Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning

MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme

(2018) Da Lin et al.   NUCLEIC ACIDS RESEARCH

Single Day Construction of Multigene Circuits with 3G Assembly

(2018) Andrew D. Halleran et al.   ACS Synthetic Biology

T5 exonuclease-dependent assembly offers a low-cost method for efficient cloning and site-directed mutagenesis

(2018) Yongzhen Xia et al.   NUCLEIC ACIDS RESEARCH

GB3.0: a platform for plant bio-design that connects functional DNA elements with associated biological data

(2017) Marta Vazquez-Vilar et al.   NUCLEIC ACIDS RESEARCH

Twin-primer non-enzymatic DNA assembly: an efficient and accurate multi-part DNA assembly method

(2017) Jing Liang et al.   NUCLEIC ACIDS RESEARCH

Generation of stable infectious clones of plant viruses by using Rhizobium radiobacter for both cloning and inoculation

(2017) Decai Tuo et al.   VIROLOGY

Direct observation of DNA threading in flap endonuclease complexes

(2016) Faizah A AlMalki et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Seamless Insert-Plasmid Assembly at High Efficiency and Low Cost

(2016) Roger M. Benoit et al.   PLoS One

Direct observation of DNA threading in flap endonuclease complexes

(2016) Faizah A AlMalki et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

COLORFUL-Circuit: A Platform for Rapid Multigene Assembly, Delivery, and Expression in Plants

(2016) Hassan Ghareeb et al.   Frontiers in Plant Science

Hot Fusion: An Efficient Method to Clone Multiple DNA Fragments as Well as Inverted Repeats without Ligase

(2015) Changlin Fu et al.   PLoS One

Unique nucleotide sequence–guided assembly of repetitive DNA parts for synthetic biology applications

(2014) Joseph P Torella et al.   Nature Protocols

Significant reduction of BiFC non-specific assembly facilitatesin plantaassessment of heterotrimeric G-protein interactors

(2014) Timothy E. Gookin et al.   PLANT JOURNAL

IRDL Cloning: A One-Tube, Zero-Background, Easy-to-Use, Directional Cloning Method Improves Throughput in Recombinant DNA Preparation

(2014) Jiancai Wang et al.   PLoS One

Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly

(2013) Joseph P. Torella et al.   NUCLEIC ACIDS RESEARCH

One-pot DNA construction for synthetic biology: the Modular Overlap-Directed Assembly with Linkers (MODAL) strategy

(2013) Arturo Casini et al.   NUCLEIC ACIDS RESEARCH

GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis

(2013) Athanasios Lampropoulos et al.   PLoS One

A 2in1 cloning system enables ratiometric bimolecular fluorescence complementation (rBiFC)

(2012) Christopher Grefen et al.   BIOTECHNIQUES

High-Throughput Construction of Intron-Containing Hairpin RNA Vectors for RNAi in Plants

(2012) Pu Yan et al.   PLoS One

A Modular Cloning System for Standardized Assembly of Multigene Constructs

(2011) Ernst Weber et al.   PLoS One

GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules

(2011) Alejandro Sarrion-Perdigones et al.   PLoS One

In-Fusion BioBrick assembly and re-engineering

(2010) Sean C. Sleight et al.   NUCLEIC ACIDS RESEARCH

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

(2009) Carola Engler et al.   PLoS One

Development of a transient expression system in grapevine via agro-infiltration

(2008) M. Santos-Rosa et al.   PLANT CELL REPORTS

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One