Journal
CELLS
Volume 9, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/cells9020329
Keywords
mouse embryonic stem cell; differentiation protocol; ureteric bud progenitor cells; 3D kidney organoids
Categories
Funding
- H2020 Marie Sklodowska-Curie Actions Innovative Training Network RENALTRACT [642937]
- Academy of Finland [315030, 251314]
- Sigrid Juselius Foundation
- Cancer Research Foundation
- Finnish Cultural Foundation
- Academy of Finland (AKA) [251314, 315030, 251314, 315030] Funding Source: Academy of Finland (AKA)
- Marie Curie Actions (MSCA) [642937] Funding Source: Marie Curie Actions (MSCA)
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Generation of kidney organoids from pluripotent stem cells (PSCs) is regarded as a potentially powerful way to study kidney development, disease, and regeneration. Direct differentiation of PSCs towards renal lineages is well studied; however, most of the studies relate to generation of nephron progenitor population from PSCs. Until now, differentiation of PSCs into ureteric bud (UB) progenitor cells has had limited success. Here, we describe a simple, efficient, and reproducible protocol to direct differentiation of mouse embryonic stem cells (mESCs) into UB progenitor cells. The mESC-derived UB cells were able to induce nephrogenesis when co-cultured with primary metanephric mesenchyme (pMM). In generated kidney organoids, the embryonic pMM developed nephron structures, and the mESC-derived UB cells formed numerous collecting ducts connected with the nephron tubules. Altogether, our study established an uncomplicated and reproducible platform to generate ureteric bud progenitors from mouse embryonic stem cells.
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