Review
Chemistry, Analytical
Susanna Della Posta, Valeria Gallo, Alessandra Gentili, Chiara Fanali
Summary: The need to reduce the ecological impact of waste in the extraction and analysis processes has led to the use of alternative green solvents. Deep eutectic solvents (DESs) have shown superior extraction capabilities for bioactive molecules, but have difficulties in removal from extracted mixtures. This review provides an overview and critical evaluation of methods for recovering bioactive molecules from extracts obtained using DESs and natural deep eutectic solvents (NADESs).
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Green & Sustainable Science & Technology
Vikash Chandra Roy, Truc Cong Ho, Hee-Jeong Lee, Jin-Seok Park, Seung Yun Nam, Hoyeol Lee, Adane Tilahun Getachew, Byung-Soo Chun
Summary: This study focuses on extracting astaxanthin from shrimp wastes using ultrasound-assisted natural deep eutectic solvents and utilizing the extracts for biodegradable packaging. The optimized ASX-NaDES extract serves as a plasticizer for chitosan-based films, exhibiting superior radical scavenging activities and other physical properties. The use of UAE NaDES for ASX extraction and subsequent incorporation into biodegradable films shows potential for cleaner production.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Chemistry, Multidisciplinary
Yunxuan Wang, Kwang Ho Kim, Keunhong Jeong, Nak-Kyoon Kim, Chang Geun Yoo
Summary: Renewable deep eutectic solvents (DESs) are introduced as effective and eco-friendly solvents and catalysts for lignocellulosic biomass utilization, highlighting their advancements in synthesis and applications. The review also discusses recent biomass pretreatment and fractionation processes using DESs, along with valorization approaches of carbohydrates and lignin. A closed-loop strategy with renewable DESs for achieving a circular bioeconomy is emphasized for sustainable biorefinery development.
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2021)
Article
Food Science & Technology
Sinan Bayram, Naciye Kutlu, Yusuf Can Gercek, Saffet Celik, Nesrin Ecem Bayram
Summary: This study investigated the effects of ten different deep eutectic solvents (DESs) on the extraction of bioactive components from Hypericum perforatum. It was found that DES2 and DES7 showed higher extraction efficiency for phenolics and organic acids, respectively. DESs prepared using organic acid as hydrogen bond donor exhibited antibacterial activity against most bacteria samples, but had a weaker inhibitory effect on yeast-like fungi.
Article
Food Science & Technology
Hugo Duarte, Valentim Gomes, Maria Jose Aliano-Gonzalez, Leonor Faleiro, Anabela Romano, Bruno Medronho
Summary: This study proposes the production of polyphenolic extracts from maritime pine forest residues using an ultrasound-assisted approach. The optimized conditions include a solid-to-solvent ratio of 0.1 g in 10 mL of LA:FA (70:30%, v/v) with no water, 0 M H2SO4, and extraction at 30°C for 40 minutes with an ultrasound amplitude of 80% at 37 kHz. The polyphenol-enriched extracts demonstrate significant antioxidant and antimicrobial activities. This research presents an important alternative for obtaining valuable polyphenolic extracts from discarded forest residues.
Article
Engineering, Chemical
Liliana A. Rodrigues, Ines C. Leonardo, Frederic B. Gaspar, Luisa Cristina Roseiro, Ana Rita C. Duarte, Ana A. Matias, Alexandre Paiva
Summary: The study shows that using DES for extraction can achieve similar protein extraction yields as water, but DES extracts exhibit better antioxidant and antimicrobial potential. Additionally, DES also demonstrate antimicrobial potential, which may enhance the bioactivity of the extracts.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Engineering, Chemical
Rongqing Zhang, Haifeng Chen, Qiang Yu, Yu Zhang, Fen Liu, Fan Wang, Xiaoyan Chen, Yunyun Liu
Summary: The recycling of bioactive compounds from Chinese herb residue is hindered by low extraction efficiency and high energy environmental impact. A microwave-assisted deep eutectic solvent (DES) extraction method was developed to enhance the extraction yield of bioactive compounds from cinnamon twigs residue. The optimal DES system of choline chloride/ethylene glycol achieved a significant increase in extraction yield, with a 41.528% and 41.443% improvement for cinnamic acid and cinnamic aldehyde, respectively, compared to traditional extraction processes. The extraction process was found to be a spontaneous, exothermic, and irreversible reaction, following a second-order kinetic model. Quantum chemistry analysis revealed that the extraction capacity relied mainly on hydrogen bonding force, and the difference in binding energy between the solvent and the target compounds explained the variation in extraction yields.
CHEMICAL ENGINEERING SCIENCE
(2023)
Article
Agricultural Engineering
Belinda Soares, Andre M. da Costa Lopes, Armando J. D. Silvestre, Paula C. Rodrigues Pinto, Carmen S. R. Freire, Joao A. P. Coutinho
Summary: The potential of deep eutectic solvents (DES) as green solvents for Eucalyptus wood delignification was explored, revealing that the aqueous solution of propionic acid:urea is the most effective system for lignin extraction while preserving cellulose fibers.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Review
Agricultural Engineering
Alvaro Lobato-Rodriguez, Beatriz Gullon, Aloia Romani, Pedro Ferreira-Santos, Gil Garrote, Pablo G. Del-Rio
Summary: This review provides a comprehensive overview of the recent advances in the use of deep eutectic solvent pretreatment for lignocellulose fractionation, with a focus on lignin recovery. It discusses the key parameters influencing the process and explores various strategies to enhance its efficiency. The challenges and opportunities associated with the utilization of extraction-derived streams are described, along with recent advancements in solvent recovery techniques. Furthermore, the utilization of computational models for process design and optimization is introduced. Overall, this review serves as a foundation for further research on the integration of deep eutectic pretreatment in sustainable multi-product biorefinery schemes.
BIORESOURCE TECHNOLOGY
(2023)
Article
Green & Sustainable Science & Technology
Olga Dlugosz, Michal Ochnik, Marta Sochocka, Dominika Franz, Beata Orzechowska, Chmielowiec-Korzeniowska Anna, Drabik Agata, Marcin Banach
Summary: In this study, stable selenium sulphide nanoparticles were successfully synthesized, and they exhibited high bioactivity and antiviral activity against microorganisms. The selenium sulphide nanoparticles obtained in the curcumin extract showed strong inhibition against pathogenic fungi and bacteria. These findings have potential implications for the development of novel antimicrobial and antiviral agents.
SUSTAINABLE MATERIALS AND TECHNOLOGIES
(2022)
Review
Chemistry, Multidisciplinary
Lamia Nakhle, Miriana Kfoury, Isabelle Mallard, David Landy, Helene Greige-Gerges
Summary: Analytical scientists are developing sustainable methods for analyzing environmental samples by replacing toxic solvents with safer deep eutectic solvents, which have shown higher efficiency and recovery rates in extractions compared to conventional organic solvents. The efficiency of extraction often depends on the physicochemical properties of deep eutectic solvents.
ENVIRONMENTAL CHEMISTRY LETTERS
(2021)
Article
Multidisciplinary Sciences
Marcelina Mazur, Tomasz Janeczko
Summary: This work presents the method and results of chemo-enzymatic Baeyer-Villiger oxidation of alpha-benzylcyclopentanones in ester solvents and deep eutectic solvents (DES).
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Chemical
Diego Artigas-Hernandez, Alejandro Berzosa, Diederich Aguilar-Machado, Javier Raso, Manuela Artal
Summary: This study evaluated the use of pulse electric fields (PEFs) as a fresh biomass pretreatment method and eutectic mixtures (ESs) as eco-friendly solvents for extracting astaxanthin (AST) from freeze-dried yeast. The results showed that PEF treatment and subsequent incubation stage had a positive effect on the extraction efficiency, with the thymol/salol system being the most effective solvent. The study also found a synergistic effect between AST and thymol. Overall, consecutively using PEFs and ESs allowed for a high extraction efficiency of AST from dry yeast.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Chemistry, Applied
Walter Pitacco, Chiara Samori, Laura Pezzolesi, Virginia Gori, Antonio Grillo, Matteo Tiecco, Martina Vagnoni, Paola Galletti
Summary: Three novel hydrophobic deep eutectic solvents (DESs) based on oleic acid and terpenes were prepared and used to extract astaxanthin. The DESs showed high efficiency in extracting astaxanthin and their chemical composition was safe and edible. The carotenoid profile obtained using DESs was similar to that obtained using traditional organic solvents. The thymol:oleic acid DES (TAO) exhibited superior antioxidant properties and could preserve astaxanthin content after light exposure, making it a promising formulation for the food ingredients/additives industry.
Review
Agricultural Engineering
Maria del Mar Contreras-Gamez, Angel Galan-Martin, Nalin Seixas, Andre M. da Costa Lopes, Armando Silvestre, Eulogio Castro
Summary: Pretreatment processes are crucial for efficient biomass refining, and in the past two decades deep eutectic solvents (DES) have emerged as an alternative, offering more sustainable processes with both environmental and economic benefits. This paper provides an updated review of DES-based applications in biorefinery schemes, discussing their composition, synthesis, recycling, and existing techno-economic and life cycle assessment studies. Challenges, barriers, and perspectives for the scale-up of DES-based processes are also discussed.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Nemanja Teslic, Filipa Santos, Filipe Oliveira, Alena Stupar, Milica Pojic, Anamarija Mandic, Branimir Pavlic, Aleksandra Cvetanovic Kljakic, Ana Rita C. Duarte, Alexandre Paiva, Aleksandra Misan
Summary: Defatted raspberry seeds were used to extract antioxidants and ellagic acid using Natural deep eutectic solvents (NADES). The optimized NADES raspberry extracts exhibited higher antioxidant activity and EA content.
Article
Biochemistry & Molecular Biology
Amit Kumar Halder, Reza Haghbakhsh, Iuliia V. Voroshylova, Ana Rita C. Duarte, Maria Natalia D. S. Cordeiro
Summary: This study presents an evaluation of Quantitative Structure-Property Relationships (QSPR) modeling for predicting the surface tension of deep eutectic solvents (DES). The most predictive model identified provides valuable information about the structural and physicochemical features responsible for DES surface tension. The results guide future DES design and screening, and offer strategies for constructing in silico binary mixture models.
Article
Thermodynamics
Ahmadreza Roosta, Reza Haghbakhsh, Ana Rita C. Duarte, Sona Raeissi
Summary: This study collected 1410 density data points of 166 different deep eutectic solvents (DESs) and used machine learning models and the group contribution (GC) method to predict their densities. The MLPANN-GC and LSSVM-GC models were found to be more accurate than the current correlations and GC models, making them suitable for industrial and academic applications.
FLUID PHASE EQUILIBRIA
(2023)
Article
Genetics & Heredity
Ines Carvalho Leonardo, Adriana Alberti, France Denoeud, Maria Teresa Barreto Crespo, Jorge Capelo, Frederic Bustos Gaspar
Summary: This study describes the complete chloroplast genome of Centaurium erythraea subsp. majus, the first available plastome belonging to the Centaurium genus. The genome is 153,107 bp long and contains 129 genes, including tRNA, rRNA, and protein-coding genes. Phylogenetic analysis supports the placement of this subspecies under the Gentianaceae family and provides evidence for lineage divergence within the family. This study is important for conservation, phylogenetic and evolutionary studies, and DNA barcoding applications for food, feed, and supplements safety.
MITOCHONDRIAL DNA PART B-RESOURCES
(2023)
Article
Chemistry, Multidisciplinary
Ana Rita Jesus, Alexandre Paiva, Ana Rita C. Duarte
Summary: Natural Deep Eutectic Systems (NADES) have emerged as alternative solvents to traditional organic solvents and ionic liquids, attracting researchers' attention in various fields, particularly in biotechnology. While most research focuses on NADES' use as solubility enhancers in pharmaceutical applications, their applicability in cryopreservation, protein and DNA stabilization, and other biomedical applications is still relatively unexplored. This article discusses current developments and future perspectives in this scientific field.
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Filipa Santos, David Pires, Elsa Anes, Ana Rita C. Duarte
Summary: This study evaluated the stability and antibacterial activity of therapeutic liquid formulations prepared with anti-tuberculosis drugs. The results showed that these mixtures have antibacterial effects against drug-susceptible Mycobacterium tuberculosis strains, with the mixtures incorporating ethambutol showing particularly prominent effects. The findings suggest the potential for further research and evaluation of clinical applicability.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Agriculture, Multidisciplinary
Katarina Cankar, Johanna Christina Hakkert, Robert Sevenier, Christina Papastolopoulou, Bert Schipper, Joao P. . Baixinho, Naiara Fernandez, Melanie S. Matos, Ana Teresa Serra, Claudia Nunes Santos, Khabat Vahabi, Alain Tissier, Paul Bundock, Dirk Bosch
Summary: This study identifies an enzyme in chicory roots that can synthesize a compound with anti-inflammatory activity, and demonstrates that the accumulation of this compound can be increased through gene editing techniques. This contributes to a better understanding of the synthesis pathway of this compound in chicory and highlights the potential for extracting different health products from chicory.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Encarnacion Cruz Sanchez, Maria Teresa Garcia, Joana Pereira, Filipe Oliveira, Rita Craveiro, Alexandre Paiva, Ignacio Gracia, Jesus Manuel Garcia-Vargas, Ana Rita C. Duarte
Summary: This study proposes the use of chitosan (CHT) or alginate (ALG) to prepare membranes for wound healing, with the incorporation of lavender essential oil (LEO). The composition of the membranes was analyzed using Fourier Transform Infrared Spectroscopy (FTIR). The water absorption capacity and oil release profile of the membranes were evaluated, and the cytotoxicity and biocompatibility of the membranes were studied using a HaCaT cell line. The results showed that the CHT/ALG + LEO membrane increased cell proliferation and exhibited potential for wound healing applications.
Article
Chemistry, Multidisciplinary
Sara Valente, Filipe Oliveira, Ines Joao Ferreira, Alexandre Paiva, Rita G. G. Sobral, Mairio S. Diniz, Susana P. P. Gaudencio, Ana Rita Cruz Duarte
Summary: Marine biofouling negatively affects industries with offshore infrastructures and there are currently no ideal solutions to this problem. This study investigates the potential use of hydrophobic deep eutectic systems (HDES) based on menthol and natural organic acids as antifouling coatings. The results show that the HDES Men/OL (1:1) has promising antifouling properties without toxicity to marine species.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Pharmacology & Pharmacy
Celia Sarmento, Hugo Monteiro, Alexandre Paiva, Ana Rita C. Duarte, Ana Rita Jesus
Summary: The goal of this study was to explore the potential of natural deep eutectic systems (NADES) as new media for ocular drug formulations. Different NADES compositions were prepared and characterized for their rheological and physicochemical properties. The results showed that 5-10% (w/v) aqueous solutions of NADES had suitable viscosity, osmolarity, and pH for ocular drug incorporation. By using NADES, the solubility of a highly insoluble drug, acetazolamide, was significantly increased, making it more suitable for ocular drug formulation. Cytotoxicity assays demonstrated that NADES were biocompatible and did not affect cell viability when dissolved in aqueous solutions.
Article
Genetics & Heredity
Ines Carvalho Leonardo, Adriana Alberti, France Denoeud, Maria Teresa Barreto Crespo, Jorge Capelo, Frederic Bustos Gaspar
Summary: This study presents the complete chloroplast genome of Glandora prostrata subsp. lusitanica, the first of its kind for the Glandora genus. The genome is 150,041 bp long with a GC content of 37.5% and displays a quadripartite structure. It contains 131 annotated genes and phylogenetic analysis confirms its placement under the Boraginaceae family. This research will contribute to the conservation, phylogenetic, and evolutionary studies of this traditional species relevant to aromatic, medicinal, and condiment plants from Portugal.
MITOCHONDRIAL DNA PART B-RESOURCES
(2023)
Article
Food Science & Technology
Natasa Nastic, Zorana Mutavski, Jelena Zivkovic, Rita Ambrus, Naiara Fernandez, Nebojsa Menkovic, Senka Vidovic
Summary: The aim of this study was to extract anthocyanin-enriched fractions from black raspberry pomace using ultrasound-assisted extraction and the Particles from Gas-Saturated Solutions process. The results showed that the amplitude of ultrasound had a significant effect on extraction yield and total phenolic content. The high-performance techniques used in this study allowed for the production of natural fractions with desirable characteristics for the development of nutraceuticals and food formulations.
Article
Environmental Sciences
Ines Joao Ferreira, Alexandre Paiva, Mario Diniz, Ana Rita Duarte
Summary: Deep eutectic systems (DES) have gained popularity but their potential toxicity towards living organisms needs further investigation. In this study, the toxicity of two DES and their components were assessed in zebrafish. The results showed no significant toxicity within the tested concentrations, indicating that these DES can be considered as environmentally friendly solvents.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
