Review
Biochemistry & Molecular Biology
Elancheleyen Mahindran, Wan Safwani Wan Kamarul Zaman, Khairul Bariah Ahmad Amin Noordin, Yuen-Fen Tan, Fazlina Nordin
Summary: Population ageing is increasing globally due to advancements in medical treatments and technologies. This leads to a higher prevalence of age-related diseases, such as muscle loss, among the older population. Current interventions only slow down and control symptoms, and there is no treatment to prevent or reverse age-related muscle frailty. Mesenchymal stem cell-derived extracellular vesicle (MSC-EV) therapy shows promise in attenuating age-related muscle frailty, but further studies, including large-scale clinical trials, are needed to determine its effectiveness.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell & Tissue Engineering
Yuling Huang, Lina Yang
Summary: This review summarizes the preclinical research status of MSC-EVs in treating fibrotic diseases, highlighting their potential superiority over MSCs. However, there is still a significant knowledge gap in treating fibrosis of other tissues and organs, which requires further exploration.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Medicine, Research & Experimental
Xiaoying Wang, Lili Jiang, Qifang Liu
Summary: This study demonstrated that human mesenchymal stem cell-derived extracellular vesicles (hMSC-EVs) inhibit ovarian cancer cell growth, migration, invasion, and chemotherapy resistance by carrying miR-18a-5p. miR-18a-5p targets NACC1, and the activation of the AKT/mTOR pathway is involved in this process.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Wanrong Meng, Linlin Wang, Xueyu Du, Mingzhe Xie, Fan Yang, Fei Li, Zhanxuan E. Wu, Jianguo Gan, Hongxuan Wei, Chang Cao, Shun Lu, Bangrong Cao, Longjiang Li, Ling Li, Guiquan Zhu
Summary: Extracellular vesicles (EVs) are potential therapeutic carriers, but they have limited targeting ability. To address this issue, researchers genetically engineered natural free streptavidin (SA) on the cellular surface of bone marrow mesenchymal stem cells (BMSCs) to obtain BMSC-derived EVs (BMSC-EVs) with enhanced targetability. The study demonstrated that these BMSC-EVs could be modified with different molecules to achieve specific delivery of drugs to different types of tumors, making them a versatile platform for targeted drug delivery.
JOURNAL OF CONTROLLED RELEASE
(2023)
Review
Cell & Tissue Engineering
Davod Pashoutan Sarvar, Hosein Effatpanah, Parvin Akbarzadehlaleh, Karim Shamsasenjan
Summary: The study highlights the important role of MSC-derived extracellular vesicles (MSC-EVs) in hematopoietic regulation and immune response after transplantation. MSC-EVs directly fuse with hematopoietic stem cell membranes, affecting their fate, and regulate the proliferation and differentiation of hematopoietic stem cells through the release of various bioactive factors.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Immunology
Min Jiang, Xupin Jiang, Hongmei Li, Can Zhang, Ze Zhang, Chao Wu, Junhui Zhang, Jiongyu Hu, Jiaping Zhang
Summary: Diabetic foot is a common complication of diabetes and often leads to amputation. Previous studies have shown that stem cell transplantation can promote the healing of chronic diabetic wounds, but safety concerns have limited its clinical application. Recently, it has been discovered that mesenchymal stem cell-derived extracellular vesicles (EVs) have similar therapeutic effects and offer advantages such as safety and reduced immune response. This paper summarizes the current understanding of mesenchymal stem cell-derived EVs in accelerating the healing of diabetic wounds and their potential clinical applications.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Akaitz Dorronsoro, Fernando E. Santiago, Diego Grassi, Tianpeng Zhang, Ruenn Chai Lai, Sara J. McGowan, Luise Angelini, Mitra Lavasani, Lana Corbo, Aiping Lu, Robert W. Brooks, Marta Garcia-Contreras, Donna B. Stolz, Antonio Amelio, Siddaraju V. Boregowda, Mohammad Fallahi, Adrian Reich, Camillo Ricordi, Donald G. Phinney, Johnny Huard, Sai Kiang Lim, Laura J. Niedernhofer, Paul D. Robbins
Summary: The injection of BM-MSCs from young mice can prolong lifespan and healthspan, while conditioned media (CM) from young BM-MSCs can rescue the function of aged stem cells and senescent fibroblasts. Extracellular vesicles (EVs) from young BM-MSC CM can extend lifespan in mice, and EVs from MSCs generated from human ES cells can reduce senescence and improve healthspan.
Review
Cell Biology
Huixue Tang, Huikun Luo, Zihan Zhang, Di Yang
Summary: Mesenchymal stem cells (MSCs) are a type of cells with multipotency and self-renewal ability, and their secreted apoptotic bodies (ABs) have important biological functions and great therapeutic potential.
Review
Cell & Tissue Engineering
Ghaidaa Raheem Lateef Al-Awsi, Fahad Alsaikhan, Ria Margiana, Irfan Ahmad, Indrajit Patra, Mazin A. A. Najm, Ghulam Yasin, Iroda Rasulova, Ali Thaeer Hammid, Hamzah H. Kzar, Moaed E. Al-Gazally, Homayoon Siahmansouri
Summary: Breast cancer is the second most frequently diagnosed cancer and the leading cause of cancer death in women. Mesenchymal stem cells (MSCs) are a subgroup of cells that can differentiate into multiple cell types and have been found to have various effects on tumor growth and development. Recent studies have shown that MSCs can both promote and inhibit tumor development. Exosomes (Exo) have emerged as a new mechanism of cell communication, and MSC-derived exosomes (MSCs-Exo) have been found to carry molecules that can affect the proliferation, metastasis, and drug response of breast cancer cells.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Chemistry, Multidisciplinary
Zeyuan Cao, Peiyi Li, Yuzhen Li, Manjin Zhang, Meng Hao, Wenwen Li, Xueli Mao, Lijie Mo, Chuan Yang, Xin Ding, Yi Yan Yang, Peiyan Yuan, Songtao Shi, Xiaoxing Kou
Summary: Extracellular vesicles (EVs) are lipid bilayer nanovesicles that can transport various substances and play important roles in cell communication and tissue homeostasis. In this study, it is shown that apoptotic mesenchymal stem cells (MSCs) can efficiently encapsulate nanoparticles into apoptotic vesicles (apoVs). The researchers also demonstrated that nano-bortezomib-apoVs have a synergistic effect on improving multiple myeloma (MM) and reducing side effects. Furthermore, they discovered that Rab7 regulates the encapsulation efficiency of nanoparticles in apoptotic MSCs.
Article
Medicine, Research & Experimental
Allaura S. Cone, Xuegang Yuan, Li Sun, Leanne C. Duke, Michael P. Vreones, Allison N. Carrier, Stephanie M. Kenyon, Spencer R. Carver, Sarah D. Benthem, Alina C. Stimmell, Shawn C. Moseley, David Hike, Samuel C. Grant, Aaron A. Wilber, James M. Olcese, David G. Meckes
Summary: Research has shown that extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) have potential therapeutic effects for Alzheimer's disease, slowing down disease progression and improving cognitive function when administered intranasally in mice.
Article
Multidisciplinary Sciences
Maoxiao Ma, Guofeng Cui, Youwen Liu, Yanfeng Tang, Xiaoshuai Lu, Chen Yue, Xue Zhang
Summary: Mesenchymal stem cells (MSCs) exert their therapeutic effects through the secretion of extracellular vesicles (EVs), which can transport various molecules and modify the development of diverse diseases. MSC-derived EVs participate in bone regeneration and the pathophysiology of orthopedic diseases by regulating inflammation, promoting angiogenesis, and influencing the differentiation and proliferation of osteoblasts and osteoclasts. This review summarizes the recent advancements in the application and functions of MSC-derived EVs as potential therapies against orthopedic diseases.
Review
Biotechnology & Applied Microbiology
Yuanliang Xia, Ruohan Yang, Yulin Hou, Hengyi Wang, Yuehong Li, Jianshu Zhu, Changfeng Fu
Summary: Intervertebral disc degeneration is a major cause of lower back pain, and current treatments have limitations. Cell therapy using extracellular vesicles shows potential for treating this condition.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Cell & Tissue Engineering
Ahmed Lotfy, Noha M. AboQuella, Hongjun Wang
Summary: Mesenchymal stromal/stem cells (MSCs) are widely used in cell therapy due to their immunomodulatory and regenerative properties. The production of various factors via extracellular vesicles, especially exosomes, governs the efficacy of MSCs. MSC-derived exosomes (MSC-Exos) show advantages over MSCs themselves by reducing adverse effects. Clinical studies are assessing the therapeutic effect of MSC-Exos in different diseases, and production methods, dosage, and administration routes are being explored.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Engineering, Biomedical
Calvin Chang, Jerry Yan, Zhicheng Yao, Chi Zhang, Xiaowei Li, Hai-Quan Mao
Summary: This review focuses on the bioactivity and secretome of mesenchymal stem cells, as well as the engineering approaches to tune the MSC secretome. It also discusses the recent advances in biomaterials-based therapeutic strategies for delivering MSCs and MSC-derived secretome in extracellular vesicles, along with their advantages and challenges.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Juan A. Fafian-Labora, Miriam Morente-Lopez, Fco Javier de Toro, Maria C. Arufe
Summary: HGPS is a deadly childhood disorder caused by a mutation on the LMNA gene, characterized by accelerated aging. Research has shown alterations in the Ca2+ signaling pathway in HGPS, possibly due to the overproduction of ROS. This opens up new therapeutic strategies for this rare disease and provides insights into pathologies involving accelerated and healthy aging.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Surgery
Alba Gonzalez Rodriguez, Sara A. Gonzalez Porto, Nerea Comellas Melero, Maria C. Arufe
Summary: This study investigated the effects of BM-MSCs associated with ANAs on phrenic nerve transfer in a rat model of upper trunk avulsion. The results showed significant improvements in latency and amplitude of the compound motor action potential in the ANAs + BM-MSCs group, indicating a positive effect on nerve regeneration.
Article
Cell Biology
Paula Carpintero-Fernandez, Michela Borghesan, Olga Eleftheriadou, Belen Pan-Castillo, Juan Antonio Fafian-Labora, Tom P. Mitchell, Alejandro Yuste, Muge Ogrunc, Thomas D. Nightingale, Maria Mayan, Ana O'Loghlen
Summary: In this study, a functional genome-wide CRISPR/Cas9 genetic screen was used to identify key genes involved in the proliferation arrest induced by CDK4/6 inhibitors. Downregulation of coagulation factor IX (F9) was found to prevent cell cycle arrest and senescent-like phenotype in breast tumor cells treated with Palbociclib. These findings can contribute to the design of new therapeutic strategies in personalized medicine to increase treatment efficiency, stratify patients, and overcome drug resistance.
CELL DEATH & DISEASE
(2022)
Review
Biology
Rocio Mato-Basalo, Sergio Lucio-Gallego, Carmen Alarcon-Veleiro, Marta Sacristan-Santos, Maria del Pilar Miranda Quintana, Miriam Morente-Lopez, Francisco Javier de Toro, Lucia Silva-Fernandez, Alba Gonzalez-Rodriguez, Maria C. Arufe, Juan Antonio Fafian Labora
Summary: Inflammaging, the accumulation of proinflammatory components due to aging, affects intercellular communication. Recent studies have highlighted the role of small extracellular vesicles (sEVs) as mediators in paracrine senescence and inflammatory aging. Understanding the components and mechanisms of action of sEVs is crucial for the development of senodrugs that regulate intercellular communication in inflammaging and its related diseases such as cancer and type 2 diabetes.
Article
Biochemistry & Molecular Biology
Miriam Morente-Lopez, Rocio Mato-Basalo, Sergio Lucio-Gallego, Lucia Silva-Fernandez, Alba Gonzalez-Rodriguez, Fco Javier De Toro, Juan A. Fafian-Labora, Maria C. Arufe
Summary: This study found that extracellular vesicles (EV) derived from mesenchymal stem cells (MSC) modified to inhibit the expression of miR-21 have a higher therapeutic potential in treating osteoarthritis (OA). These EV effectively reduced inflammation in an OA animal model compared to MSC alone, and their therapeutic effect was validated through proteomic and genomic techniques.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Carmen Alarcon-Veleiro, Rocio Mato-Basalo, Sergio Lucio-Gallego, Andrea Vidal-Pampin, Maria Quindos-Varela, Thamer Al-Qatarneh, German Berrecoso, Angel Vizoso-Vazquez, Maria C. Arufe, Juan Fafian-Labora
Summary: Epithelial ovarian cancer (EOC) is the most lethal gynecological cancer, and current treatment methods are not satisfactory due to the development of chemo-resistance. Recently, ferroptosis, a new form of regulated cell death, has been found to be a promising anti-tumor strategy for EOC treatment.
Meeting Abstract
Biotechnology & Applied Microbiology
M. Morente-Lopez, R. Mato-Basalo, S. Lucio-Gallego, L. Silva, A. Garcia-Sanchez, F. J. De Toro, J. A. Fafian-Labora, M. Arufe
HUMAN GENE THERAPY
(2022)
Meeting Abstract
Biotechnology & Applied Microbiology
D. Miranda-Balbuena, J. Lopez-Seijas, M. Sacristan-Santos, N. Carballo-Pedrares, M. C. Arufe, J. Fafian-Labora, A. Rey-Rico
HUMAN GENE THERAPY
(2022)
Meeting Abstract
Biotechnology & Applied Microbiology
M. Morente-Lopez, R. Mato-Basalo, P. Miranda, J. Fafian-Labora, C. Gil, M. Carrera, M. C. Arufe
HUMAN GENE THERAPY
(2021)