Article
Reproductive Biology
Kenji Vann, Adelaide E. Weidner, Ariana C. Walczyk, Olga Astapova
Summary: Granulosa cell-specific knockout of adaptor protein paxillin in mice increases fecundity and may reduce granulosa cell death. Knockout of paxillin in granulosa cells leads to increased fertility in older reproductive age mice, which is not due to increased numbers of estrous cycles, ovulated oocytes per cycle, or pups per litter, but rather shorter time to pregnancy and increased number of litters.
BIOLOGY OF REPRODUCTION
(2023)
Article
Obstetrics & Gynecology
Barbora Loncova, Zuzana Fabova, Milos Mlyncek, Alexander V. Sirotkin
Summary: This study investigated the release of epiregulin (EREG) by human ovarian granulosa cells, its direct impact on basic ovarian cell functions, and its relationship with gonadotropins. The results showed a time-dependent accumulation of EREG in the medium incubated with human ovarian granulosa cells, peaking at 3 to 4 days. The addition of EREG alone increased cell viability, proliferation, and hormone release, while decreasing apoptosis. Furthermore, the gonadotropins FSH and LH enhanced the stimulatory effect of EREG on granulosa cell functions.
REPRODUCTIVE SCIENCES
(2023)
Article
Endocrinology & Metabolism
Yan Deng, Hu Li, Yi Song, Juan Cen, Yuying Zhang, Yi Sui, Dexuan Cui, Tin Chiu Li, Yan Xu, Chi Chiu Wang, Pui Wah Jacqueline Chung, Tao Tang
Summary: This study investigated the lncRNA and mRNA profiles in ovarian granulosa cells from women with PCOS and identified DLGAP5 as a dysregulated candidate gene involved in the pathogenesis of PCOS, particularly in granulosa cell apoptosis and proliferation.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Multidisciplinary Sciences
Yi-Fan Jiang, Pin-Huan Yu, Yovita Permata Budi, Chih-Hsien Chiu, Chi-Yu Fu
Summary: This study investigated the dynamics of mitochondria in mammalian ovaries during folliculogenesis and luteal formation. The results showed that mitochondrial networks shifted from branched structures in follicles to spherical and tubular mitochondria in large luteal cells. Additionally, mitochondrial diameter and volume increased from folliculogenesis to luteal formation, indicating mitochondrial enlargement as an event during these processes. Modulation of mitochondrial morphology and respiratory enzyme expressions may be related to tissue remodeling during luteal formation.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Alexander V. Sirotkin, Martina Macejkova, Adam Tarko, Zuzana Fabova, Saleh Alwasel, Abdel Halim Harrath
Summary: The study found that xylene suppresses ovarian cell functions, but buckwheat, rooibos, and vitex can mitigate these effects, acting as natural protectors against the adverse effects of xylene on ovarian cells.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Hairui Fan, Shiqin Wang, Haifei Wang, Mingan Sun, Shenglong Wu, Wenbin Bao
Summary: These findings indicate that DON negatively impacts murine ovary GCs, while melatonin can counteract these effects by reducing oxidative stress, improving mitochondrial function, and reducing inflammation. The study sheds light on the protective effects of melatonin against mycotoxin contamination.
Article
Pharmacology & Pharmacy
Jieyu Cui, Yankun Li, Wenqiang Zhang, Hongrun Qian, Zhiying Zhang, Kun Xu
Summary: This study investigated the impact of alginic acid (AA) on the ovary and its cellular mechanism. The results showed that AA down-regulated the levels of estradiol and progesterone, induced oxidative stress, mitochondrial-mediated apoptosis, and autophagy. The animal experiments also confirmed the toxicity of AA on the ovary, resulting in a shortened estrus period in mice. These findings provide scientific basis for controlling the increasing use of AA.
Article
Biochemistry & Molecular Biology
IrantzuADD F. B. Perez-Ruiz, Jose-Ignacio Ruiz-Sanz, Maria-Luisa Hernandez, Rosaura Navarro, Marcos Ferrando, Zaloa Larreategui, Maria-Begona Ruiz-Larrea
Summary: This study provided the first evidence of the presence of mRNAs and proteins of the three members of the PON family in human ovarian granulosa cells, with active secretion of PON1 and PON3 by the cells. Additionally, it was discovered that PON2 mainly localized with mitochondria and the subcellular nucleo-cytoplasmic distribution of PON1 was associated with the cell cycle.
Article
Obstetrics & Gynecology
Xiao Xu, Hao-Ran Shen, Min Yu, Mei-Rong Du, Xue-Lian Li
Summary: The study revealed that let-7i is down-regulated in PCOS granulosa-luteal cells, and overexpression of let-7i inhibits cell proliferation and decreases estradiol production through IMP2-dependent mechanisms. This finding provides a new molecular mechanism for PCOS.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Review
Developmental Biology
Kayhan Yakin, Francesko Hela, Ozgur Oktem
Summary: The corpus luteum is the main source of progesterone, and understanding its signaling pathways is crucial for managing reproductive disorders. This review focuses on the complex mechanisms of progesterone-induced signaling in luteal granulosa cell activity and discusses future research priorities and limitations of the current data.
MOLECULAR HUMAN REPRODUCTION
(2023)
Article
Cell Biology
Xuan Dang, Lanlan Fang, Qiongqiong Jia, Ze Wu, Yanjie Guo, Boqun Liu, Jung-Chien Cheng, Ying-Pu Sun
Summary: This study demonstrated that TGF-beta 1 upregulates SPARC expression in the human ovary and the role of SPARC in the pathogenesis of ovarian hyperstimulation syndrome (OHSS). The activation of Slug transcription factor is required for the TGF-beta 1-induced SPARC expression. SPARC affects the occurrence of OHSS by regulating the expression of VEGF and aromatase. These findings are of great significance for the treatment of clinical infertility and OHSS.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Endocrinology & Metabolism
Xiao-lin Jiang, He Tai, Xuan-si Xiao, Shi-yu Zhang, Shi-chao Cui, Shu-bo Qi, Dan-dan Hu, Li-na Zhang, Jin-song Kuang, Xian-sheng Meng, Shun-min Li
Summary: This study found that CFDT can alleviate pathological damage and apoptosis in PCOS rat model. In addition, CFDT can improve ovarian function, reduce inflammatory response, inhibit apoptosis of granular cells, and inhibit the operation of ASK1/JNK pathway in PCOS rats.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Genetics & Heredity
Xiong Yuan, Wenjie Ma, Shuping Chen, Huiyuan Wang, Chenyi Zhong, Li Gao, Yugui Cui, Danhua Pu, Rongrong Tan, Jie Wu
Summary: This study identified a novel CLPP missense variant in a woman with premature ovarian insufficiency (POI), which may contribute to the development of POI.
FRONTIERS IN GENETICS
(2023)
Review
Agriculture, Dairy & Animal Science
J. L. Pate, C. H. K. Hughes
Summary: The corpus luteum plays a critical role in pregnancy in all mammals. However, the fate of the corpus luteum in ruminants depends on the presence of a functional uterus or signals from a developing embryo. Prostaglandin F2alpha, secreted by the uterus, is a key molecule that needs to be modified. The sensitivity of the corpus luteum to prostaglandin F2alpha can be influenced by internal mechanisms. Understanding the molecular changes that occur during different stages of luteal development and pregnancy can provide insight into the regulation of luteolytic capacity in ruminants.
Article
Agriculture, Dairy & Animal Science
Alexander V. Sirotkin, Martina Macejkova, Adam Tarko, Zuzana Fabova, Abdel Halim Harrath
Summary: This study examined the effects of buckwheat, rooibos, and vitex on the female reproductive system and their ability to mitigate the reproductive toxicity of toluene. The findings demonstrate that toluene has direct toxic effects on ovarian cells and that these plant extracts can either promote or mitigate the effects of toluene, with vitex potentially acting as a natural protector against toluene's suppressive effect on female reproduction.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Editorial Material
Endocrinology & Metabolism
Marco Centanni, Camilla Virili
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Obstetrics & Gynecology
Kenji Ezoe, Tetsuya Miki, Hikari Akaike, Kiyoe Shimazaki, Tsubasa Takahashi, Yuko Tanimura, Ayumi Amagai, Ayano Sawado, Mai Mogi, Shigeru Kaneko, Satoshi Ueno, Giovanni Coticchio, Danilo Cimadomo, Andrea Borini, Laura Rienzi, Keiichi Kato
Summary: Maternal ageing significantly affects pronuclear size and intra-and extra-nuclear dynamics during fertilization, dysregulates cell polarity during compaction, and reduces blastocoel expansion.
HUMAN REPRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Silvia Martina Ferrari, Giusy Elia, Francesca Ragusa, Sabrina Rosaria Paparo, Valeria Mazzi, Armando Patrizio, Simona Piaggi, Enke Baldini, Marco Centanni, Concettina La Motta, Alessandro Antonelli, Poupak Fallahi
Summary: This study evaluated the antineoplastic effect of multitarget tyrosine kinase inhibitor pazopanib in primary human anaplastic thyroid cancer cells in vitro. The results showed that pazopanib inhibited cell proliferation, migration, and invasion, and increased apoptosis. Moreover, pazopanib significantly decreased the expression of VEGF in the cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Obstetrics & Gynecology
Giovanni Coticchio, Danilo Cimadomo, Greta Chiara Cermisoni, Laura Rienzi, Enrico Papaleo, Filippo Maria Ubaldi, Andrea Borini, Lucia De Santis
Summary: Human embryos are often affected by maternally inherited aneuploidies, leading to developmental failure at early stages. Recent evidence shows that aberrant patterns at the cellular or molecular level can impact multiple stages of embryo development. Fertilization is a delicate phase where centrosomes are assembled and nuclei undergo crucial changes. Errors in this process can result in cellular or genomic integrity loss and fatal consequences for embryonic development.
HUMAN REPRODUCTION
(2023)
Article
Genetics & Heredity
Alessandro Bartolacci, Andrea Borini, Danilo Cimadomo, Gemma Fabozzi, Roberta Maggiulli, Cristina Lagalla, Daniela Pignataro, Miriam dell'Aquila, Federica Parodi, Gilda Patria, Carlotta Zaca, Filippo Maria Ubaldi, Laura Rienzi, Giovanni Coticchio
Summary: This study investigated whether high relative humidity conditions, when using a time-lapse system and sequential culture media, are beneficial to embryo culture and improve ongoing pregnancy rates. The results suggest that high relative humidity conditions do not improve the rate of ongoing pregnancy and several embryological outcomes, under the conditions used in this study.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Obstetrics & Gynecology
Alessandro Bartolacci, Miriam dell'Aquila, Giovanni Coticchio, Giulia Intra, Federica Parodi, Gilda Patria, Carlotta Zaca, Andrea Borini
Summary: A retrospective analysis of 716 women undergoing their first sIVF cycles was conducted to examine the effect of endometriosis on embryo development and clinical outcomes. The study found that endometriosis does not significantly impact embryo development and live birth, but does affect the number of retrieved oocytes.
Article
Medicine, General & Internal
Danilo Cimadomo, Viviana Chiappetta, Federica Innocenti, Gaia Saturno, Marilena Taggi, Anabella Marconetto, Valentina Casciani, Laura Albricci, Roberta Maggiulli, Giovanni Coticchio, Aisling Ahlstrom, Jorgen Berntsen, Mark Larman, Andrea Borini, Alberto Vaiarelli, Filippo Maria Ubaldi, Laura Rienzi
Summary: Preimplantation genetic testing for aneuploidies (PGT-A) is an effective embryo selection strategy, but it has limitations. The iDAScore v1.0 model, based on artificial intelligence, can objectively evaluate embryo quality and competence. It shows promising performance in predicting embryo morphology and live-birth outcomes, potentially improving embryologists' evaluations.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Oncology
Federica Innocenti, Sara Di Persio, Marilena Taggi, Roberta Maggio, Pina Lardo, Vincenzo Toscano, Rita Canipari, Elena Vicini, Antonio Stigliano
Summary: Mitotane (MTT) is the preferred treatment for adrenocortical carcinoma. This study aimed to evaluate the impact of MTT on male gonadal function and propose a sperm cryopreservation program for male patients of childbearing age. The results showed that MTT negatively affects the male reproductive system, including changes in testicular tissue morphology and reductions in sperm concentration and quality.
Article
Obstetrics & Gynecology
A. Ahlstroem, K. Lundin, D. Cimadomo, G. Coticchio, U. Selleskog, G. Westlander, J. Winerdal, C. Stenfelt, S. Callender, C. Nyberg, M. Astroem, K. Loefdahl, L. Nolte, M. Sundler, M. Kitlinski, I. Liljeqvist Soltic, T. Bohlin, J. Baumgart, K. E. Lindgren, F. Guelen Yaldir, L. Rienzi, A. K. Lind, C. Bergh
Summary: This Swedish study investigated whether embryo culture in a closed time-lapse system is associated with any differences in perinatal and maternal outcomes compared to conventional culture and spontaneous conceptions. The study found no significant differences between time-lapse and conventional incubation systems in terms of preterm birth, low birth weight, and hypertensive disorders of pregnancy. However, singletons born from time-lapse incubation had a higher risk of preterm birth and low birth weight compared to singletons from spontaneous conceptions.
HUMAN REPRODUCTION
(2023)
Article
Obstetrics & Gynecology
Giovanni Coticchio, Kenji Ezoe, Cristina Lagalla, Carlotta Zaca, Andrea Borini, Keiichi Kato
Summary: Comparing embryos reaching the blastocyst stage between Days 4-7, it was found that longer times to blastocyst formation are associated with worse clinical outcomes and disruptions in developmental patterns. These differences emerge as early as the fertilization stage.
HUMAN REPRODUCTION
(2023)
Article
Obstetrics & Gynecology
Viviana Chiappetta, Federica Innocenti, Giovanni Coticchio, Aisling Ahlstrom, Laura Albricci, Vicente Badajoz, Maria Hebles, Miguel Gallardo, Francesca Benini, Stefano Canosa, Jiri Kumpost, Katarina Milton, Diletta Montanino Oliva, Roberta Maggiulli, Laura Rienzi, Danilo Cimadomo
Summary: The study aims to investigate whether embryologists from different European countries agree on the disposition decisions regarding Day 7 and low-quality blastocysts.
HUMAN REPRODUCTION
(2023)
Review
Endocrinology & Metabolism
Camilla Virili, Ilaria Stramazzo, Maria Flavia Bagaglini, Anna Lucia Carretti, Silvia Capriello, Francesco Romanelli, Pierpaolo Trimboli, Marco Centanni
Summary: This systematic review examined the relationship between thyroid pathophysiology and intestinal microbiota composition, and found that the reciprocal influence between these two entities is extensive. However, conflicting results reported in the literature require further clarification on several aspects of this correlation.
REVIEWS IN ENDOCRINE & METABOLIC DISORDERS
(2023)
Article
Nutrition & Dietetics
Vincenzo Spina, Enke Baldini, Silvia Cardarelli, Cosimo Oliva, Stefano Venarubea, Franca Faraoni, Giovanni Pastore, Rachele Tittoni, Angela Musella, Antonia Squarcella, Eleonora Lori, Elisabetta Renzi, Roberta Feroci, Flavia Mastrodonato, Fabiola Ciferri, Camilla Virili, Marco Centanni, Cristina Fabiani, Rocco Rago, Michele Carlo Schiavi, Pierluigi Palazzetti, Eleonora D'Armiento, Vito Cantisani, Salvatore Sorrenti, Salvatore Ulisse
Summary: Adequate iodine intake is crucial in pregnancy, and pregnant women and their fetuses are still exposed to iodine deficiency. The consumption of iodine-containing supplements should be recommended in pregnancy.
Review
Medicine, General & Internal
Poupak Fallahi, Giusy Elia, Francesca Ragusa, Sabrina Rosaria Paparo, Armando Patrizio, Eugenia Balestri, Valeria Mazzi, Salvatore Benvenga, Gilda Varricchi, Laura Gragnani, Chiara Botrini, Enke Baldini, Marco Centanni, Clodoveo Ferri, Alessandro Antonelli, Silvia Martina Ferrari
Summary: The SARS-CoV-2 virus, which causes COVID-19, enters cells through the ACE2 receptor found in various tissues, including endocrine organs like the ovaries, testes, pancreas, and thyroid. SARS-CoV-2 infection has been associated with thyroid disorders and can also induce autoimmune diseases such as Hashimoto's thyroiditis and Graves' disease. Further research is needed to better understand this relationship.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Obstetrics & Gynecology
Pietro Santulli, Christophe Blockeel, Mathilde Bourdon, Giovanni Coticchio, Alison Campbell, Michel De Vos, Kirsten Tryde Macklon, Anja Pinborg, Juan A. Garcia-Velasco
Summary: While there is extensive research on fertility preservation in women with malignant diseases, there is limited research on the role of fertility preservation in non-malignant conditions. This includes various benign gynaecological conditions that may affect reproductive potential. Currently, there is insufficient data to develop guidelines for clinical practice in fertility preservation for women with benign gynaecological conditions.
HUMAN REPRODUCTION OPEN
(2023)