Journal
BIOSENSORS & BIOELECTRONICS
Volume 69, Issue -, Pages 230-234Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.02.041
Keywords
Electrochemical biosensor; Lead ion; DNA self-assembly; Hemin/G-quadruplex; Polymerization; Polyaniline
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Funding
- National Natural Science Foundation of China, China [41176079, 21475025]
- National Science Foundation of Fujian Province [2014107001]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1116]
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Lead ion (Pb2+, a heavy metal ion existed every aspect of life) is one of the most important hazards for environment and human health. Herein we design an in-situ amplified electronic monitoring system for sensitive determination of Pb2+ on Pb2+-specific DNAzyme-modified sensing interface. The assay consists of target-triggered cleavage of Pb2+-specific DNAzyme, initiator strand-induced DNA hybridization chain reaction, formation of hemin/G-quadruplex -based DNAzyme and its automatically catalyzed polymerization of aniline monomer along the double-stranded DNA. Experimental results indicated that the catalytic currents of the as-produced polyaniline were linearly dependent on target Pb2+ concentrations from 0.05 to 50 nM with a detection limit (LOD) of 32 pM. Such a synergistic effect of hybridization chain reaction with DNAzyme-catalyzed polymerization provided a universal platform for sensitive screening of target Pb2+, thereby holding great promise for application in practice. (C) 2015 Elsevier B.V. All rights reserved.
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