Article
Engineering, Biomedical
Axel Poessl, David Hartzke, Thomas M. Schmidts, Frank E. Runkel, Peggy Schlupp
Summary: Bioprinting for tissue or disease models is a complex process involving biofabrication, cell culture and bioink, requiring an artificial ECM to mimic the natural extracellular matrix functions. Through multi-material blending and experimental design approaches, a highly reproducible hydrogel can be developed for printing artificial ECM. The printing behavior and short-term cultivation stability of the bioink can be evaluated by adjusting key process parameters and analyzing rheological measurements.
Article
Materials Science, Multidisciplinary
Yi Zhang, Piyush Kumar, Songwei Lv, Di Xiong, Hongbin Zhao, Zhiqiang Cai, Xiubo Zhao
Summary: 3D bioprinting technology combines computing science, biology, and material engineering, and has great potential in fabricating vascularized constructs for tissue engineering. This review summarizes recent advances in 3D bioprinting of vascularized tissues, including the selection of biomaterials and various 3D printing techniques and applications.
MATERIALS & DESIGN
(2021)
Article
Multidisciplinary Sciences
Shangjing Xin, Kaivalya A. Deo, Jing Dai, Navaneeth Krishna Rajeeva Pandian, David Chimene, Robert M. Moebius, Abhishek Jain, Arum Han, Akhilesh K. Gaharwar, Daniel L. Alge
Summary: An in-depth investigation was conducted on the dissipation process of HMPs during printing, revealing that external resistance and internal physicochemical properties play a key role in the printing outcome. Understanding these mechanisms can help improve the printability of HMPs and enhance their use in 3D bioprinting.
Review
Pharmacology & Pharmacy
Annika C. Dell, Grayson Wagner, Jason Own, John P. Geibel
Summary: 3D bioprinting plays a crucial role in tissue engineering in medicine by providing precise and highly customizable methods for creating biological tissues. The selection of appropriate cell ink formulations is vital for process optimization and customization to suit target tissues. Hydrogel-based cell ink formulations show promise for future development in bioprinting technology.
Article
Biochemistry & Molecular Biology
Nadezhda V. Arguchinskaya, Elena V. Isaeva, Anastas A. Kisel, Evgeny E. Beketov, Tatiana S. Lagoda, Denis S. Baranovskii, Nina D. Yakovleva, Grigory A. Demyashkin, Liudmila N. Komarova, Svetlana O. Astakhina, Nikolai E. Shubin, Peter V. Shegay, Sergey A. Ivanov, Andrey D. Kaprin
Summary: Gelatin methacryloyl (GelMA) has attracted attention due to its tunable mechanical properties. This study synthesized highly substituted GelMA and investigated its suitability for 3D printing, cytocompatibility, and biocompatibility. The results showed that a 15% concentration GelMA achieved satisfactory printing quality, but the high degree of substitution decreased the adhesion ability of human adipose-derived stem cells (ADSCs) to the GelMA surface. In animal implantation, GelMA degradation was slowed down.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Mitsuyuki Hidaka, Masaru Kojima, Masaki Nakahata, Shinji Sakai
Summary: This study demonstrates the significant potential of using visible light-curable inks containing chitosan derivatives for extrusion and vat polymerization-based bioprinting towards biomedical applications. By adjusting the concentrations of SPS and Ru(bpy)(3), the gelation time of the solution and the mechanical properties of the resultant hydrogels can be altered, resulting in 3D hydrogel constructs with good shape fidelity, biodegradability, and antimicrobial activity.
Article
Engineering, Biomedical
Margaret E. Prendergast, Jason A. Burdick
Summary: The study examines the characteristics of hydrogel inks in suspension bath printing through rheological, computational, and experimental analyses, emphasizing the importance of key rheological properties and print parameters in the printing process.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Computer Science, Artificial Intelligence
Xi Huang, Wei Long Ng, Wai Yee Yeong
Summary: In this study, a machine learning approach was used to predict the number of cells within printed droplets based on droplet velocity at two different points along the nozzle-substrate distance. A contactless method combining an optical system and machine learning algorithms was employed for cell detection within single droplets and prediction of the total number of printed cells within multiple droplets. Various machine learning algorithms were compared, and the random forest regressor algorithm achieved the highest accuracy in cell prediction within single droplets and the extra tree regressor algorithm demonstrated the lowest mean error in predicting the number of printed cells within multiple droplets. Incorporating these models in a droplet monitoring system enables live assessment of the number of printed cells during an inkjet-based bioprinting process.
JOURNAL OF INTELLIGENT MANUFACTURING
(2023)
Article
Engineering, Biomedical
Ana Leticia Rodrigues Costa, Stephanie M. Willerth, Lucimara Gaziola de la Torre, Sang Won Han
Summary: Ischemia occurs when blood flow is reduced or restricted, leading to a lack of oxygen and nutrient supply and removal of metabolites. Critical limb ischemia (CLI) is a severe manifestation of peripheral arterial disease, primarily caused by atherosclerosis. Current treatments for CLI are not very effective, and some patients cannot undergo vascular surgery due to physiological differences. Gene and cell therapies are emerging as alternative treatments for CLI, and encapsulating cells in hydrogels for local delivery shows promise.
MATERIALS TODAY BIO
(2022)
Article
Materials Science, Multidisciplinary
Tao Zhang, Wei Zhao, Zijie Xiahou, Xingwang Wang, Kunxi Zhang, Jingbo Yin
Summary: Extrusion-based bioprinting is the most important method for 3D bioprinting, creating living tissues and organs by stacking cells and materials layer by layer. Bioink plays a crucial role in this process, determining cell survival, growth, and organ precision.
APPLIED MATERIALS TODAY
(2021)
Article
Engineering, Biomedical
Wei Long Ng, Xi Huang, Viktor Shkolnikov, Guo Liang Goh, Ratima Suntornnond, Wai Yee Yeong
Summary: A study utilizing a thermal inkjet system to dispense sub-nanoliter cell-laden droplets identified droplet impact velocity and droplet volume as significant factors affecting the viability and proliferation of printed cells. Increasing cell concentration leads to slower impact velocity, improving cell viability, while a minimum droplet volume of 20 nL helps mitigate evaporation-induced cell damage. Control of droplet impact velocity and volume is crucial for viability and proliferation of printed human primary cells in sub-nanoliter bioprinting.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2022)
Review
Polymer Science
Helena Herrada-Manchon, Manuel Alejandro Fernandez, Enrique Aguilar
Summary: Rheology is crucial in extrusion-based 3D printing, especially in the field of hydrogels. The rheological properties of hydrogels, such as shear thinning behavior and viscoelasticity, significantly impact the printing process and result quality. This review paper emphasizes the importance of understanding rheology in optimizing hydrogel-based 3D printing and achieving desired outcomes.
Article
Materials Science, Ceramics
Soumitra Das, Bikramjit Basu
Summary: The particle shape and size distribution of inorganic fillers are important in scaffold buildability when combined with viscoelastic polymers. By incorporating rod-shaped nanocrystalline HAp powders of varying sizes and shapes into GelMA hydrogel matrix, the mechanical properties of the composite hydrogel scaffolds were improved while water uptake rate and enzymatic degradation were reduced. The inorganic-organic nanocomposite hydrogel could be a potential bioink for 3D bioprinting applications in tissue regeneration.
INTERNATIONAL JOURNAL OF APPLIED CERAMIC TECHNOLOGY
(2022)
Article
Polymer Science
Giada Loi, Franca Scocozza, Flaminia Aliberti, Lorenza Rinvenuto, Gianluca Cidonio, Nicola Marchesi, Laura Benedetti, Gabriele Ceccarelli, Michele Conti
Summary: Cells are influenced by various biomechanical aspects of their microenvironment, such as substrate geometry. The effect of substrate geometry on muscle cell behavior, particularly in terms of cell proliferation, is known. However, its influence on the myogenic differentiation process is not clear and requires further investigation.
Article
Engineering, Biomedical
Jesus M. Rodriguez-Rego, Laura Mendoza-Cerezo, Antonio Macias-Garcia, Alfonso C. Marcos-Romero, Juan P. Carrasco-Amador
Summary: Additive manufacturing technologies have many medical applications, mainly due to advancements in the development of materials used for customized models. However, obtaining correct cell viability values remains a challenge, which is why three-dimensional bioprinting is a valuable tool that requires extensive study. In this work, the comparison of 3D bioprinting technology using hydrogels and thermoplastics for biomimetic parts development is proposed. The goal is to study the printability of different materials and determine their ability to produce biomimetic structures with structural guarantees. Gelatin methacryloyl (GelMA) 5% was found to be the only material capable of generating biomimetic structures faithful to the designed 3D model. Additionally, it is shown that further development of hydrogels is needed to match the results achieved by thermoplastic materials in bioprinting.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)