Article
Immunology
Xiyuan Bai, Deepshikha Verma, Cindy Garcia, Ariel Musheyev, Kevin Kim, Lorelenn Fornis, David E. Griffith, Li Li, Nicholas Whittel, Jacob Gadwa, Tamara Ohanjanyan, Matthew J. Eggleston, Manuel Galvan, Brian M. Freed, Diane Ordway, Edward D. Chan
Summary: Cigarette smoke exposure can impair the immune response against Mycobacterium tuberculosis (MTB) infection. In this study, it was found that cigarette smoke-exposed T regulatory cells (Tregs) can exacerbate MTB infection by impairing macrophage function and promoting immunosuppression. This was demonstrated through co-culture experiments and mouse models. These findings suggest that cigarette smoke not only directly affects immune cells, but also indirectly promotes TB infection through the modulation of Tregs.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Mohamed B. Ezzelarab, Hong Zhang, Kazuki Sasaki, Lien Lu, Alan F. Zahorchak, Dirk J. van der Windt, Helong Dai, Angelica Perez-Gutierrez, Jay K. Bhama, Angus W. Thomson
Summary: The study investigated the relationship between infused Treg and different immunosuppressive regimens, revealing a lack of therapeutic efficacy in lymphodepleted heart graft recipients, possibly due to loss of regulatory features and proliferative capacity shortly after infusion.
Article
Respiratory System
Ei Miyamoto, Akihiro Takahagi, Akihiro Ohsumi, Tereza Martinu, David Hwang, Kristen M. Boonstra, Betty Joe, Juan Mauricio Umana, Ke F. Bei, Daniel Vosoughi, Mingyao Liu, Marcelo Cypel, Shaf Keshavjee, Stephen C. Juvet
Summary: Pre-transplant administration of recipient-derived Tregs through EVLP can maintain their immunomodulatory function within the donor lungs.
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Immunology
Elisabet Van Loon, Baptiste Lamarthee, Thomas Barba, Sandra Claes, Maarten Coemans, Henriette de Loor, Marie-Paule Emonds, Priyanka Koshy, Dirk Kuypers, Paul Proost, Aleksandar Senev, Ben Sprangers, Claire Tinel, Olivier Thaunat, Amaryllis H. Van Craenenbroeck, Dominique Schols, Maarten Naesens
Summary: Through multiplex assay, we found that kidney transplant patients with donor-specific anti-human leukocyte antigen antibodies and histological rejection have increased levels of pro-inflammatory cytokines in blood. Even in patients without histological rejection, elevated pro-inflammatory cytokine levels can be observed. These findings challenge the concept of histology as the gold standard for identifying ongoing allo-immune activation after transplantation.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Min Hu, Natasha M. Rogers, Jennifer Li, Geoff Y. Zhang, Yuan Min Wang, Karli Shaw, Philip J. O'Connell, Stephen Alexander
Summary: Tregs play a crucial role in kidney transplantation by limiting immune activation and potentially reducing the need for immunosuppression. Studies have shown their importance in improving allo-specific Treg function in both animal and human models.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Sybille Landwehr-Kenzel, Leonie Mueller-Jensen, Joern-Sven Kuehl, Mohamed Abou-el-Enein, Henrike Hoffmann, Sandra Muench, Daniel Kaiser, Andy Roemhild, Horst von Bernuth, Mirjam Voeller, Michael Schmueck-Henneresse, Bernd Gruhn, Ulrik Stervbo, Nina Babel, Hans-Dieter Volk, Petra Reinke
Summary: This study explored the use of adoptive transfer of ex vivo expanded regulatory T cells (Tregs) for the treatment of chronic graft-versus-host disease (GvHD). The results showed that Tregs therapy was well tolerated and capable of modulating established adverse T cell mediated allo-response in patients.
Article
Immunology
Sudipta Tripathi, Paloma L. Martin-Moreno, George Kavalam, Brittany L. Schreiber, Ana Maria Waaga-Gasser, Anil Chandraker
Summary: This study demonstrates the significant role of the adenosinergic pathway in the suppressive/regulatory effects of antigen specific regulatory T cells. Furthermore, it suggests the possibility of combining infusion of Tregs with adenosine receptor agonists or increasing CD39 expression to enhance the regulatory response to allografts.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Surgery
Matteo Di Nardo, Lorenzo Del Sorbo, Andrew Sage, Jin Ma, Mingyao Liu, Jonathan C. Yeung, Jerome Valero, Rasheed Ghany, Marcelo Cypel, Shaf Keshavjee
Summary: This study developed a mathematical model (EXPIRE model) using ex vivo lung perfusion technique to predict lung acceptance, which was successfully validated in a separate cohort. The model may help standardize lung assessment and improve transplant rates in centers using the Toronto EVLP technique.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Engineering, Biomedical
Turaab Khan, Jeremy Kwarcinski, Philip Boughton, Peter Yoon, Ahmer Hameed, Animesh Singla, Tony Pang, Henry Pleass
Summary: This study primarily focused on determining the effect of a novel thermally insulating jacket on the thermal profile of the human kidney and quantifying the reduction in thermal energy experienced using this device. The results showed that the kidneys with the thermally insulating jacket reached the metabolic threshold temperature later and stayed below the threshold for a longer period compared to the control group.
Article
Surgery
Paul N. Harden, David S. Game, Birgit Sawitzki, Jeroen B. van der Net, Joanna Hester, Andrew Bushell, Fadi Issa, Matthew O. Brook, Alaa Alzhrani, Stephan Schlickeiser, Cristiano Scotta, William Petchey, Mathias Streitz, Gilles Blancho, Quizhi Tang, James Markmann, Robert I. Lechler, Ian S. D. Roberts, Peter J. Friend, Rachel Hilton, Edward K. Geissler, Kathryn J. Wood, Giovanna Lombardi
Summary: Using autologous polyclonal regulatory T cell therapy in kidney transplantation can reduce immunosuppression dosage, leading to improved long-term clinical outcomes with potentially lower rejection rates compared to standard immunosuppression.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Cell Biology
Aizhou Wang, Rafaela V. P. Ribeiro, Aadil Ali, Edson Brambate, Etienne Abdelnour-Berchtold, Vinicius Michaelsen, Yu Zhang, Peter Rahfeld, Haisle Moon, Hemant Gokhale, Anajara Gazzalle, Prodipto Pal, Mingyao Liu, Thomas K. Waddell, Christine Cserti-Gazdewich, Kathryn Tinckam, Jayachandran N. Kizhakkedathu, Lori West, Shaf Keshavjee, Stephen G. Withers, Marcelo Cypel
Summary: The enzymes FpGalNAc deacetylase and FpGalactosaminidase can effectively remove A antigen from donor lungs, potentially expanding ABO-incompatible lung transplantation and improving fairness of organ allocation. The removal of A antigen was achieved through ex vivo lung perfusion treatment and showed positive results in reducing antibody-mediated rejection.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Immunology
Nicholas G. Larkins, Lloyd D'Orsogna, Anne Taverniti, Ankit Sharma, Aron Chakera, Doris Chan, Anoushka Krishnan, Germaine Wong, Wai H. Lim
Summary: High resolution HLA typing is crucial for eplet compatibility and specificity of DSA. NGS and LD typing show high concordance but significant differences. Inaccurate DSA assignment from LD typing may inappropriately exclude candidates.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Medicine, General & Internal
Ruta Zulpaite, Povilas Miknevicius, Bettina Leber, Kestutis Strupas, Philipp Stiegler, Peter Schemmer
Summary: Kidney transplantation is the gold standard treatment for patients with end-stage kidney disease, but the quality of donor organs poses limitations. Ex-vivo machine perfusion technology has great potential in maintaining kidney viability and optimizing organ treatment, serving as an important component of novel techniques in transplant surgery.
FRONTIERS IN MEDICINE
(2021)
Article
Oncology
Marie Tourret, Nana Talvard-Balland, Marion Lambert, Ghada Ben Youssef, Mathieu F. Chevalier, Armelle Bohineust, Thomas Yvorra, Florence Morin, Saba Azarnoush, Olivier Lantz, Jean-Hugues Dalle, Sophie Caillat-Zucman
Summary: MAIT cells are semi-invariant T cells that recognize microbial antigens presented by MR1. They are predominantly found in the liver and barrier tissues, and are potent effectors of antimicrobial defense. Although they have potential therapeutic applications, it remains to be demonstrated whether they lack alloreactive potential.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Immunology
Matthias Niemann, Yara Strehler, Nils Lachmann, Fabian Halleck, Klemens Budde, Gideon Hoenger, Stefan Schaub, Benedict M. Matern, Eric Spierings
Summary: Development of donor-specific human leukocyte antigen (HLA) antibodies (DSA) is a major risk factor for graft loss following organ transplantation. This study used computational deep learning to define the surface residues of HLA Class I proteins and found a correlation between the number of solvent-accessible interlocus amino acid mismatches (Snowflake) and the incidence of DSA.
FRONTIERS IN IMMUNOLOGY
(2022)