Article
Biochemistry & Molecular Biology
Yaxian Wang, Fuchao Zhang, Yunsong Zhang, Qi Shan, Wei Liu, Fengyuan Zhang, Feiyu Zhang, Sheng Yi
Summary: Research findings indicate that Btc plays essential roles in regulating Schwann cell migration and axon elongation, suggesting the potential application of Btc as a regenerative strategy for treating peripheral nerve injury.
MOLECULAR MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Anton Borger, Sarah Stadlmayr, Maximilian Haertinger, Lorenz Semmler, Paul Supper, Flavia Millesi, Christine Radtke
Summary: miRNAs play a crucial role in peripheral nerve injuries by regulating specific pathways and proteins, which is essential for understanding the molecular mechanisms of nerve regeneration and providing targets for precision medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Peng Yu, Guanhua Zhang, Bo Hou, Enpeng Song, Jiaming Wen, Yueyang Ba, Donglin Zhu, Gangwei Wang, Feng Qin
Summary: Complete myelination and formation of myelinated fibers are essential for functional nerve regeneration after peripheral nerve injury. Suboptimal myelin regeneration is common and can hinder ideal nerve regeneration. Therefore, closely monitoring and supporting myelin regeneration in patients with PNI is important for optimal outcomes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Neurosciences
Brendan Zotter, Or Dagan, Jacob Brady, Hasna Baloui, Jayshree Samanta, James L. Salzer
Summary: Peripheral nerves are organized into compartments and Gli1 is a crucial transcriptional effector involved in nerve development, regulation of extracellular matrix production, and nerve vascular organization. Gli1 and Dhh have nonredundant roles in peripheral nerve development.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Neurosciences
Panjian Lu, Gang Wang, Xiaoheng Lu, Pingping Qiao, Yifei Jin, Jun Yu, Qi Chen, Hongkui Wang
Summary: Matrix metalloproteinase 9 (MMP9) is an important gene up-regulated during peripheral nerve regeneration, and it plays a significant role in Schwann cell migration and phenotype modulation. The direct application of MMP9 recombinant protein enhances the regeneration process in injured rats by promoting Schwann cell migration, blood vessel formation, axon elongation, and myelin wrapping. MMP9 regulates both the extracellular and intracellular metabolism of Schwann cells.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Neurosciences
Mar Bosch-Queralt, Robert Fledrich, Ruth M. Stassart
Summary: The Schwann cell (SC), a glial cell in the peripheral nervous system (PNS), is highly versatile in its functions. It plays a crucial role in ensuring the survival of neurons and guiding their axonal development. SCs also contribute to the structural organization of nerves, including blood vessels and various layers. They are involved in myelination of large axons and organization of non-myelinating bundles. SCs have diverse specialized functions in specific locations, such as the neuromuscular junction and cutaneous sensory end organs. They also interact closely with sensory and autonomic neurons. In response to nerve injuries, SCs exhibit remarkable plasticity and transform into repair cells that promote nerve regeneration. However, the molecular mechanisms underlying these functions are still not fully understood.
NEUROBIOLOGY OF DISEASE
(2023)
Article
Neurosciences
Shiying Li, Wenshuang Wu, Jing Zhang, Yu Chen, Yumeng Wu, Xinghui Wang
Summary: The study showed that miR-195-5p is dysregulated after peripheral nerve injury and plays a critical regulatory role in Schwann cell viability, proliferation, and migration. It also affects peripheral nerve regeneration by targeting the Crebl2 gene and modulating Schwann cell functions.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Neurosciences
Haley Jeanette, Leandro N. Marziali, Urja Bhatia, Abigail Hellman, Jacob Herron, Ashley M. Kopec, Maria Laura Feltri, Yannick Poitelon, Sophie Belin
Summary: YAP and TAZ, effectors of the Hippo pathway, play a regulatory role in the proliferation and differentiation of repair Schwann cells during peripheral nerve regeneration, contributing to remyelination over time.
Article
Immunology
Anjali Yadav, Tzu-Chieh Huang, Szu-Han Chen, Thamil Selvee Ramasamy, Yuan-Yu Hsueh, Shau-Ping Lin, Fu- Lu, Ya-Hsin Liu, Chia-Ching Wu
Summary: Inhibiting prolonged inflammation through HDAC inhibitor PBA treatment can suppress the expression and secretion of pro-inflammatory cytokines in SCs, favoring axonal regrowth and remyelination to create a conducive microenvironment for peripheral nerve regeneration.
JOURNAL OF NEUROINFLAMMATION
(2021)
Review
Neurosciences
Qian Wang, Fang-Yu Chen, Zhuo-Min Ling, Wen-Feng Su, Ya-Yu Zhao, Gang Chen, Zhong-Ya Wei
Summary: Peripheral neuropathy is a common neurological issue with limited treatment options. Schwann cell transplantation plays a crucial role in resolving peripheral neuropathy, and stem cells have the potential to differentiate into Schwann-like cells. These studies provide insights into cell therapy and uncover prospective therapeutic strategies for peripheral neuropathy.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Neurosciences
Yunsong Zhang, Yinying Shen, Li Zhao, Qian Zhao, Lili Zhao, Sheng Yi
Summary: Nerve injury-induced Schwann cell dedifferentiation promotes axon growth by creating a favorable microenvironment. Transcription factor BCL11A plays a crucial role in Schwann cell reprogramming during peripheral nerve regeneration. Silencing Bcl11a reduces Schwann cell viability, proliferation, migration rates, and debris clearance ability. Bcl11a deficiency leads to restricted axon elongation and myelin wrapping, resulting in failed recovery. Mechanistically, BCL11A regulates Schwann cell activity by binding to the Nr2f2 promoter and modulating Nr2f2 expression. In conclusion, BCL11A is essential for Schwann cell activation and peripheral nerve regeneration, making it a potential therapeutic target for peripheral nerve injury treatment.
MOLECULAR NEUROBIOLOGY
(2023)
Review
Neurosciences
Qing Min, David B. Parkinson, Xin-Peng Dun
Summary: Schwann cells in the peripheral nervous system have the ability to promote repair of various tissues, including nerve gaps, skin wounds, digit tips, and tooth regeneration. They are highly motile, secrete signaling molecules, attract macrophages, support neuronal survival, promote axonal regrowth, and interact with other cell types in the regeneration process. The migration of Schwann cells is essential for successful tissue repair, especially in cases of peripheral nerve transection injuries where the cells form cords to guide axon regeneration. Understanding the molecular mechanisms of Schwann cell migration may lead to new therapeutic strategies for peripheral nerve repair.
Review
Biotechnology & Applied Microbiology
Jue Ling, Chang He, Shuxuan Zhang, Yahong Zhao, Meifeng Zhu, Xiaoxuan Tang, Qiaoyuan Li, Liming Xu, Yumin Yang
Summary: This article provides an overview of evaluation methods used to study scaffold-based therapies for peripheral nerve injury (PNI) in experimental animal models, with particular focus on Schwann cell functions and axonal growth within the regenerated nerve.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Veterinary Sciences
Tianmei Qian, Pingping Qiao, Yingnan Lu, Hongkui Wang
Summary: The study revealed a potential role of SS18L1 in peripheral nerve injury, showing its influence on nerve recovery process by regulating Schwann cells proliferation, migration, and differentiation.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Neurosciences
Jingmin Liu, Xinrui Ma, Xiaofang Hu, Jinkun Wen, Haowen Zhang, Jiawei Xu, Ye He, Xianghai Wang, Jiasong Guo
Summary: Our study found that RhoA knockdown or inhibition can alleviate the proliferation, migration, and differentiation of Schwann cells. In this study, we developed two lines of conditional RhoA knockout (cKO) mice to investigate the role of RhoA in Schwann cells during nerve injury and repair. Our results indicate that RhoA cKO in Schwann cells can accelerate axonal regrowth and remyelination after sciatic nerve injury, promoting the recovery of nerve conduction and hindlimb gait. Mechanistic studies revealed that RhoA cKO facilitates Schwann cell dedifferentiation via the JNK pathway, subsequently promoting Wallerian degeneration and stimulating the production of neurotrophins.
Article
Neurosciences
Ruirui Zhang, Sailing Chen, Xinghui Wang, Xiaosong Gu, Sheng Yi
Summary: The study used high-coverage single-cell sequencing to reveal the cell diversity and heterogeneity of Schwann cells in the peripheral nerves of neonatal rats. A total of 10 cell types were identified in SN and DRG with distinct cell-cell communication patterns.
Article
Biochemistry & Molecular Biology
Yaxian Wang, Fuchao Zhang, Yunsong Zhang, Qi Shan, Wei Liu, Fengyuan Zhang, Feiyu Zhang, Sheng Yi
Summary: Research findings indicate that Btc plays essential roles in regulating Schwann cell migration and axon elongation, suggesting the potential application of Btc as a regenerative strategy for treating peripheral nerve injury.
MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Qianqian Chen, Qianyan Liu, Yunsong Zhang, Shiying Li, Sheng Yi
Summary: The study reveals the important role of LIF in regulating the proliferation and migration of Schwann cells as well as the regeneration of injured peripheral nerves. Manipulation of LIF expression levels can affect Schwann cell behaviors and impact the process of peripheral nerve regeneration.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Ruirui Zhang, Qi Chen, Li Huang, Yunsong Zhang, Xinghui Wang, Sheng Yi
Summary: The study analyzed single-cell transcriptional profiling of Schwann cells in neonatal rat sciatic nerves, revealing cellular heterogeneity and dynamic changes. It also identified differentiation trajectory from certain Schwann cell subtypes to others, with functional interpretation showing genetic signatures of DNA replication and acquisition of mesenchymal traits in subtype 3 Schwann cells.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Medicine, Research & Experimental
Sailing Chen, Jun Zhu, Yunsong Mang, Xiaodong Cai, Sheng Yi, Xingihui Wang
Summary: This study demonstrated the effects of miR-328a-3p on endothelial cells, showing that transfection with miR-328a-3p mimics decreased HUVEC proliferation while increasing migration and tubulogenesis, with opposite results observed with miR-328a-3p inhibitor. Additionally, potential regulators of miR-328a-3p were identified through bioinformatics analysis and a competing endogenous RNA network centered around miR-328a-3p was constructed.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Neurosciences
Yinying Shen, Zhangchun Cheng, Sailing Chen, Yunsong Zhang, Qi Chen, Sheng Yi
Summary: miR-29a-3p is up-regulated in injured sciatic nerves and inhibits Schwann cell proliferation and migration by negatively regulating PMP22. Injection of miR-29a-3p agomir hinders Schwann cell activities, delays axon elongation and myelination, and retards the functional recovery of injured nerves. This highlights the important role of miR-29a-3p/PMP22 in regulating Schwann cell phenotype following sciatic nerve injury and peripheral nerve regeneration.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Cell Biology
Yin-Ying Shen, Rui-Rui Zhang, Qian-Yan Liu, Shi-Ying Li, Sheng Yi
Summary: Cellular senescence and proliferation are essential for wound healing and tissue remodeling. This study analyzed gene expression patterns in rat sciatic nerve stumps and dorsal root ganglia after injury to reveal the genetic changes associated with cellular senescence and proliferation. The study found that cellular senescence and proliferation were less vigorous in the dorsal root ganglia compared to the sciatic nerve stumps.
NEURAL REGENERATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yunsong Zhang, Miao Yang, Yinying Shen, Sheng Yi, Xinghui Wang
Summary: This study found that MOGS is upregulated in nerve segments after peripheral nerve injury, and bioinformatic analysis indicated its involvement in cellular metabolism and negative regulation on Schwann cell plasticity and metabolic coupling with axons.
JOURNAL OF MOLECULAR NEUROSCIENCE
(2022)
Article
Cell & Tissue Engineering
Xiaokun Gu, Sheng Yi, Aidong Deng, Hong Liu, Lai Xu, Jianhui Gu, Xiaosong Gu
Summary: Chitosan-PGLA nerve grafts combined with bone marrow mononuclear cells (BMMCs) can effectively repair long-distance peripheral nerve defects in the upper arm and achieve good clinical results. Sensory and motor functions gradually restored after surgery, allowing the patient to perform daily activities, and there was a significant increase in skin perfusion.
CURRENT STEM CELL RESEARCH & THERAPY
(2022)
Article
Neurosciences
Yunsong Zhang, Qian Zhao, Qianqian Chen, Lingchi Xu, Sheng Yi
Summary: Transcription factors play important roles in nerve regeneration after peripheral nerve injury, including regulating axon elongation and Schwann cell phenotype modulation. Understanding these transcription factors can help promote functional recovery of injured peripheral nerves.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Cell Biology
Qian-Qian Chen, Qian-Yan Liu, Pan Wang, Tian-Mei Qian, Xing-Hui Wang, Sheng Yi, Shi-Ying Li
Summary: Neurotrophic factors, especially nerve growth factor, can enhance neuronal regeneration, but their in vivo applications are limited. This study investigated the potential of let-7 as a regulator in nerve repair, as it targets and regulates nerve growth factor. Anti-let-7a was identified as the most suitable let-7 family molecule, and its controlled delivery was achieved using a chitosan-hydrogel scaffold, promoting nerve regeneration and functional recovery in a rat model of sciatic nerve transection.
NEURAL REGENERATION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Qianqian Chen, Yang Miao, Zhipeng Xu, Ruirui Zhang, Sheng Yi
Summary: Schwann cells play a crucial role in nerve regeneration and are commonly used in tissue engineering. Identifying Schwann cells with enhanced migration ability is important for improving recovery effects. This study compared Schwann cells from different anatomical locations and found that dorsal root ganglia (DRG) Schwann cells have faster migration speed than sciatic nerve (SN) Schwann cells.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Neurosciences
Yunsong Zhang, Yinying Shen, Li Zhao, Qian Zhao, Lili Zhao, Sheng Yi
Summary: Nerve injury-induced Schwann cell dedifferentiation promotes axon growth by creating a favorable microenvironment. Transcription factor BCL11A plays a crucial role in Schwann cell reprogramming during peripheral nerve regeneration. Silencing Bcl11a reduces Schwann cell viability, proliferation, migration rates, and debris clearance ability. Bcl11a deficiency leads to restricted axon elongation and myelin wrapping, resulting in failed recovery. Mechanistically, BCL11A regulates Schwann cell activity by binding to the Nr2f2 promoter and modulating Nr2f2 expression. In conclusion, BCL11A is essential for Schwann cell activation and peripheral nerve regeneration, making it a potential therapeutic target for peripheral nerve injury treatment.
MOLECULAR NEUROBIOLOGY
(2023)
Review
Neurosciences
Lili Zhao, Weixiao Huang, Sheng Yi
Summary: Single-cell RNA sequencing enables the classification and transcriptional profiling of individual cells, leading to a better understanding of cellular diversity. In the peripheral nervous system (PNS), this technique has identified multiple cell types including neurons, glial cells, ependymal cells, immune cells, and vascular cells. Subtypes of neurons and glial cells have also been recognized in different physiological and pathological states. This article summarizes the heterogeneity of cells in the PNS and describes cellular variability during development and regeneration, which provides insights into the complex cellular architecture of the PNS and serves as a basis for future genetic manipulation.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Sailing Chen, Qianqian Chen, Xiaojiao Zhang, Yinying Shen, Xinyu Shi, Xiu Dai, Sheng Yi
Summary: Peripheral nerves have limited ability to regenerate after injury. The growth factor amphiregulin (AREG) is upregulated in Schwann cells of injured sciatic nerves and stimulates their proliferation and migration. Schwann cell-secreted AREG promotes neurite outgrowth and axon elongation. Administering AREG to injured nerves facilitates Schwann cell proliferation, cord formation, and axon regrowth. Overall, our study identifies AREG as an important neurotrophic factor and offers a promising therapeutic approach for peripheral nerve injuries.
JOURNAL OF NEUROCHEMISTRY
(2023)