Article
Ophthalmology
Xiaowen Zhang, Shengqian Dou, Yusen Huang
Summary: This study investigated the genetic mechanisms underlying cataract formation in the diabetic population. The results showed increased RNA modification and upregulated expression of core enzymes in diabetic cataract patients. Single-cell analysis revealed a close association between early fiber cell clusters and cataract onset and RNA modification. These findings provide valuable resources for future studies on the pathogenesis of cataracts.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Zifei Liu, Lei Zhou, Dongxu Li, Honghui Lu, Liang Liu, Weijia Mao, Xiaoqing Yu, Yixuan Fan, Qunhao Huang, Feng Wang, Yongjie Wan
Summary: N6-methyladenosine (m6A) plays a crucial role in granulosa cells during oocyte maturation in Haimen goats. Knockdown of METTL3 disrupts the cell cycle in goat granulosa cells and affects cell apoptosis, proliferation, and hormone secretion. METTL3 may regulate the cell cycle by mediating AURKB mRNA degradation and participating in AURKB transcription through the CCND1-RB-E2F1 pathway.
Article
Biochemistry & Molecular Biology
Zifei Liu, Lei Zhou, Dongxu Li, Honghui Lu, Liang Liu, Weijia Mao, Xiaoqing Yu, Yixuan Fan, Qunhao Huang, Feng Wang, Yongjie Wan
Summary: This study reveals the important role of METTL3 in granulosa cells during oocyte maturation in Haimen goats. Knockdown of METTL3 disrupted the cell cycle and affected cell apoptosis, proliferation, and hormone secretion. Mechanistically, METTL3 may regulate the cell cycle by mediating AURKB mRNA degradation and participating in AURKB transcription, indicating the involvement of RNA methylation in oocyte maturation through granulosa cells.
Article
Dermatology
Tong Kang, Cheng Zhang, Hao Lei, Ruiting Luo, Meng Liu, Shengbang Wang, Xiu Zhang, Qiqi Duan, Shengxiang Xiao, Yan Zheng
Summary: This study found that NPTX2 is overexpressed in both skin lesions and cell lines of cSCC. In vitro studies showed that NPTX2 promotes cell proliferation, migration, invasion, colony formation, and epithelial.mesenchymal transition in cSCC cells. Mechanistically, NPTX2 facilitates epithelial.mesenchymal transition by promoting METTL3-mediated N6-methyladenosine modification of SNAIL. In vivo studies confirmed the oncogenic role of NPTX2 in cSCC, suggesting it may be a potential therapeutic target.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Medicine, Research & Experimental
Xiao Li, Miaomiao Sun, Anran Cheng, Guangying Zheng
Summary: DC is a major ocular complication of diabetes, characterized by EMT of LECs. GAS5 and miR-204-3p are involved in the pathogenic mechanism through regulating migration and EMT of LECs induced by HG. The dysregulation of GAS5 impacts lens epithelial cell migration and EMT under HG conditions via the miR-204-3p/TGFBR1 axis.
LABORATORY INVESTIGATION
(2022)
Article
Cell Biology
Lujia Feng, Yantao Wei, Yimeng Sun, Linbin Zhou, Shaowei Bi, Weirong Chen, Wu Xiang
Summary: Cataracts are a leading cause of blindness worldwide, with age being a major risk factor. The exact mechanisms of cataractogenesis are still unclear. A recent study found that microRNA-34a (MIR34A) is involved in cataract development, but the underlying pathogenesis is unknown. This study showed that hexokinase 1 (HK1) is a target gene of MIR34A and demonstrated that MIR34A modulates lens epithelial cell apoptosis and cataract development through the HK1/caspase 3 signaling pathway.
Article
Endocrinology & Metabolism
Lei Cai, Xiao-Yan Han, Dan Li, Dong-Mei Ma, Yu-Meng Shi, Yi Lu, Jin Yang
Summary: Despite successful surgical replacement with artificial lenses, cataracts remain a leading cause of visual disturbance and blindness worldwide. Diabetic cataract (DC), a metabolic complication, occurs at an earlier age and progresses faster than age-related cataracts. N6-methyladenosine (m6A) has been linked to DC progression, but the role of RNA m6A modifications in DC pathogenesis is not well understood.
WORLD JOURNAL OF DIABETES
(2023)
Article
Medicine, Research & Experimental
Xiao Li, Doudou Chen, Bowen Ouyang, Shengnan Wang, Yawei Li, Li Li, Siquan Zhu, Guangying Zheng
Summary: This study investigated the role of KLF5 in diabetic cataract (DC) and its underlying mechanism. The expression of KLF5 was significantly increased in DC lens, diabetic rat lens, and high glucose-stimulated human lens epithelial cells (HLECs). Knockdown of KLF5 inhibited EMT, migration, oxidative stress, and inflammation in high glucose-stimulated HLECs. KLF5 was found to bind the MDM2 promoter and enhance the transcriptional activity of MDM2. The study suggests that targeting KLF5 could be a potential therapeutic strategy for DC.
LABORATORY INVESTIGATION
(2023)
Article
Cell Biology
Jiangdong Ren, Yicheng Li, Shalitanati Wuermanbieke, Shu Hu, Guangxin Huang
Summary: The study found that an m(6)A-related lncRNA LINC00680 is upregulated in osteoarthritis tissues and chondrocytes. Silencing LINC00680 can restore chondrocyte proliferation and inhibit extracellular matrix degradation. LINC00680 affects chondrocyte function by interacting with SIRT1 mRNA and enhancing its stability.
CELL DEATH DISCOVERY
(2022)
Article
Cell Biology
Haiyuan Mu, Ting Zhang, Ying Yang, Danru Zhang, Jie Gao, Junhong Li, Liang Yue, Dengfeng Gao, Bingbo Shi, Yue Han, Liang Zhong, Xinze Chen, Zhen-Bo Wang, Zhen Lin, Ming-Han Tong, Qing-Yuan Sun, Yun-Gui Yang, Jianyong Han
Summary: m(6)A modification plays essential roles in follicle development and oocyte growth, regulating oocyte meiosis by enhancing RNA stability.
CELL DEATH & DISEASE
(2021)
Article
Medical Laboratory Technology
Xueying An, Wenshu Wu, Lin Yang, Jian Dong, Bin Liu, Junxia Guo, Jianmei Chen, Baosheng Guo, Wangsen Cao, Qing Jiang
Summary: This study found that m6A modification of mRNA plays a significant role in the epigenetic tumorigenesis of osteosarcoma. METTL3 and ZBTB7C are key regulators of m6A modification, and targeting the METTL3/ZBTB7C axis may offer potential therapeutic strategies for osteosarcoma.
TRANSLATIONAL RESEARCH
(2023)
Article
Cell Biology
Le Zhang, Xin Zhao, Jing Wang, Yanwu Jin, Moxuan Gong, Yuyang Ye, Peilong Li
Summary: In this study, the role of METTL3-regulated N6-methyladenosine in modulating primiR-150 maturation during neuropathic pain progression was demonstrated. METTL3 was found to promote m6A methylation and inhibit NP, while its downregulation exacerbated the severity of NP. The METTL3/miR-150/BDNF pathway may serve as a promising therapeutic target for NP patients.
CELL DEATH DISCOVERY
(2022)
Article
Medicine, General & Internal
Miaomiao Sun, Ke Li, Xiao Li, Huajun Wang, Li Li, Guangying Zheng
Summary: This study identified the competitive inhibition of miR-29b expression by lncRNA TUG1, leading to increased Smac release and promotion of lens epithelial cell apoptosis in age-related cataracts. This mechanism contributes to the formation of ARC and suggests a new molecular pathway for regulating ARC development.
CHINESE MEDICAL JOURNAL
(2023)
Article
Gastroenterology & Hepatology
Huarong Chen, Shanshan Gao, Weixin Liu, Chi-Chun Wong, Jianfeng Wu, Jingtong Wu, Dabin Liu, Hongyan Gou, Wei Kang, Jianning Zhai, Chuangen Li, Hao Su, Shiyan Wang, Fraser Soares, Jiahuai Han, Housheng Hansen He, Jun Yu
Summary: The study revealed that the m(6)A regulatory enzyme METTL3 is upregulated in colorectal cancer (CRC) and predicts poor survival in patients. Inhibition of METTL3 suppresses tumorigenesis in CRC cells and organoids. METTL3 promotes CRC development by activating the m(6)A-GLUT1-mTORC1 axis.
Article
Biochemistry & Molecular Biology
Yuan Zhao, Jianguo Li, Yilin Lian, Qian Zhou, Yukang Wu, Jiuhong Kang
Summary: The study found that METTL3 is not necessary for the formation and differentiation of human neural progenitor cells (hNPCs), but it inhibits hNPC proliferation by reducing SLIT2 expression. Mechanistic studies revealed that METTL3 degradation decreases the enrichment of m(6)A in SLIT2 mRNA, consequently leading to reduced expression of SLIT2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
