Article
Cell Biology
Rebecca A. Rolfe, Claire A. Shea, Paula Murphy
Summary: The spatial and temporal control of chondrogenesis is crucial for generating precise skeletal structures in the developing vertebrate limb. The interplay between BMP and Wnt signaling pathways plays a fundamental role in organizing complex patterns. Through the micromass culture model, this study investigates the geometric properties and spatial relationships between chondrogenic condensations, and explores the influence of BMP and Wnt ligands on differentiation profile and nodule pattern. The results support the hypothesis that the balance of Wnt and BMP signaling under a Turing-like mechanism influences the formation of regularly spaced cartilage nodules.
CELL AND TISSUE RESEARCH
(2022)
Article
Materials Science, Biomaterials
Ramesh Subbiah, Gabriela de Souza Balbinot, Avathamsa Athirasala, Fabricio Mezzomo Collares, Grigoriy Sereda, Luiz E. Bertassoni
Summary: Traditional biomaterials for bone regeneration lack the ability to fully replicate the native bone microenvironment, but a novel approach involving biomimetic mineralization using GelMA hydrogels and calcium citrate core-shell microparticles shows promising results in promoting osteogenic differentiation for bone regeneration. This method successfully achieves biomineralization of the scaffolds, resembling the mineral chemistry, structure, and organization of native bone, ultimately enhancing the potential for bone regeneration efforts.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Cell Biology
Judit Vago, Katalin Kiss, Edina Karanyicz, Roland Takacs, Csaba Matta, Laszlo Ducza, Tibor A. Rauch, Roza Zakany
Summary: The study demonstrated the significant role of DNA demethylation enzyme Tet1 in chondrogenesis, and found that inhibition of DNA methylation had distinct effects on different stages of in vitro cartilage formation.
Article
Engineering, Biomedical
Huimin Shi, Yang Li, Kailei Xu, Jun Yin
Summary: The development of a methacrylated collagen-based bioink with good bioactivity and printability is described in this study. The bioink showed similar printability to methacrylated gelatin in extrusion bioprinting and better formability in digital light processing. Cell-laden bioinks using this CMA bioink demonstrated improved cell proliferation, cell spreading, and gene expression compared to gelatin-based bioinks.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Biomedical
Qingli Liu, Wenling Dai, Yongli Gao, Longpeng Dong, Hengxing Jia, Shikui Li, Likun Guo, Yujiang Fan, Xingdong Zhang
Summary: The physicochemical properties of collagen-based hydrogels and co-culture system have synergistic effects on cartilage formation. The spreading and proliferation of cells are regulated by the degradation rate and stiffness of hydrogels. Co-culture cells in collagen-based hydrogels significantly improve chondrogenesis.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Multidisciplinary
Sara Borrego-Gonzalez, Matthew J. Dalby, Aranzazu Diaz-Cuenca
Summary: The study achieved improved biomimicry in a nanofibrous gelatin matrix through the incorporation of a minimal content of D-periodic self-assembled atelocollagen. The results demonstrated a significant increase in cell attachment and proliferation by introducing 2.6 wt.% D-periodic atelocollagen into the gelatin material, compared to bare gelatin matrices, indicating a cost-effective and low immunogenic collagen-like biomaterial route based on gelatin.
Article
Materials Science, Biomaterials
Nele Pien, Daniele Pezzoli, Jasper Van Hoorick, Francesco Copes, Margot Vansteenland, Madalina Albu, Bruno De Meulenaer, Diego Mantovani, Sandra Van Vlierberghe, Peter Dubruel
Summary: This study developed COL-MA and GEL-MA, collagen-based hydrogel precursors functionalized for photo-crosslinking, providing a new approach for vascular tissue engineering applications. The derivatives showed high crosslinking efficiency, tunable mechanical properties, and superior cell interactions compared to GEL-MA.
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Cell Biology
Wen Wang, Shengnan Qin, Peiliang He, Wei Mao, Liang Chen, Xing Hua, Jinli Zhang, Xifeng Xiong, Zhihe Liu, Pengzhen Wang, Qingqi Meng, Fei Dong, Aiguo Li, Honghui Chen, Jiake Xu
Summary: The study showed that CII-sponges facilitated the differentiation of TSPCs into chondrocytes and increased the area of fibrocartilage transition zones, suggesting their significance in FC reconstruction at bone-tendon junction. Further research and validation are needed to establish the relationship between these two phenomena.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Yumi Miyasaka, Nobuhiko Yamamoto
Summary: During development, physiological neuronal activity modulates the expression of wiring molecules by altering gene expression in cortical circuits. The study showed that patterned stimuli regulated BDNF promoter activity differently in upper layer neurons, with increases in promoter activity roughly proportional to the increase in intracellular Ca2+ concentration per unit time. These results suggest that physiological stimulation patterns tune activity-dependent gene expression in developing cortical neurons via cortical circuits and altering intracellular calcium signaling.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Orthopedics
M. Abdulhadi Alagha, Judit Vago, Eva Katona, Roland Takacs, Daan Van der Veen, Roza Zakany, Csaba Matta
Summary: This study found that the core circadian clock genes exhibit rhythmic expression patterns during early chondrocyte differentiation, leading to synchronized expression of chondrogenic transcription factors and developing cartilage matrix constituents. This synchronized clock function positively influences tissue homeostasis and histogenesis during early chondrogenesis.
Article
Engineering, Biomedical
Boudewijn van der Sanden, Laetitia Gredy, Didier Wion, Olivier Stephan
Summary: The study used two-photon polymerization to create GelMA-collagen matrixes for monitoring pO(2matrix) in 3D cell cultures. The fluorescence intensity of ruthenium complexes showed a direct and inverse relationship with local pO(2matrix), with a significant increase observed during cancer cell activities. This approach has the potential for studying cancer cell metabolic adaptations and developing new therapeutic opportunities.
ACTA BIOMATERIALIA
(2021)
Article
Materials Science, Biomaterials
John Redmond, Helen O. McCarthy, Paul Buchanan, Tanya J. Levingstone, Nicholas J. Dunne
Summary: 2D culture lacks proper cell-cell and cell-matrix interactions, resulting in exaggerated responses to therapeutic agents. 3D models, such as the collagen-based scaffold developed in this study, offer a more accurate representation of in vivo cancers and have the potential to explore gene expression profiles and assess the efficacy of therapeutic agents in treating breast cancer.
BIOMATERIALS ADVANCES
(2022)
Article
Multidisciplinary Sciences
Chutirat Torsahakul, Nipan Israsena, Supaporn Khramchantuk, Juthamas Ratanavaraporn, Sirakarnt Dhitavat, Watchareewan Rodprasert, Sirirat Nantavisai, Chenphop Sawangmake
Summary: This study aimed to generate tissue-engineered canine cornea using canine limbal epithelial stem cells (cLESCs) and canine corneal stromal stem cells (cCSSCs). The results showed that using silk fibroin/gelatin as a substrate promoted cell adhesion, viability, and proliferation, and differentiated keratocytes expressed proteins associated with native cornea.
Article
Cell Biology
Atefeh Atefi, Pendar Shojaei Kojouri, Fereshteh Karamali, Shiva Irani, Mohammad Hossein Nasr-Esfahani
Summary: This study investigates the regulatory regions of the human RAX gene and validates the potential promoter activity in cells. The findings offer insights into the vital functions of the RAX gene in retina development.
BMC MOLECULAR AND CELL BIOLOGY
(2021)
Article
Orthopedics
Julien Guerrero, Sonja Hackel, Andreas S. Croft, Christoph E. Albers, Benjamin Gantenbein
Summary: Through studying the influence of culture methods and microenvironment on human nucleus pulposus progenitor cells, it was found that expansion in a three-dimensional environment better preserved Tie2(+) cell populations and increased chondrogenic and osteogenic differentiation potential. Maintaining pluripotent gene expression was mainly attributed to the higher presence of Tie2(+) cells in the three-dimensional culture.