Review
Medicine, Research & Experimental
Du Xiang, Yang Liu, Encheng Zhou, Yanfeng Wang
Summary: This article discusses the gradual decrease in ovarian reserve with age, the related diseases that may occur after the loss of estrogen protection postmenopausally, as well as the controversy surrounding PHRT and AO technology. The article also compares the advantages and disadvantages of different polymer biomaterials for use in follicle IVC, providing a theoretical basis for the development of mature AO technology.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Medicine, General & Internal
Xia Hao, Amandine Anastacio, Kenny A. Rodriguez-Wallberg
Summary: Experimental study showed that secondary follicles isolated from ovaries of mice treated with gonadotropins could be cultured in vitro and mature, with development and maturation abilities similar to those from unstimulated controls.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cell & Tissue Engineering
Sureerat Khunmanee, Hansoo Park
Summary: In vitro ovarian follicle culture is a reproductive technique used to overcome infertility issues caused by premature ovarian failure. Three-dimensional culture systems are more suitable for maintaining follicle architecture and have shown great potential in supporting follicle development. Various methods and materials have been studied for their effectiveness in supporting follicular growth, providing opportunities for further advancements in the field.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Mengxue Zheng, Jesus Cadenas, Susanne Elisabeth Pors, Tasnim Esa, Stine Gry Kristensen, Linn Salto Mamsen, Cristina Subiran Adrados, Claus Yding Andersen
Summary: This study aimed to optimize the culture conditions for murine preantral follicles to improve their growth and survival. The results showed that altering the alginate concentration, adding estradiol, and increasing follicle-stimulating hormone concentration can promote the growth and development of preantral follicles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Obstetrics & Gynecology
F. Vitale, L. Cacciottola, F. S. Yu, M. Barretta, C. Hossay, J. Donnez, M. M. Dolmans
Summary: There is a difference in viability and quality of human follicles cultured in ovarian cortex between 20% and 5% oxygen tension. The viability and quality of follicles are higher under 5% oxygen tension after 6 days of in vitro culture (IVC).
HUMAN REPRODUCTION
(2023)
Article
Endocrinology & Metabolism
Nina Desai, Maribeth Spangler, Vaani Nanavaty, Arsela Gishto, Alyssa Brown
Summary: This study investigates the use of a novel tyramine-linked hyaluronan for 3-D in vitro follicle culture. The results show that hyaluronan gel can support the development of individual follicles and ovarian tissue fragments containing follicle clusters. The addition of ECMs or laminin to the gel can enhance certain reproductive outcomes. This extracellular matrix-based 3-D culture model provides a new approach for culturing functionally mature oocytes.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Obstetrics & Gynecology
Johanne Grosbois, Emily C. Bailie, Tom W. Kelsey, Richard A. Anderson, Evelyn E. Telfer
Summary: In vitro culture alters the composition and architecture of the human ovarian cortical extracellular matrix (ECM). The ECM varies in different layers of the ovarian cortex and undergoes remodeling during culture. Mechanical loosening during tissue preparation for culture leads to undefined modifications in the ECM network and alters cell-cell contact, resulting in spontaneous follicle activation.
HUMAN REPRODUCTION
(2023)
Article
Agriculture, Dairy & Animal Science
Ernando I. T. de Assis, Venancia A. N. Azevedo, Miguel F. F. De Lima Neto, Francisco C. C. Costa, Lais R. F. M. Paulino, Pedro A. A. Barroso, Mariana A. M. Donato, Christina A. A. Peixoto, Alane P. O. Do Monte, Maria H. T. Matos, Alana N. N. Godinho, Jordania M. O. Freire, Ana L. P. S. Batista, Jose R. V. Silva, Anderson W. B. Silva
Summary: This study evaluated the potential of Cimicifuga racemosa extract to reduce the harmful effects of doxorubicin on oocytes, follicles, and stromal cells in mice ovaries cultured in vitro. The results showed that doxorubicin reduces the percentage of normal follicles and the density of stromal cells, but these effects were blocked by the extract. Ovaries cultured with the extract had higher levels of mRNA for antioxidant enzymes.
Article
Biochemistry & Molecular Biology
Aleksandra Serafin, Mario Culebras, Maurice N. Collins
Summary: Tissue engineering is proposed as a potential solution to organ shortages. Various hydrogel formulations have been studied, including organic and biocompatible materials that mimic the native tissue. However, comparing studies is difficult due to differences in material concentrations and preparation methods. The current study aims to investigate popular biomaterials and their behaviors for targeted tissue engineering strategies.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biotechnology & Applied Microbiology
Hossein Nikniaz, Zahra Zandieh, Mohammad Nouri, Neda Daei-farshbaf, Reza Aflatoonian, Mazaher Gholipourmalekabadi, Seyed Behnamedin Jameie
Summary: This study compared different protocols for decellularizing the ovarian scaffold and evaluated follicle survival in an extracellular matrix-alginate scaffold. Results showed successful decellularization in all experimental groups, with the SDS-Triton-Ammonium group showing the lowest residual DNA content and highest cytobiocompatibility for follicles. The decellularized scaffold made from ovarian tissues decellularized using SDS-Triton-Ammonium and sodium alginate is suggested as a potential 3D substrate for in vitro culture of follicles for fertility preservation.
Article
Obstetrics & Gynecology
Daniel Shai, Sarit Aviel-Ronen, Itai Spector, Hila Raanani, Moran Shapira, Itai Gat, Hadassa Roness, Dror Meirow
Summary: The study revealed a significant increase in primordial follicle loss and a rise in the number of growing follicles in ovaries treated with alkylating agent chemotherapy. Additionally, a decrease in nuclear FOXO3A localization in primordial follicle oocytes was observed in these ovaries removed within an ultrashort time interval. The findings suggest a pathological mechanism of follicle activation contributing to chemotherapy-induced follicle loss in patients receiving alkylating agent treatment.
FERTILITY AND STERILITY
(2021)
Article
Obstetrics & Gynecology
Jesus Catienas, Liv la Cour Poulsen, Linn Salto Mamsen, Claus Yding Andersen
Summary: In several mammalian species, oocytes from small antral follicles after in vitro maturation (IVM) are successfully used for procreation. Humans are the exception, mainly due to limited access to immature oocytes and unique regulation of oocyte maturation. However, recent findings suggest that human IVM can be developed, with factors such as oocyte diameter, follicle size, hormone levels, and cumulus-oocyte complex secretion influencing the maturation process. Furthermore, studies on signal transduction pathways and hormone levels during physiological conditions provide new insights for improving the current IVM platform. With continued research efforts, it is envisioned that a human IVM platform equally effective as in other mammalian species can be achieved. (Fertil Sterile 2023;119:550-9. (c) 2023 by American Society for Reproductive Medicine.)
FERTILITY AND STERILITY
(2023)
Review
Biochemistry & Molecular Biology
Elsa Labrune, Bruno Salle, Jacqueline Lornage
Summary: This article discusses the progress of in vitro folliculogenesis in humans, highlighting the lack of necessity for initiation activators in the first stage and the requirement for mechanical and/or enzymatic isolation of secondary follicles in the second stage. The need for further validation of this technique is also addressed.
Review
Medicine, Research & Experimental
Hanne Vlieghe, Ellen C. R. Leonel, Parinaz Asiabi, Christiani A. Amorim
Summary: Theca cells play multiple roles during folliculogenesis but their recruitment process and function are not well understood. This review aims to discuss the recent advancements in the characterization and origins of theca cells, their applications in vitro culture for fertility preservation and studies, and their importance in understanding pathogenic pathways and hormonal replacement therapies. Isolation and in vitro culture techniques have improved the understanding of theca cells as a specific cell population. Unraveling the origins of theca cells in the adult ovary will assist in developing hormonal replacement therapies and treating diseases influenced by theca cells.
Article
Obstetrics & Gynecology
Cristina Subiran Adrados, Jesus Cadenas, Mengxue Zheng, Sofie Lund, Elisabeth Clare Larsen, Mette Honnens Tanvig, Vinnie Hornshoj Greve, Paul Blanche, Claus Yding Andersen, Stine Gry Kristensen
Summary: The study found that supplementing the culture media with human platelet lysate significantly increased the survival probability and growth of isolated human pre-antral follicles compared to the use of fetal bovine serum, human serum albumin, or umbilical cord plasma. Additionally, the expression of follicular genes varied among the different groups.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Obstetrics & Gynecology
Su Mi Kim, Jin Young Choi, Myeong Reun Rhee, Seung Hwa Hong, Il Woon Ji, Eun-Hwan Jeong, Jung Ryeol Lee
Summary: This study explores the awareness, intentions, and attitudes of female medical staff towards planned oocyte cryopreservation. The results show that while there is widespread awareness about the procedure, correct knowledge about the age at which female fertility declines is lacking among the participants. The study also reveals a low willingness to consider oocyte cryopreservation among female medical staff, with lack of information being a major obstacle. Furthermore, the study highlights the belief among participants that planned oocyte cryopreservation is a woman’s right, but the lack of access to medical education on fertility preservation is evident.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Engineering, Biomedical
Chungmo Yang, Nanum Chung, Chaeyoung Song, Hye Won Youm, Kangwon Lee, Jung Ryeol Lee
Summary: This study proposes a technique of using nitric oxide-releasing nanoparticles (NO-NPs) and fibrin hydrogels for ovarian transplantation to prevent ischemic damage and promote angiogenesis, resulting in improved quantity and quality of transplanted ovaries.
Article
Obstetrics & Gynecology
Yeon Hee Hong, Seul Ki Kim, Jung Ryeol Lee, Byung Chul Jee, Chang Suk Suh
Summary: The purpose of this study was to determine an optimal maturation method to increase the yield of mature oocytes, focusing on cancer patients who have limited chances for fertility preservation before receiving gonadotoxic therapy. The results showed that dual triggering can be an efficient method to maximize the yield of mature oocytes in breast or endometrial cancer patients using letrozole combined with a GnRH antagonist protocol for fertility preservation.
REPRODUCTIVE MEDICINE AND BIOLOGY
(2022)
Review
Obstetrics & Gynecology
Ae Ra Han, Dayong Lee, Seul Ki Kim, Chang Woo Choo, Joon Cheol Park, Jung Ryeol Lee, Won Jun Choi, Jin Hyun Jun, Jeong Ho Rhee, Seok Hyun Kim
Summary: Based on laboratory and clinical data, it is recommended to administer COVID-19 vaccines to individuals undergoing ART treatment and pregnant women. The vaccines do not have adverse effects on embryos, pregnant women, and breastfeeding infants, and can deliver antibodies through breastfeeding.
CLINICAL AND EXPERIMENTAL REPRODUCTIVE MEDICINE-CERM
(2022)
Article
Medicine, General & Internal
Yeon Hee Hong, Jung Ryeol Lee
Summary: Ovarian tissue cryopreservation and transplantation is a feasible option for fertility preservation, but challenges regarding cryoinjury, ischemic injury, and cancer cell reimplantation need to be addressed.
JOURNAL OF THE KOREAN MEDICAL ASSOCIATION
(2022)
Article
Biochemistry & Molecular Biology
Hui-Jeong An, Sung-Hwan Cho, Han-Sung Park, Ji-Hyang Kim, Young-Ran Kim, Woo-Sik Lee, Jung-Ryeol Lee, Seong-Soo Joo, Eun-Hee Ahn, Nam-Keun Kim
Summary: This study investigated the genetic association between recurrent pregnancy loss (RPL) and microRNA (miRNA) polymorphisms in miR-10aA>T, miR-30cA>G, miR-181aT>C, and miR-499bA>G in Korean women. The study identified specific genotypes of miR-10a and miR-499 that are associated with increased RPL risk.
Article
Obstetrics & Gynecology
Hye Gyeong Jeong, Seul Ki Kim, Jung Ryeol Lee, Byung Chul Jee
Summary: The study assessed the correlation between serum anti-Mullerian hormone (AMH) levels measured by two automated methods and oocyte number in fresh stimulated in vitro fertilization (IVF) cycles. Both methods showed good performance in predicting poor and high responders. The Access method generally yielded higher measured serum AMH levels compared to the Elecsys method.
CLINICAL AND EXPERIMENTAL REPRODUCTIVE MEDICINE-CERM
(2022)
Article
Obstetrics & Gynecology
Seul Ki Kim, Haerin Paik, Jung Ryeol Lee, Byung Chul Jee
Summary: This study found that repeated ejaculates from patients with cancer had stable SDF levels.
CLINICAL AND EXPERIMENTAL REPRODUCTIVE MEDICINE-CERM
(2022)
Review
Obstetrics & Gynecology
Jihyun Kim, Jaewang Lee, Jin Hyun Jun
Summary: The ultimate goal of human assisted reproductive technology is to achieve a healthy pregnancy and birth by selecting and transferring competent embryos. Currently, techniques such as time-lapse imaging systems and deep learning technology have been used to evaluate the state and quality of embryos. In addition, analyzing molecular markers in spent embryo culture media and using non-invasive evaluation methods have shown promising results. However, further clinical trials are needed to confirm these methods.
CLINICAL AND EXPERIMENTAL REPRODUCTIVE MEDICINE-CERM
(2022)
Article
Obstetrics & Gynecology
Inyoung Kang, Myoungjoo Koo, Hyejin Yoon, Beom Seok Park, Jin Hyun Jun, Jaewang Lee
Summary: Monospermy refers to the fusion of a single sperm with an egg during the process of fertilization. Ovastacin, an enzyme involved in breaking down the egg's outer layer, plays a crucial role in preventing polyspermy and ensuring successful fertilization.
CLINICAL AND EXPERIMENTAL REPRODUCTIVE MEDICINE-CERM
(2023)
Article
Environmental Sciences
Myoungjoo Koo, Inyoung Kang, Jin Hyun Jun, Jaewang Lee
Summary: This study investigated the effects of TBBPA on infertility and early implantation events in the uterine endometrium. The findings showed that TBBPA induced an inflammatory response in gene expression and inhibited embryonic outgrowth. However, TBBPA had minimal effects on protein levels and embryo attachment rate.
ENVIRONMENTAL SCIENCES EUROPE
(2023)
Article
Oncology
Yeon Hee Hong, Changhee Park, Haerin Paik, Kyung-Hun Lee, Jung Ryeol Lee, Wonshik Han, Seho Park, Seockhoon Chung, Hee Jeong Kim
Summary: Fertility preservation is a major concern for breast cancer patients undergoing multimodality treatment. Cryopreservation of oocytes, embryos, and ovarian tissue are currently available methods, and in vitro maturation or gonadotropin-releasing hormone agonist could also be considered. Good communication with patients in the decision-making process is essential, and timely referral to fertility specialists for individualized treatment is crucial for desirable outcomes. A multi-disciplinary team-based approach and in-depth discussion on breast cancer treatment and fertility preservation is necessary.
JOURNAL OF BREAST CANCER
(2023)
Article
Medicine, General & Internal
Su Mi Kim, Kyu Hee Won, Yeon Hee Hong, Seul Ki Kim, Jung Ryeol Lee, Byung Chul Jee, Chang Suk Suh
Summary: This study found that microorganisms can be present in the follicular fluid and vaginal secretions of infertile women. Microorganisms in the follicular fluid were not associated with embryo quality or clinical pregnancy rates, while vaginal pathogens were associated with poorer implantation rates and clinical pregnancy rates in IVF cycles.
YONSEI MEDICAL JOURNAL
(2022)
Article
Medicine, General & Internal
Dahyeon Yoo, Nanum Chung, Jungyoung Yoo, Chae Young Song, Chungmo Yang, Hye Won Youm, Kangwon Lee, Jin Hyun Jun, Jaewang Lee, Jung Ryeol Lee
Summary: This study evaluates the efficacy of tissue-based hormone replacement therapy (tHRT) in treating signs and symptoms of menopause. The results show that tHRT can improve bone density and volume, as well as restore uterine health in post-menopausal women. This suggests that ovarian tissue cryopreservation and transplantation can be used not only for fertility preservation but also for treating sex hormone-deficient post-menopausal women.
YONSEI MEDICAL JOURNAL
(2022)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.
