Article
Reproductive Biology
Lei Wang, Yukun Wang, Bijun Li, Yiyu Zhang, Shuaifei Song, Wenfei Ding, Dejun Xu, Zhongquan Zhao
Summary: This study found that bone morphogenetic protein 6 (BMP6) can promote the secretion and expression of anti-Mullerian hormone (AMH) in goat ovarian granulosa cells (GCs). Mechanistically, BMP6 upregulates the expression of sex-determining region Y-box 9 (SOX9) and GATA-binding factor 4 (GATA4), which leads to the transcriptional initiation of AMH.
Article
Reproductive Biology
Rushi H. Patel, Vivien B. Truong, Reem Sabry, Julianna E. Acosta, Kiera Mccahill, Laura A. Favetta
Summary: This study investigated the effects of bisphenols on the anti-Mullerian hormone pathway. The results showed that bisphenol A significantly increased SMAD protein mRNA expression, while bisphenol S was independent of the anti-Mullerian hormone receptor. These findings provide a potential mechanism for bisphenols to disrupt follicular development and decrease oocyte competence.
BIOLOGY OF REPRODUCTION
(2023)
Article
Obstetrics & Gynecology
Samantha Sperduti, Elia Paradiso, Claudia Anzivino, Clara Lazzaretti, Silvia Limoncella, Sara D'Alessandro, Neena Roy, Francesca Reggianini, Tommaso Ferrari, Beatrice Melli, Giovanni Battista La Sala, Alessia Nicoli, Jessica Daolio, Maria Teresa Villani, Simonetta Tagliavini, Tommaso Trenti, Francesco Poti, Reinhild Sandhowe, Chiara Centonze, Monica Lispi, Manuela Simoni, Livio Casarini
Summary: The addition of LH to FSH can recover the cAMP and progesterone production of sub/poor-responder women's ovarian granulosa cells. This study has important implications for personalized ART stimulation protocols.
HUMAN REPRODUCTION
(2023)
Review
Geriatrics & Gerontology
Panagiotis Anagnostis, Panagiota Florou, Julia K. Bosdou, Grigorios F. Grimbizis, Ioannis Iakovou, Efstratios M. Kolibianakis, Dimitrios G. Goulis
Summary: This study reviewed the effect of RAI on ovarian reserve in premenopausal women with DTC, finding that AMH concentrations decreased at 3 months post-treatment and remained low at 6 and 12 months. FSH concentrations showed no difference at these time points.
Article
Endocrinology & Metabolism
Linn Salto Mamsen, Jane Alro Botkjaer, Stine Gry Kristensen, Susanne Elisabeth Pors, Janni Vikkelso Jeppesen, Ajay Kumar, Bhanu Kalra, Erik Ernst, Claus Yding Andersen
Summary: AMH, a member of the TGF-beta superfamily produced by follicular granulosa cells in women, is thought to inhibit aromatase and play a role in ovarian steroidogenesis. This study found a negative correlation between follicle diameter and AMH forms, and variability in AMH isoforms between follicles of identical size within the same woman. The complex intrafollicular processing of AMH may pose challenges for developing an antibody-based AMH assay.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Medicine, Research & Experimental
Ting Liu, Yifei Huang, Hui Lin
Summary: This review article discusses the regulation and mechanism of aromatase activity in ovarian granulosa cells, as well as the impact of aromatase regulation on estrogen secretion. It highlights the influence of endocrine-disrupting substances and other factors on aromatase expression, which can lead to an imbalance in estrogen secretion by target tissues.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Obstetrics & Gynecology
Dana R. Siegel, Laura Grau, Mary Sammel, Liesl Nel-Themaaat, Nanette Santoro, Alex J. Polotsky
Summary: This study aimed to examine whether AMH and FSH can predict live birth. By analyzing a large number of pregnancy records, it was found that AMH and FSH were not associated with the probability of live birth, and the only significant factor associated with live birth was age.
REPRODUCTIVE SCIENCES
(2023)
Article
Biology
Lixian Qin, Chantacha Sitticharoon, Somsin Petyim, Issarawan Keadkraichaiwat, Rungnapa Sririwichitchai, Pailin Maikaew, Malika Churintaraphan
Summary: This study examined the effects of adiponectin on IVF treatment and its influence on gene expression in human granulosa-like tumor cells. The results showed that adiponectin levels did not differ between successful and unsuccessful pregnancies, but high adiponectin levels in serum compared to follicular fluid might negatively impact IVF treatment.
Article
Developmental Biology
V Cadoret, P. Jarrier-Gaillard, P. Papillier, D. Monniaux, F. Guerif, R. Dalbies-Tran
Summary: The cytokine LIF has a modulatory role in the development of sheep in vitro follicles, affecting granulosa cell differentiation and hormone secretion. Combined supplementation with LIF and FSH improves oocyte meiotic competence and may be worth investigating with human follicles.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Agriculture, Dairy & Animal Science
Conghao Zhong, Zhansheng Liu, Dandan Li, Li Kang, Yunliang Jiang
Summary: Follicle selection is a crucial process for chicken laying performance and fecundity. In this study, the impacts of follicle-stimulating hormone (FSH) on chicken granulosa cells were investigated through ONT transcriptome sequencing. FSH treatment upregulated the expression of several genes related to steroid biosynthesis and pathways associated with ovarian steroidogenesis. Specifically, TRAF7 was identified as a key regulator in promoting the expression of steroidogenic enzymes and granulosa cell proliferation. This study provides valuable insights into the molecular mechanism of follicle selection in chickens.
Article
Obstetrics & Gynecology
Joie Z. Guner, Diana Monsivais, Henry Yu, Fabio Stossi, Hannah L. Johnson, William E. Gibbons, Martin M. Matzuk, Stephen Palmer
Summary: TOP5300 appears to stimulate higher E2 production and steroidogenic gene expression in human granulosa cells from patients with polycystic ovary syndrome (PCOS) compared to recombinant human FSH (rh-FSH). FSH receptor (FSHR) membrane localization does not seem to be a limiting step for the stimulation of steroidogenic gene expression and E2 production by TOP5300 or rh-FSH.
FERTILITY AND STERILITY
(2023)
Article
Medicine, Research & Experimental
Xu Li, Gaoqing Xu, Zhiqiang Li, Hongyu Liu, Xin Ma, Lianyu Yang, Pengju Zhang, Jing Zhao, Jun Wang, Wenfa Lu
Summary: Research indicates that GnIH inhibits the proliferation and promotes apoptosis of bovine granulosa cells through the p38 signaling pathway, and the use of a p38 activator can alleviate these effects.
Article
Agriculture, Dairy & Animal Science
Xiaolin Li, Jiapeng Lin, Ying Chen, Liqin Wang, Bing Han, Bin Jia, Yangsheng Wu, Juncheng Huang
Summary: Thrombospondin (TSP1) is widely expressed in sheep ovaries and localized in the cytoplasm and nucleus of granulosa cells. Its expression is regulated by follicular development and follicle-stimulating hormone (FSH). FSH inhibits TSP1 expression and promotes sheep granulosa cell proliferation, while a TSP1 mimetic peptide offsets this proliferation. Inhibitors of the PI3K and ERK pathways promote TSP1 expression and inhibit sheep granulosa cell proliferation.
ANIMAL BIOTECHNOLOGY
(2022)
Review
Endocrinology & Metabolism
Casper P. P. Hagen, Margit Bistrup Fischer, Gylli Mola, Theis Bech Mikkelsen, Line Hartvig Cleemann, Claus Hojbjerg Gravholt, Mette H. H. Viuff, Anders Juul, Anette Tonnes Pedersen, Katharina Maria Main
Summary: Turner syndrome (TS) is a chromosomal disorder characterized by the absence of the second X chromosome, affecting about 1 in 2500 female births. TS is associated with primary ovarian insufficiency (POI), with only a small percentage of women achieving pregnancy without egg donation. This review evaluates the clinical use of ovarian function markers in TS patients and reports on the transition from pediatric to gynecological and adult endocrinological care in a Danish center.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Obstetrics & Gynecology
Hana Visnova, Enrico Papaleo, Fernando Sanchez Martin, Katarzyna Koziol, Bjarke M. Klein, Bernadette Mannaerts
Summary: Individualized follitropin delta dosing provides an improved efficacy-safety balance in women with high ovarian reserve, reducing the risk of ovarian hyperstimulation syndrome (OHSS) without compromising the chance of pregnancy.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Environmental Sciences
Lei Liu, Di Wang, Xingzheng Li, Adeyinka Abiola Adetula, Adnan Khan, Bing Zhang, Huagui Liu, Ying Yu, Qin Chu
Summary: This study investigated the effects of maternal exposure to LPS on the immunity and reproductive performance of laying hens and their offspring. The results showed that LPS maternal stimulation reduced the egg-laying rate of hens and their offspring, with long-lasting effects on gene expression patterns. Further analysis revealed that LPS maternal stimulation mainly influenced the reproduction and immunity of laying hens and their offspring, with specific genes playing essential roles.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Genetics & Heredity
Abdul Sammad, Hailiang Zhang, Rui Shi, Yixin Dong, Hanpeng Luo, Ziwei Chen, Lin Liu, Gang Guo, Aoxing Liu, Yachun Wang
Summary: In this study, the associations and expression levels of three genes, CACNB2, SLC39A12, and ZEB1, with reproduction in dairy cattle were investigated. The results demonstrated the importance of these genes in reproductive traits and provided valuable genetic markers for genomic selection in this area.
FRONTIERS IN GENETICS
(2022)
Article
Agriculture, Dairy & Animal Science
Baigao Yang, Tong Hao, Sha Yang, Haisheng Hao, Weihua Du, Huabin Zhu, Peipei Zhang, Xueming Zhao
Summary: A study investigated the expression patterns and functions of fertilization proteins JUNO and CD9 in bovine oocytes during in vitro maturation (IVM) and in vitro fertilization (IVF). The results showed that the mRNA and protein expression levels of JUNO and CD9 genes increased from bovine GV oocytes to MII oocytes, and similar expression patterns were observed in cumulus cells. Treatment with antibodies against JUNO or CD9 significantly reduced the fertilization rates of oocytes, with JUNO antibody showing a major impact on the fertilization ability of bovine oocytes.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Agronomy
Hailiang Zhang, Abdul Sammad, Rui Shi, Yixin Dong, Shanjiang Zhao, Lin Liu, Gang Guo, Qing Xu, Aoxing Liu, Yachun Wang
Summary: In this study, we investigated the polymorphism effects of two metabolic-syndrome pathway genes, IL6R and LEPR, on reproductive performance in dairy cows. Through population-level association analyses and cell-level gene expression analyses, we found that several single nucleotide polymorphisms in IL6R and LEPR were significantly associated with reproductive traits in Chinese Holstein cows. The functional validation of these genes improved our understanding of cattle reproduction and provided molecular markers for genetic selection of reproductive traits in high-yielding dairy cattle.
Article
Biochemistry & Molecular Biology
Congcong Zhang, Shuhui Wang, Lirong Hu, Hao Fang, Gong Chen, Xiaojuan Ma, Ying Yu, Yachun Wang, Qing Xu
Summary: The study aimed to identify key circRNAs and pathways associated with heat stress in Holstein cows. By comparing milk yield, rectal temperature, and respiratory rate between heat stress and non-heat stress conditions, differences were observed indicating heat stress in cows. RNA-seq analysis revealed differentially expressed circRNAs enriched in multiple signaling pathways. Key circRNAs were identified as potential regulators of heat stress response in cows.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Adnan Khan, Hongying Mi, Fei Gao, Qi Hu, Xia Gu, Fei Ma, LiuHong Qu, Sitao Li, Yiheng Dai, Hu Hao
Summary: Risks associated with preterm birth are unevenly distributed across all gestations, with complications such as NEC and LOS conditions more common at earlier gestational ages. The colonization process of fecal microbiota in preterm infants is highly dynamic at different time points after birth. Certain bacterial groups like Exiguobacterium, Acinetobacter, and Citrobacter decline in abundance with age, while Enterococcus (Klebsiella and Escherichia coli) gradually become the dominant microbiota at 42 days. Bifidobacteria colonization in preterm infants is relatively late, and the presence of the Chryseobacterium bacterial group varies at different time points.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Fan Zhang, Jinhuan Dou, Xiuxin Zhao, Hanpeng Luo, Longgang Ma, Lei Wang, Yachun Wang
Summary: This study exposed rats to high temperature and analyzed their gene expression and hormone levels. Using WGCNA, the study identified key genes Parp2, Mettl3, and Zfp36l1 that are involved in heat stress regulation.
Article
Agriculture, Dairy & Animal Science
Yumei Chen, Zaheer Abbas, Lirong Hu, Ling Kang, Xiao Tan, Qing Xu, Yachun Wang
Summary: This study investigates the effects of mastitis and heat stress on plasma cfDNA levels in dairy cattle. A complete system for the extraction of cell-free DNA from plasma was established using the TIANamp Micro DNA Kit. The results show that cfDNA is related to mastitis and heat stress in dairy cattle.
Article
Biochemistry & Molecular Biology
Peipei Zhang, Baigao Yang, Xi Xu, Hang Zhang, Xiaoyi Feng, Haisheng Hao, Weihua Du, Huabin Zhu, Shujing Li, Wenli Yu, Adnan Khan, Saqib Umer, Xueming Zhao
Summary: Oocyte maturation is crucial for female gametogenesis and subsequent fertilization and embryogenesis. This study investigated the effect of Pre-IVM with CNP, MT, and FLI on the developmental potential of bovine oocytes during in vitro maturation (IVM). The combination of CNP, MT, and FLI significantly improved the development of oocytes to blastocyst stage, as well as ATP content, GSH levels, TZP intensity, Delta psi m, cacline-AM fluorescence intensity, and reduced ROS levels. The survival and hatched rates after vitrification were also significantly higher in the CNP+MT+FLI group. Overall, targeting the combination of CNP, MT, and FLI can enhance the quality and developmental potential of bovine oocytes.
Article
Agriculture, Dairy & Animal Science
Bahlibi Weldegebriall Sahlu, Huan Wang, Zhihui Hu, Nuo Heng, Jianfei Gong, Haoyu Wang, Huabin Zhu, Shanjiang Zhao
Summary: This study constructed a circRNA-miRNA-mRNA regulatory network based on transcriptome profiles of different developmental stages in Holstein bulls, revealing the regulatory role and mechanism of circRNAs in adult testicular spermatogenesis, and further expanding our understanding of the role of circRNAs in spermatogenesis.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Xiuxin Zhao, Hanpeng Luo, Haibo Lu, Longgang Ma, Yanqin Li, Jinhuan Dou, Junxing Zhang, Yun Ma, Jianbin Li, Yachun Wang
Summary: Genetic selection for immune response features is an effective strategy for improving animal health. This study identified differentially expressed genes (DEGs) associated with immune responses in dairy bulls, and the results revealed a relationship between DEGs and locomotion, tissue development, immune response, and detoxification. The KEGG pathway analysis showed that most DEGs were enriched in pathways related to disease, inflammation, and immune response, providing insights for genomic selection.
Article
Agriculture, Dairy & Animal Science
Yuechuan Huang, Hailiang Zhang, Cheng Mei, Minglu Yang, Shanjiang Zhao, Huabin Zhu, Yachun Wang
Summary: This study analyzed the phenotypic and genetic characteristics of in vitro embryo production traits in Chinese Holstein cattle, and investigated the factors influencing the production efficiency of in vitro embryo production. The results showed that the traits were significantly influenced by season and donor age, and exhibited heritability with low to high estimates. The count traits and proportion traits reflected different aspects of embryo production and should be considered in genetic selection to improve efficiency.