Journal
CARBOHYDRATE POLYMERS
Volume 150, Issue -, Pages 308-318Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.05.015
Keywords
Pectic galactan; Quaternized pectic galactan; CHPTAC; 5-DTAF; C6 rat glioma cells; Cell specific carrier
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Funding
- Binational Science Foundation (BSF, United States-Israel) [2009178]
- Focal Technological Area (FTA) Program of the Israel National Nanotechnology Initiative (INNI)
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Quaternized derivatives of pectic galactan (QPG) were synthesized by a reaction of pectic galactan (PG) with 3-chloro-2-hydroxypropyl trimethyl ammonium chloride (CHPTAC) in the presence of aqueous sodium hydroxide solution under mild reaction conditions. The results showed that the concentration of CHPTAC and NaOH has great impact on the quaternization reaction. QPG was found to interact electrostatically with plasmid DNA in aqueous solution to form complexes in globular condensed morphology in a nanometer scale size ranging from 60 to 160nm. Complexes formed with QPG fluorescently labeled with 5-DTAF (QPG-5-DTAF) were introduced to the C6 rat glioma cell line, and were found to be able to enter the cell and approach the nucleus within 24 h. The results suggest that this type of modified natural polysaccharide may have an advantage as a biocompatible and biodegradable gene delivery carrier and furthermore may serve as a cell specific carrier. (C) 2016 Elsevier Ltd. All rights reserved.
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