Article
Biochemistry & Molecular Biology
Haotian Wei, Li Chen, Qing Li, Xinjun Liang, Kun Wang, Ying Zhang, Yueqiang Li, Yanyan Liu, Gang Xu
Summary: This study examines the role of CD137 in angiogenesis and progression of renal diseases, particularly in IgA nephropathy. The results suggest that CD137 interacts with macrophages and lymphatic endothelial cells to promote lymphangiogenesis in the kidney, which further drives fibrogenic responses.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Oncology
Maureen Van de Velde, Marie Ebroin, Tania Durre, Marc Joiret, Lionel Gillot, Silvia Blacher, Liesbet Geris, Frederic Kridelka, Agnes Noel
Summary: This study revealed a new mechanism by which tumor exposed-LEC promote tumor cell proliferation, showcasing their role in enhancing cancer cell invasion and proliferation in vivo. The analysis of teLEC secretome identified IL6 as a key molecule modulated in teLEC, with neutralization of IL6 abolishing the mitogenic effects mediated by teLEC in vivo. The findings suggest a novel function for teLEC beyond their traditional role in lymphatic vessel formation, emphasizing their potential as key stromal determinants in future studies.
Article
Biochemistry & Molecular Biology
Li Zhang, Linyu Zhu, Xiaohan Yao, Xiaohan Lou, Jiajia Wan, Xixi Duan, Longze Pan, Anqi Li, Zhuoyu Gu, Ming Wang, Fazhan Wang, Zhihai Qin
Summary: Chemotherapeutic drug paclitaxel (PTX) inhibits tumor growth at the injection site but enhances lymphatic metastasis of melanoma cells by upregulating C-C chemokine receptor type 7 (CCR7) expression and activating fNK and p38 signaling pathways.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Cell Biology
Xiaopeng Chen, Bin Cheng, Dafei Dai, Yuhai Wu, Zhiwen Feng, Chaogang Tong, Xiangming Wang, Jun Zhao
Summary: High expression of HPSE promotes HCC metastasis by mediating the necroptosis of MVECs and activating the HPSE/SDC-1/TNF-α axis and p38 MAPK pathway.
CELL DEATH DISCOVERY
(2021)
Article
Multidisciplinary Sciences
Meng Wang, Yue Li, Yunyun Xiao, Muwen Yang, Jinxin Chen, Yunting Jian, Xin Chen, Dongni Shi, Xiangfu Chen, Ying Ouyang, Lingzhi Kong, Xinjian Huang, Jiewen Bai, Chuyong Lin, Libing Song
Summary: This study demonstrates that nicotine promotes lymphatic metastasis by preventing mRNA decay of VEGF-C mRNA through downregulating OTUD3 and ZFP36. The interaction between OTUD3 and ZFP36 stabilizes ZFP36 and inhibits VEGF-C mRNA decay, highlighting their crucial role in nicotine addiction-induced lymphatic metastasis in esophageal cancer.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Chenfei Zhou, Yanmei Zhang, Ruiming Yan, Lei Huang, Andrew L. Mellor, Yang Yang, Xiaojing Chen, Wenfei Wei, Xiangguang Wu, Lan Yu, Luojiao Liang, Dan Zhang, Sha Wu, Wei Wang
Summary: The high levels of miR-142-5p in advanced CSCC positively correlate with IDO expression in tumor-associated lymphatic vessels. The miR-142-5p is transferred by CSCC-secreted exosomes into LECs to induce IDO expression via ARID2-DNMT1-IFN-gamma signaling, leading to CD8(+)T cell exhaustion. This study suggests that LECs play a crucial role as immune checkpoints in the TME and may be a potential new target for CSCC diagnosis and treatment.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Oncology
Ofri Doppelt-Flikshtain, Amin Younis, Tal Tamari, Ofir Ginesin, Talia Shentzer-Kutiel, David Nikomarov, Gil Bar-Sela, Benjamin R. Coyac, Yehuda G. Assaraf, Hadar Zigdon-Giladi
Summary: Despite progress in the treatment of various cancers, the prognosis for patients with metastatic osteosarcoma remains poor. This study demonstrated that endothelial progenitor cells (EPCs) play a role in promoting osteosarcoma progression and aggressiveness through non-physical interactions, and revealed a potential underlying molecular mechanism. These findings suggest the possibility of developing EPCs-targeted therapies to inhibit osteosarcoma metastasis and improve treatment efficacy.
Article
Biochemistry & Molecular Biology
Zitong Zhao, Heyang Xu, Yuan Wei, Li Sun, Yongmei Song
Summary: This study investigates the role of DUBs in aberrant autophagy in ovarian cancer and identifies that the deubiquitinase PSMD14 negatively regulates autophagy through the LRPPRC/Beclin1-Bcl-2/SQSTM1 signaling pathway.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Biotechnology & Applied Microbiology
Xiaolong Li, Yujie Wen, Liyuan Sheng, Rui Guo, Yanli Zhang, Longquan Shao
Summary: The study showed that lcariin (ICA) can promote the angiogenesis capacity of vascular endothelial cells by activating autophagy. These findings provide a theoretical basis for understanding the effect of ICA on vascular endothelial cells.
Article
Oncology
Liwei Wang, Jiazhong Shi, Sha Liu, Yaqin Huang, Hua Ding, Baixiong Zhao, Yuting Liu, Wuxing Wang, Jin Yang, Zhiwen Chen
Summary: The study found that RAC3 is highly expressed in bladder cancer and is associated with poor prognosis. Silencing RAC3 can exert anti-tumor effects by enhancing PI3K/AKT/mTOR-mediated autophagy. Simultaneously targeting RAC3 and autophagy may be a potential therapeutic strategy for inhibiting bladder cancer progression and prolonging survival.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Rui Li, Yanyu Hao, Qiuhan Wang, Yuan Meng, Kunhe Wu, Chaoqun Liu, Lijun Xu, Ziguang Liu, Liang Zhao
Summary: This study identified ECHS1 as a key player in promoting CRC cell proliferation, migration, and invasion by altering Cer metabolism and releasing ROS. Inhibition of ECHS1 reversed the survival and drug resistance of CRC cells, offering potential targets for future therapies.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Chih-Ming Su, Tung-Wei Hsu, Hsin-An Chen, Wan-Yu Wang, Chih-Yang Huang, Chih-Chiang Hung, Ming-Hsin Yeh, Yen-Hao Su, Ming-Te Huang, Po-Hsiang Liao
Summary: Metastasis in breast cancer is the main cause of death in clinical patients. Lower levels of Dicer protein in highly invasive breast cancer cells are usually associated with poor clinical outcomes. Our study reveals the regulatory mechanism of Dicer protein degradation through a process called chaperone-mediated autophagy (CMA), which promotes cancer metastasis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Oncology
Wen-Fei Wei, Hui-Ling Zhou, Pei-Yu Chen, Xiao-Lan Huang, Long Huang, Luo-Jiao Liang, Chu-Hong Guo, Chen-Fei Zhou, Lan Yu, Liang-Sheng Fan, Wei Wang
Summary: In this study, it was found that cancer-associated fibroblast (CAF)-derived PAI-1 promotes the endothelial-mesenchymal transition (EndoMT) of lymphatic endothelial cells (LECs) in cervical squamous cell carcinoma (CSCC). LECs undergoing EndoMT can stimulate tumor neolymphangiogenesis and promote lymphatic metastasis in CSCC. PAI-1 activates the AKT/ERK1/2 pathways by directly interacting with low-density lipoprotein receptor-related protein (LRP1), leading to elevated EndoMT activity in LECs. PAI-1 could be a potential prognostic biomarker and therapeutic target for CSCC metastasis.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Oncology
Sergio Ortiz-Espinosa, Xabier Morales, Yaiza Senent, Diego Alignani, Beatriz Tavira, Irati Macaya, Borja Ruiz, Haritz Moreno, Ana Remirez, Cristina Sainz, Alejandro Rodriguez-Pena, Alvaro Oyarbide, Mikel Ariz, Maria P. Andueza, Karmele Valencia, Alvaro Teijeira, Kai Hoehlig, Axel Vater, Barbara Rolfe, Trent M. Woodruff, Jose Maria Lopez-Picazo, Silvestre Vicent, Grazyna Kochan, David Escors, Ignacio Gil-Bazo, Jose Luis Perez-Gracia, Luis M. Montuenga, John D. Lambris, Carlos Ortiz de Solorzano, Fernando Lecanda, Daniel Ajona, Ruben Pio
Summary: This study reveals that C5a can enhance the capacity of PMN-MDSCs to promote tumor growth and metastasis by inducing the formation of NETs. The formation of NETs is dependent on the production of HMGB1 by cancer cells. Inhibiting C5a, C5aR1, or NETosis can reduce the number of circulating tumor cells and the metastatic burden in a mouse lung metastasis model. The translational relevance of these findings is supported by the stimulation of migration and NETosis in PMN-MDSCs obtained from lung cancer patients.
Article
Immunology
Chunchen Wu, Luzhi Zeng, Wenfu Yi, Yuanjiu Miao, Yihan Liu, Qiming Wang, Shi Liu, Guoping Peng, Zhenhua Zheng, Jianbo Xia
Summary: This study demonstrates that echovirus infection can induce autophagy, which promotes viral replication and VP1 expression in host cells. The mechanism involves the regulation of the mTOR/ULK1 signaling pathway, providing insight into the potential role of autophagy in echovirus infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Emilia Gore, Thibaut Duparc, Annelise Genoux, Bertrand Perret, Souad Najib, Laurent O. Martinez
Summary: IF1 is an endogenous inhibitor of ATP synthase, involved in mitochondrial functions, energy metabolism, redox balance, and cell fate. Despite recent paradigm shifts regarding IF1 functions, its study in entire organisms and different organs remains limited. The investigation of IF1 at multiple levels and in various pathophysiological conditions may help uncover therapeutic strategies in human pathology.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Cell Biology
Carole Nicco, Marine Thomas, Julie Guillermet, Maryline Havard, Fanny Laurent-Tchenio, Ludivine Doridot, Francois Dautry, Frederic Batteux, Thierry Tchenio
Summary: Self-sustained quiescence (SSQ) is a stable but reversible non-proliferative cellular state that limits the cloning of cultured cancer cells. It has been found that low cell density and the use of drugs can induce cells into SSQ state. In SSQ state, the tumor growth rate of pancreatic adenocarcinoma cells slows down, but eventually transitions to a rapid growth phase. Identifying new agents targeting cancer cells in SSQ may improve patient survival.
Article
Endocrinology & Metabolism
Julie Pires Da Silva, Matthieu Wargny, Jeremy Raffin, Mikael Croyal, Thibaut Duparc, Guillaume Combes, Annelise Genoux, Bertrand Perret, Bruno Vellas, Sophie Guyonnet, Claire Thalamas, Dominique Langin, Cedric Moro, Nathalie Viguerie, Yves Rolland, Philipe de Souto Barreto, Bertrand Cariou, Laurent O. Martinez
Summary: This study analyzed the association between plasma ATPase inhibitory factor 1 (IF1) levels and markers of glucose homeostasis as well as the development of new-onset diabetes (NOD) in individuals with prediabetes. The results showed that IF1 levels were negatively correlated with clinical variables associated with obesity and insulin resistance, and positively correlated with markers of cardiometabolic health. Furthermore, the study found a significant association between IF1 levels and the risk of type 2 diabetes mellitus (T2DM).
DIABETES & METABOLISM
(2023)
Article
Biology
Agnes Ribes, Cedric Garcia, Marie-Pierre Gratacap, Evi Kostenis, Laurent O. Martinez, Bernard Payrastre, Jean-Michel Senard, Celine Gales, Veronique Pons
Summary: This study investigated the pharmacology of P2Y(1)-R signaling in various cells and platelets and found that P2Y(1)-R is constitutively active in physiological conditions. The study also identified P2Y(1)-R antagonists that can inhibit this constitutive activation. These findings suggest that targeting P2Y(1)-R constitutive activation could be a promising strategy for antiplatelet therapy.
Review
Geriatrics & Gerontology
Jaqueline Aragoni da Silva, Yves Rolland, Laurent O. Martinez, Philipe de Souto Barreto
Summary: Mitochondrial dysfunction may contribute to age-related decline in intrinsic capacity across multiple domains. However, the evidence regarding the associations between mitochondrial function and intrinsic capacity domains is still limited. Most studies have been cross-sectional with small sample sizes, primarily investigating skeletal muscle and peripheral blood mononuclear cells. The available evidence suggests that mitochondrial function, particularly mitochondrial DNA copy number, is associated with all intrinsic capacity domains, but more robust evidence is found for locomotion than hearing. Further research is needed to confirm the role of mitochondria in maintaining optimal function and promoting healthy aging.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)
Review
Oncology
Benoit Thibault, Fernanda Ramos-Delgado, Julie Guillermet-Guibert
Summary: Phosphatidylinositol-3-kinase (PI3K) enzymes play a key role in intracellular signaling and vesicular trafficking pathways by producing signaling phosphoinositides at plasma and intracellular membranes. There are eight isoforms of PI3K divided into three classes, which have various functions in physiology and are often deregulated in cancer. Recent evidence has shown that PI3K isoforms of class I, II, and III play important roles in tumor biology and that PI3K inhibitors have anti-tumoral action in preclinical cancer models. Understanding the functions of class II and III isoforms can lead to the development of combination treatment strategies targeting multiple PI3K isoforms simultaneously.
Article
Geriatrics & Gerontology
Leonardo Bencivenga, Mathilde Strumia, Yves Rolland, Laurent Martinez, Philippe Cestac, Sophie Guyonnet, Sandrine Andrieu, Angelo Parini, Alexandre Lucas, Bruno Vellas, Philipe De Souto Barreto, Laure Rouch
Summary: This study aimed to determine the association between plasma levels of the stress-related mitokine growth/differentiation factor 15 (GDF-15), inflammatory biomarkers interleukin 6 (IL-6) and tumor necrosis factor receptor 1 (TNFR-1), and visit-to-visit blood pressure variability (BPV) in community-dwelling older adults. The results showed that higher levels of GDF-15 were significantly associated with increased systolic blood pressure variability (SBPV), but not diastolic blood pressure variability (DBPV).
Article
Geriatrics & Gerontology
Jaqueline Aragoni da Silva, Laurent O. Martinez, Yves Rolland, Souad Najib, Mikael Croyal, Bertrand Perret, Nabila Jabrane-Ferrat, Hicham El Costa, Sophie Guyonnet, Bruno Vellas, Philipe de Souto Barreto, MAPT DSA grp
Summary: This study investigated the association between plasma levels of ATPase inhibitory factor 1 (IF1) and intrinsic capacity (IC) in community-dwelling older adults. The findings showed that plasma IF1 levels were associated with IC composite scores, with both low-intermediate and high-intermediate levels being linked to higher scores. This study is the first to demonstrate the relationship between circulating IF1 levels and IC in older adults, but further research is needed to understand the underlying mechanisms.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)
Article
Cell Biology
Jules Merian, Lamia Ghezali, Charlotte Trenteseaux, Thibaut Duparc, Diane Beuzelin, Vanessa Bouguetoch, Guillaume Combes, Nabil Sioufi, Laurent O. Martinez, Souad Najib
Summary: Intermittent fasting interventions can improve insulin sensitivity, lipid metabolism, and prevent atherosclerosis. However, the effects may vary based on sex and dietary composition.
Article
Geriatrics & Gerontology
Wan-Hsuan Lu, Emmanuel Gonzalez-Bautista, Sophie Guyonnet, Laurent O. Martinez, Alexandre Lucas, Angelo Parini, Yves Rolland, Bruno Vellas, Philipe de Souto Barreto, MAPT DSA Grp
Summary: This study investigated the association between vitality and other intrinsic capacity domains and functional difficulties using three operational definitions of vitality. The results showed that vitality had indirect effects on instrumental activities of daily living (IADL), primarily through cognitive, locomotor, and psychological domains. Biomarkers might be a more suitable measurement for the early detection of locomotion decline.
Article
Geriatrics & Gerontology
Laetitia Lengele, Yves Rolland, Laurent O. Martinez, Sophie Guyonnet, Angelo Parini, Alexandre Lucas, Bruno Vellas, Philipe de Souto Barreto, MAPT DSA Grp
Summary: Plasma levels of GDF-15 are associated with key malnutrition criteria.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)
Review
Biology
Celine Schmitter, Mickael Di-Luoffo, Julie Guillermet-Guibert
Summary: In living organisms, cells can sense mechanical forces and respond to them through a process called mechanotransduction. Recent studies on human cells have shown that compressive forces can selectively regulate cell behavior, both in compressed and neighboring cells. This review summarizes the current knowledge of compression-induced cell signaling pathways and their effects in both physiological and pathological conditions, such as solid cancers.
LIFE SCIENCE ALLIANCE
(2023)
Article
Geriatrics & Gerontology
Wan-Hsuan Lu, Sophie Guyonnet, Laurent O. Martinez, Alexandre Lucas, Angelo Parini, Bruno Vellas, Philipe de Souto Barreto
Summary: This study investigated the association between longitudinal intrinsic capacity trajectories and plasma biomarkers of chronic inflammation and mitochondrial dysfunction in older adults. The findings showed that elevated levels of IL-6, TNFR-1, and GDF-15 were associated with a higher risk of multi-impaired intrinsic capacity trajectories.
Article
Biochemical Research Methods
Zacchari Ben Meriem, Tiphaine Mateo, Julien Faccini, Celine Denais, Romane Dusfour-Castan, Catherine Guynet, Tatiana Merle, Magali Suzanne, Mickael Di-Luoffo, Julie Guillermet-Guibert, Baptiste Alric, Sylvain Landiech, Laurent Malaquin, Fabien Mesnilgrente, Adrian Laborde, Laurent Mazenq, Remi Courson, Morgan Delarue
Summary: Conventional culture conditions lack dynamic control over the microenvironment, hindering the study of tissues and organisms. In this study, a microfluidic device with sliding elements is presented, allowing for the control of both chemical and mechanical compressive forces. By confining micro-tissues, the impact of growth-induced pressure and macromolecular crowding was studied, shedding light on an understudied type of mechanical stress.
Article
Biochemical Research Methods
Zacchari Ben Meriem, Tiphaine Mateo, Julien Faccini, Celine Denais, Romane Dusfour-Castan, Catherine Guynet, Tatiana Merle, Magali Suzanne, Mickael Di-Luoffo, Julie Guillermet-Guibert, Baptiste Alric, Sylvain Landiech, Laurent Malaquin, Fabien Mesnilgrente, Adrian Laborde, Laurent Mazenq, Remi Courson, Morgan Delarue
Summary: Conventional culture conditions lack dynamic chemical and mechanical control over the microenvironment, making them insufficient for studying tissues, organisms, or 3D multicellular assemblies. This study presents a microfluidic device that allows control of both chemical and mechanical compressive forces, enabling the study of biological samples under specific conditions and the impact of mechanical compression. The device utilizes sliding elements to create reconfigurable closed culture chambers for the study of whole organisms or model micro-tissues.