Journal
MICROORGANISMS
Volume 8, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms8030351
Keywords
antibacterial activity; Ralstonia solanacearum; Streptomyces sp. NEAU-HV9; actinomycin D
Categories
Funding
- National Natural Youth Science Foundation of China [31701858]
- China Postdoctoral Science Foundation [2018M631907]
- Heilongjiang Postdoctoral Fund [LBH-Z17015]
- University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province [UNPYSCT-2017017]
- Young Talents Project of Northeast Agricultural University [17QC14]
Ask authors/readers for more resources
Ralstonia solanacearum is an important soil-borne bacterial plant pathogen. In this study, an actinomycete strain named NEAU-HV9 that showed strong antibacterial activity against Ralstonia solanacearum was isolated from soil using an in vitro screening technique. Based on physiological and morphological characteristics and 98.90% of 16S rRNA gene sequence similarity with Streptomyces panaciradicis 1MR-8T, the strain was identified as a member of the genus Streptomyces. Tomato seedling and pot culture experiments showed that after pre-inoculation with the strain NEAU-HV9, the disease occurrence of tomato seedlings was effectively prevented for R. solanacearum. Then, a bioactivity-guided approach was employed to isolate and determine the chemical identity of bioactive constituents with antibacterial activity from strain NEAU-HV9. The structure of the antibacterial metabolite was determined as actinomycin D on the basis of extensive spectroscopic analysis. To our knowledge, this is the first report that actinomycin D has strong antibacterial activity against R. solanacearum with a MIC (minimum inhibitory concentration) of 0.6 mg L-1 (0.48 mu mol L-1). The in vivo antibacterial activity experiment showed that actinomycin D possessed significant preventive efficacy against R. solanacearum in tomato seedlings. Thus, strain NEAU-HV9 could be used as BCA (biological control agent) against R. solanacearum, and actinomycin D might be a promising candidate for a new antibacterial agent against R. solanacearum.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available