Article
Dermatology
Jiacheng Dong, Guanzhao Liang, Hailin Zheng, Siyue Kan, Nana Song, Meijie Zhang, Weida Liu
Summary: Ravuconazole showed good activity against C. auris and vaginal Candida isolates, suggesting it could be used as an effective treatment for drug-resistant candidiasis.
Article
Microbiology
Jack D. Sobel, Robert Akins
Summary: Antifungal drug susceptibility tests (AST) are increasingly needed for women with refractory or recurrent Candida vaginitis due to fluconazole resistance. It is proposed that the AST be performed at pH 4.5 instead of the recommended pH 7.0, as testing at only pH 7.0 is likely to miss a significant number of clinically relevant azole-resistant C. albicans vaginal isolates.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Aina Mesquida, Teresa Vicente, Elena Reigadas, Maria Palomo, Carlos Sanchez-Carrillo, Patricia Munoz, Jesus Guinea, Pilar Escribano
Summary: The study demonstrated that Ibrexafungerp has good in vitro activity against Candida albicans from vaginal samples, including isolates with reduced susceptibility to fluconazole. Population structure analysis revealed two genotype populations, with some genotypes showing reduced susceptibility to fluconazole.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Review
Infectious Diseases
Rashi Verma, Dibyabhaba Pradhan, Ziaul Hasan, Harpreet Singh, Arun Kumar Jain, Luqman Ahmad Khan
Summary: This study presents the distribution and antifungal resistance patterns of Candida species among the Indian population, which could assist in forming treatment recommendations for the region and beyond in the future.
Review
Immunology
Sarah E. Murphy, Tihana Bicanic
Summary: This review presents current antifungal drugs for Candida infections, discusses mechanisms of resistance, introduces novel antifungal agents under development, and explores the use of combination therapy as a potential strategy against emerging drug-resistant Candida.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Infectious Diseases
Angela Fernandes, Nuno Azevedo, Andreia Valente, Marisol Dias, Ana Gomes, Cristina Nogueira-Silva, Mariana Henriques, Sonia Silva, Bruna Goncalves
Summary: This study highlights the importance of understanding the epidemiology, microbiology, antifungal susceptibility, and risk factors of VVC, in order to improve prevention and treatment strategies for this globally significant infection.
Article
Microbiology
Yisheng Chen, Yongqin Wu, Kaiyi Lulou, Dongting Yao, Chunmei Ying
Summary: This study investigated the antifungal susceptibility and molecular epidemiology of vaginal and non-vaginal Candida glabrata isolates. The results showed high resistance rates to fluconazole, itraconazole, and voriconazole, while all isolates remained susceptible to amphotericin B. Genotyping revealed partially shared genotypes between vaginal and non-vaginal samples, with additional genotypes found in vaginal samples. The majority of resistant strains correlated with the predominant genotype.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Jose Manuel Noguera Bazan, Vitor Lopes Chagas, Raphael Guedes Silva, Izadora Souza Soeiro Silva, Joao Guilherme Nantes Araujo, Guilherme Nantes Araujo, Lucas dos Santos Silva, Karla Lilian Rodrigues Batista, Romerio Rodrigues dos Santos Silva, Maria Tereza dos Santos Correia, Joicy Cortez de Sa Sousa, Cristina de Andrade Monteiro, Aryane Tofanello, Wanius Garcia, Edilausson Moreno Carvalho, Claudener Souza Teixeira, Luis Claudio Nascimento da Silva
Summary: This study characterized alginate-derived bioadhesive films containing the lectins ConBr and MaL and evaluated their anti-infective properties. The results showed that both lectins had prophylactic effects against Candida albicans infection, with MaL significantly increasing larval survival in treatment assays. The alginate films loaded with lectins showed similar micromorphology with slightly irregular surfaces, and the lectins were released in similar patterns. The films exhibited two-step weight loss patterns in TGA analysis. The ConBr-loaded films showed the highest maximum detachment force in the mucoadhesive assay. Overall, the lectins did not induce significant changes in the physicochemical and mechanical characteristics of the alginate films. This study provides the first evidence of the in vivo anti-infective effects of ConBr and MaL against C. albicans and suggests that the lectin-containing alginate films have potential applications in the oral cavity.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Review
Microbiology
Yahaya Hassan, Shu Yih Chew, Leslie Thian Lung Than
Summary: Candida glabrata is an important pathogen that can survive and cause infections in patients using various virulence factors. Factors including adherence, evasion of immune defenses, production of hydrolytic enzymes contribute to infection initiation, as well as iron regulation and genetic mutations. Other factors such as biofilm production, stress tolerance, resistance to neutrophil killings, and development of drug resistance also play a role in the pathogenicity of C. glabrata.
Article
Microbiology
Lamiaa A. Al-Madboly, Mohamed A. Abd El-Salam, Jairo K. Bastos, Safinaz H. El-Shorbagy, Rasha M. El-Morsi
Summary: Our study demonstrates that GQAs can be used as safe and highly effective phytochemicals against multidrug-resistant (MDR) Candida albicans. GQAs exhibited strong antifungal activity by interfering with fungal growth, disrupting fungal membrane integrity, and inducing apoptosis. Both in vitro and in vivo models confirmed the potential therapeutic use of GQAs for the treatment of vaginal candidiasis.
MICROBIOLOGY SPECTRUM
(2022)
Article
Instruments & Instrumentation
Veronica Ferrari Cervi, Camila Parcianello Saccol, Ticiane da Rosa Pinheiro, Roberto Christ Vianna Santos, Marcel Henrique Marcondes Sari, Leticia Cruz
Summary: Nanocapsule-loaded Pullulan films for the treatment of vulvovaginal candidiasis demonstrated promising stability, controlled drug release, and antifungal activity against Candida albicans.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2022)
Article
Mycology
Siyue Kan, Nana Song, Qiuyu Pang, Huan Mei, Hailin Zheng, Dongmei Li, Fan Cui, Guixia Lv, Ruifang An, Ping Li, Zhengai Xiong, Shangrong Fan, Mengzhen Zhang, Yanhong Chen, Qiao Qiao, Xudong Liang, Manhua Cui, Dongyan Li, Qinping Liao, Xiaofang Li, Weida Liu
Summary: Vulvovaginal candidiasis is a global public health issue, and the optimal treatment for recurrent VVC has not been established. In vitro antifungal susceptibility profiling of VVC isolates from different regions of China can provide significant improvements for the control and treatment of this disease.
Article
Microbiology
Xin Yee Ang, Uma Mageswary Mageswaran, Yi Li Fiona Chung, Boon Kiat Lee, Siti Nur Afiqah Azhar, Nurhanis Syazni Roslan, Ili Farhana Binti Saufian, Nor Sheila Mustaffa, Ermadina Mohamad Kalam, Aini Farhah Ibrahim, Normala Abdul Wahid, Zakuan Zainy Deris, Chern-Ein Oon, Wan Fadhlina Wan Adnan, Salina Sany, Min-Tze Liong
Summary: This study found that probiotics have beneficial effects on pregnant women with vaginal candidiasis, including improving symptoms, reducing the abundance of Candida species, increasing the abundance of Lactobacillus species, and shortening the inflammation period.
Article
Biochemistry & Molecular Biology
V. Poorna Pushkala, S. Mary Princess Sulekha, S. Mathukumar, B. Ragavi, U. Sowmiya
Summary: This study investigated the antifungal activity of phytochemicals present in the Siddha formulation Parangipattai Chooranam against the SAP enzyme. The results showed that compounds such as beta-sitosterol, afzelin, apigenin, quercetin and rosmarinic acid demonstrated potential binding affinity with the target amino acids and outperformed the standard drug fluconazole. These findings suggest that the phytochemicals in Parangipattai Chooranam may be an excellent drug choice for the clinical management of vaginal candidiasis.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Michal Smolenski, Susanne Muschert, Dorota Haznar-Garbacz, Katarzyna Malolepsza-Jarmolowska
Summary: Vaginal candidiasis is a global health issue that affects millions of women worldwide. This study developed a nanoemulsion as a potential carrier for the treatment of vaginal candidiasis. The nanoemulsion showed stability during storage and had promising drug release properties. However, further development and standardized testing protocols are needed.
Article
Microbiology
Bruna Nakanishi Fortes, Gaby Scheunemann, Analy Salles de Azevedo Melo, Kelly Ishida
Summary: This study evaluated the effect of antimicrobials on single and mixed biofilms of Aspergillus fumigatus and Pseudomonas aeruginosa. The results showed that Polymyxin B had the best action on Pseudomonas aeruginosa biofilms, while Amphotericin B and caspofungin were the most effective antifungals for inhibiting Aspergillus fumigatus biofilms. Caspofungin also significantly reduced the viability of Pseudomonas aeruginosa in mixed biofilms. Microscopy confirmed changes in hyphae morphology and an increase in fungal and bacterial death cells after combined therapy of mixed biofilms.
RESEARCH IN MICROBIOLOGY
(2023)
Article
Chemistry, Medicinal
Giovanna Cassone Salata, Luciana B. Lopes
Summary: This study investigated the intraductal delivery and localized treatment of breast cancer using hyaluronic acid-modified nanoemulsions that incorporate the cytotoxic agent paclitaxel and the P-glycoprotein inhibitor elacridar. The results showed that the incorporation of perillyl alcohol or tributyrin as components of the nanoemulsion oil phase increased cytotoxicity in MCF-7 cells. However, perillyl alcohol reduced nanoemulsion stability in the presence of the drugs. The nanoemulsion containing both paclitaxel and elacridar in HA and tributyrin showed increased cytotoxicity and reduced IC50 in both MCF-7 and MDA-MB-231 cells. In vivo experiments demonstrated the importance of HA in improving the retention of the nanoemulsion. Overall, this study highlights the potential applicability of the nanoemulsion for localized breast cancer management.
Article
Pharmacology & Pharmacy
Nataly Paredes da Rocha, Aline de Souza, Megumi Nishitani Yukuyama, Thayna Lopes Barreto, Luiza de O. Macedo, Raimar Lobenberg, Gabriel Lima Barros de Araujo, Kelly Ishida, Nadia Araci Bou-Chacra
Summary: This study successfully prepared a DAP nanosuspension and optimized the nanocrystals to achieve good physical stability and bioavailability. The nanosuspension has potential for commercial use in treating infectious and inflammatory diseases.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Julia Sapienza Passos, Vanessa F. M. C. Dartora, Giovanna Cassone Salata, Isabella Draszesski Malago, Luciana B. Lopes
Summary: Breast cancer is a heterogeneous disease that requires personalized treatment strategies. Intraductal administration of drugs offers a minimally invasive way to distribute drugs throughout the ductal tree. Nanostructured systems can enhance the benefits of intraductal administration by improving targeting and selectivity, prolonging tissue retention, and reducing frequency of administration.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Microbiology
Daniel Felipe Freitas De Jesus, Aline Luiza Duarte De Freitas, Isadora Maria De Oliveira, Larissa Costa De Almeida, Rafael Wesley Bastos, Cristina de Castro Spadari, Analy Sales de Azevedo Melo, Daniel de Assis Santos, Leticia Veras Costa-Lotufo, Flavia C. G. Reis, Marcio L. Rodrigues, Helio Alexandre Stefani, Kelly Ishida
Summary: Studies have shown that chalcogen compounds containing selenium have promising antifungal activity against pathogenic species, which can be used as a treatment for cryptococcosis.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Cristina C. Spadari, Dylan M. Lanser, Marcelo Araujo, Daniel F. F. De Jesus, Luciana B. Lopes, Angie Gelli, Kelly Ishida
Summary: Alginate nanoparticles functionalized with polysorbate 80 were developed as carriers for miltefosine in the oral treatment of cryptococcal meningitis. The functionalized nanoparticles reduced infection across the blood-brain barrier, decreased drug cytotoxicity and haemolysis, and improved drug distribution in the brain. In a mouse model, oral treatment with these nanoparticles significantly reduced fungal burden in the brain and lungs without causing toxicity.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Lais Fernanda de Almeida Spoladori, Gabriella Maria Andriani, Isabela Madeira de Castro, Helena Tiemi Suzukawa, Ana Carolina Ramos Gimenes, Guilherme Bartolomeu-Goncalves, Kelly Ishida, Gerson Nakazato, Phileno Pinge-Filho, Rayanne Regina Beltrame Machado, Celso Vataru Nakamura, Galdino Andrade, Eliandro Reis Tavares, Lucy Megumi Yamauchi, Sueli Fumie Yamada-Ogatta
Summary: Candida auris is a drug-resistant fungal pathogen that causes potentially fatal infections in humans. This study found that the metabolites of Pseudomonas aeruginosa LV strain, combined with biologically synthesized silver nanoparticles, showed synergistic fungicidal activity against C. auris, leading to changes in fungal morphology and ultrastructure. These findings suggest a new strategy for controlling C. auris infections.
Article
Biotechnology & Applied Microbiology
Danielle Santos-Lima, Cristina de Castro Spadari, Vinicius de Morais Barroso, Juliana C. S. Carvalho, Larissa Costa de Almeida, Felipe Santiago Chambergo Alcalde, Marcelo Jose Pena Ferreira, Miriam Sannomiya, Kelly Ishida
Summary: This study isolated and identified a lipopeptide-producing bacterium from a bacterial strain, which showed significant antifungal effects and potential for pharmaceutical development. The lipopeptides exhibited excellent performance in inhibiting fungal growth, disrupting fungal biofilms, and inducing morphological changes, making them promising antifungal agents.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Claudio Fukumori, Paola Cristina Branco, Thayna Barreto, Kelly Ishida, Luciana B. Lopes
Summary: This study investigated the parameters and composition of new multiple nanoemulsions (MN) for the oral co-delivery of 5-fluorouracil (5FU) and short-chain triglycerides (SCT) to improve the limited chemotherapy options for colorectal cancer (CRC). The formation of microemulsion was enhanced by mixing monocaprylin with tricaprylin as the oil phase, and the addition of SCT reduced this value. Sodium alginate aqueous dispersion as the internal aqueous phase increased the microemulsion viscosity. The MNs obtained by diluting selected microemulsions in an external aqueous phase showed improved stability and reduced tumor spheroid viability.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Review
Mycology
Cristina de Castro Spadari, Luana Pereira Borba-Santos, Sonia Rozental, Kelly Ishida
Summary: Fungal infections are a global health problem, and current antifungal drugs have limitations. This review discusses the potential of miltefosine (MFS) as a repositioned drug for antifungal treatment, including its mechanisms of action, case reports, and nanocarrier-mediated delivery. Although promising in vitro, there is a lack of data on MFS's antifungal activity in vertebrate animal models and clinical trials, necessitating further research.
JOURNAL DE MYCOLOGIE MEDICALE
(2023)
Review
Infectious Diseases
Murilo Moreira dos Santos, Kelly Ishida
Summary: Candida tropicalis is a notable species in the Candida genus, with a significant epidemiology in tropical regions. It has shown worrying antifungal resistance and unique biology compared to Candida albicans. Understanding its virulence factors and infectious process is crucial for further research.
Article
Biochemical Research Methods
Tim Kirkman, Alice Sketcher, Vinicius de Morais Barroso, Kelly Ishida, Manuela Tosin, Marcio Vinicius Bertacine Dias
Summary: Candida auris is a global health problem with rapid nosocomial transmission and high mortality. The limited availability of antifungal therapy due to widespread resistance and increasing resistance to frontline drugs calls for urgent development of new treatments.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2023)