Article
Agriculture, Dairy & Animal Science
Claudianny Souto Maior de Moraes Vilar, Jessica Moraes Malheiros, Pollyana Ferreira da Silva, Eduardo Henrique Martins, Luiz Eduardo Cruz dos Santos Correia, Matheus Henrique Vargas de Oliveira, Luiz Alberto Colnago, Josineudson Augusto I. I. de Vasconcelos Silva, Maria Eugenia Zerlotti Mercadante
Summary: The aim of this study was to identify and quantify the metabolites in the pectoralis major muscle of male red winged tinamou selected for growth traits. The results showed that the selection index had an impact on the metabolite concentrations in red-winged tinamou.
Article
Chemistry, Analytical
Karsten Seeger
Summary: A new sample preparation method was proposed to shorten the preparation time of NMR samples, suitable for different sample types. Experimental results showed that this extraction protocol combined with slice-selection NMR experiments can be successfully used for metabolic investigations.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Silke S. Heinzmann, Melanie Waldenberger, Annette Peters, Philippe Schmitt-Kopplin
Summary: The study utilizes cluster analysis approach to guide peak assignment in NMR spectra via statistical correlations, providing important information for metabolite annotation; Unknown peaks forming hypotheses with known peaks facilitate metabolite identification; Metabolite identification is performed through database search, 2D NMR analysis and standard spiking, increasing identification yield in NMR spectra.
Article
Food Science & Technology
Nara Regina Brandao Consolo, Alessandra Fernandes Rosa, Luis Carlos Garibaldi Simon Barbosa, Paul Haydon Maclean, Angel Higuera-Padilla, Luiz Alberto Colnago, Evaldo Antonio Lencioni Titto
Summary: This preliminary study aimed to evaluate meat quality properties, muscle metabolite profile, and metabolic pathways associated with dark cutting meat in Angus x Nellore crossbreed cattle. The results showed that dark cutting meat exhibited distinct meat quality attributes and concentrations of post-mortem glycolytic metabolites compared to normal meat, and appeared to be correlated with mitochondrial activity. Pathway analysis indicated that differentiation between dark cutting and normal meat was linked to energetic pathways such as starch and sucrose metabolism, amino sugar, nucleotide sugar metabolism, and glycolysis or gluconeogenesis.
Article
Chemistry, Analytical
Silvio Waschina, Karsten Seeger
Summary: Imaging the distribution of metabolites is important in diagnostics and research. NMR spectroscopy is limited to larger structures, while mass spectrometry imaging is well-established with smaller pixel sizes. To overcome time-consuming extraction procedures, a new protocol was established to complement NMR metabolomics.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biotechnology & Applied Microbiology
Felipe E. Carvalho, Jose Bento S. Ferraz, Victor B. Pedrosa, Elisangela C. Matos, Joanir P. Eler, Marcio R. Silva, Jose D. Guimaraes, Fernando O. Bussiman, Barbara C. A. Silva, Fernando A. Cancado, Henrique A. Mulim, Rafael Espigolan, Luiz F. Brito
Summary: Given the economic importance of fertility and reproductive traits in the beef cattle industry, understanding their genetic background and developing effective breeding strategies are crucial. This study estimated variance components, heritability, and genetic correlations for fertility, female precocity, and semen production and quality traits in Nellore cattle using genomic information. The findings highlight the heritability and genetic relationships among these traits, providing valuable information for breeding programs to improve reproductive performance in Nellore cattle.
Article
Biochemistry & Molecular Biology
Sujin Lee, Ja Yoon Ku, Byeong Jin Kang, Kyung Hwan Kim, Hong Koo Ha, Suhkmann Kim
Summary: This study investigated the urinary metabolic differences in male patients with prostate cancer, bladder cancer, and renal cell carcinoma to find distinct metabolites and potential biomarkers among the three cancers. The results showed significant differences in metabolite levels, particularly 4-hydroxybenzoate, N-methylhydantoin, creatinine, glutamine, and acetate. Receiver operating characteristic analysis demonstrated good discriminatory accuracy between the different types of cancers. This preliminary study highlights the potential of metabolomics in the exploration of human urine for cancer detection.
Article
Biochemistry & Molecular Biology
Raja Ganesan, Anirban Goutam Mukherjee, Abilash Valsala Gopalakrishnan, Vasantha-Srinivasan Prabhakaran
Summary: In this study, the effect of chitosan on metabolite alteration in zebrafish tissue was investigated using solid-state H-1 nuclear magnetic resonance spectroscopy. The results showed that chitosan significantly impacted the metabolism of various molecules in zebrafish, including amino acids, glutathione, and trimethylamine. The study highlights the potential of chitosan-applied chemical metabolomics for discovering bioactive metabolites and gaining new insights into biological processes.
Article
Mathematics, Interdisciplinary Applications
Andreas Heinecke, Lifeng Ye, Maria De Iorio, Timothy Ebbels
Summary: Two-dimensional NMR methods are popular in metabolomics for accurately identifying and quantifying metabolites, but existing processing methods lack information utilization and uncertainty measures. Researchers propose a Bayesian method for analyzing 2D JRES data, allowing for automatic deconvolution, identification, and quantification of metabolites.
Article
Agriculture, Dairy & Animal Science
N. R. B. Consolo, V. L. M. Buarque, J. Silva, M. D. Poleti, L. C. G. S. Barbosa, A. Higuera-Padilla, J. F. M. Gomez, L. A. Colnago, D. E. Gerrard, A. Saran Netto, S. L. Silva
Summary: This study investigated metabolite profiling in liver and muscle of Nellore males with low or high residual feed intake to identify physiological changes. Results showed that different metabolic compounds in liver and muscle correlated with energy, protein, and fat metabolism, affecting residual feed intake and carcass traits in the animals.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2021)
Review
Food Science & Technology
Valentina Maestrello, Pavel Solovyev, Luana Bontempo, Luisa Mannina, Federica Camin
Summary: Extra virgin olive oil (EVOO) is a high-quality product that has become a target of food fraud in recent years. The use of nuclear magnetic resonance (NMR) spectroscopy is reviewed as a reliable and rapid tool to authenticate EVOOs and detect adulteration and mislabeling.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
Article
Biochemistry & Molecular Biology
Li Gan, Yuga Inamura, Yu Shimizu, Yuki Yokoi, Yuki Ohnishi, Zihao Song, Yasuhiro Kumaki, Takashi Kikukawa, Makoto Demura, Masaaki Ito, Tokiyoshi Ayabe, Kiminori Nakamura, Tomoyasu Aizawa
Summary: In this study, analysis of gut microbiota and metabolites in mice treated with mulberry leaf powder (MLP) revealed that long-term MLP consumption can alter gut metabolites without affecting the normal gut microbiota. These findings provide valuable information for considering MLP as a healthy food source.
Article
Fisheries
Hua Yang, Xueni Qiu, Yun Zhao, Yangfang Ye, Weiwei Song, Changkao Mu, Ronghua Li, Chunlin Wang
Summary: This study investigated the optimal anesthetic concentration for cuttlefish using NMR and HPLC analysis. They found that ethanol at 10-30 mL L-1 successfully anesthetized cuttlefish, with recovery observed after 60 minutes at 10 mL L-1 concentration. Cuttlefish also reached a sedative state below 5 mL L-1 ethanol, with a maximum exposure time of 270 minutes. The action of ethanol led to changes in neurotransmitter levels, with increased inhibitory neurotransmitters and decreased excitatory neurotransmitters observed.
Article
Food Science & Technology
Bohai Li, Fan Hui, Zhang Yuan, Qingxiang Shang, Guo Shuai, Yingshu Bao, Yongfu Chen
Summary: This study aimed to investigate the biochemical mechanism of the lipid-lowering effect of consuming koumiss daily by analyzing the fecal metabolomes of hyperlipidemia patients before and after koumiss treatment. Ten significant differential metabolites were detected, showing increased concentrations at days 30 and 60, many of which were involved in lipid metabolism and transport. The metabolites identified were likely associated with the observed lipid-lowering effect.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Oncology
Annabel Steiner, Stefan Andreas Schmidt, Cara Sophie Fellmann, Johannes Nowak, Chin-Lee Wu, Adam Scott Feldman, Meinrad Beer, Leo L. Cheng
Summary: Prostate cancer is the second most diagnosed cancer in men. Current diagnostic standards lack reliability in detecting and characterizing prostate cancer. This study found that metabolomic information can differentiate between benign and malignant prostate tissue, and also predict future prostate cancer diagnosis, making it helpful for early detection.
Article
Agriculture, Dairy & Animal Science
Jefferson R. Gandra, Cibeli A. Pedrini, Nara R. B. Consolo, Anderson P. Acosta, Leonardo O. Seno, Luis C. G. S. Barbosa, Isabelle Z. Noia, Vicente L. M. Buarque, Angel R. H. Padilla, Luis A. Colnago, Erika R. S. Gandra
Summary: This study found that adding probiotics to the pre-weaning diet of Senepol calves can improve their performance and weight at weaning, as well as increase muscle and fat deposition on the carcass. Furthermore, probiotics also enhance protein and fatty acid metabolism, immune system response, and rumen development.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Nara R. B. Consolo, Juliana Silva, Vicente M. Buarque, Luis C. Barbosa, Angel H. Padilla, Luiz A. Colnago, Arlindo Saran Netto, David E. Gerrard, Saulo L. Silva
Summary: The genetic potential for post-weaning muscularity did not impact performance, carcass traits, and meat quality, but it was correlated with serum lipid and protein metabolites, as well as energy metabolism in meat.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Juan F. Morales Gomez, Daniel S. Antonelo, Mariane Beline, Bruna Pavan, Danilo B. Bambil, Paulo Fantinato-Neto, Arlindo Saran-Netto, Paulo Roberto Leme, Rodrigo S. Goulart, David E. Gerrard, Saulo L. Silva
Summary: The study found that the growth rate has a greater impact on muscle and fat deposition in feedlot-finished animals, but a smaller impact on pasture-finished animals. Feedlot animals had higher Longissimus muscle area, backfat thickness, meat luminosity, and tenderness compared to pasture groups.
Article
Biochemistry & Molecular Biology
Fernando Jose Schalch Junior, Guilherme Henrique Gebim Polizel, Fernando Augusto Correia Queiroz Cancado, Aricia Christofaro Fernandes, Isabela Mortari, Pedro Ratto Lisboa Pires, Heidge Fukumasu, Miguel Henrique de Almeida Santana, Arlindo Saran Netto
Summary: This study investigated the impact of different prenatal nutrition on the plasma metabolome of Nellore dams and their offspring. The findings suggest that fetal programming mainly affects amino acid metabolism and may result in transgenerational epigenetic changes.
Article
Biochemistry & Molecular Biology
Andressa de Zawadzki, Maja Thiele, Tommi Suvitaival, Asger Wretlind, Min Kim, Mina Ali, Annette F. Bjerre, Karin Stahr, Ismo Mattila, Torben Hansen, Aleksander Krag, Cristina Legido-Quigley
Summary: Feces, the byproduct of our diet, has been linked to gut diseases and metabolic disorders. To analyze metabolites in fecal samples efficiently, we developed a novel sample preparation method that allows for the detection of both polar and non-polar metabolites. By using this method, we analyzed 475 fecal samples from patients with liver disease and healthy controls, and found reproducibility and coverage of 28 metabolites.
Article
Medicine, General & Internal
Ninna H. Tougaard, Marie Frimodt-Moller, Hanne Salmenkari, Elisabeth B. Stougaard, Andressa D. Zawadzki, Ismo M. Mattila, Tine W. Hansen, Cristina Legido-Quigley, Sohvi Horkko, Carol Forsblom, Per-Henrik Groop, Markku Lehto, Peter Rossing
Summary: This study investigated the effects of butyrate on inflammation, kidney function, metabolites, and gastrointestinal symptoms in individuals with type 1 diabetes, albuminuria, and intestinal inflammation. The results showed that 12 weeks of butyrate supplementation did not reduce intestinal inflammation in these individuals.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Agriculture, Dairy & Animal Science
Arlindo Saran Netto, Thiago H. Silva, Mellory M. Martins, Ana Maria C. Vidal, Marcia S. Salles, Luiz C. Roma Junior, Marcus A. Zanetti
Summary: Feeding sunflower oil, selenium, and vitamin E to lactating dairy cows improves the nutritional profile of milk and positively impacts animal performance, benefiting both human and animal health.
Article
Biochemistry & Molecular Biology
Gabriela de Pauli Meciano, Fernando Jose Schalch Junior, Guilherme Henrique Gebim Polizel, Aricia Christofaro Fernandes, Roberta Cavalcante Cracco, Arlindo Saran Netto, Rodrigo da Costa Gomes, Nara Regina Brandao Consolo, Miguel Henrique de Almeida Santana
Summary: This study evaluated the effects of gestational supplementation strategy on nitrogen isotopic signature in the blood plasma of beef cows and their progeny. The results showed that different supplementation strategies can influence the nutritional metabolism and isotopic fractionation of cows and calves after birth.
Article
Agriculture, Dairy & Animal Science
Thiago Kan Nishimura, Amanda Guimaraes da Silva, Gabriela Abitante, Carl Robertson Dahlen, Rodrigo Silva Goulart, German Dario Ramirez Zamudio, Saulo Luz Silva, Miguel Henrique de Almeida Santana, Arlindo Saran Netto, Paulo Roberto Leme, Guilherme Pugliesi
Summary: This study evaluated the effects of early weaning on body composition, hormone concentrations, metabolites, and reproductive performance of Nelore cows. The results showed that early weaning at 150 days can successfully recover the cow's body condition and improve the pregnancy success rate in the next breeding season, regardless of the cow's parity order.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Physics, Multidisciplinary
S. Athira, Saulo L. L. Silva, Probal Nag, Sushma Lakshmi, C. Sharath Kumar, Debendra Prasad Panda, Sayan Das, Sarita Rajput, Andrews P. Alex, A. Sundaresan, Sivaranjana Reddy Vennapusa, T. Maitra, D. Jaiswal-Nagar
Summary: In this paper, the authors used the distance between the states as a theoretical measure to determine the bipartite entanglement in a one-dimensional magnetic dimer system. The calculation was compared with the concurrence, a well-known entanglement measure, and found to be the same. Experimental verification was done on single crystals of copper acetate, showing the existence of bipartite entanglement even at room temperature. Characterization and calculations revealed the weak intermolecular interactions and low probability of direct Cu-Cu exchange in copper acetate.
NEW JOURNAL OF PHYSICS
(2023)
Article
Agriculture, Dairy & Animal Science
Renata Maria Consentino Conti, Thiago Henrique da Silva, Iuli Caetanoda Silva Brandao Guimaraes, Helena Viel Alves Bezerra, Arlindo Saran Netto, Paulo Henrique Mazza Rodrigues, Jose Carlos Guilardi Pacheco, Marcus Antonio Zanetti
Summary: This study aimed to investigate the effects of molybdenum, organic and inorganic sources of copper and sulfur on lamb performance, carcass traits, and blood concentration of these minerals. The results showed that these sources did not improve performance or carcass traits. However, molybdenum and sulfur had a negative impact on copper bioavailability and metabolism, while organic sources of copper and sulfur increased blood concentrations of these minerals.
Article
Agriculture, Dairy & Animal Science
Larissa S. Gheller, Mellory M. Martins, Thiago H. Silva, Gustavo Freu, Marcia S. V. Salles, Luiz C. R. Junior, Weber V. B. Soares, Arlindo S. Netto
Summary: This study evaluated the effects of feeding an ultra-diluted complex to dairy cows during the transition period and early lactation. The results showed that the ultra-diluted complex had no direct impact on the cows' dry matter intake, but the cows in the treatment group had a higher dry matter intake relative to their body weight. These cows also showed a trend towards lower somatic cell counts, indicating better udder health, and improved liver function. These findings suggest that the use of ultra-diluted complexes may be beneficial for dairy cows during the transition period and early lactation.
Article
Veterinary Sciences
Vicente Luiz Macedo Buarque, Helena Viel Alves Bezerra, Guilherme Pegoraro Rissi, Regner italo Goncalves de Oliveira, Natalia Marques da Silva, Nara Regina Brandao Consolo, German Dario Ramirez-Zamudio, Ricardo de Francisco Strefezzi, Sarita Bonagurio Gallo, Saulo Luz Silva, Paulo Roberto Leme
Summary: This study evaluates the effectiveness of different additives in preventing urolithiasis in lambs fed a high-concentrate diet. The results show that the additives do not affect the lambs' performance and health, and although they acidify urine, they do not completely prevent the formation of urolithiasis.
VETERINARY SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Patricia Maloso Ramos, Priscila Robertina dos Santos-Donado, Giuliana Micai de Oliveira, Carmen Josefina Contreras-Castillo, Tracy L. Scheffler, Saulo da Luz e Silva, Luciane Silva Martello, Eduardo Francisquine Delgado
Summary: The temperament of Bos taurus indicus affects beef tenderization. This study investigated the temperament and performance of non-castrated Nellore cattle and identified two groups based on Longissimus lumborum (LL) pH decline, with the pH-Res group showing lower beef quality. However, the occurrence of LL pH between 5.8 and 5.9 did not affect tenderization rate or extension but did impact water holding capacity.
