Article
Biochemistry & Molecular Biology
Lui Ng, Ryan Wai-Yan Sin, David Him Cheung, Wai-Keung Leung, Abraham Tak-Ka Man, Oswens Siu-Hung Lo, Wai-Lun Law, Dominic Chi-Chung Foo
Summary: Non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease that is increasing worldwide, and it is associated with colorectal polyps. This study aimed to investigate the potential of serum microRNAs (miRNAs) in identifying NAFLD in colorectal polyp patients. The results showed that a serum miRNA panel composed of four miRNA pairs significantly identified NAFLD in colorectal polyp patients, and its performance improved when patients with other concurrent metabolic disorders were removed from the analysis. The serum miRNA panel could be a potential diagnostic biomarker for screening NAFLD in colorectal polyp patients for early diagnosis and prevention of disease progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Edwina Brennan, Alexandra E. Butler, Daniel S. Drage, Thozhukat Sathyapalan, Stephen L. Atkin
Summary: This study found a correlation between PCBs and certain circulating miRNAs, but not with metabolic parameters. In the healthy control group, these miRNAs were associated with menstrual cycle factors, while the correlation was weaker in the PCOS group. This suggests that PCBs may affect fertility through their impact on the hypothalamo-ovarian axis.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Cell Biology
Joanna Patrycja Wroblewska, Michal Stefan Lach, Marcin Rucinski, Igor Piotrowski, Lukasz Galus, Wiktoria Maria Suchorska, Stephanie Kreis, Andrzej Marszalek
Summary: The study found that miRNAs extracted from exosomes of UM patients could be potential biomarkers for diagnosis and metastatic spread of UM.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, General & Internal
Rodolfo Mastropasqua, Rossella D'Aloisio, Erica Costantini, Annamaria Porreca, Giada Ferro, Daniele Libertini, Marcella Reale, Marta Di Nicola, Pasquale Viggiano, Gennaro Falconio, Lisa Toto
Summary: The study evaluated the expression levels of different miRNAs in diabetic patients and found significant differences in expression levels between diabetic patients with and without DR. Significant associations between miRNAs and anatomical/perfusion parameters and functional parameters were observed in the diabetic groups.
Review
Oncology
Tim Nestler, Justine Schoch, Gazanfer Belge, Klaus-Peter Dieckmann
Summary: miR371 is a more sensitive and specific biomarker for testicular germ cell cancer than conventional serum markers. It shows potential for initial diagnosis, treatment monitoring, and follow-up. However, miR371 is not expressed in teratoma. Further studies are needed to determine its importance in detecting testicular tumor recurrences and residual tumors after chemotherapy.
Article
Immunology
Steven D. Hicks, Alexandra Confair, Kaitlyn Warren, Desirae Chandran
Summary: Emerging evidence suggests that non-coding RNAs in maternal breast milk have unique effects on the metabolism and immune system of infants. This study provides the largest dataset of non-coding RNAs in breast milk and demonstrates a relationship between microRNAs in breast milk and maternal diet.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Clinical Neurology
Dorota Wyczechowska, Paul G. Harch, Shelly Mullenix, Erin S. Fannin, Brenda B. Chiappinelli, Duane Jeansonne, Adam Lassak, Nicolas G. Bazan, Francesca Peruzzi
Summary: Mild traumatic brain injury/concussion is a common sports injury, particularly in football players. This study found that the expression of certain serum microRNAs can distinguish concussed players from non-concussed players, indicating their potential as biomarkers for early diagnosis. The research also identified miRNAs associated with the acute phase of concussion and those with persistent changes up to 4 months post-injury.
FRONTIERS IN NEUROLOGY
(2023)
Article
Veterinary Sciences
Matias Aguilera-Rojas, Soroush Sharbati, Torsten Stein, Mario Candela Andrade, Barbara Kohn, Ralf Einspanier
Summary: This study investigated the effects of haemolysis on circulating miRNAs in dogs, and found that some miRNAs were significantly sensitive to haemolysis. The study also revealed differences in canine miRNA abundance compared to human miRNA. These findings have important implications for the discovery of circulating biomarkers in dogs, and emphasize the need to carefully consider the impact of haemolysis in serum or plasma-based tests.
BMC VETERINARY RESEARCH
(2022)
Article
Immunology
Elizabeth A. Holzhausen, Allison Kupsco, Bridget N. Chalifour, William B. Patterson, Kelsey A. Schmidt, Pari Mokhtari, Andrea A. Baccarelli, Michael I. Goran, Tanya L. Alderete
Summary: Breast milk contains bioactive compounds such as EV-miRNAs, which may regulate infant immune system development and metabolism. This study examined the associations between EV-miRNA expression and various factors, and identified factors such as sequencing quality indicators, breastfeeding practices, and maternal characteristics that are associated with EV-miRNA expression. These findings provide important insights for future breast milk miRNA research.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Lusheng Fan, Bin Gao, Ye Xu, Nora Flynn, Brandon Le, Chenjiang You, Shaofang Li, Natalia Achkar, Pablo A. Manavella, Zhenbiao Yang, Xuemei Chen
Summary: The study reveals a dual role of AAR2 in plant growth and development by promoting miRNA biogenesis and premessenger RNA splicing. AAR2 interacts with HYL1 and regulates its activity and the accumulation of miRNA.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Emilio Nunez-Borque, Sergio Fernandez-Bravo, Pablo Rodriguez Del Rio, Lucia Palacio-Garcia, Angela Di Giannatale, Virginia Di Paolo, Angela Galardi, Marta Colletti, Luisa Pascucci, Jaime Tome-Amat, Javier Cuesta-Herranz, Maria Dolores Ibanez-Sandin, Jose Julio Laguna, Alberto Benito-Martin, Vanesa Esteban
Summary: This study investigates the involvement of miRNAs in anaphylactic reactions and their potential as biomarkers. The researchers found differential expression profiles of circulating miRNAs in patients with anaphylaxis, particularly miR-375-3p. Functional and molecular experiments demonstrated that miR-375-3p plays a negative role in the stability of the endothelial barrier. These findings provide insights into the molecular mechanisms underlying anaphylactic reactions and suggest the potential of miR-375-3p as a biomarker.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, General & Internal
Valentina De Nunzio, Rossella Donghia, Pasqua L. Pesole, Sergio Coletta, Nicola Calo, Maria Notarnicola
Summary: The location of the tumor in colorectal cancer is crucial in determining disease progression, with right-sided and left-sided colon tumors showing distinct clinical and molecular characteristics. Dysregulation of proinflammatory cytokines (TGF-β, TNF-α) and PPAR-γ, as well as changes in miRNA expression, are key pathways involved in the development of this cancer. Analysis of circulating levels of TGF-β, TNF-α, PPAR-γ, and specific miRNAs in the serum can help identify specific characteristics of colorectal cancer patients, aiding in personalized treatment selection.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemical Research Methods
Yongxin Zhao, Zheng Kuang, Ying Wang, Lei Li, Xiaozeng Yang
Summary: The past two decades have witnessed a surge in studies on microRNAs (miRNAs) in plants and animals. This review article focuses on the progress and challenges of miRNA annotation in plants, comparing it with the annotation of animal miRNAs. Methods and criteria for plant miRNA annotation are discussed along with the potential solutions to existing difficulties, as well as the advantages and disadvantages of bioinformatics tools used in this field. Additionally, the availability of databases hosting plant miRNAs and potential optimization solutions are summarized, with a look towards future challenges and perspectives in plant miRNA annotations.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Cell Biology
Aneta Szudy-Szczyrek, Radoslaw Mlak, Michal Mielnik, Marcin Mazurek, Sylwia Chocholska, Martyna Podgajna, Michal Szczyrek, Iwona Homa-Mlak, Teresa Malecka-Massalska, Marek Hus
Summary: High expression of miRNA-8074 is significantly associated with poor prognosis in multiple myeloma patients.
Review
Biology
Anais N. Bauer, Niska Majumdar, Frank Williams, Smit Rajput, Lok R. Pokhrel, Paul P. Cook, Shaw M. Akula
Summary: Viral outbreaks pose obstacles to human health, and understanding viral pathology is crucial for developing methods to combat infection. The relatively new field of miRNA research in virology has shown that miRNAs play important roles in gene expression regulation. Changes in miRNA expression can either help the host fight infections or be exploited by viruses to enhance infection. Uncovering these interactions and their implications can guide therapeutic and diagnostic advancements. miRNA technology has market potential and is already being used in commercial biopharmaceuticals. Further research into the functions of miRNAs during infection and their therapeutic potential can contribute to strategies against current and future viral pathogens.
Article
Biochemistry & Molecular Biology
Tomasz Wysocki, Agnieszka Paradowska-Gorycka
Summary: Rheumatoid arthritis is a common inflammatory arthritis in which tumor necrosis factor plays a key role. However, there is variation in response to TNF-targeting drugs, which may be influenced by genetic factors. The field of pharmacogenomics offers potential for developing novel prognostic tools.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Tomasz Kmiolek, Agnieszka Paradowska-Gorycka
Summary: In recent years, there has been increasing attention on the epigenetic dysregulation in autoimmune rheumatic diseases. MicroRNAs (miRNA) have been identified as key regulators of immune cell development and function, and could potentially serve as biomarkers for RA diagnosis, prognosis, and treatment.
Review
Oncology
Daryush Purrahman, Arshid Yousefi Avarvand, Agnieszka Paradowska-Gorycka, Najmaldin Saki, Hossein Karimpourian, Hossein Jodat, Mohammad-Reza Mahmoudian-Sani
Summary: Breast cancer is a common cancer among women, and its exact cause is still unknown. The association between human papillomavirus (HPV) and breast cancer has been explored in numerous studies, but contradictory results have been obtained. This review examines the existing evidence and hypotheses about the association between HPV infection and breast cancer, as well as the potential mechanisms involved.
Article
Biochemistry & Molecular Biology
Agnieszka Paradowska-Gorycka, Anna Wajda, Ewa Rzeszotarska, Tomasz Kmiolek, Barbara Stypinska, Ewa Dudek, Katarzyna Romanowska-Prochnicka, Piotr Syrowka
Summary: Circulating free-cell miRNAs have become increasingly important as potential non-invasive biomarkers, particularly in the field of oncology. This research aimed to investigate the association between selected miRNAs in serum and the expression of Th17/Treg transcription factors, as well as clinical features, in rheumatoid arthritis (RA) patients. The study found that miR-10 was only detected in RA patients and its expression was correlated with IL-35 serum concentration and STAT5a mRNA level. Additionally, miR-326 was significantly upregulated in RA patients with rheumatoid factor presence. The differential association of transcription factor expression with serum miRNA levels may have implications for the diagnosis and progression of RA and osteoarthritis (OA).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Anna Wajda, Marcela Walczyk, Ewa Dudek, Barbara Stypinska, Aleksandra Lewandowska, Katarzyna Romanowska-Prochnicka, Marek Chojnowski, Marzena Olesinska, Agnieszka Paradowska-Gorycka
Summary: Systemic sclerosis (SSc) is a rare multisystem chronic disease with autoimmune features. In this study, the expression profile of selected microRNAs, including miR-126, -132, -143, -145, -155, -181a, -29a and -3148, was investigated in SSc patients compared to healthy controls. The results showed that the expression of these microRNAs was significantly altered in SSc patients, with miR-181a downregulated and miR-132, miR-143, miR-145 and miR-155 overexpressed. These findings suggest that these microRNAs may serve as potential biomarkers for SSc diagnosis and disease progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Biochemistry & Molecular Biology
Przemyslaw Kotyla, Marzena Olesinska
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Pharmacology & Pharmacy
Anna Wajda, Carlo Perricone, Anniruddh Kashyap, Mahmud Yerima Iliyasu
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Ewa Kuca-Warnawin, Weronika Kurowska, Magdalena Plebanczyk, Anna Wajda, Anna Kornatka, Tomasz Burakowski, Iwona Janicka, Piotr Syrowka, Urszula Skalska
Summary: Rheumatoid arthritis (RA) and osteoarthritis (OA) are joint diseases associated with expansion of pathogenic T lymphocytes. Mesenchymal stem cells provide a potential therapeutic option due to their regenerative and immunomodulatory abilities. This study evaluates the phenotype, regenerative potential and effects of adipose-derived stem cells (ASCs) from the infrapatellar fat pad (IFP) of RA and OA patients on CD4+ T cell proliferation.
Article
Biochemistry & Molecular Biology
Gabriela Filipowicz, Anna Wajda, Barbara Stypinska, Tomasz Kmiolek, Anna Felis-Giemza, Sandra Stanczyk, Zenobia Czuszynska, Marcela Walczyk, Marzena Olesinska, Agnieszka Paradowska-Gorycka
Summary: Mixed connective tissue disease (MCTD) is a rare disorder with unclear pathogenesis. This study analyzed whole blood samples from MCTD, SSc, SLE patients, and healthy donors, and found that hypomethylation distinguishes MCTD patients from SSc and SLE patients. However, global DNA methylation may not be a good diagnostic marker to distinguish MCTD from other ACTDs patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Anna Wajda, Diana Bogucka, Barbara Stypinska, Marcin Jerzy Radkowski, Tomasz Targowski, Ewa Dudek, Tomasz Kmiolek, Ewa Modzelewska, Agnieszka Paradowska-Gorycka
Summary: The present study analyzed the association between selected prostaglandin pathway genes and clinical parameters in patients with sarcopenia and frailty syndrome. The results showed a significant effect of age on PTGER4 gene expression and a negative correlation between PTGER4 expression and appendicular skeletal muscle mass. PTGES2 and PTGS2 expressions were negatively correlated with creatine phosphokinase and positively correlated with the functional mobility test timed up and go scale. In the older control group, a negative association between iron levels and the expression of PTGS2 was observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Adam Ejma-Multanski, Anna Wajda, Agnieszka Paradowska-Gorycka
Summary: Cell cultures play a significant role in the research and treatment of autoimmune connective tissue diseases (ACTDs). Culturing different cell types involved in ACTDs helps broaden knowledge of these diseases, potentially leading to finding cures. By analyzing gene expression patterns, surface proteins, and cytokine profiles in cell cultures, potential biomarkers can be identified for diagnosing, monitoring disease activity, and predicting treatment response. Moreover, cell culturing allows evaluation of the effects of therapies on patient-specific cell populations, aiding in personalized medicine.
Article
Rheumatology
Magdalena Chmielinska, Marzena Olesinska, Anna Felis-Giemza, Agnieszka Paradowska-Gorycka, Karolina Palej, Julita Rejmer-Szczesniak, Dariusz Szukiewicz
Summary: Based on the ASAS-EULAR recommendations, patients with axial spondyloarthritis who have failed to respond to at least two courses of NSAID therapy may be considered for modified NSAID treatment. This study aimed to identify risk factors for treatment failure and found that higher zonulin levels, ankylosing spondylitis, X-ray sacroiliitis, magnetic resonance imaging sacroiliitis, long duration of symptoms, high BASDAI score, and high spinal pain intensity were associated with treatment failure. Additionally, positive correlations were observed between haptoglobin concentration with C-reactive protein and erythrocyte sedimentation rate, and between zonulin levels and white blood count.
RHEUMATOLOGY INTERNATIONAL
(2023)
Article
Rheumatology
Anna Felis-Giemza, Agata Matusiewicz, Anna Wajda, Marzena Olesinska
Summary: This study evaluated the safety of esthetic medicine procedures in patients with AIRD and found that most patients underwent these procedures during remission or low disease activity. Adverse events were mostly mild and transient site reactions, and most patients expressed satisfaction with the results.
RHEUMATOLOGY INTERNATIONAL
(2023)