Journal
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
Volume 30, Issue 1, Pages 32-35Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/13102818.2015.1088401
Keywords
DNA extraction; rice; seeds
Categories
Funding
- Natural Science Foundation of Hubei Province [BZY13021]
- Fundamental Research Funds for the Central Universities of China [CZZ12006]
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Rapid and inexpensive preparation of genomic DNA from rice seeds for marker-assisted selection and seed purity estimation is a major bottleneck for plant breeders. Here, we describe a high-throughput method that provides DNA at sufficient quantity and quality for these applications. Optimization of buffer composition and individual protocol stages allow processing of 384 samples within 2 h, yielding templates that reliably support downstream polymerase chain reaction of single copy amplicons up to 1.2 kb.
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