Journal
BIOTECHNIQUES
Volume 58, Issue 3, Pages 103-119Publisher
FUTURE SCI LTD
DOI: 10.2144/000114262
Keywords
protein complex; protein purification; affinity; proteomics; interactomics
Funding
- Empire State Stem Cell fund through NYSDOH [C023046]
- NIH [P41 GM109824, 1P50GM107632-01, P41 GM103314]
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Dissecting and studying cellular systems requires the ability to specifically isolate distinct proteins along with the co-assembled constituents of their associated complexes. Affinity capture techniques leverage high affinity, high specificity reagents to target and capture proteins of interest along with specifically associated proteins from cell extracts. Affinity capture coupled to mass spectrometry (MS)-based proteomic analyses has enabled the isolation and characterization of a wide range of endogenous protein complexes. Here, we outline effective procedures for the affinity capture of protein complexes, highlighting best practices and common pitfalls.
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