Journal
ONCOLOGY LETTERS
Volume 19, Issue 3, Pages 2258-2264Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/ol.2020.11352
Keywords
5-Fluorouracil; gastric cancer; drug sensitivity; RPL11; P53 pathway
Categories
Funding
- JSPS KAKENHI [JP17J05291, JP15K10311, JP15K10338, JP22501047, JP16K07121, JP17K10871, JP17K07221, JP18K06732]
- Molecular Profiling Committee
- Ministry of Education, Culture, Sports, Science and Technology, Japan (JSPS KAKENHI) [JP 16H06276]
- Takeda Medical Foundation
- Shinnihon Foundation of Advanced Medical Treatment Research
- Kodama Memorial Fund for Medical Research
- Mochida Memorial Foundation for Medical and Pharmaceutical Research
- Foundation for Promotion of Cancer Research in Japan
- Suzuken Memorial Foundation
- Shimabara Science Promotion Foundation
- Astellas Foundation for Research on Metabolic Disorders
Ask authors/readers for more resources
5-Fluorouracil (5-FU) is widely used in the treatment of various types of solid cancer. Our study showed that ribosomal protein L11 (RPL11) was a crucial factor affecting sensitivity of gastric cancer to 5-FU, implying that RPL11 expression is a potential biomarker for predicting 5-FU sensitivity. Kaplan-Meier survival analysis indicated that high RPL11 expression in gastric cancer patients treated with 5-FU was significantly associated with good prognosis. It was therefore investigated whether RPL11 affected the sensitivity of gastric cancer against 5-FU using four human gastric cancer cell lines, MKN45 (wild-type TP53 gene), NUGC4 (wild-type), MKN7 (mutated), and KE39 cells (mutated). In vitro assays demonstrated that RPL11 knockdown in gastric cancer cell lines carrying the TP53 wild-type gene attenuated 5-FU-induced cell growth suppression and activation of the P53 pathway, but not in cells carrying mutated TP53, suggesting that 5-FU suppresses tumor progression via RPL11-mediated activation of the P53 pathway in gastric cancer. The present study provides a potential therapeutic strategy for improving 5-FU resistance in gastric cancer by elevating RPL11 expression.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available