Journal
CARBOHYDRATE POLYMERS
Volume 143, Issue -, Pages 288-295Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.02.032
Keywords
Low-molecular-weight chitosan; Gadolinium (III) complex; Dual-modal imaging; Magnetic resonance (MR) imaging; Fluorescence; Contrast agents
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Funding
- National Natural Science Foundation of China [21471069/21261008/21302071]
- Natural Science Foundation of the Jiangsu Higher Education Institutions of China [14KJB150006]
- Jiangsu Planned Projects for Postdoctoral Research Funds [1401050C]
- Scientific Research Foundation for Advanced Talents of Jiangsu University [14JDG054]
- Scientific Research Foundation for undergraduate of Jiangsu University [14A034]
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The fusion of molecular and anatomical modalities facilitates more reliable and accurate detection in clinic. In this work, we prepared gadolinium (III) complex Gd-DTPA-FITC-CS11 with magnetic resonance (MR) and fluorescence dual-modal imaging modalities. Gd-DTPA-FITC-CS11 consisted of fluorescein isothiocyanate and low-molecular-weight chitosan (CS11) units conjugated with gadolinium diethyle-netriamine pentaacetic acid (Gd-DTPA). Gd-DTPA-FITC-CS11 exhibited a higher longitudinal relaxivity (14.09 mM(-1) s(-1)) than the clinical Gd-DTPA (3.85 mM(-1) s(-1)). T-1-weighted MR contrast enhancement was also demonstrated the comparability to Gd-DTPA at lower dosage. The binding with bovine serum albumin (BSA) was investigated. The fluorescence of BSA in the presence of Gd-DTPA-FITC-CS11 was weakened due to static quenching mechanism. The conformation of BSA was slightly changed but alpha-helix was dominant. The binding was entropy-driven and spontaneous and the main contribution was hydrophobic interaction. Our results suggested the potential of Gd-DTPA-FITC-CS11 as an MR/fluorescence dual-modal imaging contrast agent in improving the diagnostic sensitivity and accuracy. (C) 2016 Elsevier Ltd. All rights reserved.
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