Article
Cell Biology
Ryo Kunimatsu, Tomoka Hiraki, Kodai Rikitake, Kengo Nakajima, Nurul Aisyah Rizky Putranti, Takaharu Abe, Kazuyo Ando, Ayaka Nakatani, Shuzo Sakata, Kotaro Tanimoto
Summary: This study assessed the effects of conditioned medium derived from human deciduous dental pulp-derived mesenchymal stem cells on various cell types. It was found that the conditioned medium enhanced angiogenic potential and bone tissue regeneration.
Article
Neurosciences
Ziying Zhu, Xiaona Zhang, Haojie Hao, Heran Xu, Jun Shu, Qian Hou, Min Wang
Summary: Exosomes derived from umbilical cord mesenchymal stem cells (UC-MSCs) play a key role in promoting wound repair and skin regeneration by recruiting and stimulating fibroblasts to secrete nerve growth factors (NGFs) for skin nerve repair.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Lin Xia, Chen Zhang, Kaiming Su, Jiangang Fan, Yuguang Niu, Yafeng Yu, Renjie Chai
Summary: Neural stem cell therapy is a promising treatment for neurodegenerative disorders, and the oriented growth of neurons is a crucial factor in neural stem cell-based nerve regeneration. Various biomaterials have been used to facilitate the oriented growth of neural stem cell-derived neurons, but magnetic materials are less commonly used compared to other materials. In this study, magnetic nanochains were developed to guide the oriented growth of neural stem cell-derived neurons, showing great potential.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Christina Bogensperger, Julia Hofmann, Franka Messner, Thomas Resch, Andras Meszaros, Benno Cardini, Annemarie Weissenbacher, Rupert Oberhuber, Jakob Troppmair, Dietmar ofner, Stefan Schneeberger, Theresa Hautz
Summary: Transplantation is limited by the shortage of healthy donor organs, but ex situ machine perfusion has the potential to expand the donor pool. Mesenchymal stem cells are considered as a tool with the potential to improve organs, but clinical benefits have not been conclusively demonstrated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Andrea Lavorato, Stefania Raimondo, Marina Boido, Luisa Muratori, Giorgia Durante, Fabio Cofano, Francesca Vincitorio, Salvatore Petrone, Paolo Titolo, Fulvio Tartara, Alessandro Vercelli, Diego Garbossa
Summary: The study discusses the features and applications of different types of mesenchymal stem cells in peripheral nerve regeneration after nerve injury. ADSCs are considered the most suitable MSCs for recovery from peripheral nerve lesions due to their excellent support and stimulation of axonal growth, remarkable paracrine activity, differentiation potential, low immunogenicity, and high survival rate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Gensuke Okamura, Kosuke Ebina, Makoto Hirao, Ryota Chijimatsu, Yasukazu Yonetani, Yuki Etani, Akira Miyama, Kenji Takami, Atsushi Goshima, Hideki Yoshikawa, Takuya Ishimoto, Takayoshi Nakano, Masayuki Hamada, Takashi Kanamoto, Ken Nakata
Summary: Adding basic fibroblast growth factor (bFGF) to the culture of synovial mesenchymal stem cells (SMSC) can significantly promote cartilage regeneration in vivo, leading to better cartilage repair outcomes and more obvious cartilage matrix formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Elsa Gonzalez-Cubero, Maria Luisa Gonzalez-Fernandez, Maria Rodriguez-Diaz, Marta Palomo-Irigoyen, Ashwin Woodhoo, Vega Villar-Suarez
Summary: The study investigated the therapeutic benefits of adipose tissue derived-mesenchymal stem cells (ASCs) and ASC-conditioned medium (CM) in promoting myelination and axonal regeneration. Results showed that both ASCs and CM could potentially aid in nerve regeneration, especially in reversing the effects of TNF-induced nerve damage on myelination-related genes and proteins.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Polymer Science
Yuye Huang, Kailei Xu, Jingyi Liu, Guangli Dai, Jun Yin, Peng Wei
Summary: This study investigated the co-culture of Schwann cells (SCs) and rat adrenal pheochromocytoma (PC-12) cells on a 50% degree of methacryloyl substitution gelatin methacrylate (GelMA) scaffold. PC-12 cells showed enhanced cell proliferation and axonal extension in the presence of neurotrophic factors secreted by SCs. A 5% GelMA scaffold was found to better support SCs proliferation, viability, and myelination compared to a 10% GelMA scaffold.
Review
Biochemistry & Molecular Biology
David A. Hart, Norimasa Nakamura
Summary: After injuries to musculoskeletal tissues, an inflammatory response occurs to promote endogenous repair, but it often results in functionally inferior scar tissue. Injuries in adults no longer have the anabolic processes that contribute to growth and maturation, which can lead to degenerative conditions such as osteoarthritis. Researchers have been exploring cellular therapies, such as Platelet-rich Plasma and mesenchymal stem/medicinal signaling cells, to enhance tissue repair and regeneration. However, implanting these cells or engineered constructs into an inflammatory adult environment may compromise their potential for regeneration. Therefore, it may be necessary to neutralize the inflammatory environment and create an anabolic environment for optimal outcomes.
Article
Engineering, Multidisciplinary
Haixing Xu, Yi Yu, Lingxi Zhang, Furong Zheng, Yixia Yin, Yuxiang Gao, Kebi Li, Jingyi Xu, Jing Wen, Hui Chen, Zhixin Lei, Zhijun Huang, Dan Yu, Nan Jiang, Peihu Xu
Summary: The study investigates the repair of injured peripheral nerves in rats using PDLLA/beta-TCP nerve conduits laden with CS-HA/NGF hydrogels, showing that the hydrogels promote adhesion, spreading, and differentiation of neuronal cells while sustaining NGF release. In vivo studies demonstrate a significant positive effect on axon regeneration and myelination compared to control groups, indicating the potential of CS-HA/NGF injectable hydrogel in promoting nerve regeneration in neural tissue engineering.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Ophthalmology
Qianwen Gong, Shasha Zhang, Lu Jiang, Meng Lin, Zhiqiang Xu, Ye Yu, Qinmei Wang, Fan Lu, Liang Hu
Summary: This study compared the effects of nerve growth factor (NGF), hycosan, and normal saline (NS) treatments on dry eye symptoms following LASIK surgery. The results showed that NGF treatment significantly accelerated corneal nerve regeneration and recovery of sensitivity, outperforming hycosan and NS treatments. Additionally, NGF treatment improved tear breakup time and increased nerve fiber quantity compared to the other treatments.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Engineering, Biomedical
Ohan S. Manoukian, Swetha Rudraiah, Michael R. Arul, Jenna M. Bartley, Jiana T. Baker, Xiaojun Yu, Sangamesh G. Kumbar
Summary: The study investigates novel nerve guidance conduits with aligned microchannel porosity delivering sustained-release of neurogenic 4-aminopyridine for peripheral nerve regeneration. Results show equivalency with autograft controls in terms of functional recovery, myelin development, and histological assessments. The aligned microchannel architecture of the conduit guides axons and the sustained release of 4-AP increases nerve conduction.
BIOACTIVE MATERIALS
(2021)
Article
Neurosciences
Li-Kang Zhang, Jia-Hui Sun, Sheng Liu, Wen-Liang Zhang, Jian-Long Zou
Summary: In this study, we developed an in vitro model called SCWR to mimic the tissue-specific differences between motor and sensory nerve regeneration. The model demonstrated asymmetric regeneration of the ventral and dorsal roots in response to growth factors and exhibited motor and sensory Schwann cell phenotypes. Additionally, we identified a targeted effect of collagen VI on sensory nerve bundle formation and characterized the protein expression profiles associated with motor/sensory-specific nerve regeneration.
EXPERIMENTAL NEUROLOGY
(2023)
Article
Neurosciences
Yunsong Zhang, Yinying Shen, Li Zhao, Qian Zhao, Lili Zhao, Sheng Yi
Summary: Nerve injury-induced Schwann cell dedifferentiation promotes axon growth by creating a favorable microenvironment. Transcription factor BCL11A plays a crucial role in Schwann cell reprogramming during peripheral nerve regeneration. Silencing Bcl11a reduces Schwann cell viability, proliferation, migration rates, and debris clearance ability. Bcl11a deficiency leads to restricted axon elongation and myelin wrapping, resulting in failed recovery. Mechanistically, BCL11A regulates Schwann cell activity by binding to the Nr2f2 promoter and modulating Nr2f2 expression. In conclusion, BCL11A is essential for Schwann cell activation and peripheral nerve regeneration, making it a potential therapeutic target for peripheral nerve injury treatment.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Cell & Tissue Engineering
Sudan Puri, Isabel Y. Moreno, Mingxia Sun, Sudhir Verma, Xiao Lin, Tarsis F. Gesteira, Vivien J. Coulson-Thomas
Summary: This study found that a hyaluronan (HA) matrix is present in the niche surrounding limbal epithelial stem cells (LESCs) during ex vivo expansion. The presence of both endogenous and exogenous HA was shown to help maintain the LESC phenotype and enhance proliferation, colony formation, and expression of stem cell markers. These findings suggest that HA could be a cost-effective substrate or supplement for culturing LESCs in clinical settings.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Polymer Science
Paul Lee, Katelyn Tran, Wei Chang, Ya-Lin Fang, Gan Zhou, Radoslaw Junka, Namdev B. Shelke, Xiaojun Yu, Sangamesh G. Kumbar
POLYMERS FOR ADVANCED TECHNOLOGIES
(2015)
Article
Chemistry, Physical
Paul Lee, Katelyn Tran, Gan Zhou, Asheesh Bedi, Namdev B. Shelke, Xiaojun Yu, Sangamesh G. Kumbar
Article
Chemistry, Multidisciplinary
Paul Lee, Ohan S. Manoukian, Gan Zhou, Yuhao Wang, Wei Chang, Xiaojun Yu, Sangamesh G. Kumbar
Article
Engineering, Biomedical
Munish B. Shah, Wei Chang, Gan Zhou, Joseph S. Glavy, Thomas M. Cattabiani, Xiaojun Yu
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2019)
Article
Biophysics
Xiaqing Zhou, Gan Zhou, Radoslaw Junka, Ningxiao Chang, Aneela Anwar, Haoyu Wang, Xiaojun Yu
Summary: Artificial bone grafts offer advantages in promoting cell proliferation and differentiation in bone tissue regeneration, while using 3D printing technology combined with bio-fabrication methods to prepare porous spiral scaffolds can significantly improve cell growth and differentiation.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Engineering, Biomedical
Wei Chang, Munish B. Shah, Gan Zhou, Kevin Walsh, Swetha Rudraiah, Sangamesh G. Kumbar, Xiaojun Yu
BIOMEDICAL MATERIALS
(2020)
Article
Engineering, Biomedical
Gan Zhou, Wei Chang, Xiaojun Yu
REGENERATIVE ENGINEERING AND TRANSLATIONAL MEDICINE
(2018)