Article
Biochemistry & Molecular Biology
Fanlu Wang, Lena Marie Saure, Fabian Schuett, Felix Lorich, Florian Rasch, Ali Shaygan Nia, Xinliang Feng, Andreas Seekamp, Tim Klueter, Hendrik Naujokat, Rainer Adelung, Sabine Fuchs
Summary: Graphene oxide shows promising potential in bone tissue engineering, but its molecular biological effects in clinically applied materials are still limited. This study compares the effects of graphene oxide framework structures (F-GO) and reduced graphene oxide-based framework structures (F-rGO) on bone vascularization processes. Non-reduced graphene oxide in the form of F-GO is favored for bone regeneration applications, and graphene oxide coating on a clinically used bone graft improves cell adhesion and vascularization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Laura Elomaa, Marcus Lindner, Ruth Leben, Raluca Niesner, Marie Weinhart
Summary: This study combines cell sheet engineering, 3D printing, and cellular self-organization to create a vascular-like 3D network. The results demonstrate that a cell sheet-covered vessel mimic and self-assembling vascular endothelial cells can spontaneously form a dense vascular network in a collagen gel under specific culture conditions.
Article
Dentistry, Oral Surgery & Medicine
Yanming Weng, Zhifa Wang, Jianwei Sun, Leng Han, Xiao Li, Buling Wu, Qingshan Dong, Yanpu Liu
Summary: The study successfully generated vascularized bone tissue by combining a coral scaffold with BMSCs sheet, and demonstrated that inserting axial vessels significantly promotes angiogenesis and osteogenesis. This method shows great potential for clinical applications in repairing large bone defects.
JOURNAL OF STOMATOLOGY ORAL AND MAXILLOFACIAL SURGERY
(2021)
Article
Engineering, Biomedical
Zixuan Lin, Xiurui Zhang, Madalyn R. Fritch, Zhong Li, Biao Kuang, Peter G. Alexander, Tingjun Hao, Guorui Cao, Susheng Tan, Katherine K. Bruce, Hang Lin
Summary: Most in vitro engineered bone tissues lack viable blood vessel systems, which limits their ability to repair large bone defects. This study successfully created pre-vascularized bone-like tissue by simulating human bone development, resulting in the formation of functional blood vessels and bone tissue, demonstrating its potential for bone defect repair.
Review
Nanoscience & Nanotechnology
Qi You, Minxun Lu, Zhuangzhuang Li, Yong Zhou, Chongqi Tu
Summary: Congenital or acquired bone defects present a challenge to the global healthcare system, and novel cell sheet technology shows promise for promoting bone regeneration. This review introduces and explores the application of cell sheet technology in bone regeneration, highlights the current state of research, and offers perspectives on translating this knowledge to clinical practice.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Hidekazu Sekine, Teruo Okano
Summary: One of the key challenges in regenerative medicine is to introduce vascular networks into bioengineered tissues. Co-culturing transplant tissue with vascular cells before transplantation has been explored as a way to promote capillary angiogenesis and construct a continuous circulatory structure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Veterinary Sciences
Zihang Ma, Xiaoying Guo, Jun Zhang, Qifeng Jiang, Wuying Liang, Wenxin Meng, Shuaijiang Chen, Yufan Zhu, Cundong Ye, Kun Jia
Summary: This study aimed to evaluate the therapeutic effect of combining canine bone marrow mesenchymal stem cells (cBMSCs) with gelatin-nano-hydroxyapatite (Gel-nHAP) on bone defect diseases in dogs. The results showed that Gel-nHAP supported the attachment of cBMSCs and exhibited good biocompatibility. In animal experiments, significant cortical bone growth was observed in the Gel-nHAP group at week 8 and in the cBMSCs-Gel-nHAP group at week 4.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Nanoscience & Nanotechnology
Chentao Shen, Jian Liu, Qiyi Lu, Guihua Wang, Zhenxing Wang, Lu Liu
Summary: In this study, the physicochemical and mechanical properties as well as biocompatibility of GO-Gel scaffolds were investigated. A pre-vascularized V-GO-Gel chamber model was successfully built, which showed promising therapeutic potential in the repair of intestinal wall defects.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Nicholas G. Schott, Huy Vu, Jan P. Stegemann
Summary: This study used a modular approach to encapsulate adult human mesenchymal stromal cells (MSC) in biomaterial carriers for bone repair. The results demonstrated that preculturing enhances the differentiation of the cells, and that osteogenic microtissues still maintain their bone-forming activity even when cultured in a vasculogenic medium, while vasculogenic priming induces the development of a primitive vessel network. This modular approach harnesses the dual differentiation potential of MSC to support bone and blood vessel formation.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
WonJin Kim, GeunHyung Kim
Summary: A new cell-printing process is introduced in this study, which allows the fabrication of cell-spheroids and cell-loaded constructs together without the need to prepare cell-spheroids in advance. The diameter and location of spheroids can be manipulated by controlling printing parameters. Hybrid cell constructs fabricated using this method exhibit strong angiogenic and osteogenic activities.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Engineering, Biomedical
Maximilian G. Burger, Andrea Grosso, Priscilla S. Briquez, Gordian M. E. Born, Alexander Lunger, Flavio Schrenk, Atanas Todorov, Veronica Sacchi, Jeffrey A. Hubbell, Dirk J. Schaefer, Andrea Banfi, Nunzia Di Maggio
Summary: In regenerative medicine, rapid vascularization of clinical-size bone grafts is a challenge that has not been solved. This study investigates the distribution of VEGF protein on matrices to achieve efficient coupling of angiogenesis and bone formation, providing a clinically applicable strategy for engineering vascularized bone.
ACTA BIOMATERIALIA
(2022)
Article
Materials Science, Biomaterials
Zhenhua Zhao, Mang Wang, Fei Shao, Ge Liu, Junlei Li, Xiaowei Wei, Xiuzhi Zhang, Jiahui Yang, Fang Cao, Qiushi Wang, Huanan Wang, Dewei Zhao
Summary: The study developed an integrated three-dimensional scaffold material using pTa and GNPs hydrogel, seeded with BMSCs-derived ECs to promote the formation of a stable capillary-like network. The scaffold exhibited superior biocompatibility and biomechanical properties, showing potential as a vascular tissue engineering material.
REGENERATIVE BIOMATERIALS
(2021)
Article
Cell Biology
Ulrike Rottensteiner-Brandl, Ulf Bertram, Lara F. Lingens, Katrin Koehn, Luitpold Distel, Tobias Fey, Carolin Koerner, Raymund E. Horch, Andreas Arkudas
Summary: In this study, a critical-sized femoral defect model was combined with an ionizing radiation protocol in rats to support bone healing using a combination of SiHA, MSCs and BMP-2. The implanted osteogenic MSCs survived in vivo for 18 weeks, while irradiation led to impaired bone healing.
Article
Biotechnology & Applied Microbiology
Ning Zeng, Youbai Chen, Yewen Wu, Mengqing Zang, Rene D. Largo, Edward I. Chang, Mark V. Schaverien, Peirong Yu, Qixu Zhang
Summary: Researchers used partial decellularization to preserve tracheal cartilage as a scaffold. By combining bioengineering and cryopreservation techniques, they successfully fabricated a neo-trachea that can bear neck movement, prevent fibrosis obliteration, maintain airway patency, and achieve neovascularization. This approach provides a promising strategy for tracheal tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Farshid Bastami, Seyedeh-Mina Safavi, Sina Seifi, Nasser Nadjmi, Arash Khojasteh
Summary: A 3D-printed biodegradable hydrogel has been developed as a scaffold to enhance cell adhesion, proliferation, and osteogenic differentiation. It shows promising potential for bone regeneration, surpassing the conventional clinical approach.
MACROMOLECULAR BIOSCIENCE
(2023)
