Article
Chemistry, Multidisciplinary
Xin Yan, Si Chen, Zhengyan Pan, Weichen Zhao, Yukui Rui, Lijuan Zhao
Summary: Seeds are facing more difficult conditions due to climate change. Enhancing seeds' stress resilience is crucial for reducing yield loss. In this study, researchers propose using ROS-generating nanoparticles (NPs) to stimulate seeds, which can enhance their stress resilience by triggering stress/immune responses. The results show that pre-stimulated seeds with AgNPs exhibit faster germination, increased seedling vigor, biomass, and root length compared to seeds treated with hydropriming. Additionally, the researchers found that AgNPs stimulation activated stress signaling and defense-related pathways in rice seeds, and this stress memory can last weeks, providing protection to rice seedlings against salt stress and rice blast fungus. This simple nanobiostimulant-based strategy can contribute to sustainable agriculture by maintaining agricultural production and reducing the use of pesticides.
Review
Immunology
Tyler J. Ripperger, Deepta Bhattacharya
Summary: The immune protection against infections and vaccines largely relies on the neutralizing-antibody-mediated immunity conferred by memory B cells and plasma cells. Understanding the unique roles of these cells and the factors controlling their formation and persistence are crucial during the current pandemic of coronavirus disease 2019. Research on the transcriptional and metabolic programs supporting B cell fates and functions can provide insights into the intersection of these pathways.
ANNUAL REVIEW OF IMMUNOLOGY, VOL 39
(2021)
Article
Cell Biology
Yuan Li, Zhi-Qiang Luo, Jie Yuan, Sheng Wang, Juan Liu, Ping Su, Jun-Hui Zhou, Xiang Li, Jian Yang, Lan-Ping Guo
Summary: Plant stress memory can enhance plant protection and adaptation mechanisms, as demonstrated in this study on Sorbus pohuashanensis suspension cells exposed to recurrent and non-recurrent yeast extract stresses. Improved cell wall integrity and antioxidation capacity, along with significant changes in metabolites and gene expression related to phenylpropanoid biosynthesis, suggest the critical role of memory responses in plant adaptation to biotic stress.
Review
Biochemistry & Molecular Biology
Gioacchino Natoli, Francesco Pileri, Francesco Gualdrini, Serena Ghisletti
Summary: Macrophages respond to microbial and endogenous danger signals by activating various effector and homeostatic responses. This involves rapid changes in gene expression programs and metabolic rewiring, with chromatin modifications integrating transcriptional and metabolic regulation. The mutual influences between signal-induced metabolic changes and gene expression are not fully understood yet, and further research is needed to explore these interactions.
Article
Biochemistry & Molecular Biology
Ilinca Suciu, Johannes Delp, Simon Gutbier, Anna-Katharina Ueckert, Anna-Sophie Spreng, Philipp Eberhard, Christiaan Karreman, Falk Schreiber, Katrin Madjar, Joerg Rahnenfuehrer, Ivana Celardo, Ivano Amelio, Marcel Leist
Summary: Proteasome inhibition is related to parkinsonian pathology and dopaminergic neuron degeneration. In this study, the metabolome and transcriptome regulations of human LUHMES neurons exposed to MG-132 were investigated, revealing compromised energy metabolism and activation of stress response pathways. These findings provide insights into the cellular responses to proteasome dysfunction and potential therapeutic interventions for Parkinson's disease.
Article
Biochemistry & Molecular Biology
Franziska J. Heinkele, Bowen Lou, Vanessa Erben, Katrin Bennewitz, Gernot Poschet, Carsten Sticht, Jens Kroll
Summary: Obesity is a global public health issue that affects 80% of type 2 diabetes cases. Zebrafish is a common model organism for studying obesity and diabetes, with swim training being a valuable tool to study physical activity effects. Training increased zebrafish maximum speed and had metabolic and transcriptional impacts, but was not effective in preventing weight gain in overfed fish.
Article
Endocrinology & Metabolism
David A. Salisbury, David Casero, Zhengyi Zhang, Dan Wang, Jason Kim, Xiaohui Wu, Laurent Vergnes, Aashiq H. Mirza, Paola Leon-Mimila, Kevin J. Williams, Adriana Huertas-Vazquez, Samie R. Jaffrey, Karen Reue, Jianjun Chen, Tamer Sallam
Summary: Salisbury et al. demonstrate that m(6)A modifications play a sex-dependent and dietary-dependent role in regulating hepatic lipid metabolism, triaging lipogenic transcripts for degradation and preventing hepatic triglyceride accumulation. The loss of m(6)A control in male livers under lipid-rich conditions leads to a more 'feminized' hepatic lipid composition, which can be significantly diminished by liver-specific deletion of the m(6)A complex protein Mettl14 in both male and female mice. Additionally, the m(6)A installing machinery is subject to transcriptional control by the sex-responsive BCL6-STAT5 axis in response to dietary conditions, providing insights into the molecular basis for sex-specific differences in hepatic lipid traits.
Article
Plant Sciences
Tingting Sun, Junke Zhang, Qiang Zhang, Xingliang Li, Minji Li, Yuzhang Yang, Jia Zhou, Qinping Wei, Beibei Zhou
Summary: This study compared physiological, transcriptomic, and metabolic analyses in apple seedlings under different potassium (K) conditions. The results reveal that K deficiency and excess conditions affect various aspects of apple development and metabolism. Transcriptome and metabolite analyses suggest that apple plants regulate gene expression and carbon metabolism to cope with low and high K stresses. This research provides insights into the metabolic processes involved in K response and offers a foundation for improving K utilization efficiency in apples.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Na Ding, Raul Huertas, Ivone Torres-Jerez, Wei Liu, Bonnie Watson, Wolf-Rudiger Scheible, Michael Udvardi
Summary: This study found that switchgrass responds to phosphorus limitation by affecting overall plant growth, root/shoot ratio, root branching and gene transcription, with stress-induced genes involved in transcriptional regulation, metabolism, and transport processes associated with phosphorus acquisition and homeostasis. Metabolite profiling also revealed changes in levels of sugars, organic acids and amino acids under different levels of phosphorus stress.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Environmental Sciences
Feng Yu, Yawei Shen, Wenzhu Peng, Nan Chen, Yang Gan, Qizhen Xiao, Junyu Liu, Yisha Lu, Weihong Lin, Zhaofang Han, Xuan Luo, Weiwei You, Caihuan Ke
Summary: Global warming poses a threat to aquatic systems and organisms. This study compares the physiological, metabolic, and transcriptional responses of northern and southern populations of Pacific abalone to long-term temperature acclimation to understand their vulnerability and acclimation potential. The tolerant southern population exhibited a greater capacity for metabolic regulation and energy redistribution, while the vulnerable northern population could enhance its thermal tolerance through natural selection. This study provides insights into the acclimation potential of abalone to heat stress and reveals the molecular and metabolic traits underlying this phenomenon.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biotechnology & Applied Microbiology
Cholpisit Kiattisewee, Chen Dong, Jason Fontana, Widianti Sugianto, Pamela Peralta-Yahya, James M. Carothers, Jesse G. Zalatan
Summary: This study successfully transferred the CRISPRa system to P. putida by optimizing the expression levels of CRISPRa system components and applying rules based on systematic characterization of promoter features, enabling regulation of biosynthesis in the biopterin and mevalonate pathways. Multiple genes can be activated simultaneously by targeting multiple promoters or a single promoter in a multi-gene operon, paving the way for new metabolic engineering strategies in P. putida and other bacterial species.
METABOLIC ENGINEERING
(2021)
Article
Medicine, Research & Experimental
Connar S. J. Westgate, Hannah F. Botfield, Zerin Alimajstorovic, Andreas Yiangou, Mark Walsh, Gabrielle Smith, Rishi Singhal, James L. Mitchell, Olivia Grech, Keira A. Markey, Daniel Hebenstreit, Daniel A. Tennant, Jeremy W. Tomlinson, Susan P. Mollan, Christian Ludwig, Ildem Akerman, Gareth G. Lavery, Alexandra J. Sinclair
Summary: This study found that idiopathic intracranial hypertension (IIH) is a metabolic disorder characterized by dysfunction of adipose tissue. Patients with IIH exhibit insulin and leptin resistance, along with other metabolic abnormalities such as preferential central adiposity.
Article
Biology
Pau Pascual-Garcia, Shawn C. Little, Maya Capelson
Summary: Cells are able to develop transcriptional memory after repeated exposure to external cues, and this memory can be maintained epigenetically through cell divisions with the help of a nuclear pore component called Nup98. This study used single-molecule RNA FISH to examine the dynamics of transcription in Drosophila cells upon repeated exposure to the steroid hormone ecdysone. The results showed that cells rapidly activate a low-level transcriptional response upon hormone exposure, but also slowly transition into a specialized memory state characterized by a high rate of expression. It was found that this transition between non-memory and memory states is independent of the initial activation of transcription.
Article
Biochemistry & Molecular Biology
Bu-Yeo Kim, Seo-Young Lee, Sun-Ku Chung
Summary: This study revealed that the polymorphic p53 codon 72 does not significantly affect growth arrest or apoptosis, but differentially regulates the expression patterns related to metabolism in cells exposed to genotoxic stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Multidisciplinary Sciences
Jason H. Brickner
Summary: This article discusses the mechanisms by which histone modifications can be inherited and relates them to the phenomenon of epigenetic transcriptional memory. Recent studies have found that the histone H3 lysine 4 dimethylation associated with this memory plays a critical role in sustaining memory and can be stably maintained through multiple mitoses. This chromatin-mediated inheritance mechanism involves a physical interaction between an H3K4me2 reader, SET3C, and an H3K4me2 writer, Spp1(-) COMPASS. This is the first example of chromatin-mediated inheritance of a mark that promotes transcription.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2023)
Article
Endocrinology & Metabolism
Liujun Chen, Lisha Li, Donghong Cui, Yiheng Huang, Haibin Tong, Haleh Zabihi, Shuxia Wang, Yadan Qi, Ted Lakowski, Lin Leng, Suixin Liu, Hong Wu, Lawrence H. Young, Richard Bucala, Dake Qi
Summary: Attenuation of adipose hormone sensitive lipase (HSL) may impair lipolysis and exacerbate obesity. Cytokine macrophage migration inhibitory factor (MIF) plays a role in regulating adipose HSL and adipocyte hypertrophy. Both intracellular and extracellular MIF have opposing effects on HSL, but extracellular action predominates to downregulate HSL and exacerbate obesity development during high-fat diet (HFD).
MOLECULAR METABOLISM
(2024)
Article
Endocrinology & Metabolism
Mengyang Tang, Yi Zhang, Rong Zhang, Yuemei Zhang, Jiangfei Zheng, Daixi Wang, Xinyu Wang, Jing Yan, Cheng Hu
Summary: This study aimed to explore the role of GPSM1 in POMC neurons and the underlying mechanisms in metabolic homeostasis. Through various molecular, biochemical, immunofluorescent, immunohistochemical analyses, and cell culture studies, the study revealed the pathophysiological role of GPSM1 in POMC neurons and its regulation of POMC neuron activity.
MOLECULAR METABOLISM
(2024)
