Article
Neurosciences
Antonio Cibelli, Sandra Veronica Lopez-Quintero, Sean Mccutcheon, Eliana Scemes, David C. Spray, Randy F. Stout, Sylvia O. Suadicani, Mia M. Thi, Marcia Urban-Maldonado
Summary: The study successfully transduced mouse cortical astrocytes using a lentiviral vector encoding hTERT, generating immortalized cell lines with characteristics consistent with neonatal astrocytes. These cell lines showed stability in the expression of astrocyte-specific markers over cell passages.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Xiaoyu Liu, Mingru Bai, Yimin Sun, Xuchen Hu, Chenglin Wang, Jing Xie, Ling Ye
Summary: In osteoblasts, FGF7 promotes the expression of E11, leading to dendrites elongation and the formation of functional gap junctions through connexin43 (Cx43) interaction. This process is mediated by the MAPK pathway and PI3K-AKT pathway, suggesting a significant role of FGF7 in later stages of bone development and homeostasis.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jin Kono, Masakatsu Ueda, Atsushi Sengiku, Sylvia O. Suadicani, Osamu Ogawa, Hiromitsu Negoro
Summary: The study suggests that urothelial Cx43 mediated ATP signaling and coordination of urothelial activity are essential for proper perception and regulation of responses to bladder distension in the animals' awake, active phase. This research highlights the significant role of Cx43 in bladder function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Zuping Wu, Chenchen Zhou, Quan Yuan, Demao Zhang, Jing Xie, Shujuan Zou
Summary: CTGF promotes intercellular communication in chondrocytes through upregulating Cx43 expression and activating the PI3K/Akt signaling pathway.
CELL PROLIFERATION
(2021)
Article
Biology
Mingyang Huang, Anying Song, Xi Chen, Sarah Ishtiaq, Chunmei Wang, Darryl L. Hadsell, Qiong A. Wang, Yi Zhu
Summary: Breastfeeding offers health benefits for both mothers and infants, but the increase in obesity in the U.S. has made it difficult for many mothers to produce and express breastmilk. The quality of breastmilk from obese mothers is also compromised. This study investigates the role of Connexin43 in adipocyte remodeling and breastmilk production, suggesting that enhancing adipocyte Connexin43 may help obese mothers produce better breastmilk to support their neonates' growth.
Article
Cell Biology
Jing-Jun Zhao, Zheng-Hui Wang, Ying-Jie Zhang, Wen-Jing Wang, Ai-Fang Cheng, Pei-Jing Rong, Chun-Lei Shan
Summary: This study investigated the impact of auricular vagus nerve stimulation on the recovery of locomotor function in rats with cerebral ischemia/reperfusion injury. The findings suggested that the stimulation can promote recovery by altering the secretion of acetylcholine and inflammatory factors, as well as the phosphorylation of connexin 43.
NEURAL REGENERATION RESEARCH
(2022)
Article
Reproductive Biology
Lin Yin, Wusu Wang, Weijun Pang, Gongshe Yang, Lei Gao, Guiyan Chu
Summary: Gap junction intercellular communication plays an important role in follicular development. In porcine follicles, Cx43 is the most widely expressed connexin. Insulin can enhance Cx43 protein expression, which is dependent on the phosphorylated activities of AKT and Erk.
Article
Biochemistry & Molecular Biology
Olivia E. Crouthamel, Leping Li, Michael T. Dilluvio, Thomas W. White
Summary: Mutations in the GJA1 gene encoding connexin43 (Cx43) have been linked to various rare genetic disorders, including those affecting the skin. This study investigated the functional consequences of a specific Cx43 mutation (Cx43-G38E) associated with a novel human phenotype. The research showed that the mutation increased the activity of hemichannels, suggesting a potential role in skin and skeletal disorders caused by Cx43 mutations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Zhifeng Qi, Shuhua Yuan, Ke Jian Liu, Xunming Ji
Summary: This study found that normobaric hyperoxia treatment could reduce oxidative stress and maintain the level of connexin43 in astrocytes, showing neuroprotective effects. This discovery provides new insights for the clinical treatment of ischemic stroke.
CNS NEUROSCIENCE & THERAPEUTICS
(2022)
Article
Biology
Yi Zhu
Summary: Connexin43 is a crucial protein in the family that not only plays a critical role in gap junctions but also has various functions independent of gap junctions. It forms different-sized polypeptides through distinct mechanisms and functions in different cellular compartments. Research on Connexin43 will enhance our understanding of how genes increase their functions and may guide the development of therapies for metabolic conditions, cancer, and other diseases.
Article
Biochemistry & Molecular Biology
Kristina Rode, Marion Langeheine, Bettina Seeger, Ralph Brehm
Summary: Studies have shown that Cx43 in spermatogonia/spermatids is not essential for successful termination of spermatogenesis, and using a GC-specific Cx43 KO does not affect fertility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Erin M. O'Shaughnessy, William Duffy, Laura Garcia-Vega, Keith Hussey, A. David Burden, Mozheh Zamiri, Patricia E. Martin
Summary: Elevated expression of Connexin26 in psoriatic tissue may contribute to cellular events imbalance, while inhibition of Connexin signaling reduces pro-inflammatory events and may have therapeutic benefits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Yi Zhu, Na Li, Mingyang Huang, Xi Chen, Yu A. An, Jianping Li, Shangang Zhao, Jan-Bernd Funcke, Jianhong Cao, Zhenyan He, Qingzhang Zhu, Zhuzhen Zhang, Zhao Wang, Lin Xu, Kevin W. Williams, Chien Li, Kevin Grove, Philipp E. Scherer
Summary: Adipose tissue is a potential target for treating obesity and metabolic diseases. By connecting adipocytes via connexin43 gap junctions, the efficacy of pharmacological agents can be improved. This finding suggests that the mechanism of connecting adipocytes can enhance the effectiveness of drugs targeting adipose tissue.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Biochemistry & Molecular Biology
Anna Volnova, Vassiliy Tsytsarev, Olga Ganina, Grace E. Velez-Crespo, Janaina M. Alves, Alla Ignashchenkova, Mikhail Inyushin
Summary: Gap junctions between astrocytes play a crucial role in regulating epileptic seizures by controlling the synchronization of pathological neuronal activity. Blocking these intercellular channels could be an important mechanism in suppressing epileptic activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Rui Dong, Pin Lv, Yuqiang Han, Linhao Jiang, Zimo Wang, Liangyu Peng, Zhengliang Ma, Tianjiao Xia, Bing Zhang, Xiaoping Gu
Summary: This study found that reconfiguring the astrocytic network by increasing the expression of gap junction protein 43 (GJs-Cx43) can improve cognitive dysfunction induced by long-term isoflurane anesthesia. This improvement may be associated with the regulation of oxidative stress and neuroinflammation.
CNS NEUROSCIENCE & THERAPEUTICS
(2022)
Article
Plant Sciences
Jacob P. Walsh, Natasha DesRochers, Justin B. Renaud, Keith A. Seifert, Ken K-C Yeung, Mark W. Sumarah
Summary: This study identified a siderophore produced by Ilyonectria for the first time, linking it to disease virulence. Siderophore production was suppressed as iron concentration increased, providing new insights into ginseng root rot research.
JOURNAL OF GINSENG RESEARCH
(2021)
Article
Chemistry, Analytical
William Pinsky, Aaron Harris, Austyn D. Roseborough, Wenxuan Wang, Ali R. Khan, Kristina Jurcic, Ken K-C Yeung, Stephen H. Pasternak, Shawn N. Whitehead
Summary: Periventricular white matter hyperintensities (pvWMHs) detected by MRI are associated with increased risk of stroke and dementia. MALDI IMS was used to analyze lipid distribution in formalin-fixed human brain tissues, revealing lipid expression differences between pvWMHs and normal white matter.
ANALYTICAL CHEMISTRY
(2021)
Article
Entomology
Ian M. Scott, Gabrielle Hatten, Yazel Tuncer, Victoria C. Clarke, Kristina Jurcic, Ken K. -C. Yeung
Summary: Surveillance and determination of pesticide resistance mechanisms are essential for resistance management. The study utilized proteomic analysis to identify protein differences in susceptible and resistant strains of Colorado potato beetle (CPB), revealing a potential connection between C-type lectin (CTL) expression and insect immunity response to toxins. The findings suggest a novel approach for pest management through targeting insect immunity pathways and highlight the adaptability of CPB to various challenges including synthetic insecticides.
Article
Biochemical Research Methods
Chaochao Chen, Steven R. Laviolette, Shawn N. Whitehead, Justin B. Renaud, Ken K-C Yeung
Summary: In this study, zinc oxide nanoparticles were investigated as an alternative to titanium dioxide nanoparticles for laser desorption/ionization mass spectrometric imaging of low molecular weight molecules. Zinc oxide nanoparticles dissolved in acidic pH showed similar or better results compared to titanium dioxide nanoparticles in imaging LMWM, with regular acid washes effective in maintaining reproducibility. High-quality images of tissue sections were obtained, predominantly detecting ions as Na+ or K+ adducts in the positive ion mode, with ZnO NP detecting a similar number of LMWM as TiO2 NP.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Clinical Neurology
Austyn D. Roseborough, Berk Rasheed, Youngkyung Jung, Kevin Nishimura, William Pinsky, Kristopher D. Langdon, Robert Hammond, Stephen H. Pasternak, Ali R. Khan, Shawn N. Whitehead
Summary: pvWMH are neuroimaging abnormalities associated with factors such as age, neurodegenerative diseases, and cerebrovascular risk factors. The pathological causes of pvWMH are heterogeneous and may involve factors like small vessel disease, ependymal loss, blood-brain barrier dysfunction, and microgliosis. This study found associations between microvessel stenosis, perivascular spaces, and fibrinogen immunohistochemistry with and without periventricular infarction in pvWMH.
Article
Neurosciences
Alexander Levit, Andrew Gibson, Olivia Hough, Youngkyung Jung, Yuksel Agca, Cansu Agca, Vladimir Hachinski, Brian L. Allman, Shawn N. Whitehead
Summary: The study found that behavioral inflexibility and white matter inflammation in AD accelerate compared to normal aging, and may precede impairments in learning and memory.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Cell Biology
Gareth M. Rurak, Stephanie Simard, Moises Freitas-Andrade, Baptiste Lacoste, Francois Charih, Amanda Van Geel, John Stead, Barbara Woodside, James R. Green, Gianfilippo Coppola, Natalina Salmaso
Summary: This study examines the sex differences and developmental changes in cortical astroglial cells. The results reveal two distinct phenotypes of astroglia during early and late development, while sex differences are mainly reflected in gene expression patterns during development.
Article
Engineering, Environmental
Kang Kang, Griffin Loebsack, Tahereh Sarchami, Naomi B. Klinghoffer, Sadegh Papari, Ken K. -C. Yeung, Franco Berruti
Summary: This study focuses on the co-pyrolysis of different biomasses with red mud and investigates the reaction mechanism and characterization of the produced magnetic adsorbents. The findings show that samples prepared at high temperature exhibit the best performance, and cellulose-rich adsorbents have higher adsorption efficacy.
Article
Food Science & Technology
M. J. Kelman, J. B. Renaud, D. Baines, K. K-C Yeung, J. D. Miller, M. W. Sumarah
Summary: Silage, a key component of animal feed in Canada and northern Europe, can contain toxic fungal metabolites and is susceptible to spoilage by yeasts and fungi. This study analyzed silage samples from Canadian dairy goat and cattle farms and identified multiple fungal species and mycotoxins. The findings highlight the importance of implementing best management practices to minimize fungal contamination and mycotoxin deposition to maintain animal health.
WORLD MYCOTOXIN JOURNAL
(2022)
Article
Neurosciences
Mohammed H. Sarikahya, Samantha Cousineau, Marta De Felice, Kendrick Lee, Karen Kw Wong, Marieka V. DeVuono, Tony Jung, Mar Rodriguez-Ruiz, Tsun Hay Jason Ng, Dana Gummerson, Emma Proud, Daniel B. Hardy, Ken K-C Yeung, Walter Rushlow, Steven R. Laviolette
Summary: Despite increased prevalence of maternal cannabis use, little is understood regarding potential long-term effects of prenatal cannabis exposure (PCE) on neurodevelopmental outcomes. This study found that PCE induces behavioral, neuronal, and molecular alterations in the mesolimbic system in male and female offspring, resembling neuropsychiatric endophenotypes. Moreover, PCE resulted in profound dysregulation of critical fatty acid pathways in the developing brain lipidome.
Article
Neurosciences
Marta De Felice, Chaochao Chen, Mar Rodriguez-Ruiz, Hanna J. Szkudlarek, Michael Lam, Selvi Sert, Shawn N. Whitehead, Ken K-C Yeung, Walter J. Rushlow, Steven R. Laviolette
Summary: This study used an integrative approach to investigate the effects of adolescent THC exposure on the dorsal and ventral regions of the hippocampus. The findings reveal long-lasting memory deficits and anxiety-like behaviors, as well as differential molecular and neuronal abnormalities in these hippocampal regions. Additionally, the study demonstrates dysregulation of GABA and glutamate levels, as well as glutamatergic signaling in the dorsal and ventral regions of the hippocampus.
NEUROPSYCHOPHARMACOLOGY
(2023)
Article
Medicine, Research & Experimental
Marissa A. Lithopoulos, Xavier Toussay, Shumei Zhong, Liqun Xu, Shamimunisa B. Mustafa, Julie Ouellette, Moises Freitas-Andrade, Cesar H. Comin, Hayam A. Bassam, Adam N. Baker, Yiren Sun, Michael Wakem, Alvaro G. Moreira, Cynthia L. Blanco, Arul Vadivel, Catherine Tsilfidis, Steven R. Seidner, Ruth S. Slack, Diane C. Lagace, Jing Wang, Baptiste Lacoste, Bernard Thebaud
Summary: Preterm birth is the leading cause of death in children under 5 years old. Premature infants often develop bronchopulmonary dysplasia (BPD), which can lead to abnormal neurodevelopment. Research in newborn mice showed that hyperoxia-induced BPD resulted in lifelong impairments in cerebrovascular structure and function, as well as impairments in neural progenitor cell self-renewal and neurogenesis. The dysregulation of genes involved in cell proliferation, angiogenesis, vascular autoregulation, neuronal formation, and neurotransmission was also observed in this study. These findings suggest a relationship between BPD and abnormal neurodevelopmental outcomes.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Agriculture, Multidisciplinary
Jacob P. Walsh, Christopher P. Garnham, Ken K-C Yeung, Mark W. Sumarah
Summary: This translated article describes a decrease in crop yields caused by ginseng root rot, and highlights the important role of TAFC in the virulence of the ginseng pathogens. The results demonstrate that enzymatic degradation of TAFC can effectively control ginseng root rot.
ACS AGRICULTURAL SCIENCE & TECHNOLOGY
(2022)
Meeting Abstract
Obstetrics & Gynecology
Kendrick Lee, Mohammed H. Sarikahya, Tony Jung, Samantha L. Cousineau, Ken K. -C. Yeung, Steven R. Laviolette, Daniel B. Hardy
REPRODUCTIVE SCIENCES
(2022)