Journal
FOODS
Volume 8, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/foods8120673
Keywords
Allium sativum; beta-carboline alkaloids; anti-adipogenic effects; 3T3-L1 preadipocytes; Ac-alpha-tubulin
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Funding
- National Research Foundation of Korea (NRF) - Korean government (MSIT) [2018R1A2B2006879, 2019R1A5A2027340, 2017R1A6A3A04001986, 2019R1I1A1A01058903]
- National Research Foundation of Korea [2017R1A6A3A04001986, 2019R1I1A1A01058903] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Garlic (Allium sativum L.) is utilized worldwide for culinary and medicinal use and has diverse health benefits. As part of our ongoing research to identify bioactive components from natural resources, phytochemical analysis of the methanolic extract of garlic led to the isolation and characterization of six compounds: Three eugenol diglycosides (1-3) and three beta-carboline alkaloids (4-6). In particular, the absolute configurations of beta-carboline alkaloids (5 and 6) were established by gauge-including atomic orbital nuclear magnetic resonance chemical shift calculations, followed by DP4+ analysis. Here, we evaluated the effects of compounds 1-6 on 3T3-L1 preadipocyte adipogenesis and lipid metabolism. 3T3-L1 adipocyte differentiation was evaluated using Oil Red O staining; the expression of adipogenic genes was detected using RT-qPCR. Among compounds 1-6, (1R,3S)-1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid (6) inhibited 3T3-L1 preadipocyte adipogenesis and reduced the expression of adipogenic genes (Fabp4, PPAR gamma, C/EBP beta, Adipsin, and Adipoq). Moreover, it markedly decreased the actylation of alpha-tubulin, which is crucial for cytoskeletal remodeling during adipogenesis. Anti-adipogenic effects were observed upon treatment with compound 6, not only during the entire process, but also on the first two days of adipogenesis. Additionally, treatment with compound 6 regulated the expression of genes involved in adipocyte lipid metabolism, decreasing the lipogenic gene (SREBP1) and increasing lipolytic genes (ATGL and HSL). We provide experimental evidence of the health benefits of using (1R,3S)-1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid obtained from garlic to prevent excessive adipogenesis in obesity.
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