomenico Corrado, Rosa Rao, Silvia Caccia
Summary: Transgenic plants expressing dsRNA targeting essential insect genes have shown to be effective in controlling pests. In this study, tobacco plants were transformed to produce dsRNA targeting Sl 102 gene involved in the immune response of Spodoptera littoralis larvae. Experimental results demonstrated that larvae reared on these transgenic tobacco lines exhibited decreased gene expression, impaired encapsulation response, and increased susceptibility to insecticide. This approach could potentially reduce the use of insecticides and enhance their efficacy against mature larvae.
JOURNAL OF PEST SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Abdullah A. Alamoudi, Paula A. Mendez, David Workman, Andreas G. Schatzlein, Ijeoma F. Uchegbu
Summary: Therapeutic gene silencing in the brain can be achieved through intranasal administration of siRNA-poly(ethylene glycol) based polyplexes. These polyplexes have low toxicity and can effectively silence specific genes in brain cells.
Article
Biotechnology & Applied Microbiology
Henry de Malmanche, Mazhar Hussain, Esteban Marcellin, Steve Reid, Sassan Asgari
Summary: The Sf9 cell line, widely used for the production of recombinant proteins and virus-like particles, has been found to use RNA interference (RNAi) as an antiviral response to baculovirus infection. Knocking out Dicer-2, a key protein in the RNAi pathway, resulted in increased viral replication in Sf9 cells, indicating a potential role for RNAi in managing chronic viral infections in this cell line.
JOURNAL OF GENERAL VIROLOGY
(2022)
Review
Engineering, Biomedical
Anna Zahoranova, Robert Luxenhofer
Summary: Poly(2-oxazoline)s and poly(2-oxazine)s, as two closely related families of polymers, have gained increasing attention in recent years, with significant potential in macromolecular self-assemblies and non-covalent drug delivery systems. The extensive synthetic toolbox they offer suggests a high potential for further development in the field.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Youngjin Park, Mohammad Vatanparast
Summary: This study found that the PBAN receptor plays an important role in the females of the fall armyworm, and specific RNA interference can significantly reduce the fecundity of the females.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yue Gao, Suning Liu, Qiangqiang Jia, Lixian Wu, Dongwei Yuan, Emma Y. Li, Qili Feng, Guirong Wang, Subba R. Palli, Jian Wang, Sheng Li
Summary: The study reveals that JH membrane signaling phosphorylates USP at Ser35 through PKC activation, enhancing the regulation of 20E on fruit fly development. The uspS35A mutant with Ala substitution at Ser35 shows reduced body size and delayed developmental timing.
Article
Entomology
Xien Chen, Subba Reddy Palli
Summary: The study identified the molecular mechanism of deltamethrin resistance in fall armyworm by showing the key role of P450 gene SfCYP321A8, which could be applied in resistance and functional genomics research. Transgenic lines expressing P450 genes were developed and showed significantly increased tolerance to deltamethrin through bioassays, highlighting the potential for further research on insecticide resistance in lepidopteran pests.
JOURNAL OF PEST SCIENCE
(2022)
Article
Entomology
Xien Chen, Subba Reddy Palli
Summary: A piggyBac-based germline transformation system was established in the fall armyworm, Spodoptera frugiperda, a global agricultural pest. Tissue-specific promoters were identified and used to drive the expression of transgenes, resulting in tissue-specific overexpression. Overexpression of certain genes in the midgut was found to confer insecticide resistance.
JOURNAL OF PEST SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Ramesh Kumar Dhandapani, Dhandapani Gurusamy, Subba Reddy Palli
Summary: This study evaluated a nanoparticle delivery system based on protamine sulfate (PS)/lipid/dsRNA for gene silencing in the fall armyworm and demonstrated its high efficiency and stability in cells and larvae.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Entomology
Xien Chen, Subba Reddy Palli
Summary: This study reports the establishment of multiple transgenic CRISPR/Cas9-based genome editing methods in the global agricultural pest, fall armyworm, Spodoptera frugiperda. Fluorescent proteins suitable for screening for transgenic fall armyworm were identified. The use of U6-tRNA-sgRNA system for sgRNA expression was found to be more efficient in inducing target gene knockout. These methods provide valuable tools for gene editing and functional genomics studies in the fall armyworm and other lepidopteran pests.
JOURNAL OF PEST SCIENCE
(2023)
Review
Entomology
Marc Kenis, Giovanni Benelli, Antonio Biondi, Paul-Andre Calatayud, Roger Day, Nicolas Desneux, Rhett D. Harrison, Darren Kriticos, Ivan Rwomushana, Johnnie van den Berg, Francois Verheggen, Yong-Jun Zhang, Lakpo Koku Agboyi, Regis Besmer Ahissou, Malick N. Ba, Julio Bernal, Adeney de Freitas Bueno, Yves Carriere, Geraldo Andrade Carvalho, Xue-Xin Chen, Lizette Cicero, Hannalene du Plessis, Regan Early, Patrick Fallet, Komi K. M. Fiaboe, Dnyaneshwar M. Firake, Georg Goergen, Astrid T. Groot, Raul N. C. Guedes, Ankita Gupta, Gao Hu, Fn Huang, Lara R. Jaber, Edi A. Malo, Christina B. McCarthy, Robert L. Meagher, Samira Mohamed, David Mota Sanchez, Rodney N. Nagoshi, Nicolas Negre, Saliou Niassy, Noboru Ota, Casper Nyamukondiwa, Celso Omoto, Subba Reddy Palli, Roman Pavela, Ricardo Ramirez-Romero, Julio C. Rojas, Sevgan Subramanian, Bruce E. Tabashnik, Wee Tek Tay, Eduardo Gabriel Virla, Su Wang, Trevor Williams, Lian-Sheng Zang, Lisheng Zhang, Kongming Wu
Summary: This article provides a comprehensive review of the fall armyworm, including its biology, worldwide status and spread, management strategies, and recommendations for sustainable management in invaded regions.
ENTOMOLOGIA GENERALIS
(2023)
Article
Agronomy
Xingeng Wang, Jessica Faucher, Ramesh Kumar Dhandapani, Jian J. Duan, Subba Reddy Palli
Summary: This study investigated the impact of dsRNA-based insecticides on the survival and behavior of a parasitoid wasp. The results showed that direct injection of dsRNA did not affect the wasp's survival, but it indirectly affected the suitability and detectability of host larvae.
PEST MANAGEMENT SCIENCE
(2023)
Article
Infectious Diseases
Anna R. Pasternak, Subba R. Palli
Summary: Kentucky experiences a high incidence of ehrlichiosis, primarily transmitted by the lone star tick. Surveillance of tick distribution and pathogen detection is crucial for monitoring the expansion of this important tick vector and the increase in tick-borne diseases.
TICKS AND TICK-BORNE DISEASES
(2023)
Article
Engineering, Environmental
Lin Liu, Olga V. Tsyusko, Jason M. Unrine, Shuang Liu, Yidan Liu, Lulu Guo, Gehong Wei, Chun Chen
Summary: The effects of zinc oxide nanoparticles and sulfidized zinc oxide nanoparticles on the carbon cycling in the legume rhizosphere were investigated. It was found that these nanoparticles significantly increased the dissolved organic carbon concentrations and stimulated the growth of microbes involved in organic matter degradation. The results provide new insights into the ecological functions of nanoparticles in soil-plant systems.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anye Wamucho, Jason Unrine, John May, Olga Tsyusko
Summary: Previous studies have demonstrated multigenerational and transgenerational reproductive toxicity in Caenorhabditis elegans after exposure to silver nanoparticles (Ag-NPs) and silver ions (AgNO3). However, the mechanism of transfer of increased reproductive sensitivity to subsequent generations is not well understood. This study investigates the changes in DNA methylation at the epigenetic mark N6-methyl-2'-deoxyadenosine (6mdA) after multigenerational exposure of C. elegans to pristine and sulfidized Ag-NPs and AgNO3. The results suggest the involvement of an epigenetic mark in AgNO3 reproductive toxicity and indicate potential differences in toxicity mechanisms between AgNO3 and Ag-NPs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Heng Jiang, Xiangkun Meng, Nan Zhang, Huichen Ge, Jiaping Wei, Kun Qian, Yang Zheng, Yoonseong Park, Subba Reddy Palli, Jianjun Wang
Summary: This study found that deltamethrin-induced oxidative stress activates AMPK, promoting the nuclear translocation of CncC through phosphorylation. CncC acts as a transcription activator of detoxification genes and regulates the expression of genes involved in ecdysteroid biosynthesis and juvenile hormone degradation. This results in increased ecdysteroid levels, decreased juvenile hormone titer, and down-regulated vitellogenin gene expression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Jinmo Koo, Dhandapani Gurusamy, Subba Reddy Palli
Summary: There is limited success in using exogenous small interfering RNA (siRNA) or small hairpin RNA (shRNA) to trigger RNA interference (RNAi) in insects. Instead, long double-stranded RNA (dsRNA) is used to induce gene knockdown in insects. In this study, the potency of si/sh RNAs and dsRNA was compared in Colorado potato beetle (CPB) cells. CPB cells showed a highly efficient RNAi response to dsRNA, but si/sh RNAs were inefficient in triggering RNAi in CPB cells.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Chemistry, Inorganic & Nuclear
Richard J. Mitchell, Anitha S. Gowda, Alexander G. Olivelli, Aron J. Huckaba, Sean Parkin, Jason M. Unrine, Viral Oza, Jessica S. Blackburn, Folami Ladipo, David K. Heidary, Edith C. Glazer
Summary: Cancer cells rely on glycolysis for energy but recent studies have shown that their mitochondria still play an active role. The study synthesized and characterized ruthenium compounds with triarylphosphine-containing bipyridyl ligands which showed high depolarizing capabilities on mitochondrial membranes in cancer cells. These compounds have the potential for anticancer applications due to their potency against cancer cells and low toxicity in zebrafish embryos.
INORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yaoyu Jiao, Subba Reddy Palli
Summary: m6A methylation is a common modification of mRNA, which regulates multiple stages of mRNA processing. In this study, the role of m6A modification in insect development was explored using the red flour beetle as a model. Knockdown of m6A writers and readers disrupted ecdysis during eclosion, sterilized the insects, and affected egg laying and embryonic development.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Richard J. J. Mitchell, Sarah M. M. Kriger, Alexander D. D. Fenton, Dmytro Havrylyuk, Ankit Pandeya, Yang Sun, Tami Smith, Jason E. E. DeRouchey, Jason M. M. Unrine, Viral Oza, Jessica S. S. Blackburn, Yinan Wei, David K. K. Heidary, Edith C. C. Glazer
Summary: A potent Ru(ii) agent with cytotoxicity in cancer cells and minimal toxicity in zebrafish embryos has been designed as a monofunctional anticancer agent, mimicking the biological effects of phenanthriplatin.
RSC CHEMICAL BIOLOGY
(2023)