Article
Oncology
Nicoletta Nastasi, Amada Pasha, Gennaro Bruno, Angela Subbiani, Laura Pietrovito, Angela Leo, Lucia Scala, Lorena de Simone, Gabriella Casazza, Federica Lunardi, Maria Letizia Taddei, Angela Tamburini, Annalisa Tondo, Claudio Favre, Maura Calvani
Summary: Osteosarcoma is a common malignant bone tumor that mainly affects children and adolescents. Standard treatment includes surgery and chemotherapy, but the effectiveness is limited for metastatic or recurrent cases. This study investigates the efficacy of mifamurtide and identifies interleukin-10 as a possible target to improve its effectiveness. The synergistic use of mifamurtide and an anti-IL-10 antibody significantly increases cell death and reduces metastasis in a mouse model. The findings suggest that combining mifamurtide with an anti-IL-10 antibody may improve outcomes for osteosarcoma patients.
Review
Biochemistry & Molecular Biology
Roxana-Olimpia Gheorghe, Andreea Violeta Grosu, Melania Bica-Popi, Violeta Ristoiu
Summary: Traumatic peripheral neuropathic pain is a complex syndrome caused by primary peripheral nervous system dysfunction or damage. The interaction between neurons and macrophages plays a crucial role in pain development and chronicization, but it can also promote injury resolution and pain reduction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Junad Khan, Qian Wang, Yanfang Ren, Rotem Eliav, Olga A. Korczeniewska, Rafael Benoliel, Eli Eliav
Summary: The exercise-induced hypoalgesia (EIH) profile was found to predict pain behavior following nerve injury, with low EIH rats developing more severe allodynia and hyperalgesia. IL-1beta may be associated with painful neuropathy developed in rats with low EIH, while the anti-inflammatory cytokine IL-10 may have a protective role against pain development.
Article
Biochemistry & Molecular Biology
Marco Orecchioni, Dennis Wolf, Vasantika Suryawanshi, Holger Winkels, Kouji Kobiyama, Jeffrey Makings, William B. Kiosses, Klaus Ley
Summary: Atherosclerosis is caused by the retention of lipoproteins and cholesterol in the subendothelial layer, leading to an inflammatory process and plaque formation. Macrophages are found to be the main producers of the anti-inflammatory cytokine IL-10 in atherosclerotic plaques. IL-10 secretion by macrophages is critical for limiting atherosclerosis progression.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Immunology
Erliang Kong, Yongchang Li, Mengqiu Deng, Tong Hua, Mei Yang, Jian Li, Xudong Feng, Hongbin Yuan
Summary: Neuropathic pain is characterized by hyperalgesia and allodynia. Inflammation plays a role in the development of neuropathic pain, affecting synaptic transmission and regulated by glial cells. Glycometabolism reprogramming in inflammatory cells, specifically impaired oxidative phosphorylation and enhanced glycolysis, promotes neural inflammation in peripheral and central nervous system. Further research is needed to explore the role of glycometabolism in glial cells in neuropathic pain.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Laura Lopez-Gomez, Agata Szymaszkiewicz, Marta Zielinska, Raquel Abalo
Summary: Glia cells, previously seen as merely structural support for neurons, have been found to be equally as important, with enteric glial cells in the gastrointestinal tract playing essential roles in neuroprotection and immune system regulation. Nutraceuticals, defined as food substances providing health benefits, have shown potential as alternative methods for preventing and treating enteric gliopathies and associated gastrointestinal conditions, although studies in this area are currently limited.
Article
Immunology
Qingyu Sun, Tingting Hu, Yurui Zhang, Xiaotong Wang, Jing Liu, Wen Chen, Chao Wei, Dianxin Liu, Weihua Wu, Ting Lan, Yumeng Ding, Zhaoli Luo, Meng Liu, Danmin Shen, Zhongnan Xiao, Liye Hu, Miaoyi Pang, Yiran Ma, Lei Shi, Peipei Wang, Jiannan Zhang, Qian Li, Fei Yang
Summary: In this study, the researchers found that itaconate has an analgesic effect in neuropathic pain models, mediated through the activation of the spinal IL-10/STAT3/beta-endorphin pathway. Exogenous administration of itaconate can alleviate neuropathic pain, while endogenous itaconate is associated with inflammation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Anett Mazlo, Ramona Kovacs, Noemi Miltner, Marta Toth, Zoltan Vereb, Krisztina Szabo, Ildiko Bacskai, Kitti Pazmandi, Agota Apati, Tamas Biro, Krisztian Bene, Eva Rajnavolgyi, Attila Bacsi
Summary: Findings suggest that MSCl cells promote differentiation of CTLA-4 expressing DCs through the production of all-trans retinoic acid, leading to the activation and polarization of allogeneic, naive T cells by semi-matured DCs in a CTLA-4 dependent manner. Understanding the molecular mechanisms behind MSC-mediated modulation of DC differentiation may expand the clinical application of MSCs in cell-free therapies.
Article
Immunology
Sara Francisco, Alicia Arranz, Javier Merino, Carmen Punzon, Rosario Perona, Manuel Fresno
Summary: The study found that TLR2 ligands activate MAPKs p38 and ERK earlier in macrophages, leading to higher levels of IL-10/IL-12 and IL-10/TNF-alpha ratios compared to TLR4 ligand LPS. Early TLR2-mediated p38 induction contributes to high IL-10 production, likely suppressing host Th1 response against certain pathogens.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Meng-Yan Deng, Khalil Ali Ahmad, Qiao-Qiao Han, Zi-Ying Wang, Rana Muhammad Shoaib, Xin-Yan Li, Yong-Xiang Wang
Summary: The study showed that thalidomide alleviates neuropathic pain by upregulating spinal microglial IL-10 and beta-endorphin expression rather than downregulating TNF alpha expression.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Immunology
Khalil Ali Ahmad, Rana Muhammad Shoaib, Muhammad Zaeem Ahsan, Meng-Yan Deng, Le Ma, Evhy Apryani, Xin-Yan Li, Yong-Xiang Wang
Summary: Gabapentinoids alleviate neuropathic pain by stimulating the expression of spinal microglial IL-10 and consequent beta-endorphin.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Immunology
Raquel Tonello, Arthur Silveira Prudente, Sang Hoon Lee, Cinder Faith Cohen, Wenrui Xie, Aditi Paranjpe, Jueun Roh, Chul-Kyu Park, Gehoon Chung, Judith A. Strong, Jun-Ming Zhang, Temugin Berta
Summary: Using single-cell RNA sequencing, this study identified a transcriptional profile for satellite glial cells (SGCs) and found enriched expression of TIMP3 and other metalloproteinases in SGCs. Experiments showed that TIMP3 modulates somatosensory stimuli and that paclitaxel directly impacts metalloproteinase signaling in cultured SGCs, suggesting potential new treatments for pain.
BRAIN BEHAVIOR AND IMMUNITY
(2023)
Article
Medicine, Research & Experimental
Gisele Graca Leite dos Santos, Anna Lethicia Lima Oliveira, Dourivaldo Silva Santos, Renan Fernandes do Espirito Santo, Daniela Nascimento Silva, Paulo Jose Lima Juiz, Milena Botelho Pereira Soares, Cristiane Flora Villarreal
Summary: The study found that MSC can completely reverse the mechanical allodynia and thermal hyperalgesia induced by OXL-induced sensory neuropathy. MSC increased the gene expression of antioxidant factors SOD and Nrf-2, elevated the levels of anti-inflammatory cytokines IL-10 and TGF-beta in the spinal cord of neuropathic mice, and decreased nitrite and MDA levels, possibly inducing the reversal of OXL-induced sensory neuropathy by activating anti-inflammatory and antioxidant pathways.
Article
Biochemistry & Molecular Biology
Ashok Patidar, Sathishkumar Selvaraj, Mohona Chakravarti, Ipsita Guha, Avishek Bhuniya, Saurav Bera, Sukanya Dhar, Kamalika Roy, Rathindranath Baral, Debprasad Chattopadhyay, Chiranjib Pal, Bhaskar Saha
Summary: Activation of the tumor-eliminating immune response is a major challenge. Macrophages, a major component of solid tumors, play important roles in tumor development and regression. The interaction between Toll-Like Receptors (TLRs) and IL-27 has been found to enhance macrophage function and contribute to tumor regression by activating T cells. This study investigates the crosstalk between TLRs and IL-27 in a melanoma model and demonstrates the preventative role of TLR-induced IL-27 in aggressive and highly invasive melanoma.
Article
Biochemistry & Molecular Biology
Shunyi Zhao, Jiaying Zheng, Lingxiao Wang, Anthony D. Umpierre, Sebastian Parusel, Manling Xie, Aastha Dheer, Katayoun Ayasoufi, Aaron J. Johnson, Jason R. Richardson, Long-Jun Wu
Summary: Chemogenetic approaches using DREADD were employed in microglia, specifically targeting CX3CR1(+) cells. Activation of hM4Di on CX3CR1(+) cells induced hypolocomotion, which was unexpectedly preserved even when microglia were depleted. The expression of hM4Di in peripheral immune cells may be responsible for the observed hypolocomotion. Rigorous data analysis and interpretation are necessary when manipulating microglia using the Cx3cr1(CreER/+) mouse line.
MOLECULAR PSYCHIATRY
(2023)
Article
Immunology
Thaiane Pinto Moreira, Carla Daiane Ferreira de Sousa, Victor Rodrigues de Melo Costa, Celso Martins Queiroz-Junior, Franciele Martins Santos, Caio Santos Bonilha, Lisia Maria Esper, Mauricio Lacerda Nogueira, Thiago Mattar Cunha, Mauro Martins Teixeira, Vivian Vasconcelos Costa, Daniele da Gloria de Souza
Summary: This study describes a novel mouse model of CHIKV infection and evaluates the role of tumour necrosis factor in the disease's pathogenesis. It shows that TNF plays a dual role in viral clearance and driving tissue damage and hypernociception. Therefore, TNF inhibitors may have therapeutic benefits, but viral levels must be monitored and used in combination with antiviral drugs when treating CHIKV-infected patients.
Review
Physiology
Fatemeh Bagheripour, Sajad Jeddi, Khosrow Kashfi, Asghar Ghasemi
Summary: The prevalence of type 2 diabetes (T2D) is increasing worldwide. L-citrulline (Cit), a precursor of nitric oxide (NO) production, has been suggested as a potential therapeutic agent for T2D. Cit supplementation in T2D has shown positive effects on glucose, lipid, and antioxidant metabolism through multiple mechanisms, including increased insulin secretion, glucose uptake, lipolysis, and decreased glyceroneogenesis.
Article
Cell Biology
Vanessa de Fatima Borges, Leticia Selinger Galant, Alexandre Kanashiro, Fernanda Vargas e Silva Castanheira, Valter Vinicius Silva Monteiro, Diego angelo Duarte, Filipe Camargo Rodrigues, Camila Meirelles de Souza Silva, Ayda Henriques Schneider, Guilherme Cesar Martelossi Cebinelli, Mikhael Haruo Fernandes de Lima, Joao Paulo de Biaso Viola, Thiago Mattar Cunha, Claudio Miguel da Costa Neto, Jose Carlos Farias Alves-Filho, Andre Sampaio Pupo, Fernando de Queiroz Cunha
Summary: This study investigated the effects of FK506 on sepsis immunopathology and found that FK506 treatment resulted in failed neutrophil migration, leading to increased bacteremia and systemic inflammatory response, causing higher organ damage and mortality rates.
INFLAMMATION RESEARCH
(2023)
Article
Cell Biology
Marcelo Franchin, Thaise Mayumi Taira, Douglas da Silva Prado, Cesar Augusto Speck Hernandez, Fabio Bonifacio de Andrade, Henrique Ballassini Abdalla, Marcelo Henrique Napimoga, Thiago Mattar Cunha, Sandra Yasuyo Fukada, Pedro Luiz Rosalen
Summary: In this study, we investigated the modulatory effects of PI3K gamma on IL-17A expression and the progression of experimental periodontitis in vivo. Our results showed that PI3K gamma inhibitor can attenuate alveolar bone loss and downregulate IL-17A and Rankl gene expression.
INFLAMMATION RESEARCH
(2023)
Article
Infectious Diseases
Felipe Cerni, Isadora Oliveira, Francielle Cordeiro, Karla Bordon, Isabela Ferreira, Wuelton Monteiro, Eliane Arantes, Thiago Cunha, Manuela Pucca
Summary: This study evaluated the pain behavior induced by T. serrulatus venom and its isolated neurotoxins in mice, and found that different neurotoxins caused varying degrees of pain behavior. Additionally, the researchers developed a mice-derived polyclonal antibody targeting Ts5, which was able to neutralize the effect of this neurotoxin and significantly reduce the nociception produced by the whole venom. This study is the first to explore the effects of different classes of T. serrulatus neurotoxins on nociception, and demonstrates the important role of Ts5 in scorpion sting-induced pain.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Pharmacology & Pharmacy
Sergio M. Borghi, Tiago H. Zaninelli, Telma Saraiva-Santos, Mariana M. Bertozzi, Renato D. R. Cardoso, Thacyana T. Carvalho, Camila R. Ferraz, Doumit Camilios-Neto, Fernando Q. Cunha, Thiago M. Cunha, Felipe A. Pinho-Ribeiro, Rubia Casagrande, Waldiceu A. Verri
Summary: In this study, the researchers investigated the effect of pentoxifylline (Ptx) on delayed-onset muscle soreness (DOMS) in untrained mice exposed to intense acute swimming exercise. They found that Ptx treatment reduced mechanical hyperalgesia caused by intense acute swimming in a dose-dependent manner. Ptx also inhibited neutrophil recruitment, oxidative stress, and cytokine production in the muscle tissue and spinal cord, as well as reduced glial cell activation in the spinal cord. These findings suggest that Ptx can alleviate pain in DOMS by targeting peripheral and spinal cord mechanisms.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Endocrinology & Metabolism
Asghar Ghasemi, Parvin Mirmiran, Khosrow Kashfi, Zahra Bahadoran
Summary: Scientific publishing has been crucial for advancing science, but it faces challenges and negative phenomena. Developing a global collaborative non-commercial model is proposed for the future of scientific publishing.
INTERNATIONAL JOURNAL OF ENDOCRINOLOGY AND METABOLISM
(2023)
Review
Pharmacology & Pharmacy
Zahra Alimardan, Maryam Abbasi, Farshid Hasanzadeh, Mahmud Aghaei, Ghadamali Khodarahmi, Khosrow Kashfi
Summary: Heat shock proteins (Hsps), including Hsp70, Hsp90, and small Hsps, and the transcription factor FoxM1, have important roles in carcinogenesis. Hsp70 functions in cancer initiation and protein folding, and is overexpressed in human cancers. It also interacts with cochaperones and regulates the FoxM1 signaling pathway. FoxM1 is overexpressed in most human cancers and is involved in various cancer-related processes. This review covers the structure, function, and inhibitors of Hsp70, Hsp90, and FoxM1.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Cell Biology
N. B. Amaral, T. S. Rodrigues, M. C. Giannini, M. I. Lopes, L. P. Bonjorno, P. I. S. O. Menezes, S. M. Dib, S. L. G. Gigante, M. N. Benatti, U. C. Rezek, L. L. Emrich-Filho, B. A. Sousa, S. C. L. Almeida, R. Luppino-Assad, F. P. Veras, A. H. Schneider, L. O. S. Leiria, L. D. Cunha, J. C. Alves-Filho, T. M. Cunha, E. Arruda, C. H. Miranda, A. Pazin-Filho, M. Auxiliadora-Martins, M. C. Borges, B. A. L. Fonseca, V. R. Bollela, C. M. Del-Ben, F. Q. Cunha, R. C. Santana, F. C. Vilar, D. S. Zamboni, P. Louzada-Junior, R. D. R. Oliveira
Summary: This study evaluated whether colchicine treatment was associated with the inhibition of NLRP3 inflammasome activation in patients with COVID-19. The results showed that colchicine reduced the need for supplemental oxygen and the length of hospitalization, and lowered the serum levels of Casp1p20 and IL-18 in the first two days of treatment.
INFLAMMATION RESEARCH
(2023)
Article
Immunology
Tamara S. Rodrigues, Camila C. S. Caetano, Keyla S. G. de Sa, Leticia Almeida, Amanda Becerra, Augusto Goncalves, Leticia de Sousa Lopes, Samuel Oliveira, Danielle P. A. Mascarenhas, Sabrina S. Batah, Bruna M. Silva, Giovanni F. Gomes, Ricardo Castro, Ronaldo B. Martins, Jonathan Avila, Fabiani G. Frantz, Thiago M. Cunha, Eurico Arruda, Fernando Q. Cunha, Helder Nakaya, Larissa D. Cunha, Alexandre T. Fabro, Paulo Louzada-Junior, Rene D. R. de Oliveira, Dario S. Zamboni
Summary: SARS-CoV-2 infection activates the NLRP3 inflammasome and leads to inflammation and severe COVID-19. Human caspase-4 (mouse caspase-11) is upregulated and activated by SARS-CoV-2, contributing to NLRP3 activation. In vivo studies using transgenic hACE2 humanized mice show that hACE2 Casp11(-/-) mice are protected from disease development, with reduced lung damage, clinical symptoms, and mortality. Analysis of human samples from fatal COVID-19 cases reveals the expression of CASP4 in patient lungs, which correlates with inflammasome components and inflammatory mediators. These findings suggest CASP4/11 as a potential target for therapeutic interventions in COVID-19.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Biology
Rafaela M. Guimares, Conceico E. Anibal-Silva, Marcela Davoli-Ferreira, Francisco Isaac F. Gomes, Atlante Mendes, Maria C. M. Cavallini, Miriam M. Fonseca, Samara Damasceno, Larissa P. Andrade, Marco Colonna, Cyril Rivat, Fernando Q. Cunha, Jose C. Alves-Filho, Thiago M. Cunha
Summary: After peripheral nerve injury, there is an increase in the number of macrophages in the sensory ganglia due to the proliferation of resident macrophages rather than infiltration of peripheral blood monocytes. These proliferating macrophages produce pro-inflammatory cytokines and participate in the development of neuropathic pain. Signaling through CX3CR1 is involved in the proliferation of macrophages in the sensory ganglia and neuropathic pain development.
Article
Medicine, General & Internal
Miguel Trindade, Mafalda Teixeira, Joao Serodio, Catarina Favas, Jose Delgado Alves
Summary: Status epilepticus (SE) in elderly patients admitted to internal medicine wards is associated with high morbidity and mortality. This study aimed to characterize SE in this population, identify outcome predictors, and compare differences between young and elderly patients, as well as those with convulsive and non-convulsive SE. The findings showed that elderly patients had higher disability at admission, predominantly had non-convulsive SE, and different etiologies compared to younger patients. NCSE and EEG with paroxysmal activity at discharge were predictive of worse prognosis. The study concluded that SE in elderly patients should be addressed differently from standard SE approaches.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Respiratory System
Flavio P. Veras, Giovanni F. Gomes, Bruna M. S. Silva, Diego B. Caetite, Cicero J. L. R. Almeida, Camila Meirelles S. Silva, Ayda H. Schneider, Emily S. Corneo, Caio S. Bonilha, Sabrina S. Batah, Ronaldo Martins, Eurico Arruda, Alexandre T. Fabro, Jose C. Alves-Filho, Thiago M. Cunha, Fernando Q. Cunha
Summary: COVID-19 is characterized by severe lung injury due to neutrophil infiltration and release of neutrophil extracellular traps (NETs). This study demonstrates that treatment with DNase I, which degrades NETs, improves clinical symptoms and reduces lung, heart, and kidney injuries in a COVID-19 mouse model.
RESPIRATORY RESEARCH
(2023)
Article
Dermatology
Lorena Carla Oliveira da Costa, Luiz Gustavo Gardinassi, Flavio Protasio Veras, Cristiane Milanezi, Leandra Naira Zambelli Ramalho, Luciana Benevides, Jose Carlos Alves-Filho, Joao Santana da Silva, Cacilda da Silva Souza
Summary: This study found that the transcriptional factor Blimp-1 is implicated in the pathogenesis of psoriasis (PsO) by regulating the expression of genes involved in the Th17 inflammatory pathway. The expression of PRDM1 and IL17A was increased in PsO, and PRDM1 correlated positively with the expression of several cytokines. This knowledge may contribute to the development of new treatments for PsO.
ARCHIVES OF DERMATOLOGICAL RESEARCH
(2023)
