Article
Genetics & Heredity
Yasunori Hino, Md Mahfuzur Rahman, Yu-Chang Lai, Al Asmaul Husna, Hui-wen Chen, Md Nazmul Hasan, Takayuki Nakagawa, Naoki Miura
Summary: In this study, hypoxia-regulated miRNAs in canine oral melanoma were identified through next generation sequencing and qRT-PCR. The research revealed differences in the regulation and response of hypoxic miRNAs between primary and metastatic melanoma cells. Additionally, miR-21 and miR-301a were found to control a complex gene regulatory network in response to hypoxia.
Article
Chemistry, Multidisciplinary
Na Zhang, An-di Sun, Si-man Sun, Rui Yang, Yan-yan Shi, Qi-long Wang, Xin-yu Li, Ji-hong Ma, Wei Yue, Bing-teng Xie, Jie Qiao, Mo Li
Summary: Oocyte mitochondria play a crucial role in germ cell maturation and the study successfully identified 158 proteins in mouse oocyte mitochondria using a novel technology. The proteomic analysis revealed the importance of these proteins in processes such as cellular respiration and ATP metabolism. Furthermore, exposure to the chemotherapeutic drug cisplatin led to differential changes in the abundance of oocyte-specific mitochondrial proteins, highlighting dynamic shifts in protein abundance associated with chemotherapeutic agents.
ACTA PHARMACOLOGICA SINICA
(2021)
Article
Cell Biology
Dian Lu, Rui Liu, Yuting Zhou, Zhenbo Zhang, Xiuqin Jiang, Airong Su, Zhenzhen Hu
Summary: In this study, the researchers found that hypoxia activates mitophagy and decreases cisplatin sensitivity in human osteosarcoma cells through the FOXO3a/HSP90/Ulk1/FUNDC1 signaling pathway.
CELLULAR SIGNALLING
(2023)
Article
Chemistry, Multidisciplinary
Fan Tang, Yan Tie, Tian-Xia Lan, Jing-Yun Yang, Wei-Qi Hong, Si-Yuan Chen, Hou-Hui Shi, Long-Qing Li, Hao Zeng, Li Min, Yu-Quan Wei, Chong-Qi Tu, Xia-Wei Wei
Summary: The major challenge in treating osteosarcoma patients is the metastatic disease, particularly in the lungs. However, the underlying mechanism of recurrence and metastasis after surgical resection of primary tumor is unclear. This study investigates the association between pulmonary metastases in osteosarcoma and surgical treatment, finding that surgery contributes to the formation of a pre-metastatic niche in the lung tissue. It is demonstrated that circulating tumor cells, neutrophils, and myeloid cells contribute to this niche formation through molecular patterns released from surgical resection.
Article
Multidisciplinary Sciences
Silje Kjlle, Kenneth Finne, Even Birkeland, Vandana Ardawatia, Ingeborg Winge, Sura Aziz, Gril Knutsvik, Elisabeth Wik, Joao A. Paulo, Heidrun Vethe, Dimitrios Kleftogiannis, Lars A. Akslen
Summary: This study aims to investigate the response of breast cancer cell lines to hypoxia and identify the associated protein features. The identified features can predict patient survival and have significant prognostic implications even in low-grade tumors.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Giulia Alloisio, David Becerril Rodriguez, Marco Luce, Chiara Ciaccio, Stefano Marini, Antonio Cricenti, Magda Gioia
Summary: This study used uniaxial-stretching technology to select a mechanical regimen that can increase the migration of SAOS-2 cells in osteosarcoma. Through confocal and atomic force microscopy, it was found that a 24 h 1 Hz cyclic elongation can induce morphological changes in cells, as well as changes in cell adhesion and migration properties. These changes are independent of osteoblastic differentiation grade.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Ines Godet, Mahelet Mamo, Andrea Thurnheer, D. Marc Rosen, Daniele M. Gilkes
Summary: Intratumoral hypoxia in breast cancer leads to metastasis and chemotherapy resistance. Cells exposed to hypoxia in the primary tumor show decreased sensitivity to doxorubicin and paclitaxel, along with enrichment in cancer stem cell-related pathways. These post-hypoxic cells contribute to resistance and metastatic recurrence.
Article
Cell Biology
Qing He, Changzhi Yu, Yang Li, Peng Hao, Hantao Mai, Ruilian Guo, Guifang Zhong, Kelin Zhang, Chipiu Wong, Qian Chen, Yantao Chen
Summary: Chemotherapy resistance is a significant problem in osteosarcoma, and this study investigates the role of histone deacetylases (HDACs) in chemoresistance. HDAC6 is found to be upregulated in resistant cells and its inhibition using a specific inhibitor restores chemosensitivity. HDAC6 interacts with ERR alpha to regulate its acetylation and stability, and inhibition of ERR alpha further enhances chemosensitivity. K129 acetylation is identified as a key mechanism for HDAC6-regulated levels of ERR alpha. Overall, targeting the HDAC6/ERR alpha axis may be a potential strategy to overcome chemoresistance in osteosarcoma treatment.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Cell Biology
Martina Quartieri, Anggraeini Puspitasari, Tamara Vitacchio, Marco Durante, Walter Tinganelli
Summary: This study investigates the interplay between acute and chronic hypoxia and radiation exposure in selecting and characterizing cells with a Circulating Cancer Stem Cell (CCSC)-like phenotype. The results demonstrate that the combination of radiation and acute hypoxia enhances stemness, while chronic hypoxia imparts a cancer stem-like phenotype in cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Oncology
Jinluan Lin, Xinwu Wang, Xinwen Wang, Shenglin Wang, Rongkai Shen, Yanbing Yang, Jianyong Xu, Jianhua Lin
Summary: Hypoxia can induce stem cell-like phenotypes in some osteosarcoma cells, with increased expression of CSC markers and enhanced sphere formation and xenograft tumor growth. Rapamycin, by suppressing HIF-1 alpha protein levels, inhibits the hypoxic response and reduces stem cell marker expression, leading to decreased sphere and xenograft tumor formation efficiency in osteosarcoma cells.
Article
Oncology
Moritz von Fallois, Friederike Katharina Kosyna, Markus Mandl, Yosef Landesman, Jurgen Dunst, Reinhard Depping
Summary: CRM1, as an overexpressed nuclear export receptor in cancer, can be selectively inhibited by Selinexor, a new FDA-approved drug. Selinexor significantly reduces the intranuclear level of HIF-1 alpha protein and enhances radiosensitivity in cancer cells, suggesting that targeting the HIF pathway by Selinexor could be a promising approach to overcome hypoxia-induced radioresistance.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2021)
Article
Oncology
Shang-You Yang, Elka Garcia, Will Xia, Anyu Wang
Summary: This study found that HIF-1 alpha and VEGF play important roles in regulating osteosarcoma cell growth and apoptosis under hypoxic conditions, with a close relationship between the two. Blocking HIF-1 alpha or VEGF can exacerbate cell growth retardation and promote cell apoptosis.
ANTICANCER RESEARCH
(2021)
Article
Oncology
Yangbo Xu, Yafei Li, Xiaofan Chen, Feifan Xiang, Yong Deng, Zhong Li, Daiqing Wei
Summary: The study showed that TGF-beta reduced SDH expression by decreasing the transcription factor STAT1, leading to the upregulation of HIF1 alpha, altering glucose metabolism, and exacerbating chemoresistance in osteosarcoma cells. This connection between tumor cell metabolism and chemotherapy resistance may provide insights for developing additional treatments for osteosarcoma.
Article
Genetics & Heredity
Junlei Zhang, Haijun Tang, Xiaohong Jiang, Nenggan Huang, Qingjun Wei
Summary: The study found that high expression of BYSL in osteosarcoma tissues was positively associated with metastasis and poor prognosis. Mechanistically, upregulation of BYSL enhanced Nrf2 expression under hypoxia in osteosarcoma cells. In addition, miR-378a-3p was found to inhibit BYSL expression by binding to its 3'-untranslated region and suppress osteosarcoma cell invasion and epithelial-to-mesenchymal transition.
FRONTIERS IN GENETICS
(2022)
Article
Pharmacology & Pharmacy
Yapeng Hou, Yan Ding, Danni Du, Tong Yu, Wei Zhou, Yong Cui, Hongguang Nie
Summary: Hypoxia in the airway can induce epithelial-mesenchymal transition, which may be mediated by basal cells in the airway epithelium.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Veterinary Sciences
Margaret E. Duckett, Kaitlin M. Curran, Haley J. Leeper, Carl E. Ruby, Shay Bracha
Summary: The study showed that fasting can significantly reduce nausea, anorexia, lethargy, and serum insulin levels in dogs receiving vincristine treatment, indicating that fasting may be a safe and effective treatment modality to mitigate adverse events in tumor-bearing dogs.
VETERINARY AND COMPARATIVE ONCOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Elise M. Van Fossen, Sonia Grutzius, Carl E. Ruby, Dan V. Mourich, Chris Cebra, Shay Bracha, P. Andrew Karplus, Richard B. Cooley, Ryan A. Mehl
Summary: In this study, we demonstrate the development of a selective nanobody using genetic code expansion technology, which provides a new tool for investigating oxidative stress-related pathologies.
FRONTIERS IN CHEMISTRY
(2022)
Article
Cell Biology
Daphne R. Mattos, Marcus A. Weinman, Xuemei Wan, Cheri P. Goodall, Jeffrey D. Serrill, Kerry L. McPhail, Milan Milovancev, Shay Bracha, Jane E. Ishmael
Summary: This study found that canine osteosarcoma cells exhibited stress adaptation and were more sensitive to inhibitors of Grp78 function compared to normal cells. Inhibition of Grp78, Hsp70, and Hsp90 function may have therapeutic effects against carboplatin-resistant osteosarcoma cells.
CELL STRESS & CHAPERONES
(2022)
Article
Statistics & Probability
Canhong Wen, Qin Wang, Yuan Jiang
Summary: The reduced-rank regression model is widely used in various fields to handle multivariate response and multiple predictors. The estimation of the rank of the coefficient matrix is a key objective in reduced-rank regression modeling. This paper proposes a novel method called StARS-RRR, which selects the tuning parameter and estimates the rank based on stability approach. Theoretical analysis proves that StARS-RRR achieves rank estimation consistency. Simulation study demonstrates that StARS-RRR outperforms other tuning parameter selection methods and exhibits a smaller prediction error in a breast cancer dataset.
JOURNAL OF COMPUTATIONAL AND GRAPHICAL STATISTICS
(2023)
Article
Nutrition & Dietetics
Helen Holvoet, Dani M. Long, Alexander Law, Christine McClure, Jaewoo Choi, Liping Yang, Luke Marney, Burkhard Poeck, Roland Strauss, Jan F. Stevens, Claudia S. Maier, Amala Soumyanath, Doris Kretzschmar
Summary: Withania somnifera (WS) extracts, widely used in traditional medicine and as nutritional supplements, have shown positive effects on healthy aging, sleep, and resilience against stress. Different extracts may have different beneficial effects due to the variation in active ingredients.
Article
Statistics & Probability
Chuan Tian, Duo Jiang, Austin Hammer, Thomas Sharpton, Yuan Jiang
Summary: Understanding microbiota interactions is crucial for understanding their impact on the host or environment. We introduce a novel method called compositional graphical lasso that explicitly incorporates the unique characteristics of microbiome data and outperforms existing methods in accurately resolving microbial interactions.
JOURNAL OF THE AMERICAN STATISTICAL ASSOCIATION
(2023)
Article
Biochemistry & Molecular Biology
Caroline K. Glidden, Canan Karakoc, Chenyang Duan, Yuan Jiang, Brianna Beechler, Abdul Jabbar, Anna E. Jolles
Summary: Individual animals in natural populations tend to host diverse parasite species concurrently over their lifetimes. In free-living ecological communities, organismal life histories shape interactions with their environment, which ultimately forms the basis of ecological succession. Here, we studied community dynamics of 12 subtypes of protozoan microparasites (Theileria spp.) in a herd of African buffalo.
Article
Genetics & Heredity
McKaela A. Hodge, Tasha Miller, Marcus A. Weinman, Brandan Wustefeld-Janssens, Shay Bracha, Brian W. Davis
Summary: The prognosis of osteosarcoma has not improved in the past three decades. Current treatments are limited to surgery, radiation, and chemotherapy. Chemoresistance is the main reason for treatment failure, and the tumor evolution trajectory under treatment pressure is believed to be a major contributing factor. By studying cells subjected to repeated cycles of treatment with increasing carboplatin concentrations, we investigated the nature of platinum-based chemotherapy resistance. Our findings indicate that acquired chemoresistance is strongly associated with a process similar to epithelial-mesenchymal transition (EMT) and this EMT-like phenotype plays a critical role in tumor evolution and chemoresistance. Understanding the mechanisms of chemoresistance and the role of EMT-like phenotype is crucial for improving osteosarcoma prognosis.
Article
Plant Sciences
Michael F. Nagle, Jialin Yuan, Damanpreet Kaur, Cathleen Ma, Ekaterina Peremyslova, Yuan Jiang, Bahiya Zahl, Alexa Nino de Rivera, Wellington Muchero, Li Fuxin, Steven H. Strauss
Summary: Adventitious rooting (AR) plays a crucial role in tree propagation, breeding, and genetic engineering. However, the genetic basis of AR is not well-characterized. To identify genetic regulators of AR, a genome-wide association study (GWAS) was performed using 1148 Populus trichocarpa genotypes. A computer vision system was developed to measure various traits related to adventitious root development. GWAS analysis revealed 277 unique associations, indicating that genes involved in hormone signaling, cell division, and other root development processes play a role in AR. Numerous genes with unknown functions were also identified as candidates for further analysis.
HORTICULTURE RESEARCH
(2023)
Review
Veterinary Sciences
Jeilene N. Hambly, Carl E. Ruby, Dan V. Mourich, Shay Bracha, Brian P. Dolan
Summary: Immunotherapy for cancer treatment aims to use the body's immune system to eliminate tumor cells. While successful in human medicine, it is unknown whether the available biological therapies designed for humans are effective in veterinary medicine. This article reviews the potential use of these therapies in veterinary oncology and explores other possible options.
VETERINARY SCIENCES
(2023)
Article
Physics, Atomic, Molecular & Chemical
Liping Yang, Stanislau Stanisheuski, Zifeng Song, Shay Bracha, Claudia S. Maier
Summary: A top-down mass spectrometry approach was developed and evaluated to detect and quantify low molecular weight proteins in osteosarcoma cells. The findings showed a correlation between protein levels and the metastatic phenotypes of osteosarcoma, suggesting the potential of small molecular weight proteins as biomarkers.
EUROPEAN JOURNAL OF MASS SPECTROMETRY
(2023)
Article
Nutrition & Dietetics
Helen Holvoet, Dani M. Long, Liping Yang, Jaewoo Choi, Luke Marney, Burkhard Poeck, Claudia S. Maier, Amala Soumyanath, Doris Kretzschmar, Roland Strauss
Summary: This study investigates the potential protective role of Centella asiatica (CA) in depression using a Drosophila model. The water extract of CA was found to improve depressive symptoms and mono-caffeoylquinic acids were identified as the active compounds mediating this effect. The protective function of CA is due to a synergy between chlorogenic acid and one of its isomers, and chlorogenic acid acts through calcineurin, a multifunctional phosphatase involved in neuronal maintenance.
Article
Nanoscience & Nanotechnology
Cheri Peyton Goodall, Benjamin Schwarz, Ekaterina Selivanovitch, John Avera, Joseph Wang, Heini Miettinen, Trevor Douglas
Summary: Ligands of the TNFSF are promising targets for immunotherapy research, with multivalency playing a crucial role in enhancing signaling effects and providing new insights into ligand-receptor interactions.
ACS APPLIED BIO MATERIALS
(2021)
Article
Mathematical & Computational Biology
Kai Li, Meng Mei, Yuan Jiang
Summary: The study introduces a novel composite group penalization, prior group bridge, which retains important groups and selects important variables simultaneously, demonstrating its efficacy in predictive factor research with prior information.
STATISTICS AND ITS INTERFACE
(2021)
Article
Mathematical & Computational Biology
Duo Jiang, Thomas Sharpton, Yuan Jiang
Summary: This paper introduces a new network estimation method, BC-GLASSO, for analyzing microbiome data. It corrects bias in the model and builds the inverse covariance estimator via graphical lasso. Experimental results show that BC-GLASSO has advantages over current methods in microbial interaction network estimation.
STATISTICS IN BIOSCIENCES
(2021)