Journal
CANCER LETTERS
Volume 378, Issue 2, Pages 142-149Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2016.05.020
Keywords
Cell-cell interaction; Migration; Time-lapse imaging
Categories
Funding
- Korean Government [NRF-MRC 2008-0062275, KHIDI-HI15C0778]
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The antitumor activity of cytokine-induced killer (CIK) cells can be increased by co-culturing them with tumor lysate-pulsed dendritic cells (tDCs); this phenomenon has been studied mainly at the populadon level. Using time-lapse imaging, we examined how CIK cells gather information from tDCs at the single-cell level. tDCs highly expressed CCL5, which bound CCR5 expressed on CIK cells. tDCs strongly induced migration of Ccr54(+/+) CIK cells, but not that of Ccr5(-/-) CIK cells or Ccr5(+/+) CIK cells treated with the CCR5 antagonist Maraviroc. Individual tDCs contacted Ccr(+/+) CIK cells more frequently and lengthily than with Ccr5(-/-) CIK cells. Consequently, tDCs increased the antitumor activity of Ccr5(+/+) CIK cells in vitro and in vivo, but did not increase that of Ccr5(-/-) CIK cells. Taken together, our data provide insight into the mechanism of CIK cell activation by tDCs at the single-cell level. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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