Article
Public, Environmental & Occupational Health
Marina D'Amato, Przemyslaw Szostak, Benjamin Torben-Nielsen
Summary: Whole slide images (WSIs) are digitized histopathology images stored in a pyramidal data structure with different magnification levels, revealing relevant properties. With high resolution, WSIs are broken down into tiles for processing, and multiple scale analysis using multiple instance learning and barcode approach improves performance.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Mechanics
Yangyang Li, Huimin Xie
Summary: The virtual fields method (VFM) for single edge notched tension (SENT) specimens was developed in this study to simultaneously characterize the elastic constants and stress intensity factors (SIFs). By combining full-field measurements and virtual fields generated by the finite element method (FEM), this approach can satisfy complex boundary conditions and minimize the influence of the plastic zone near the crack tip.
ENGINEERING FRACTURE MECHANICS
(2023)
Article
Agriculture, Dairy & Animal Science
Ida Svetlicic, Dean Konjevic, Elena Buzan, Miljenko Bujanic, Luka Dunis, Suncica Stipoljev, Jelena Martincic, Mihaela Surina, Ana Galov
Summary: Major histocompatibility complex (MHC) genes are valuable markers in wildlife genetic studies due to their extreme polymorphism and functional importance. This study compares molecular cloning with Illumina and Ion Torrent NGS sequencing for MHC-DRB genotyping in single-locus and multi-locus species, aiming to adopt a reliable and straightforward method without complex bioinformatic analysis.
Article
Engineering, Multidisciplinary
Gabriel Yuji Garoli, Diogo Stuani Alves, Tiago Henrique Machado, Katia Lucchesi Cavalca, Helio Fiori de Castro
Summary: Fault identification is crucial in the field of rotating machines, enabling better maintenance of expensive equipment. Common faults like unbalance and bearing wear can be identified through deterministic or stochastic methods. The Bayesian inference with polynomial chaos showed promising results for reliable identification of fault parameters.
STRUCTURAL HEALTH MONITORING-AN INTERNATIONAL JOURNAL
(2021)
Article
Thermodynamics
Kari Lappalainen, Michel Piliougine, Giovanni Spagnuolo
Summary: This article proposes a novel procedure for identifying the single-diode model parameter values, as well as the operating irradiance and temperature values, from measured I-V curves of photovoltaic (PV) modules. The proposed procedure eliminates the need for other measurements and has been experimentally validated. The results show that it can accurately identify the operating conditions and reliably determine the series and shunt resistances under various irradiance conditions. Additionally, a comprehensive comparison of different fitting approaches based on RMSE minimization is presented.
ENERGY CONVERSION AND MANAGEMENT
(2022)
Article
Engineering, Environmental
Daniel F. Araujo, Emmanuel Ponzevera, Nicolas Briant, Joel Knoery, Sandrine Bruzac, Teddy Sireau, Anne Pellouin-Grouhel, Christophe Brach-Papa
Summary: The study demonstrates the potential of Cu isotopes in bivalves to infer Cu bioavailability changes related to anthropogenic inputs of this metal into the marine environment. Oysters and mussels displayed different Cu isotope variations over 10 years, reflecting their different bioaccumulation mechanisms and physiological features.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Food Science & Technology
Marianela Quintrel, Felipe Jilberto, Matias Sepulveda, Maria Elisa Marin, David Veliz, Cristian Araneda, Maria Angelica Larrain
Summary: The study developed a DNA-based method for species identification using SNP markers, which can be widely applied in the seafood industry with high accuracy and consistency.
Article
Plant Sciences
Lance F. Merrick, Adrienne B. Burke, Zhiwu Zhang, Arron H. Carter
Summary: This study aimed to dissect the genetic architecture of seedling emergence in wheat using one multi-trait genome-wide association study (MT-GWAS) and three single-trait GWAS (ST-GWAS) models. The ST-GWAS models identified 107 significant markers across 19 chromosomes, while the MT-GWAS identified 82 significant markers across 14 chromosomes. The FarmCPU and BLINK models were able to identify both small effect markers and large effect markers on chromosome 5A.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Environmental Sciences
Camille G. Baettig, Martin Zirngibl, Kirsty F. Smith, Gavin Lear, Louis A. Tremblay
Summary: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the current gold-standard technique for detecting and quantifying messenger RNA. Droplet digital PCR (ddPCR) allows for more sensitive and accurate measurements of targeted nucleic acids without external standards. This study compared these two PCR-based methods and concluded that ddPCR is suitable for assessing gene expression in an ecotoxicological context, while RT-qPCR is more time-efficient and cost-effective.
MARINE POLLUTION BULLETIN
(2023)
Article
Horticulture
Anastasia Boutsika, Georgia Tanou, Aliki Xanthopoulou, Martina Samiotaki, Irini Nianiou-Obeidat, Ioannis Ganopoulos, Ifigeneia Mellidou
Summary: In the era of climate change and degradation of culturable land, it is crucial to secure global food supply in a sustainable manner. High-throughput-omic technologies can help unravel the genetic mysteries underlying plant phenotypic diversity and plasticity, and mitigate the threats of climate change on agriculture.
SCIENTIA HORTICULTURAE
(2022)
Review
Biochemical Research Methods
Anson T. Ku, Scott Wilkinson, Adam G. Sowalsky
Summary: Intratumoral heterogeneity is a known feature of human cancers that affects the analysis of gene expression differences. Repeated sampling of the same tumor can increase the statistical power of gene expression analysis and improve understanding of intratumoral variance.
BRIEFINGS IN BIOINFORMATICS
(2021)
Review
Biochemical Research Methods
Tasbiraha Athaya, Rony Chowdhury Ripan, Xiaoman Li, Haiyan Hu
Summary: This article reviews the application of multimodal deep learning techniques in the integration of single-cell multi-omics data, providing insights and categorizing different methods based on data modality, deep learning architecture, fusion strategy, key tasks and downstream analysis.
BRIEFINGS IN BIOINFORMATICS
(2023)
Article
Ecology
Sidali Kourdali, Kamel Boudjema, Abdellah Meknachi, Nabila Bounakous, Bassem Jaouadi, Sondes Mechri, Abdelmalek Badis
Summary: The present research aims to evaluate the variation of several responses in marine mussels exposed to different sub-lethal concentrations of copper and lead. The results showed that temperature and salinity variations were not significant enough to interfere with the effect of the pollutants. Changes in nitrogen and phosphorus mineral matter were found to be a physiological disturbance in both species. During the contamination cycles, catalase induction and protein depletion reflected the oxidative stress caused by the metals. However, the catalase activity returned to normal state during the decontamination cycles.
REGIONAL STUDIES IN MARINE SCIENCE
(2022)
Article
Surgery
Yuchen Zhong, Tianyu Qiao, Tianyi Ma, Hanqing Hu, Yinghu Jin, Yuliuming Wang, Qingchao Tang, Rui Huang, Guiyu Wang, Xishan Wang
Summary: In this retrospective analysis, the reliability of natural orifice specimen extraction surgery (NOSES) for middle rectal cancer treatment was evaluated through comparing it with conventional laparoscopic-assisted resection (LA). The short-term outcomes of NOSES, including surgical bleeding volume, time to first flatus, and time to regular diet, were significantly better than those of LA, while the long-term overall survival (OS) and disease-free survival (DFS) did not differ between the two surgical approaches.
SURGICAL INNOVATION
(2021)
Article
Environmental Sciences
L. De Marchi, L. R. Vieira, L. Intorre, V Meucci, F. Battaglia, C. Pretti, A. M. V. M. Soares, R. Freitas
Summary: This study investigated the combined effects of marine heatwaves and caffeine toxicity on two species of bivalves, revealing alterations in neurotransmission, detoxification mechanisms, and cellular damage under simulated marine heatwaves. The results suggest an increase in antioxidant defense mechanisms and metabolic activity, as well as a decrease in energy reserves.
Article
Biodiversity Conservation
Andre Lasalle, Pablo Caceres, Tamara Montenegro, Cristian Araneda, Jose Yanez, Denise Vizziano-Cantonnet
Summary: Researchers have generated a high-quality SNP panel from female and male sturgeons through high-depth RAD-seq, which can facilitate genetic studies and improve caviar production.
CONSERVATION GENETICS RESOURCES
(2022)
Article
Fisheries
Pablo Gallardo, Guilherme Wolff Bueno, Cristian Araneda, Tillmann Benfey
Summary: The European aquaculture industry started Atlantic halibut aquaculture in the 1980s due to its favorable growth rate and high market value. After 12 years and a total investment of $2 million USD, Chilean aquaculture facilities were finally equipped to rear Atlantic halibut. Infrastructure is now in place to support the development of a sustainable marine aquaculture industry for this species.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Roman Wenne, Anna Pradzinska, Anita Pocwierz-Kotus, Maria Angelica Larrain, Cristian Araneda, Malgorzata Zbawicka
Summary: This study assessed the species and geographical origin information labeled on mussel products available in European stores and markets using a genetic approach. The results showed that most of the information on the food labels was consistent with the genetic tools, but incomplete and incorrect information was also found. The study confirmed that a multi-locus approach based on multiple SNPs is needed for accurate species identification, and a reduced panel of 34 SNP loci can be widely used for this purpose.
Article
Fisheries
Ane del Rio Lavin, Natalia Diaz-Arce, Maria Angelica Larrain, Cristian Araneda, Naiara Rodriguez-Ezpeleta, Elisa Jimenez, Miguel Angel Pardo
Summary: Seafood traceability is important for regulators and fishing industries to prevent fraud and ensure consumer safety. This study used genetic markers to trace the genetic structure of Mediterranean mussel and assess the ability of new markers to trace the origin of samples. The results showed clear differentiation between populations from different regions and suggested the potential for developing an accurate and cost-effective traceability tool.
Article
Food Science & Technology
Pablo Dufflocq, Maria Angelica Larrain, Cristian Araneda
Summary: Research in Chile identified food fraud incidents in the swordfish market, with vulnerable shark species being mislabeled or substituted. It is recommended to raise stakeholder awareness on shark conservation, establish specific labeling regulations, and enforce them effectively with DNA-based methods.
Article
Food Science & Technology
Alice Giusti, Chiara Malloggi, Federica Tosi, Pierfrancesco Boldini, Maria Angelica Larrain Barth, Cristian Araneda, Giuseppe Arcangeli, Andrea Armani
Summary: The study evaluated the labelling compliance of market products containing Mytilus spp. in Italy with EU and national legislation. The PCR-RFLP technique was used to accurately discriminate between different Mytilus species, showing high compliance with labelling regulations and potential for further improvement in identifying species and hybrids.
Article
Clinical Neurology
Alejandra Zazueta, Tito Castillo, Alvaro Cavieres, Rene Gonzalez, Maximiliano Abarca, Rodrigo R. Nieto, Javier Deneken, Cristian Araneda, Pablo R. Moya, M. Leonor Bustamante
Summary: The response to conventional antipsychotic drugs and clozapine treatment is associated with genetic variations, but more research is needed for personalized prescriptions.
INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY
(2022)
Article
Fisheries
Pedro Baez, Cristian Araneda, Ludavic Burns, Claudia Navarrete
Summary: A specimen of Scyllarides haanii, a species of slipper lobster, was found in the coastal vicinity of Ahu Tongariki, Rapa Nui. The taxonomic determination was conducted through morphological examination and DNA barcoding analysis. This finding extends the geographic distribution of S. haanii to Rapa Nui and increases the total number of marine lobster species on the island to five.
LATIN AMERICAN JOURNAL OF AQUATIC RESEARCH
(2022)
Article
Food Science & Technology
Carmen E. Vargas-Peralta, Cristian Araneda, Clara E. Galindo-Sanchez, M. Angelica Larrain, Miguel A. Del Rio-Portilla, Fabiola Lafarga-De La Cruz
Summary: This study analyzed the genetic data of five abalone species using RAD-seq and developed markers for traceability and specimen identification, providing a basis for fishery management measures in abalone trade.
Editorial Material
Physiology
Jianlong Ge, Yu-Hung Lin, Cristian Araneda, Qingchao Wang
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Felipe Jilberto, Malgorzata Zbawicka, Roman Wenne, Maria Angelica Larrain, Cristian Araneda
Summary: This study investigates the genetic species composition and admixture in the natural hybrid zone between M. chilensis and M. platensis in the southern end of South America using SNP markers. The analysis reveals a clinal architecture in the hybrid zone, with different areas showing varying proportions of pure individuals and hybrids. The study also does not find evidence of other mussel species in the zone.
Article
Food Science & Technology
Cynthia M. Asorey, Felipe Jilberto, Ilka Haase, Rainer Schubbert, Maria Angelica Larrain, Cristian Araneda
Summary: Seafood international trade has led to stricter labeling requirements, including the need for product information to help traders and consumers make informed choices. The EU Regulation imposes the declaration of official commercial and scientific names for all fishery and aquaculture products, enforced through DNA analyses. This study compares two mono-locus approaches for species identification in mussels, highlighting the need for standardized molecular tools and multi-locus methods.
FOOD CHEMISTRY: MOLECULAR SCIENCES
(2022)
