Article
Biochemistry & Molecular Biology
Tiziana Filardi, Giuseppina Catanzaro, Giuseppina Emanuela Grieco, Elena Splendiani, Sofia Trocchianesi, Carmela Santangelo, Roberto Brunelli, Elisa Guarino, Guido Sebastiani, Francesco Dotta, Susanna Morano, Elisabetta Ferretti
Summary: This study found that miR-222-3p and miR-409-3p are differentially expressed in gestational diabetes mellitus (GDM) and are correlated with fasting plasma glucose, birth weight, and gestational age. The deregulated miRNAs may be associated with the pathophysiology of type 2 diabetes and GDM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Obstetrics & Gynecology
M. Insenser, A. Quintero, S. de Lope, F. Alvarez-Blasco, M. A. Martinez-Garcia, M. Luque-Ramirez, H. F. Escobar-Morreale
Summary: This research found that the expression of miR-142-3p and miR-598-3p, which are associated with polycystic ovary syndrome (PCOS), differs significantly between women with PCOS and those without. These circulating miRNAs, when combined with easily available measurements such as waist-to-hip ratio (WHR) and circulating LH-to-FSH (LH/FSH) ratios, can effectively distinguish between women with and without PCOS.
HUMAN REPRODUCTION
(2023)
Article
Nutrition & Dietetics
Kirsti Krohn Garnaes, Trude Elvebakk, Oyvind Salvesen, Signe Nilssen Stafne, Siv Morkved, Kjell asmund Salvesen, Trine Moholdt
Summary: Women with obesity are at increased risk for hyperglycemia during pregnancy. A study found associations between early pregnancy dietary intake and late pregnancy glycemia in women with obesity. However, there were no clear associations between single dietary variables and gestational diabetes mellitus diagnosis.
Article
Medical Laboratory Technology
Mina Tutunfroush, Saeid Ghorbian, Jafar Mohseni, Shahla Danaii, Mehdi G. Rad
Summary: This study quantifies miR-23a-3p, miR-101-3p, and miR-let-7c expression levels in plasma samples of patients with idiopathic recurrent pregnancy loss (iRPL) and evaluates their potential diagnostic value. Downregulation of these microRNAs is associated with iRPL and the plasma levels are negatively correlated with the number of abortions.
CLINICAL LABORATORY
(2022)
Article
Biochemistry & Molecular Biology
Michele Manganelli, Ilaria Grossi, Manuela Ferracin, Paola Guerriero, Massimo Negrini, Michele Ghidini, Chiara Senti, Margherita Ratti, Claudio Pizzo, Rodolfo Passalacqua, Sarah Molfino, Gianluca Baiocchi, Nazario Portolani, Eleonora Marchina, Giuseppina De Petro, Alessandro Salvi
Summary: The study investigated the longitudinal levels of cell-free long ncRNA GAS5 and microRNAs miR-126-3p and miR-23b-3p in patients with hepatocellular carcinoma (HCC) undergoing sorafenib treatment. The results highlighted the significant downregulation of miR-23b-3p in untreated HCC patients and its diagnostic relevance.
Article
Nutrition & Dietetics
Aikaterini Apostolopoulou, Antigoni Tranidou, Violeta Chroni, Ioannis Tsakiridis, Emmanuella Magriplis, Themistoklis Dagklis, Michail Chourdakis
Summary: This study examined the relationship between maternal nutrition during pre-pregnancy and early pregnancy and the incidence of small-for-gestational-age neonates in women with gestational diabetes mellitus. The findings suggest that higher fat intake and lower intake of carbohydrates, fiber, magnesium, and copper during early pregnancy are associated with an increased risk of small-for-gestational-age neonates. These results emphasize the importance of assessing and modifying maternal nutrition in reducing the risk of gestational diabetes mellitus and its associated complications.
Article
Biochemistry & Molecular Biology
Yu-Jen Chen, Chia-Tien Hsu, Shang-Feng Tsai, Cheng-Hsu Chen
Summary: Chronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. In this study, the correlation between plasma miR-21 and the severity of CAD was investigated. RNA sequencing was performed and differentially expressed miRNAs in CAD were identified. MiR-21-5p, miR-20a-5p, and miR-101-3p were found to be associated with the TGF-beta pathway and CAD. These findings may lead to the development of early prediction miRNA biomarkers and therapeutic tools for CAD in the field of kidney transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Chen Yang, Zezhong Mou, Zheyu Zhang, Siqi Wu, Quan Zhou, Yiling Chen, Jian Gong, Chenyang Xu, Yuxi Ou, Xinan Chen, Xiyu Dai, Haowen Jiang
Summary: Our study revealed the tumor suppressive role of circRBPMS in bladder cancer by targeting the miR-330-3p/RAI2 axis, leading to inhibition of cell proliferation and metastasis. Downregulation of circRBPMS is associated with aggressive bladder cancer, while upregulation can suppress cell proliferation and migration.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Multidisciplinary Sciences
Masahide Harada, Daisuke Okuzaki, Akemi Yamauchi, Shiho Ishikawa, Yoshihiro Nomura, Asuka Nishimura, Yuji Motoike, Masayuki Koshikawa, Keisuke Hitachi, Kunihiro Tsuchida, Kentaro Amano, Atsuo Maekawa, Yasushi Takagi, Eiichi Watanabe, Yukio Ozaki, Hideo Izawa
Summary: This study aimed to identify cardiac-specific circulating miRNAs as biomarkers for atrial fibrillation (AF). The researchers found that miR-20b-5p and miR-330-3p can serve as cardiac-specific biomarkers for atrial remodeling progression and arrhythmia recurrence after catheter ablation in AF patients.
Article
Biotechnology & Applied Microbiology
Li Cai, Lu Ye, Xiaoqing Hu, Wenfeng He, Debao Zhuang, Qi Guo, Kuanyong Shu, Youkun Jie
Summary: Previous studies have suggested that miR-330-3p plays a role in various cancers, but its potential functions in ovarian cancer (OC) remained unclear. In this study, we found that miR-330-3p was downregulated in OC tissues and cell lines, and overexpression of miR-330-3p inhibited the proliferation, migration, and invasion of OC cells by targeting RIPK4, indicating that miR-330-3p may serve as a novel therapeutic target for OC treatment.
Article
Endocrinology & Metabolism
Ali Abbara, Maya Al-Memar, Maria Phylactou, Elisabeth Daniels, Bijal Patel, Pei C. Eng, Rans Nadir, Chioma Izzi-Engbeaya, Sophie A. Clarke, Edouard G. Mills, Tia Hunjan, Ewa Pacuszka, Lisa Yang, Paul Bech, Tricia Tan, Alexander N. Comninos, Tom W. Kelsey, Christopher Kyriacou, Hanine Fourie, Tom Bourne, Waljit S. Dhillo
Summary: Our study aimed to evaluate the changes in circulating kisspeptin levels in women with antenatal complications. The results showed that third-trimester kisspeptin levels were higher in women with hypertensive disorders of pregnancy (HDP) but lower in those with fetal growth restriction (FGR). We found that the odds of HDP increased by 30% and the odds of FGR decreased by 28% for every 1 nmol/L increase in plasma kisspeptin.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Cardiac & Cardiovascular Systems
Thomas P. Mullins, Kate I. Tomsett, Linda A. Gallo, Leonie K. Callaway, H. David McIntyre, Marloes Dekker Nitert, Helen L. Barrett
Summary: This study investigated the gut microbiota of overweight and obese women with gestational diabetes mellitus (GDM) compared to matched euglycaemic women at 16 and 28 weeks' gestation. The results showed minor variations in the gut microbiota of women with GDM compared to euglycemic women, suggesting that the microbiota likely does not have a disease-specific characterization in GDM.
NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES
(2021)
Article
Environmental Sciences
Maryam Rezaei, Martyna Blaszczyk, Alexey A. Tinkov, Lukasz J. Binkowski, Borhan Mansouri, Anatoly Skalny, Namamali Azadi, Monica Daniela Dosa, Geir Bjorklund
Summary: The study found that concentrations of arsenic, cadmium, and mercury in pregnant women are associated with the incidence of GDM, while intake of vanadium helps reduce the probability of GDM. Classification based on element levels can effectively differentiate between GDM patients and healthy individuals, suggesting that systematic monitoring of trace elements and multivariate modeling can help prevent the development of GDM.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Medicine, General & Internal
Yunkun Zhang, Chunmei Guo, Siwen Yang, Maroua Elkharti, Rui Liu, Ming-Zhong Sun, Shuqing Liu
Summary: After analyzing data from TCGA and GEO databases, we found that miR-330-3p may function as a tumor promoter in breast cancer, while EREG could act as a tumor suppressor. In 50 breast cancer tissues, miR-330-3p was upregulated, while lnc021545 and EREG were downregulated. MiR-330-3p promoted the metastatic behavior of breast cancer cells, while lnc021545 and EREG had the opposite effects.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Genetics & Heredity
Erbo Shan, Yuanyuan Yu, Wenbo Tang, Wei Wang, Xiangkui Wang, Shaobo Zhou, Yong Gao
Summary: This study predicts that miR-330-3p may regulate AQP9 in atherosclerosis (AS) through bioinformatics analysis. An AS model was established using ApoE(-/-) mice with a high-fat diet. Results showed that miR-330-3p expression decreased while AQP9 expression increased in the AS model. Overexpression of miR-330-3p or down-regulation of AQP9 reduced cell apoptosis and promoted cell proliferation and migration. The dual-luciferase reporter assay confirmed that AQP9 was directly inhibited by miR-330-3p. These findings suggest that miR-330-3p inhibits AS by regulating AQP9 and may serve as a new therapeutic target.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Gastroenterology & Hepatology
Manuela Salvucci, Nyree Crawford, Katie Stott, Susan Bullman, Daniel B. Longley, Jochen H. M. Prehn
Summary: The study identifies CMS4/CRIS-B patients with high Fn/Fusobacteriales prevalence as a high-risk subpopulation that may benefit from therapeutics targeting mesenchymal biology.
Article
Biochemical Research Methods
John Graf, Sanghee Cho, Elizabeth McDonough, Alex Corwin, Anup Sood, Andreas Lindner, Manuela Salvucci, Xanthi Stachtea, Sandra Van Schaeybroeck, Philip D. Dunne, Pierre Laurent-Puig, Daniel Longley, Jochen H. M. Prehn, Fiona Ginty
Summary: The study presented a new immunoFLuorescence Image NOrmalization method and evaluated it against alternative methods using multiround immunofluorescence staining with DAPI. Comparison of multiple normalization methods showed that upper quartile normalization of grid objects in log space had nearly equivalent performance to normalizing segmented cell objects by the middle quantile.
Article
Biochemistry & Molecular Biology
Andreas Ulrich Lindner, Manuela Salvucci, Elizabeth McDonough, Sanghee Cho, Xanthi Stachtea, Emer P. O'Connell, Alex D. Corwin, Alberto Santamaria-Pang, Steven Carberry, Michael Fichtner, Sandra Van Schaeybroeck, Pierre Laurent-Puig, John P. Burke, Deborah A. McNamara, Mark Lawler, Anup Sood, John F. Graf, Markus Rehm, Philip D. Dunne, Daniel B. Longley, Fiona Ginty, Jochen H. M. Prehn
Summary: The study utilized multiplexed immunofluorescence analysis to quantify 18 cell lineage and apoptosis proteins in different cells, revealing differences in apoptosis sensitivity. Single-cell resolution systems modeling identified enhanced sensitivity of cancer cells to mitochondrial permeabilization and executioner caspase activation compared to immune and stromal cells, with significant inter- and intra-tumor heterogeneity.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Pathology
Xanthi Stachtea, Maurice B. Loughrey, Manuela Salvucci, Andreas U. Lindner, Sanghee Cho, Elizabeth McDonough, Anup Sood, John Graf, Alberto Santamaria-Pang, Alex Corwin, Pierre Laurent-Puig, Sonali Dasgupta, Jinru Shia, Jonathan R. Owens, Samantha Abate, Sandra Van Schaeybroeck, Mark Lawler, Jochen H. M. Prehn, Fiona Ginty, Daniel B. Longley
Summary: In this study, immune signatures in the tumor and tumor microenvironment were assessed using a multiplexed immunofluorescence imaging technology, revealing a significant association between regulatory T cells (Tregs) and survival rates in colorectal cancer patients. Multi-marker classification provided more accurate quantitation of immune cell types with stronger correlations with patient outcomes compared to single markers.
Article
Oncology
Vera Grinkevitch, Mark Wappett, Nyree Crawford, Stacey Price, Andrea Lees, Christopher McCann, Katherine McAllister, Jochen Prehn, Jamie Young, Jess Bateson, Lewis Gallagher, Magali Michaut, Vivek Iyer, Aikaterini Chatzipli, Syd Barthorpe, Daniel Ciznadija, Ido Sloma, Amy Wesa, David A. Tice, Lodewyk Wessels, Mathew Garnett, Daniel B. Longley, Ultan McDermott, Simon S. McDade
Summary: Second-generation TRAIL-R2 agonists face two major clinical challenges: lack of predictive biomarkers and development of resistance. This study identifies caspase-8:FLIP(L) and caspase-8:MCL-1 ratios as potential predictive biomarkers for second-generation TRAIL-R2 agonists, and loss of key effectors like FADD and caspase-8 as likely drivers of clinical resistance in solid tumors.
MOLECULAR CANCER THERAPEUTICS
(2022)
Review
Cell Biology
Sandeep Chenna, Werner J. H. Koopman, Jochen H. M. Prehn, Niamh M. C. Connolly
Summary: This article discusses the mechanistic models and minimal models of mitochondrial reactive oxygen species (ROS) homeostasis. Mechanistic models provide valuable insights but are complex, while minimal models use a smaller set of equations to describe empirical relationships and generate hypotheses. Finally, the article illustrates the application of minimal models in studying the pathophysiology of ROS.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Laura Gomez-Jaramillo, Fatima Cano-Cano, Maria del Carmen Gonzalez-Montelongo, Antonio Campos-Caro, Manuel Aguilar-Diosdado, Ana Arroba
Summary: This review focuses on the effects of peripheral tissues in Huntington's disease and identifies biomarkers and mechanisms as new therapeutic or early diagnostic options. The article describes the specific changes in peripheral tissues such as serum/plasma, blood cells, pancreas, heart, retina, liver, kidney and pericytes as a part of the blood-brain barrier.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
William P. Duggan, Emer O'Connell, Jochen H. M. Prehn, John P. Burke
Summary: Alternative splicing, regulated by SRPK1, is implicated in cancer progression and poor survival. Increased SRPK1 expression is associated with proliferation, invasion, migration, and apoptosis in vitro, as well as tumor growth, tumorigenicity, and metastasis in vivo. Aberrant SRPK1 expression is involved in multiple signaling pathways associated with oncogenesis. SRPK1-targeting microRNAs play a role in regulating SRPK1 activity. SRPK1 expression is closely related to the response of tumors to platinum-based chemotherapy.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Alejandro Garcia-Nunez, Gema Jimenez-Gomez, Antonio Hidalgo-Molina, Juan Antonio Cordoba-Dona, Antonio Leon-Jimenez, Antonio Campos-Caro
Summary: This study investigates circulating biomarker levels and systemic inflammatory indices in patients with silicosis caused by occupational exposure to engineered stone. The study finds that levels of lactate dehydrogenase, angiotensin-converting-enzyme, and fibrinogen show a significant sequential increase in different stages of the disease. Systemic inflammation indices also show significant differences between patient groups. These biomarkers and indices can help predict the progression of silicosis.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Aurore Tourville, David Akbar, Olga Corti, Jochen H. M. Prehn, Ronald Melki, Stephane Hunot, Patrick P. Michel
Summary: The researchers established a model system to study alpha-Synuclein aggregation and neurodegeneration in Parkinson's disease. They found that F91 fibrils have a strong propensity to induce aggregation in DA neurons, leading to subsequent loss of these neurons. This neuronal loss can be prevented by treatment with GDNF.
Article
Oncology
Hajrah Khawaja, Rebecca Briggs, Cheryl H. Latimer, Mustasin Rassel, Dary Griffin, Lyndsey Hanson, Alberto Bardelli, Frederica Di Nicolantonio, Simon S. McDade, Christopher J. Scott, Shauna Lambe, Manisha Maurya, Andreas U. Lindner, Jochen H. M. Prehn, Jose Sousa, Chris Winnington, Melissa J. LaBonte, Sarah Ross, Sandra Van Schaeybroeck
Summary: Novel covalent inhibitors of KRASG12C have shown limited response rates in patients with KRASG12C-mutant colorectal cancer. In this study, researchers found that the combination of AZ'1569 and a Bcl-xL inhibitor led to a dramatic and universal apoptosis, suggesting a potential therapeutic strategy for patients with KRAS G12C-mutant colorectal cancer.
MOLECULAR CANCER THERAPEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Antonio Campos-Caro, Gema Jimenez-Gomez, Alejandro Garcia-Nunez, Antonio Hidalgo-Molina, Antonio Leon-Jimenez
Summary: Engineered stone silicosis is a rapidly progressing occupational epidemic disease that leads to respiratory failure and death, with no effective treatment available. This study investigated differences in plasma cytokine levels between patients with simple silicosis (SS) and progressive massive fibrosis (PMF). The results showed that multiple cytokines were elevated in silicosis patients compared to healthy controls, and several cytokines were significantly higher in PMF patients compared to SS patients. These findings suggest that plasma cytokine levels may be involved in disease severity and could be potential biomarkers and therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Florian Pichot, Marion C. Hogg, Virginie Marchand, Valerie Bourguignon, Elisabeth Jirstrom, Cliona Farrell, Hesham A. Gibriel, Jochen H. M. Prehn, Yuri Motorin, Mark Helm
Summary: Modification of tRNA is crucial for generating RNA expression diversity. In this study, the stoichiometry of incompletely modified sites in human tRNAs was investigated, and it was found that up to 75% of sites can be incompletely modified. The modification patterns of tRNA populations can provide information to differentiate different cell lines.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Endocrinology & Metabolism
N. Ng, H. A. Y. Gibriel, L. Halang, E. Jirstrom, J. A. Ioana, M. Burke, M. M. Byrne, J. H. M. Prehn
Summary: This study investigated the levels of tiRNA 5'ValCAC (alone and in combination with miR-23b-3p) in serum samples from healthy controls, type 1 diabetes, type 2 diabetes, and MIDD subjects. The results showed reduced levels of 5'ValCAC in MIDD and type 2 diabetes subjects compared to controls, and higher levels of miR-23b-3p in type 2 diabetes subjects. Receiver Operating Characteristic analysis demonstrated the potential of 5'ValCAC and miR-23b-3p as MIDD biomarkers, with the combination showing excellent separation from type 2 diabetes subjects.
Meeting Abstract
Oncology
Hajrah Khawaja, Rebecca Briggs, Cheryl Latimer, Md A. M. B. Rassel, Daryl Griffin, Lyndsey Hanson, Alberto Bardelli, Federica Di Nicolantonio, Simon McDade, Christopher Scott, Shauna Lambe, Manisha Maurya, Andreas Ulrich Lindner, Jochen H. M. Prehn, Jose Sousa, Chris Winnington, Melissa J. LaBonte, Sarah Ross, Sandra Van Schaeybroeck
JOURNAL OF CLINICAL ONCOLOGY
(2022)