Article
Nanoscience & Nanotechnology
Hannah Donnelly, Mark R. Sprott, Anup Poudel, Paul Campsie, Peter Childs, Stuart Reid, Manuel Salmeron-Sanchez, Manus Biggs, Matthew J. Dalby
Summary: There is a clinical demand for viable bone grafts, and tissue engineering strategies using mesenchymal stromal cells (MSCs) to create potential bone-graft materials can help fulfill this need. However, the long culture periods and cost associated with MSC osteogenesis in a clinical setting necessitate the investigation of strategies for optimizing cell production. In this study, a piezoelectric copolymer with a fibronectin network coating is used to enhance MSC adhesion and present growth factors, and electrical stimulation is applied to investigate the MSC response.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Medicine, Research & Experimental
Mengyuan Wang, Ying Li, Hongqiao Wang, Meng Li, Xiaomin Wang, Rongzhen Liu, Daijun Zhang, Wenhua Xu
Summary: Corneal epithelial defects and excessive wound healing can cause severe complications. Stem cells offer a promising solution for regenerating the corneal epithelium and treating severe corneal epithelial injury. Biological scaffolds such as the amniotic membrane, fibrin, and hydrogels provide the necessary signals for stem cell proliferation and differentiation. Researchers have evaluated these scaffolds as potential therapeutic interventions for corneal disorders, identifying both benefits and drawbacks. This study provides a comprehensive overview of the history and use of stem cells in corneal repair, focusing on biological scaffolds used in stem cell transplantation and innovative materials under investigation.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Cell & Tissue Engineering
Dehua Chang, Taibing Fan, Shuang Gao, Yongqiang Jin, Mingkui Zhang, Minoru Ono
Summary: The review discusses the use of MSC sheets for improving cardiac function and alleviating adverse ventricular remodeling in ischemic heart tissue. Among various types of MSCs, UC-MSCs are highlighted as a promising candidate due to their unique features and ease of isolation and culture.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell & Tissue Engineering
Mahboube Bahroudi, Bita Bakhshi, Sara Soudi, Shahin Najar-Peerayeh
Summary: The study demonstrated that LPS-MSC-CM can modulate the immune response to V. cholerae LPS in mice by increasing antibody levels, decreasing inflammatory responses, and inducing protective antibodies against V. cholerae infection.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Engineering, Biomedical
Bin Wang, Wei Liu, Jiao Jiao Li, Senlin Chai, Dan Xing, Hongsheng Yu, Yuanyuan Zhang, Wenjin Yan, Zhihong Xu, Bin Zhao, Yanan Du, Qing Jiang
Summary: In the treatment of osteoarthritis, delivering low doses of MSCs through microcarriers can achieve similar reparative effects as repeated high doses. Mechanistic investigations reveal that 3D growth of MSCs reduces inflammatory factors, enhances early chondrogenesis and extracellular matrix interactions, and maintains the phenotype of co-cultured chondrocytes.
BIOACTIVE MATERIALS
(2022)
Article
Medicine, Research & Experimental
Arezou Khosrojerdi, Sara Soudi, Ahmad Zavaran Hosseini, Sahar Ghaffari Khaligh, Seyed Mahmoud Hashemi
Summary: This study investigated the effect of different treatments on systemic and local inflammation in a mouse model of sepsis. MSC-derived exosomes combined with imipenem controlled inflammation and increased TCD4+ and Treg cell populations. Hepatocyte-derived exosomes combined with imipenem reduced liver inflammation and increased TCD8+ and Treg cell populations. The mixture of exosomes combined with imipenem showed the best function in reducing inflammation, maintaining all T lymphocyte populations, reducing liver damage, and increasing survival rate.
Article
Cell Biology
Denise Zujur, Ziadoon Al-Akashi, Anna Nakamura, Chengzhu Zhao, Kazuma Takahashi, Shizuka Aritomi, William Theoputra, Daisuke Kamiya, Koichi Nakayama, Makoto Ikeya
Summary: We developed a stepwise differentiation method to generate matrix-rich cartilage spheroids from induced pluripotent stem cell-derived mesenchymal stem/stromal cells (iMSCs) via the induction of neural crest cells under xeno-free conditions. By using a thienoindazole derivative, TD-198946, we achieved enhanced chondrogenesis in iMSCs. Our findings provide a novel cell source for stem cell-based cartilage repair and offer the potential for biofabrication of larger cartilage tissues using chondrogenic spheroids and technologies such as Kenzan Bioprinting.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Thang Quoc Bui, Nguyen Trong Binh, Truc Le-Buu Pham, Trinh Le Van, Nhung Hai Truong, Dang Phu-Hai Nguyen, Thao Thi-Thu Luu, Trang Nguyen-Xuan Pham, Tu Cam Tran, Huyen Thuong-Thi Nguyen, Nhu Thuy-Trinh, Phong Anh Tran
Summary: In this study, the transplantation of human umbilical cord-derived mesenchymal stem cell (MSC) sheets was investigated as a potential treatment for myocardial infarction (MI). The findings showed that MSC sheets with higher cell density had better therapeutic effects on MI mice, with significant improvements in weight gain and ejection fraction recovery. In conclusion, hUC-MSC sheets ameliorated heart MI injury in mice and their efficacy improved with increased cell density.
Review
Immunology
Seyyed Mohammad Matin Alavi-Dana, Yazdan Gholami, Mohammadreza Meghdadi, Mohammad Saleh Fadaei, Vahid Reza Askari
Summary: This review discusses the pathogenesis of COVID-19 infection and the role of MSCs in suppressing inflammation and cytokine storm. Additionally, it reviews clinical trials and systematic reviews on the safety and efficacy of MSC therapy in the treatment of COVID-19 infection.
INFLAMMOPHARMACOLOGY
(2023)
Article
Cell & Tissue Engineering
Naoto Honda, Yasuhiro Watanabe, Yuta Tokuoka, Ritsuko Hanajima
Summary: This study investigated the roles of microglia, macrophages, and acquired antibodies in cell sheet transplantation in a mouse model. In vitro experiments showed that MSCs induced M2 polarization in macrophages and promoted their migration. Despite the favorable characteristics of microglia and macrophages, their elimination improved the survival of donor cells in vivo.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biotechnology & Applied Microbiology
Yun-Feng Zhou, Di Zhang, Wan-Ting Yan, Kai Lian, Zheng-Zheng Zhang
Summary: This article reviews the therapeutic potential of multipotent stromal cells (MSCs) in repairing meniscal injuries and discusses the avenues for utilizing these cells. The current progress on biomaterial implants is also reviewed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Materials Science, Biomaterials
Yongtao Wang, Nana Wang, Yazhou Chen, Yingjun Yang
Summary: In this study, micropattern-induced interfacial heterogeneity was created in breast cancer cells to regulate the distribution of focal adhesions and cytoskeleton in the central and peripheral regions of cell clusters. The heterogeneity of the interfacial properties induced biased tension force, promoting the development of a stem-like phenotype.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Biotechnology & Applied Microbiology
Lu Lu, Lingli Quan, Jian Li, Junbin Yuan, Xinmin Nie, Xueyuan Huang, Hang Dong, Yanrong Su, Yufen Huang, Qingjie Kou, Leping Liu, Haiting Liu, Xionghui Zhou, Rong Gui, Lan Gu
Summary: A novel nanometer drug loading system, FZ/MER-AgMOF@Bm, was successfully constructed for sepsis management. The system effectively alleviated excessive inflammatory response and eliminated bacteria, while also promoting the polarization of macrophages to M2 and reducing lung injury. The nanoparticles could be a potential new strategy for sepsis management by combining anti-inflammatory and antimicrobial properties.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Punya Sachdeva, Seongmin Ji, Shampa Ghosh, Soumya Ghosh, Manchala Raghunath, Hyunjin Kim, Rakesh Bhaskar, Jitendra Kumar Sinha, Sung Soo Han
Summary: Major Depressive Disorder (MDD), also known as depression, affects approximately 3.8% of the global population, with 5.0% being adults and 5.7% being above the age of 60. MDD is characterized by subtle alterations in gray and white matter, impacting various brain regions. It can have a detrimental impact on overall health and can significantly impair personal, professional, and social functioning. Antidepressants are commonly used to manage clinical depression by modulating neurotransmitter levels, but a significant percentage of patients do not respond adequately. Recent research suggests that stem cells may hold promise in understanding and treating depression.
Article
Cell & Tissue Engineering
Vincent P. Willard, Holly A. Leddy, Daniel Palmer, Chia-Lung Wu, Wolfgang Liedtke, Farshid Guilak
Summary: The expression and activation of TRPV4 in chondrogenesis play a crucial role in regulating cartilaginous matrix production through modulation of chondrogenic gene markers and Ca2+ signaling. Understanding the involvement of TRPV4 in cartilage development may lead to new therapeutic strategies for cartilage-related diseases and accelerate stem cell differentiation for tissue engineering purposes.
Review
Chemistry, Analytical
Yutaro Maekawa, Eri Ayano, Kenichi Nagase, Hideko Kanazawa
Summary: The focus of drug discovery and therapeutics has shifted to biopharmaceuticals and regenerative medicine, requiring the development of new technologies. A temperature-responsive separation system based on modified polymers has been developed as an effective tool for protein and cell separation applications.
ANALYTICAL SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Kenta Homma, Yuji Ohta, Kosuke Minami, Genki Yoshikawa, Kenichi Nagase, Aya M. Akimoto, Ryo Yoshida
Summary: The study found that graft density of the self-oscillating polymer brush is crucial for mechanical oscillation, affecting equilibrium swelling and thermo-responsive behavior. Adequate graft density enables autonomous mechanical oscillation, with significantly improved amplitude and period compared to previous modification methods.
Article
Multidisciplinary Sciences
Kenichi Nagase, Yuta Umemoto, Hideko Kanazawa
Summary: Temperature-responsive chromatography using thermoresponsive polymers can control analyte retention via column temperature. In this study, the effect of pore diameter of silica beads on analyte elution behavior was examined, and it was found that bead pore diameter determines temperature-dependent elution behavior.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Physical
Kenichi Nagase
Summary: PNIPAAm is a well-known stimuli-responsive polymer in the biomedical field due to its ability to undergo hydration and dehydration reactions with temperature variations, leading to interactions with biomolecules, proteins, and cells. The temperature-dependent property of PNIPAAm makes it functional for various biomedical applications, particularly in bioseparation and tissue engineering.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Analytical
Kenichi Nagase, Yuta Ishizawa, Masakazu Inoue, Matsurika Kokubun, Sota Yamada, Hideko Kanazawa
Summary: The temperature-responsive spin column presents a simple sample preparation method using all-aqueous eluent for protein removal from serum, required for serum drug analysis. Two types of silica beads grafted with temperature-responsive copolymers were utilized as packing materials, enabling effective separation of serum proteins and analytes by changing the temperature during elution.
ANALYTICA CHIMICA ACTA
(2021)
Article
Multidisciplinary Sciences
Kenichi Nagase, So Inoue, Masakazu Inoue, Hideko Kanazawa
Summary: In this study, a new two-dimensional chromatography system using PNIPAAm-modified silica beads was developed for effective and safe drug monitoring. The system separates serum proteins and drugs by adjusting the temperature and uses all-aqueous mobile phases.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Chemical
Kenichi Nagase, Sakiko Kitazawa, Toshihiko Kogure, Sota Yamada, Kazuhiro Katayama, Hideko Kanazawa
Summary: A temperature-modulated viral vector purification column using a mixed polymer brush as packing material was developed in this study. The column successfully eluted the AAV2 vector and separated it from bovine serum proteins.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Kenichi Nagase, Kosuke Matsumoto, Hideko Kanazawa
Summary: In this study, mixed-mode chromatography columns were developed using PNIPAAm and PAPTAC brush-modified silica beads and evaluated on various analytes. The results showed that the columns could effectively separate different compounds through modulating hydrophobic and electrostatic interactions.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Kenichi Nagase, Hikaru Takagi, Hideo Nakada, Haruki Ishikawa, Yoshiko Nagata, Tohru Aomori, Hideko Kanazawa
Summary: Therapeutic drug monitoring is critical in pharmacological therapy. In this study, thermoresponsive chromatography columns with various cationic properties were developed for effective drug monitoring.
SCIENTIFIC REPORTS
(2022)
Article
Biophysics
Kenichi Nagase, Akari Okada, Junnosuke Matsuda, Daiju Ichikawa, Yutaka Hattori, Hideko Kanazawa
Summary: In this study, a thermoresponsive cationic block copolymer brush-grafted beads were developed as packing materials for ADSC separation columns. These columns can adsorb ADSCs at 37 degrees C and elute them at 4 degrees C, while other cells are not adsorbed. This separation column can be used to isolate ADSCs without cell surface modification, while maintaining cell activity.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Physical
Hironobu Takahashi, Haruno Wakayama, Kenichi Nagase, Tatsuya Shimizu
Summary: This study presents a multilayering approach to produce engineered human muscle tissue with sufficient contractile force. The tissue shows unidirectional contraction and can quantitatively measure drug effects.
Article
Engineering, Biomedical
Kenichi Nagase, Kaichi Yamazaki, Yutaro Maekawa, Hideko Kanazawa
Summary: Temperature-modulated exosome-capturing materials were developed using thermoresponsive polymers and peptides with affinity for exosomes. The copolymer-grafted silica beads successfully captured and released targeted exosomes via temperature modulation.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Biomedical
Kenichi Nagase, Haruno Wakayama, Junnosuke Matsuda, Naoto Kojima, Hideko Kanazawa
Summary: Thermoresponsive mixed polymer brushes were developed to modulate cell adhesion through temperature change. These brushes enhanced cell adhesion and exhibited thermoresponsive adhesion and detachment properties with different cell types.
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Physical
Kenichi Nagase, Junnosuke Matsuda, Ayako Takeuchi, Yuka Ikemoto
Summary: The hydration and dehydration behaviors of PNIPAAm-grafted silica beads were analyzed using FTIR spectroscopy. The results showed that the peak intensity and position of the FTIR spectra varied with different relative humidity and temperature conditions, indicating the strong hydration and dehydration properties of PNIPAAm.
SURFACES AND INTERFACES
(2023)
Article
Materials Science, Biomaterials
Kenichi Nagase, Naoto Kojima, Mitsuaki Goto, Toshihiro Akaike, Hideko Kanazawa
Summary: In this study, a hepatocyte cell separation material was developed using a thermoresponsive polymer and a polymer with high affinity to hepatocytes. The temperature modulation allowed for the effective separation of hepatocytes from other cells. This material holds significance for cell therapy and liver tissue engineering.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Cell & Tissue Engineering
Jinghui Xu, Zhenxiao Ren, Tianzuo Niu, Siyuan Li
Summary: This study discovers that MSCs-EVs attenuate inflammation and oxidative stress in spinal cord injury (SCI) by delivering miR-26b-5p to target the KDM6A/NOX4 axis, facilitating recovery.
REGENERATIVE THERAPY
(2024)
Article
Cell & Tissue Engineering
Shant Nepal, Jinyan Si, Shohei Ishikawa, Masaki Nishikawa, Yasuyuki Sakai, Aya M. Akimoto, Hiroyuki Okada, Shinsuke Ohba, Ung-il Chung, Takamasa Sakai, Hironori Hojo
Summary: In this study, we evaluated the potential of oligo-tetra-PEG gel (Oligo gel) as a growth factor-releasing scaffold. Compared to tetra-PEG hydrogel (Tetra gel), Oligo gel underwent a higher degree of gel-gel phase separation, demonstrating better protein release and osteogenic potential, and achieving superior regeneration in a mouse calvarial defect model. Therefore, Oligo gel is considered a promising therapeutic biomaterial.
REGENERATIVE THERAPY
(2024)
Article
Cell & Tissue Engineering
Yasuhiro Homma, Sayuri Uchino, Masashi Nagao, Takanori Wakayama, Shin Fukusato, Tomonori Baba, Taiji Watari, Koju Hayashi, Yoshitomo Saita, Muneaki Ishijima
Summary: This study investigates the safety and feasibility of locoregional PRP injection for iliopsoas impingement after THA, and finds that it is both safe and feasible.
REGENERATIVE THERAPY
(2024)
Article
Cell & Tissue Engineering
Taichi Tenkumo, Benedikt Kruse, Kathrin Kostka, Viktoriya Sokolova, Toru Ogawa, Nobuhiro Yoda, Oleg Prymak, Osamu Suzuki, Keiichi Sasaki, Matthias Epple
Summary: This study evaluated the bone-healing effects of a triple-functionalized CaP paste in the rat femoral head. The results showed that the paste significantly accelerated bone healing after 21 days and increased the levels of bone formation-related markers at the protein and gene levels.
REGENERATIVE THERAPY
(2024)
