Journal
ROYAL SOCIETY OPEN SCIENCE
Volume 6, Issue 12, Pages -Publisher
ROYAL SOC
DOI: 10.1098/rsos.191268
Keywords
protein tags; monovalent; covalent; DNA-PAINT imaging; superresolution
Categories
Funding
- ARC Centre of Excellence in Advanced Molecular Imaging [CE140100011]
- National Health and Medical Research Council of Australia [1059278, 1037320, 1091261]
- Australian Research Council [LP140100967, DP130100269, FL150100060]
- ARC Centre of Excellence in Convergent Bio-Nano Science and Technology [CE140100036]
- Australian Government Research Training Program (RTP) Scholarship
- Australian Research Council [LP140100967] Funding Source: Australian Research Council
Ask authors/readers for more resources
Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available