Journal
CURRENT DRUG DELIVERY
Volume 17, Issue 3, Pages 186-194Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1567201817666200122163251
Keywords
Exosomes; in vivo delivery; administration route; efficiency; intraperitoneal injection; intravenous injection
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Funding
- NSFC [81671690, 81370275, 31771507]
- National Postdoctoral Program for Innovative Talents [BX20180377]
- Major Clinical Renovation Project, Tangdu Hospital [2013LCYJO03]
- Innovation and Development Foundation for Science & Technology, Tangdu Hospital [2017LCYJO07]
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Background: Exosomes are cell-derived nanovesicles that play vital roles in intercellular communication. Recently, exosomes are recognized as promising drug delivery vehicles. Up till now, how the in vivo distribution of exosomes is affected by different administration routes has not been fully understood. Methods: In the present study, in vivo distribution of exosomes following intravenous and intraperitoneal injection approaches was systemically analyzed by tracking the fluorescence-labeled exosomes and ciPCR analysis of C. elegans specific miRNA abundance delivered by exosomes in different organs. Results: The results showed that exosomes administered through tail vein were mostly taken up by the liver, spleen and lungs while exosomes injected intraperitoneally were more dispersedly distributed. Besides the liver, spleen, and lungs, intraperitoneal injection effectively delivered exosomes into the visceral adipose tissue, making it a promising strategy for obesity therapy. Moreover, the results from fluorescence tracking and OCR were slightly different, which could be explained by systemic errors. Conclusion: Together, our study reveals that different administration routes cause a significant differential in vivo distribution of exosomes, suggesting that optimization of the delivery route is prerequisite to obtain rational delivery efficiency in detailed organs.
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