Article
Chemistry, Applied
Christopher David Viljoen, Chantelle Booysen, Sandhya Sreenisvasan Tantuan
Summary: Detecting species using DNA extracted from processed foods is important for food authenticity testing. It can be challenging to extract DNA from processed foods, and determining the concentration and purity of extracted DNA using spectrophotometry is considered essential. However, the impact of chemical residue from the processed food matrix on spectrophotometry readings has not been investigated.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Engineering, Chemical
Yingwei Lv, Yubo Liu, Baozhong Ma, Chengyan Wang, Yongqiang Chen
Summary: This study focuses on the recovery of rubidium from high concentration impurity solution through solvent extraction, and further purification and refinement of rubidium salt products. The results show that rubidium can be completely extracted after four-stage countercurrent extraction, accompanied by 18.84% of potassium entering the organic phase. By further scrubbing and HCl stripping, high-purity rubidium chloride and rubidium carbonate products can be obtained.
Article
Biochemical Research Methods
Shuaishuai Li, Xiaolei Liu, Ziye Li, Hong Liu, Dawei Hu
Summary: Extraction of DNA is crucial for molecular biology experiments and counting microbial individuals. Direct boiling and mechanical lysis with glass beads are two physical extraction methods that overcome limitations of popular reagent kits or traditional spread plate method during non-equilibrium phenomenon. Combining these methods resulted in increased DNA purity and qPCR results closer to direct microscopy counting, providing a new choice for extracting bacterial DNA in specific circumstances.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Chemistry, Analytical
Susanne Pahlow, Stefanie Hentschel, Peter Horbert, Cynthia Romero, Lydia Lehniger, Sascha Wagner, Jurgen Popp, Karina Weber
Summary: Introduction of a 3D-printed cartridge system for convenient and cost-efficient sample preparation from sputum for subsequent PCR detection. The system operates on pneumatic actuations with a closed design to minimize contamination risk. Enrichment of bacterial cells using amine-functionalized particles allows for intact and viable cells, suitable for various detection methods including PCR.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Applied
Pierre Degot, Verena Huber, Didier Touraud, Werner Kunz
Summary: Curcumin was extracted from Curcuma Longa using a surfactant-free microemulsion, producing highly concentrated tinctures of up to 130 mg/mL curcuminoids. Water content influenced the extraction process and the purity of the extracts, with additional purification steps enhancing purity to 94%. Purification before extraction allowed for indefinite solubilization of water insoluble curcumin extracts in aqueous environments, with stability of the solutions for up to five months when kept away from natural light.
Article
Plant Sciences
Jinwoo Jang, Haewon Jeong, Eunjae Jang, Eungpil Kim, Youngdae Yoon, Sujeong Jang, Han-Seong Jeong, Geupil Jang
Summary: This study compared three methods for extracting exosomes from ginseng, and found that the combination of ultracentrifugation and ExoQuick methods can obtain exosomes with high purity and stability. It was also demonstrated that this method is suitable for isolating high-purity and high-stability exosomes from the model plant Arabidopsis.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Liang Ying Ee, Yong Kuok Tan, Jiapei Miao, Hui Ting Chu, Sam Fong Yau Li
Summary: Reusable acidic ternary deep eutectic solvents (TDES) composed of alanine, lactic acid, and ethylene glycol were designed for the fractionation of high-purity lignin and augmentation of lignocellulosic biomass. The TDES extraction method yielded lignin free of sulfur and silica, with desirable molecular weight and monomeric yield, which can be beneficial for various applications. This green extraction method provides a new approach for sustainable biomass valorization.
Article
Biotechnology & Applied Microbiology
Mathieu Gand, Bram Bloemen, Kevin Vanneste, Nancy H. C. Roosens, Sigrid C. J. De Keersmaecker
Summary: In this study, the performances of 6 DNA extraction methods were evaluated for long-read sequencing. The Quick-DNA HMW MagBead Kit (Zymo Research) was found to produce the highest yield of pure high molecular weight DNA and is recommended for bacterial metagenomics studies using Nanopore sequencing.
Article
Chemistry, Analytical
Anand Baby Alias, Hong-Yuan Huang, Yi-Wen Wang, Kai-Ti Lin, Pei-Jhen Lu, Tzu-Hui Wu, Pei-Shin Jiang, Chien-An Chen, Da-Jeng Yao
Summary: This study utilizes Electro-Wetting-On-Dielectric (EWOD) based digital operations to extract subcritical concentration of cell-free DNA (cf-DNA), and amplifies it to the nanogram range for further analysis. The natural growth of the embryo is not disrupted during the extraction process, paving a new path for the lab-on-a-chip (LOC) concept.
Article
Multidisciplinary Sciences
Hyeon-Ki Jang, Dong Hyun Jo, Seu-Na Lee, Chang Sik Cho, You Kyeong Jeong, Youngri Jung, Jihyeon Yu, Jeong Hun Kim, Jae-Sung Woo, Sangsu Bae
Summary: Ribonucleoprotein (RNP) complex-mediated base editing is promising for therapeutic applications due to its reduced off-target effects. However, obtaining highly purified CBE/ABE proteins from bacterial systems is challenging. ABE RNPs showed significantly reduced off-target effects in both DNA and RNA compared to plasmid-encoded ABE.
Article
Engineering, Chemical
Fan Yu, Yulu Du, Shaopo Deng, Mingjie Jin, Deyu Zhang, Mengqian Zhao, Jian Yin, Xuwei Long
Summary: In this study, a newly efficient extraction process using ethanol was developed for obtaining high-purity surfactin from fermentation broth. The use of ethanol as a solvent provided a high purity of 98% and recovery of over 90%, and the surfactin isolated using ethanol extraction contained fewer impurities compared to dichloromethane extraction. This environmentally-friendly extraction process has excellent separation performance and is suitable for isolating surfactin for high-value applications.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Qiang Yu, Xia Hua, Xin Zhou, Bin Xu, Huan Wang, Yong Xu
Summary: In this study, a novel and environmentally friendly bioconversion technology was proposed to prepare high-purity glycolic acid (GA) efficiently. By integrating resting cell catalysis, crystallization, acidification, and electrodialysis purification, continuous and efficient GA bio-preparation was achieved.
Article
Chemistry, Physical
Laurent Lermusiaux, Anum Nisar, Alison M. Funston
Summary: This study introduces a simple and versatile method to assemble nanoparticles of different sizes, shapes, and materials using only two complementary DNA strands, forming high-purity homo- or hetero-structures with various geometries, which can be applied in biological and optical fields.
Review
Chemistry, Multidisciplinary
Jingyi Wang, Ting Lei
Summary: This article discusses the separation methods for enriching large-diameter s-SWCNTs and their advantages in electronic applications, including CPW, GC, ATPE, and DGU technologies. By separating based on the electronic type and chirality of SWCNTs, important references can be provided for the future development of SWCNT-based electronic devices.
Article
Engineering, Chemical
Sahand Fakurpur Shirejini, Seyed Mohsen Dehnavi, Mehdi Jahanfar
Summary: The study investigates the potential of iron oxide nanoparticles coated with carbon dots (SPION@CDs) as a magnetic nano-adsorbent for DNA bioseparation. Core-shell SPION@CDs with a pH-sensitive property were synthesized using a one-step solvothermal method. The nanohybrid exhibited efficient DNA isolation and release at different pH values, with a maximum adsorption capacity of 125.12 μg/mg. It demonstrated high biocompatibility and low toxicity, making it a promising candidate for biological applications.
CHEMICAL ENGINEERING RESEARCH & DESIGN
(2023)
