Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 12, Issue 20, Pages 22624-22629Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.0c04536
Keywords
Bi2Te3 nanosheets; CdTe quantum dots; photoelectrochemical biosensor; DNA amplifying strategies; sensitive detection
Funding
- Fundamental Research Funds for the Central Universities [XDJK2020TY002, XDJK2019B022, XDJK2018C041]
- National Natural Science Foundation (NNSF) of China [51473136, 21575116]
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In this work, Bi2Te3 nanosheets treated with N-vinyl-pyrrolidinone showed highly sufficient and stable photo-current for being used as a novel photoactive material. Accordingly, with CdTe quantum dots (QDs) sensitizing Bi2Te3 nanosheets, photoelectrochemical (PEC) biosensor coupling of DNA-amplifying strategies was constructed for sensitive miRNA-21 detection. Initially, the Bi2Te3 nanosheets on the electrode have conductive surface states with dissipationless electronic property, thus providing a highly stable photocurrent and a large surface-to-volume ratio. Then, with the participation of target miRNA-21 and auxiliary DNA, strand displacement amplification took place, thereby opening substantial DNA hairpins for triggering the next hybridization chain reaction (HCR). Through the HCR, long DNA tails decorated with CdTe QDs could thus be assembled on the electrode for enhancing the photocurrent of Bi2Te3 nanosheets. As a result, the proposed PEC biosensor showed a wide detection range from 10 fM to 100 pM with a detection limit of 3.3 fM, displaying a promising avenue to construct simple, ultrasensitive, and stable analytical techniques and tremendous potential in bioanalysis and early clinical diagnosis.
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